Occurrence of Helicobacter pullorum in Retail Chicken Meat: A One-Health Approach to Consumer Health Protection.

Helicobacter pullorum is an emerging foodborne pathogen that commonly colonizes the gastrointestinal tract of poultry, causing gastroenteritis. It has been related to several clinically important infections, including colitis and hepatitis, inflammatory bowel disease, recurrent diarrhea, and bacteremia in the human population. The bacterium may be transmitted to humans through undercooked poultry meat. In order to investigate the occurrence of H. pullorum in raw retail chicken meat (thighs and breasts), we analyzed 240 samples: 120 chicken thigh and 120 chicken breast samples. The samples were analyzed by means of an isolation protocol using Steele and McDermott's modified filtration technique on Brucella agar supplemented with 5% of defibrinated sheep's blood. The presumptive colonies were biochemically identified and analyzed using a previously described conventional PCR test based on the 16S rRNA gene. In total, 35% of analyzed samples were positive using the microbiological protocol and 45% were positive by PCR. These results suggest that H. pullorum can be transmitted to humans through the handling and consumption of raw poultry meat, representing a risk for food business operators and consumers. Efforts to control H. pullorum in broiler meat should prioritize the implementation of stringent hygienic practices across all stages of the food chain, from the farm to the consumer.

Effect of rearing system (free-range vs cage) on gut and muscle histomorphology and microbial loads of Italian White breed rabbits.

OBJECTIVE: The growing consumers' interest on animal welfare has raised the request of products obtained by alternative rearing systems. The present study was conducted to assess the influence of housing system on gut and muscle morphology and on microbial load in rabbits reared under free-range (FR) and cage system (CS). METHODS: A total of forty weaned (35 days of age) male Italian White breed rabbits were allotted according to the rearing system, and at 91 days of age were randomly selected and slaughtered for the morphological evaluation of tissue from duodenum and longissimus lumborum. Morphometric analysis of the villus height, villus width, crypt depth, villus height/crypt depth ratio, and villus surface was performed. The microbial loads on hind muscle was determined by total mesophilic aerobic count (TMAC), Escherichia coli and Enterobacteriaceae; whereas, total anaerobic bacteria count (TABC) and TMAC, E. coli and Enterobacteriaceae was determined on caecal content. RESULTS: Rearing system did not interfere with the duodenum and muscle histomorphology in both rabbit groups. Similarly, microbial load of caecal content showed no significant differences on the TABC and TMAC. Conversely, significant difference was found for E. coli strains in caecal content, with the lower counts in FR compared to CS rabbits (p<0.01). Microbiological assay of muscle revealed significant lower TMAC in FR vs CS rabbits (p< 0.05). All rabbit meat samples were negative for E. Coli and Enterobacteriaceae. CONCLUSION: Free-range could be considered a possible alternative and sustainable rearing system in rabbits to preserve gut environment and muscle quality.

Trace Metals in Pork Meat Products Marketed in Italy: Occurrence and Health Risk Characterization.

This study provides valuable information on the levels of various trace metals (Pb, Cd, Hg, Zn, Cu, Cr) in meat products (baked ham, raw ham, mortadella, cured sausage, würstel, salami) from South Italy and calculates potential health risk toxicity associated with their consumption for the total population and for children. In the samples studied metal concentrations are within the permissible legal limits (Cd: 0.01-0.03 µg g-1 w.w., Hg: 0.01-0.02 µg g-1 w.w., Zn: 5.71-7.32 µg g-1 w.w., Cu: 1.08-1.21 µg g-1 w.w., Cr: 0.15-0.23 µg g-1 w.w.), except for Pb (Pb: 0.22-0.38 µg g-1 w.w.). The estimated intake values are within the provisional tolerable daily intake limits for toxic metals and recommended daily intake values for essential metals in both tested groups. The noncarcinogenic risk values of the individual metals indicate that there is no health risk, but their combined effects might constitute a potential risk for children. Furthermore, the cumulative cancer risk of all samples studied exceeds the recommended threshold risk limit (> 10-4) in both total population and children, indicating a risk of potential health problems for consumers especially for children, who are more vulnerable to toxic metal exposure.

Helicobacter pylori: Survival in cultivable and non-cultivable form in artificially contaminated Mytilus galloprovincialis.

