Flow-Cytometry in the Diagnosis of Diffuse Large B-Cell Lymphoma Based on Stomach Tissue Samples: A Case Report.

Morphology and immunohistochemistry on node, tissue, and bone marrow biopsies are frequently used in lymphoma diagnosis to characterize the stage and subtype of diseases. Multicolor flow cytometry technology is a novel technique for the analysis of immunological markers to identify lymphoma on fresh tissue when immunohistochemical staining is ambiguous. We report a case of a patient diagnosed with diffuse large B-cell lymphoma by flow cytometry on a stomach tissue biopsy.

Effects of solvent-solvent fractionation on the total terpenoid content and in vitro anti-inflammatory activity of Serevenia buxifolia bark extract.

Severinia buxifolia (Rutaceae) is often used as a traditional medical plant. The present study was carried out to estimate the effects of solvents (petroleum ether and hexane: ethyl acetate) used in liquid-liquid extraction to total terpenoid content (TTC) and in vitro anti-inflammatory activity of the extracts obtained from S. buxifolia bark. The results showed that solvent fractionation increased the TTC compared with crude extracts. The hexane: ethyl acetate bark extract fraction (HEF) had the highest TTC (731.48 µg/ml) in comparison with the petroleum ether bark extract fraction (PEF) (564.81 µg/ml) and the crude extract (CE) (184.26 µg/ml). In addition, one of composition of terpenoid of S. buxifolia, namely ursolic acid, was determined by HPLC method from the crude CE and the fractions PEF and HEF: 2.44 µg/g DW, 3.56 µg/g DW and 5.04 µg/g DW, respectively. The samples had an in vitro anti-inflammatory activity comparable with that of two reference standards (aspirin and indomethacin). Particularly, the HEF fraction had the highest in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 147.91 µg/mL, heat-induced hemolysis: IC50 = 159.91 µg/mL, proteinase inhibition: IC50 = 117.72 µg/mL, and lipoxygenase activity: IC50 = 90.45 µg/mL). Besides, the preliminary experiments of this study were conducted to determine the influences of maceration factors (solvent type, temperature, and time) for S. buxifolia bark extract. The TTC ranged from 453.70 to 842.59 mg linalool/g DW, and the extraction yield from 2.40% to 5.120% in all extracts. Based on TTC and EY, the hexane: acetone mixture is recommended as the optimal solvent to obtain the crude bark extract (CE) at 46°C for 24 hr of maceration. Extracts of S. buxifolia bark are a promising source for the treatment of inflammatory diseases.

Paraneoplastic Cast Nephropathy Associated With Pancreatic Mixed Acinar-Neuroendocrine Carcinoma: A Case Report.

Acute kidney injury is common in patients with cancer and may result from sepsis, obstruction, radiotherapy, chemotherapeutic agents, and nonsteroidal anti-inflammatory drugs. Rare reports of acute kidney injury due to cast nephropathy in patients with pancreatic acinar cell carcinoma have been described, but a pathogenetic link between cast nephropathy and carcinoma was not established. We report a patient with pancreatic mixed acinar-neuroendocrine carcinoma who developed severe acute kidney injury. Kidney biopsy showed cast nephropathy characterized by fractured periodic acid-Schiff-negative casts, associated with mononuclear and giant cell reaction. The patient did not have multiple myeloma and casts did not show immunoglobulin light chain restriction on immunofluorescence. Analysis using liquid chromatography-tandem mass spectrometry and immunohistochemistry identified 2 acinar cell-specific proteins, regenerating islet-derived 1α and carboxypeptidase A1, in both tubular casts and tumor cells. Thus, this case demonstrates that solid tumor-specific proteins can be nephropathic by obstructing renal tubules, resulting in acute kidney injury, a previously proposed but not characterized pathophysiologic mechanism for paraneoplastic nephropathy associated with carcinoma.

An actinoporin plays a key role in water stress in the moss Physcomitrella patens.

* Modern land plants arose from a green algae-like ancestor c. 480 million years ago. While several novel morphological features were critical for survival in the aerial environment, physiological innovation undoubtedly played a key role in the colonization of terrestrial habitats. Recently, actinoporin genes, a small group of pore-forming toxins from sea anemones, have been found in the bryophyte and lycophyte lineages of land plants where they are upregulated in water-stressed tissues. * The bryoporin gene in the moss Physcomitrella patens (PpBP) was functionally characterized by RNA blot analyses and overexpression in P. patens. In order to examine functional homology between PpBP and sea anemone actinoporins, the recombinant PpBP was subjected to hemolytic analysis of pig blood cells, which is one of the specific activities of actinoporins. * PpBP was upregulated by various abiotic stresses, in particular most strongly by dehydration stress. Overexpression of the bryoporin gene heightens drought tolerance in P. patens significantly. In addition, PpBP shared the highest structural homology with actinoporins in a three-dimensional structural database and showed hemolytic activity. * These results suggest that this phylogenetic distribution may have resulted from an ancient horizontal gene transfer and actinoporins may have played an important role in early land plants.

Proteome analysis of gametophores identified a metallothionein involved in various abiotic stress responses in Physcomitrella patens.

Physcomitrella patens is a model plant for studying gene function using a knockout strategy. To establish a proteome database for P. patens, we resolved over 1,500 soluble proteins from gametophore and protonema tissues by two-dimensional electrophoresis (2-DE) and obtained peptide mass fingerprints (PMFs) by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS). Using expressed sequence tags (ESTs), we were able to predict the identities of 90 protein spots. Most of these were related to energy or primary metabolism. Comparative proteome analysis was used to identify proteins specific for each of the tissue types. One of these was a metallothionein type-2 (PpMT2) protein that was highly upregulated in gametophore tissue. PpMT2 was induced in both the gametophore and protonema following culture on solid media and in response to various abiotic stresses such as copper, cadmium, cold, indole-3-acetic acid, and ethylene. We suggest that PpMT2 is not only involved in metal binding and detoxification, but also in many biological aspects as a metal messenger or a protein with additional functions.

