RESUMO
The study was carried out on 175 patients who underwent laparoscopy together with search for chlamydia in the peritoneal fluid when they were having investigations for sterility or for pelvic pain. These patients are classified into three groups according to the clinical and laparoscopic features: 50 cases of acute salpingitis. 104 cases of tubal sterility and 21 control cases who were normal on laparoscopy. The mean age was 25.7 years for acute salpingitis and 30.4 years for tubal sterility. The purpose of this study was to work out the role played by chlamydia trachomatis in tubal phatology and two techniques were used at the same time: The identification of chlamydia trachomatis in 175 samples of peritoneal fluid taken during a laparoscopy; Research for anti-chlamydia antibodies in serum using an indirect micro-immunofluorescent technique. Cell cultures were performed after the peritoneal fluid had been centrifuged. It had been in transport medium 2 SP and frozen. Our technical methods for isolating chlamydia (microplaque culture on Hela lines, incubation in the presence of cycloheximide and research for chlamydial inclusion bodies using direct immunofluorescence with monoclonal antibodies and 2 successive passages of the cells) have allowed us to confirm that chlamydia was present in 44% of cases of acute salpingitis and in 37% of cases of tubal sterility. The search for anti-chlamydial antibodies in the serum in indirect immunofluorescence with a single antigen (of L2 serotype) gave positive results which were comparable to those in other studies, i.e. 50% of positive serological results in acute salpingitis and 63% in tubal sterility.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Líquido Ascítico/microbiologia , Infecções por Chlamydia/complicações , Infertilidade Feminina/microbiologia , Salpingite/microbiologia , Doença Aguda , Adulto , Chlamydia trachomatis , Feminino , HumanosRESUMO
We reported 277 clinical cases of Leptospirosis with a significant serological title (greater than 100) by lyse-agglutination. Sera were sampled from 1972 to 1984 among 9182 subjects living in Central-Eastern departments of France) for immunological diagnosis of leptospirosis. We noted the stability of the average yearly rate in this region, the difficulty in evaluating the incidence of the disease, the clinical predominance of icterus and the good clinical prognosis with specific antibiotherapy.
Assuntos
Leptospirose/epidemiologia , França , Testes de Hemaglutinação , Humanos , Leptospirose/imunologia , Programas de Rastreamento , Doença de Weil/epidemiologia , Doença de Weil/imunologiaRESUMO
91 rats captured in 1982 in the sewers of Lyon (France) have been examined for the presence of some microorganisms implicated in infections transmissible to man, by direct examination, bacteriological culture or detection of specific antibodies. Bacteriological results have shown a high proportion of carriers of Yersinia enterocolitica and frederiksenii (29%), of Pasteurella pneumotropica (29%), of Staphylococcus aureus (53%), and of Campylobacter jejuni (18%), a low proportion of carriers of Salmonella typhimurium (6%) and Leptospira (7%), and an absence of Listeria. Immunological reactions were often positive with Leptospira (21%), sometimes with Salmonella typhimurium, and always negative with Yersinia and Listeria. This work is a contribution to the knowledge of the health status of sewer rats considered to be an important public health risk as transmitters of anthropozoonoses.