Several studies report the presence of Helicobacter pylori (H. pylori) in seawater either free or attached to planktonic organism. After considering the role played by plankton in the food chain of most aquatic ecosystems and the possible role that seafood products can assume in the transmission of H. pylori to humans, the aim of this study was to assess the survival of H. pylori in artificially contaminated Mytilus galloprovincialis (M. galloprovincialis). A traditional culture method and a reverse transcriptase-PCR (RT-PCR) assay were employed to detect the mRNA of known virulence factor (VacA) which can be considered use a marker of bacterial viability. The obtained results clearly show that H. pylori is able to survive in artificially contaminated mussels for 6 days (2 days in a cultivable form and 4 days in a non-cultivable form).

Dioxin and PCB residues in meats from Italy: Consumer dietary exposure.

In order to investigate PCB and PCDD/F concentrations and potential human health risk a study has been conducted in meat samples (beef, pork, chicken and turkey) purchased from Italian supermarkets. PCBs were dominant (41.8-77.7 ng g-1 l.w.) with respect to PCDD/Fs (20.1-91.1 pg g-1 l.w.). The levels were variable and largely dependent upon the type of meat. Accumulation pattern showed a distribution typically reported for meat. PCBs tended to decrease from lowest to highest congeners, while for PCDD/Fs the concentration profile was dominated by highly chlorinated dioxins and furans. Concentrations of PCDD/Fs plus dl-PCBs and indicator PCBs exceeding the EU maximum permissible levels were found in 23.3% and 53.3% of the samples, respectively. Exposure estimates to PCDD/Fs plus dl-PCBs (0.08-4.16 pg WHO-TEQs kg-1 b.w. w.-1) were within the new limit proposed by the EFSA's expert panel, except for pork sausage, showing a twofold higher value. Concerning the exposure derived from indicator PCBs, the maximum level set by different European countries (10 ng kg-1 b.w. d.-1) was surpassed solely via consumption of pork sausage (17.22 ng kg-1 b.w. d.-1), thought also the estimated intake from hamburger consumption (6.88 ng kg-1 b.w. d.-1) merits attention being close to guideline limit.

Helicobacter pylori: A foodborne pathogen?

Helicobacter pylori (H. pylori) is an organism that is widespread in the human population and is sometimes responsible for some of the most common chronic clinical disorders of the upper gastrointestinal tract in humans, such as chronic-active gastritis, duodenal and gastric ulcer disease, low-grade B-cell mucosa associated lymphoid tissue lymphoma of the stomach, and gastric adenocarcinoma, which is the third leading cause of cancer death worldwide. The routes of infection have not yet been firmly established, and different routes of transmission have been suggested, although the most commonly accepted hypothesis is that infection takes place through the faecal-oral route and that contaminated water and foods might play an important role in transmission of the microorganism to humans. Furthermore, several authors have considered H. pylori to be a foodborne pathogen because of some of its microbiological and epidemiological characteristics. H. pylori has been detected in drinking water, seawater, vegetables and foods of animal origin. H. pylori survives in complex foodstuffs such as milk, vegetables and ready-to-eat foods. This review article presents an overview of the present knowledge on the microbiological aspects in terms of phenotypic characteristics and growth requirements of H. pylori, focusing on the potential role that foodstuffs and water may play in the transmission of the pathogen to humans and the methods successfully used for the detection of this microorganism in foodstuffs and water.

PCBs and PCDD/Fs in Bluefin Tuna: Occurrence and Dietary Intake.

Polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-furans (PCDD/Fs) were measured in Mediterranean bluefin tuna (Thunnus thynnus) to verify the compliance with the EU regulations for food commercialization. The estimated intakes were also evaluated. The analyses were performed by gas chromatography-ion trap tandem mass spectrometry (GC-MS-MS). The PCBs were dominant (1132.0 ng g−1 l.w.), followed by PCDFs (23.2 pg g−1 l.w.) and PCDDs (8.5 pg g−1 l.w.). The pollutant levels (dl-PCBs: 0.7 pg TEQ/g w.w.; PCDD/Fs: 1.9 pg TEQ/g w.w.) and their sum expressed as TEQ values (2.6 pg TEQ/g w.w.) remained below the limits for human consumption proposed by the European Union. On the contrary, the sum of the six indicator non-dioxin-like PCBs (84.2 ng g−1 w.w.) was slightly above the maximum level fixed by the in-force legislation. The estimated dietary intakes for PCDD/Fs plus dl-PCBs were below the toxicological reference values (TRVs) set by various international bodies, while non-cancer and cancer risk assessment revealed a safety concern. Additionally, the estimated intake of ndl-PCBs exceeded the maximum levels set by different European countries. These findings suggest caution in tuna consumption together with an active and frequent surveillance of the chemical quality of its flesh.