Some characteristics of private health services’ users in Hanoi and Danang

The research on using private health services of 400 users in 500 private clinics in Hanoi and Danang showed that average age of users is 38.3. College and university education levels are 26.8%. Most users get the medium living standard 58%, the poor is under 10%. Structural of diseases of users are mainly dento-oral diseases, ear-nose-throat diseases, eyes diseases, and then are respiratory diseases and other normal diseases such as cold, runny nose, headache

Evaluation on the efects of Livcol in patients with chronic hepatitis B

The study aimed to analyse the effects of Livcol, a herbal medication, on chronic hepatitis B. The patients were divided into two groups: the study group using Livcol, the control group using Fortec for 1 months. Results: Almost clinical menifestations reduced or disappeared, transaminase level was lowered equally in both groups with statistic significance. There was 1 case of seroconversion of HBeAg seen in both group. Livcol showed no adverse effects in this trial

The cerebral protective effect of a medicinal formulation in mice with ischemic/reperfusion induced brain injuries

An animal model to initiate anl oxidative stress in brain has been successfully applied in vitro condition. Temporary brain ischemia is conducted by two carotid arterie occlusion for 20 min. Then a reperfusion is followed for 20 min. aiming to produce oxidative damage. MDA measurements are used to assess the levels of brain damage and protective effects of studied extracts. One formulation for "activating the blood and clearing the obstruction" has been tested for the protective activity against the ischemia/repelfusion-induced injuries to brain. The extract was given to mice in doses of 200, 300 and 500mg/kg for 10 continuous days prior to the induction of brain ischemia. This application showed a remarkable protective effects by limiting the elevated MDA levels in brain homogenates, resulted from ischemia/reperfusion (P<0.001). Corresponding to the increased oxidative stress in ischemia/reperfusion, SOD activity (an antioxidant enzyme) was found to be reduced in this model, while the studied extract almostly reversed the decrease of SOD activity when it was given to mice in the dose of 500 mg/kg body weight

The indispensability, objectiveness and science of the integration of modern and traditional medicines

Having the common objectives is human being – health, modern medicine should coordinate with traditional medicines in order to promote ability of medicines in protecting people’s health. Combination of these two major medicines is the high step of inheritance process, as it is necessary to select the quintessence, good features and to reject the backwardness, superstition and harmfulness to human being. This combination process happens naturally, popularly in society and medicine. This is an appropriate, stable development and it will be seen as a right choice in the future

Research on the clinical effects of "Trinh nu hoang cung" capsules in the treatment of benign postage hypertrophy

A prospective study was conducted to evaluate and compare the results before and after using “Trinh nu hoang cung” capsules in 52 patients who had benign enlargement of prostate. Results: “Trinh nu hoang cung” capsules had effectiveness in the treating of urination disorders in patients with benign postage hypertrophy; with the rate of moderate and good results is 96.1%. The capsule had effect of clearing the urinary, reducing urination disorder, reducing the saving urine volume (from 11.86 ml to 4.3 ml). It did not cause any change in index of physiology, blood, biochemistry, or function of liver, kidney, circulation, blood, as well as did not have any side effect in digestive tract and central nervous system

Traditional Medicine in the treatment of headache

According to the traditional medicine, headache was due to exopathy or internal injury. Hereafter was the treatment of headache based on the differential diagnosis: Headache (H) due to wind-cold pathogen, treatment is relieving the exterior syndrome with drugs pungent in flavor and warm in property, expelling wind and cold pathogens; H due to pathogenic wind-heat, treament is relieving the exterior syndrome with drugs pungent in flavor and cool in property, expelling wind and clearing away heat; H due to pathogenic wind-dampness, treatment is dispersing pathogenic wind, resolving dampness. H due to hyperactivity of the liver-yang, treatment is calming the liver, suppressing yang hyperactivity of the liver. H due to phlegm-dampness, treatment is resolving phlegm; H due to the kidney-yin (or-yang) deficiency, treatment is nourishing yin (or yang) and supplenenting the kidney; H due to deficiency of qi, treatment is reinforcing the spleen to replenish qi; H due to deficiency of blood, treatment is nourishing the blood, alleviating pain; H due to blood stasis, treatment is removing blood stasis to promote blood circulation, alleviating pain. The diagnosis and treatment of the three forms of migraine were also reported

Some remarks of private medical practice in Ha Noi in practicing the Decree on Private Medical Practices of Government

An inquiry was performed on 280 private institutions of health services in 4 districts of Ha Noi city concerning the analysis of their adminiotrative state and activities. Results showed that in 96.27% of these institutions, there are adequate documents of principal occupational law, in 87.2% - adequate records and statistics, 85.64% - posting up for professional scopes, in 91.46% posting up for service price, in 92.35% proper posters… Legitimate aspirations of the private manager for updating the knowledge, for insurance in case of occupational risk, for health insurance, for borrowing … were concerned

South korean traditional medicine

Combination of Chinese Traditional medicine and Korean popular medicine. Since year 1952, the Korean Traditional medicine Association has been recognized by the Government and since the year 1953, Traditional medicine College (Kyung Hee University) was established. Up to now, there are 11 Traditional medicine Colleges in South Korea. Anually 500 doctors are graduated. In the Kyung Hee University and Wont-Wang University, traditional pharmacists specialized by material medical were graduated. In the whole country there are 115 Traditional medicine Hospitals and 6670 clinics and consultation buros of Traditional medicine. The Korean Association of Traditional medicine Hospital was established in the year 1988, and the Institute of traditional research in 1994