An In Situ Fabricated Hydrogel Polymer - Palladium Nanocomposite Electrocatalyst for the HER: Critical Role of the Polymer in Realizing High Efficiency and Stability.

Development of general and simple designs of catalytic electrodes for the hydrogen evolution reaction (HER) is critical. The present work demonstrates the multiple roles played by a hydrogel polymer in the fabrication and activity enhancement of the nanoelectrocatalyst. A nanocomposite thin film of Pd with the insulating hydrogel, poly(2-hydroxyethyl methacrylate) (PHEMA), is fabricated through a facile in situ process, the polymer itself functioning as the reducing/stabilizing agent in the formation of Pd nanoparticles. Pd-PHEMA on Ni foam enables efficient HER in alkaline medium with a low overpotential; the polymer enables the electrocatalysis by its swelling and confinement of the electrolyte. Most significantly, when the electrode is subjected to an optimized cycling protocol, the overpotential decreases steadily, reaching an impressively low value of 36 mV (@10 mA cm-2 ). A low Tafel slope (68 mV dec-1 ), high exchange current density, Faradaic efficiency and TOF (3.27 mA cm-2 , 99 %, 122.7 h-1 ), and extended stability are achieved. Detailed investigations reveal the active role of the polymer in the evolution of the nanocatalyst, itself undergoing favorable morphological changes. The study illustrates the widened scope for developing efficient and stable catalytic electrodes with hydrogel polymers and unique features that promote the generation of green hydrogen.

Hydrogel Polymer-PBA Nanocomposite Thin Film-Based Bifunctional Catalytic Electrode for Water Splitting: The Unique Role of the Polymer Matrix in Enhancing the Electrocatalytic Efficiency.

A novel approach to efficient bifunctional catalytic electrodes for water splitting is developed, based on a counterintuitive choice of an insulating hydrogel polymer (chitosan, CS)-Prussian blue analogue (PBA, KCoFe) nanocomposite thin film on nickel foam. The polymer matrix in KCoFe-CS enables the formation of framelike structures of the non-noble metal-based catalyst nanocrystals, in addition to improving their stability. An optimized cycling protocol leads to a substantial enhancement of the electrocatalytic efficiency for oxygen evolution reaction (OER) as well as hydrogen evolution reaction (HER), achieving relatively low overpotentials of 272 and 320 mV (@ 10 and 20 mA cm-2) and 146 mV (@ 10 mA cm-2), respectively, reduced Tafel slopes, and increased Faradaic efficiencies of 98 and 96%; the overpotentials estimated based on the electrochemically active surface area show similar trends. The polymer encapsulation and the cycling protocol are key to the realization of the desirable combination of enhanced efficiency and stability demonstrated up to 50 h for both OER and HER. Detailed characterizations of the postcycling catalytic electrode show that favorable morphological changes of the polymer matrix with concomitant reduction in the PBA nanocrystal size lead to the enhanced activity. The bifunctional activity of the catalytic electrode is demonstrated by the stable water splitting achieved with a 20 mA cm-2 current density at 1.55 V. The present study unravels the utility of hydrogel polymer matrices (without the use of binders like Nafion) in realizing sustainable water splitting electrocatalysts with high stability and efficiency, through the combined effect of confining the electrolyte within and favorably modifying the catalyst nanoparticles and the nanocomposite morphology.

Alleviation of Salinity Stress in Peanut by Application of Endophytic Bacteria.

