[Using atomic force microscopy to analyze morphological changes and mechanical properties caused by cellular exposure to low doses of pesticides]. / Utilizzo della microscopia a forza atomica per l'analisi di alterazioni morfologiche e meccaniche cellulari causate da esposizione a basse dosi di pesticidi.

A commercial pesticide is usually composed of active ingredients and formulants. Among the active ingredients, Deltamethrin is a pyrethroid chemical widely used for synthesizing pesticides products which are very effective in damaging the central nervous system of pests. In this work, we analyze, by means of atomic force microscopy (AFM), cellular morphological changes induced by exposure to a Deltamethrin-based commercial pesticide (Decaflow). AFM microscopy, in addition to the well-known characterization of the cellular topography, has the ability to monitor interesting biomechanical parameters of the surface as roughness and elastic modulus. In particular, we exposed normal human keratinocytes for 24 hours at different solutions of Decaflow, well below the threshold of cytotoxicity. The AFM images of exposed cells show alterations of surface cell shape. Moreover exposed cells are characterized by an increase of the value of membrane roughness. The mechanical properties of cells are also modified after Decaflow exposure, as confirmed by a decrease of the elasticity modulus with increasing the concentration of pesticide.

[Epidemiological study on tremor in the aged]. / Studio epidemiologico sul tremore della terza età.

203 hospitalized old-aged patients were examined for the presence of tremor. This physical sign was found in 58.6% of cases. By far the largest diagnostic category resulted that of essential tremor: 46.7% of all patients investigated and 77.8% of all cases of tremor. The frequency of this type of tremor was found to increase proportionally to age. The conclusion is drawn that also tremor, as well as other neurological signs frequently demonstrable in "normal" old people (for example, the so-called primitive reflexes), should be inscribed in the group of the phenomena due to the ageing process itself.

[Comparative study of the frequency of 6 primitive reflexes in a hospitalized population of aged patients]. / Studio comparativo della frequenza di 6 riflessi primitivi in una popolazione ospedalizzata di età geriatrica.

110 elderly patients, 57 with and 53 without acquired neurological lesions, were examined for the presence of the grasping, snout, sucking, palmomental, glabellar and bulldog reflexes. The results were statistically analyzed by means of the chi2 method. The percentages of the positive reflex responses, although not negligible in the neurologically healthy patients, turned out far higher in the patients with organic cerebral disorders. With the exception of the sucking and palmomental reflexes, these differences appeared statistically significant. As for the neurological diagnosis, diffuse lesions proved themselves more crucial than focal ones. The conclusion is drawn that primitive reflexes should be regarded abnormal only in the context of an overt cerebral pathology.