RESUMO
In this study, we used an alginate-gelatin bioink to design and print 3D constructs with lattice, honeycomb and fibrous bundle patterns. These designs were printed using a small-scale laboratory printer, at first and later translated to a larger scale, high throughput-printing platform. A comparative analysis of the structures printed using two dissimilar platforms using gross morphologic evaluation, scanning electron microscopy and swelling assay confirmed our hypothesis that a design printed using a small-scale laboratory bioprinter for optimization of bioink composition and printing parameters can be successfully translated into a large scale-printing platform for high throughput printing of constructs. Since the designs for printing were implemented using a software which was common across both printers, this endpoint was feasible. The only difference in printing parameters resulted from variation in extrusion pressure which was due to a significant difference in barrel size used across both printers (3 ml versus 30 ml), while all other parameters stayed the same. Although the scaffolds were not bioprinted with cells, in future we will investigate how cell viability can be differentially regulated by the variation of extrusion pressure across both platforms.
RESUMO
La Escuela Técnica de Salud Boliviano Japonesa de Cooperación Andina es una institución dependiente del Ministerio de Previsión Social y Salud Pública, la cual fue construída por el gobierno japones, para la formación de recursos humanos de nivel técnico para la salud. La construcción y el equipamiento de Escuela finalizó en el mes marzo de 1992 y la entrega oficial de la misma se llevó acabo el 23 de abril del mismo año: Uno de los fines mas importantes es el de formar recursos humanos para la salud preferentemente de nivel medio y auxiliar, en concordancia con las necesidades del país..