The development of salinity affects 7% of the world's land surface, acting as a major constraint to crop productivity. This study attempted to use the co-evolving endophytes of peanut to alleviate salinity stress and enhance the yield of peanut. Diverse and different tissue colonizing endophytes were isolated from peanut and screened in vitro by seed germination bioassay imposing gradients of salinity, with two cultivars TG37A (susceptible) and GG2 (moderately resistant), in potted conditions using saline irrigation water. Finally, nine endophytes capable of producing IAA and ACC-deaminase, promoting root growth and yield in potted conditions were selected for further evaluation in field conditions. They were evaluated with saline water (1.5-2.0 dS/m) in saline soil with susceptible cultivar TG37A. Simultaneously, three endophytes (Bacillus firmus J22N; Bacillus tequilensis SEN15N; and Bacillus sp. REN51N) were evaluated with two cultivars, GG2 and TG37A, during rainy and post-rainy seasons with elevated salinity. The application of endophytes like Bacillus firmus J22N and Bacillus sp. REN51N enhanced the pod and haulm yield of peanuts by 14-19% across cultivars, salinity, and seasons. In addition, there was significant modulation in parameters like relative water content; production of enzymes like superoxide dismutase (SOD), glutathione reductase (GR), catalase (CAT), ascorbate peroxidase (APX), lipid peroxidase (POD), and H2O2 content in leaf; and uptake of potassium. The activities of the enzymes involved in scavenging reactive oxygen species (ROS) increased with salinity, and further increased with endophytes like Bacillus firmus J22N, Bacillus tequilensis SEN15N, and Bacillus sp. REN51N. There was an enhanced accumulation of proline, reduced level of phenol and H2O2, and enhanced uptake of potassium with the inoculation of endophytes. This improved scavenging capacity of plants by endophytic modulation of ROS scavengers, uptake of K, production of ACC deaminase and IAA, root and biomass growth, modulation in relative water content, and enhanced accumulation of osmoprotectant might be the reasons of alleviation of salinity stress. Endophytes could have alleviated salinity stress in peanuts, indicating the mechanisms and potential of peanuts at the field level. These endophytes could be applied to bring agricultural sustainability to salinity-affected areas in the future. Furthermore, few genera viz. Kocuria, Brevundimonas, Agrococcus, Dietzia, and Kytococcus were observed in peanut tissue for the first time.

Simultaneous bioelectricity generation and water desalination using Oscillatoria sp. as biocatalyst in photosynthetic microbial desalination cell.

Globally, the scarcity of drinking water has triggered the researchers towards the development of desalination techniques to turn up saline water into potable. Microbial Desalination Cell (MDC) is a novel green technology that shows potential approach for desalination along with electricity generation and wastewater treatment. However, the expensive catholyte/catalyst in the cathode side has limited the MDC for real time application. Hence, the main objective of this work was to investigate the electricity generation during dairy wastewater treatment and desalination efficiency using biocathode (Oscillatoria sp.) in the MDC. The results showed that the maximum open circuit voltage of 652 ± 10 mV, COD removal efficiency of 80.2 ± 0.5% and desalination efficiency of 65.8 ± 0.5%, were achieved respectively. The effect of saline water concentration was investigated and the performance of MDC was compared with real (sea) water. This study demonstrated that Oscillatoria sp. could be used as a potential biocatalyst in the cathode chamber for enhancing salinity removal along with electricity generation and wastewater treatment in the MDC.

Influence of Nickel molybdate nanocatalyst for enhancing biohydrogen production in microbial electrolysis cell utilizing sugar industrial effluent.

Biohydrogen production in Microbial Electrolysis Cell (MEC) had inspired the researchers to overcome the challenges associated towards sustainability. Despite microbial community and various substrates, economical cathode catalyst development is most significant factor for enhancing hydrogen production in the MEC. Hence, in this study, the performance of MEC was investigated with a sugar industry effluent (COD 4200 ± 20 mg/L) with graphite anode and modified Nickel foam (NF) cathode. Nickel molybdate (NiMoO4) coated NF achieved a higher hydrogen production rate 0.12 ± 0.01 L.L-1D-1 as compared to control under favorable conditions. Electrochemical characterizations demonstrated that the improved catalytic activity of novel nanocatalyst with lower impedance favoring faster hydrogen evolution kinetics. The MEC with the novel catalyst performed with 58.2% coloumbic efficiency, 20.36% cathodic hydrogen recovery, 11.96% overall hydrogen recovery and 54.38% COD removal efficiency for a 250 mL substrate during 5 days' batch cycle. Hence, the potentiality of modified cathode was established with the real time industrial effluent highlighting the waste to wealth bio-electrochemical technology.

"Sporotan" a new fluorescent stain for identifying cryptic spores of Rhodobacter johrii.

We propose a new fluorescent stain "sporotan" and staining protocol which aid in the identification of cryptic endospores which are otherwise mistaken as poly-ß-hydroxyalkanoate granules.

Zwitterionic small molecule based fluorophores for efficient and selective imaging of bacterial endospores.

