RESUMO
An enzymatic fluorescent probe is developed for the selective detection of glucose. In this work, a Bovine Serum Albumin stabilized gold nanocluster (BSA-AuNCs) was synthesized by microwave assisted method, and it is modified with glucose oxidase, thereby a fluorescent enzymatic sensor (BSA-AuNCs@GoX) was designed for the sensitive detection of glucose with a limit of detection of 0.03â mM. The red fluorescence exhibited by the probe is quenched by the production of H2O2 on addition of glucose via. a static quenching mechanism from UV visible absorption and Fluorescence lifetime results. The developed probe exhibits good selectivity and sensitivity with other coexisting molecular species such as glycine, creatinine, methionine, histidine, uric acid, albumin, and ions such as sodium, potassium, calcium, magnesium, zinc etc. that appear in the body fluid. The practical applicability was studied in paper strip and extended its reproducibility in biological matrixes such as human serum and urine and found a good recovery percentage of 94-101 %. By this way, we have fabricated an effective fluorescent enzymatic "turn-off" sensing probe for the detection of glucose.
Assuntos
Corantes Fluorescentes , Glucose Oxidase , Glucose , Ouro , Nanopartículas Metálicas , Soroalbumina Bovina , Ouro/química , Glucose Oxidase/química , Glucose Oxidase/metabolismo , Soroalbumina Bovina/química , Nanopartículas Metálicas/química , Animais , Humanos , Bovinos , Glucose/análise , Glucose/química , Corantes Fluorescentes/química , Técnicas Biossensoriais/métodos , Limite de Detecção , Espectrometria de Fluorescência , Glicemia/análiseRESUMO
The near-unity triplet quantum yield of photoexcited carbonyl functionalized pyrenes is theoretically investigated. The estimated energetics of singlet-triplet manifolds and relevant spin-orbit coupling parameters strongly suggest triplet state formation via the S1â T4/T5 pathway. Quantum wavepacket dynamics of triplet manifolds within the linear vibronic coupling approach reveal that the receiver triplet state would undergo rapid internal conversion decay to the lower triplet state(s), facilitating efficient triplet generation by minimizing the reverse intersystem crossing possibilities. On the basis of these results, a unified mechanism is proposed to describe the ultrafast intersystem crossing process in these molecules.