In the emerging scenario of increasing antibiotic resistance and pathogen transmission channels, the grave danger posed by bacterial endospores in critical fields like food industry, health and medicine highlights the urgent need to develop efficient probes for their detection; their sturdy and impermeable multilayer coat makes desirable methods like fluorescence imaging extremely difficult. Selective imaging of the endospores in the presence of the bacteria is even more challenging. Furthermore, it is preferable to maintain the dormant state of endospores through the imaging process, if extended monitoring is required; many of the available techniques involve lethal germination or destruction of the endospores. We show that simple zwitterionic diaminodicyanoquinodimethane (DADQ) molecules with selected functionalities are efficient dyes for fluorescence imaging due to their dipolar structure that facilitates the penetration into the endospore system, and the enhanced fluorescence in their rigid/aggregated state. The facile structural tailorability allows DADQs with various appendage moieties to be synthesized; a derivative with ionic substituents (BT2), and another with optimally long alkyl chains and the resultant hydrophobic character (BHADQ) are shown to be excellent fluorescent probes for endospores. Nanomolar amounts of dyes provide effective staining; while BT2 stains bacteria and endospores, most significantly, BHADQ stains endospores selectively. To the best of our knowledge, this is the first example of selective fluorescence imaging of endospores in their dormant state. A range of spectroscopy, microscopy and calorimetry studies provide insight into the molecular level interactions that enable efficient staining and bright images. DADQ fluorophores are photostable and non-cytotoxic, hence useful in practical applications. The versatile structural tailorability of these dye molecules holds great promise for targeted imaging.

Steady expression of high oleic acid in peanut bred by marker-assisted backcrossing for fatty acid desaturase mutant alleles and its effect on seed germination along with other seedling traits.

Peanut (Arachis hypogaea L.) is an important nutrient-rich food legume and valued for its good quality cooking oil. The fatty acid content is the major determinant of the quality of the edible oil. The oils containing higher monounsaturated fatty acid are preferred for improved shelf life and potential health benefits. Therefore, a high oleic/linoleic fatty acid ratio is the target trait in an advanced breeding program. The two mutant alleles, ahFAD2A (on linkage group a09) and ahFAD2B (on linkage group b09) control fatty acid composition for higher oleic/linoleic ratio in peanut. In the present study, marker-assisted backcrossing was employed for the introgression of two FAD2 mutant alleles from SunOleic95R into the chromosome of ICGV06100, a high oil content peanut breeding line. In the marker-assisted backcrossing-introgression lines, a 97% increase in oleic acid, and a 92% reduction in linoleic acid content was observed in comparison to the recurrent parent. Besides, the oleic/linoleic ratio was increased to 25 with respect to the recurrent parent, which was only 1.2. The most significant outcome was the stable expression of oil-content, oleic acid, linoleic acid, and palmitic acid in the marker-assisted backcrossing-introgression lines over the locations. No significant difference was observed between high oleic and normal oleic in peanuts for seedling traits except germination percentage. In addition, marker-assisted backcrossing-introgression lines exhibited higher yield and resistance to foliar fungal diseases, i.e., late leaf spot and rust.

A Strongly Fluorescent Molecular Material Responsive to Physical/Chemical Stimuli and their Coupled Impact.

Molecular materials with weak but extended and pliable supramolecular interactions are versatile candidates for eliciting stimuli-sensitive optical responses. A novel diaminodicyanoquinodimethane (DADQ) molecule, 7,7-bis(2-(2-pyridyl)ethylamino)-8,8-dicyanoquinodimethane (BPEDQ), has been synthesized and structurally characterized; it exhibits enhanced fluorescence emission in the aggregated and solid states, characteristic of DADQs. The pyridine moieties in the molecule, in addition to the amino and cyano groups of the strongly dipolar fluorophore moiety, induce extensive H-bonding interactions which can impart structural integrity to the solid material; this enables reversible crystalline-amorphous transformations triggered by mechanical grinding and solvent fuming. The concomitant fluorescence color and intensity switching are prominent and reversible. Protonation-deprotonation events induced by acidic and basic vapors also produce stark fluorescence response variations; the chemical stimuli also lead to amorphization of the solid. The full cycle of chemical and physical stimuli, and the consequence of their individual and coupled impact on the fluorescence emission, are illustrated using a BPEDQ-doped polymer thin film.

Development of high oleic peanut lines through marker-assisted introgression of mutant ahFAD2 alleles and its fatty acid profiles under open-field and controlled conditions.

Peanut is one of the most important oilseed crops grown worldwide. In this study, the mutant ahFAD2 alleles conferring high oleic (HO) content are introgressed into an elite Indian cultivar GPBD4 which is also resistant to the foliar fungal diseases like rust and late leaf spot (LLS). The allele-specific PCR (AS-PCR) and cleaved amplified polymorphic sequences (CAPS) assays were used for the marker-assisted backcross (MABC) approach and 64 HO introgression lines (ILs) were generated. These ILs were tested for the FA compositions under the glasshouse and field conditions. The oleic acid and linoleic acid contents in the ILs were recorded to be between 68.94-82.33% and 1.74-10.87%, respectively, under glasshouse and 67.04-81.71% and 2.00-15.66%, respectively, under field conditions. The increase in the oleic acid content of the ILs over its recurrent parent (RP) was recorded to the tune of 28.78-53.80% and 33.70-62.96% under glasshouse and field conditions, respectively, indicating the stable expression of ahFAD2B gene in two different environments. On the contrary, linoleic acid showed 56.47-93.03% and 40.02-92.34% reduction in the ILs over its RP under glasshouse and field conditions, respectively. These ILs with a healthy FA profile can meet not only the nutritional requirements of a health-conscious society but also the industrial demands for better shelf life of oil and its products.

Designing a Low-Cost, Single-Supply ECG System for Suppression of Movement Artifact from Contaminated Magnetocardiogram.

Measurement of the late potentials and His-bundle activity is crucial for many clinical studies using the noncontact and noninvasive magnetocardiography (MCG) technique; these weak signals are extracted by averaging many cardiac cycles aligned using the R-peak of the cardiac cycle identified using an electrocardiography (ECG) lead. ECG is measured simultaneously with MCG using a conventional dual-supply ECG amplifier, which requires either two separate batteries or a single battery with a switching voltage inverter circuit for its proper operation. The ECG circuitry based on two separate batteries requires a relatively large voltage supply (-18 to +18 V). The single-supply (low voltage: 0-9 V) ECG circuitry may be implemented using a switching voltage inverter; however, this mode of operation introduces switching noise in the system. The objective of the present work is to overcome these problems by carefully designing a low-voltage, single-supply ECG system, which can be used simultaneously with the MCG setup without introducing a significant level of additional noise in the MCG measurement system.

Establishing the Critical Role of Oriented Aggregation in Molecular Solid State Fluorescence Enhancement.

The general occurrence of fluorescence emission quenching in molecular aggregates is circumvented in select classes of molecules. This has largely been attributed to the rigidification of the molecule and its environment, which hinders non-radiative excited state energy loss through structural relaxation; since such an effect should in principle apply to most aggregates and crystals, there must clearly be other critical factors that make the select molecules exceptional. Discovery of three crystalline structures of a new push-pull molecule in its enantiomorphic and racemic forms, exhibiting not only very high, but distinctly different solid state fluorescence enhancements, has now allowed a systematic investigation of the role of intramolecular and intermolecular excited state energy loss pathways. Crystallographic, spectroscopic and computational investigations provide a detailed appraisal of the assembly patterns in the crystals, and rigorous establishment of an inverse correlation between intermolecular energy transfer and solid state fluorescence. The study provides a clear visualization of the critical role of oriented molecular aggregation in solid state fluorescence efficiency enhancement.

Optimal controller synthesis for second order time delay systems with at least one RHP pole.

An optimal H2 minimization framework is proposed in this paper for devising a controller of PID in nature, based on a refined IMC filter configuration. The tuning strategy is for controlling time delay system with at least one pole which falls on the right half of the s-plane. An underdamped model based filter is used in place of the unity damping ratio (critically damped) filter available in the literature to improve the reset action. The method has a single adjustable closed loop tuning parameter. Guidelines have been provided for choosing the pertinent tuning parameter based on the sensitivity function. Simulation work has been executed on diverse unstable models to support the advantages of the proposed scheme. The proposed controller yields improved performances over other recently reported tuning techniques in the literature. Experimental implementation is carried out on an inverted pendulum for demonstrating the practical applicability of the present method. The efficacy of the intended controller design is quantitatively analyzed using the time integral performance index.

Gold nanoclusters with a wide range of fluorescence characteristics generated in situ in polymer thin films: potential gas sensing application.

Fabrication of gold nanoclusters (GNCs) with tunable fluorescence characteristics inside polymer thin films is attractive from the device application perspective. In this study, GNCs are generated in situ in poly(methyl methacrylate) films exploiting their weak reducing capability with no additional reducing agent, and by short and mild thermal annealing; the chemistry involved is probed through control experiments. The nanoclusters formed with ∼0.5 weight percent of gold are very stable and show appreciable fluorescence emission with a small Stokes shift (∼40 nm); interestingly, blending polystyrene enhances the fluorescence. The Au10 clusters formed in situ are characterized by using mass spectrometry, microscopy and computational modeling. Composite thin films fabricated with a gold content of ∼9 weight percent showed an unusually distinct absorption peak and enhanced fluorescence emission. Gradual coalescence of the nanoclusters in these films could be arrested by incorporating thiourea; the mechanistic aspects of the thiourea interaction are probed. The resulting films showed strong, stable and visible red emission, with very large Stokes shift (∼320 nm) and quantum yield (∼30%), attributable to ligand effects and nanocluster aggregation in the film. The study presents a novel and facile route to the in situ generation of GNCs in polymer thin films, exhibiting fluorescence emission with variable energy, intensity and Stokes shift. Preliminary experiments show that Au10 cluster embedded thin films can be used for the detection of POCl3, an important precursor for nerve agents.

Efficient Bioimaging with Diaminodicyanoquinodimethanes: Selective Imaging of Epidermal and Stomatal Cells and Insight into the Molecular Level Interactions.

The enhanced fluorescence emission of diaminodicyanoquinodimethanes (DADQs) in rigid and aggregated states holds great promise for bioimaging applications. This is demonstrated through their efficient application in epidermal and stomatal imaging with selective staining of cell walls and nuclei. Major advantages include the small quantities (a few nmols) of the fluorophore required, choice of DADQs soluble in water and organic solvents, and quick staining of the specimen in buffer-free state and in buffer medium. The molecular level interactions that enable staining are unraveled through isothermal calorimetry, infra-red spectroscopy and microscopy with energy dispersive X-ray spectroscopy analysis. It is proposed that DADQs with ionic or H-bonding functionalities bind to the polygalacturonic acid moieties in the epidermal layer; the former can bind also to nucleic acid polyanions. Fluorescence experiments explain the emission enhancement that enables the efficient imaging. DADQs are easy to synthesize, non-cytotoxic, and thermally, chemically and photo-stable, requiring no special storage conditions; preliminary experiments point to their potential utility in imaging different classes of cells.

Soft Chemical Fabrication of Iron-Based Thin Film Electrocatalyst for Water Oxidation under Neutral pH and Structure-Activity Tuning by Cerium Incorporation.

Design of electrocatalysts for the fundamentally important oxygen evolution reaction can be greatly aided by systematic structure-activity tuning via composition variation. We have explored the iron-cerium system as they are the most abundant transition and rare earth metals, and also due to the mutualistic impact of their size and electronic attributes that can induce critical changes in the structure and electrochemical activity. Submicrometer thick films of a series of Fe(III)-Ce(III) phosphate(oxyhydroxide) (FeCePH) are fabricated using a soft chemical strategy involving surfactant-aided assembly, spin-coating, and mild thermal annealing. FT-IR, Raman, and X-ray photoelectron spectroscopies, chemical analysis, X-ray diffraction, and electron microscopy reveal the systematic structural, electronic, and morphological variation, on tuning the iron-cerium composition. Nitrogen adsorption-desorption studies show the surface area increasing and pore size distribution shrinking with the cerium content, indicating its structure-directing role. The electrocatalysis of water oxidation by FeCePH films on FTO-coated glass is studied in neutral pH conditions. The overpotential and Tafel slope decrease with increasing cerium content, reaching minima at the optimal Fe:Ce ratio of 1:0.5; the turnover frequency shows a corresponding increase and maximum. The trends are explained on the basis of the structural changes in the films, and the coupling of Ce3+/Ce4+ with Fe3+/Fe4+ that leads to active state regeneration. This study presents a rational strategy to tune the efficiency of easily fabricated transition metal-based electrocatalyst thin films through rare earth metal incorporation; it should prove useful in the design of cost-effective catalysts for water oxidation.

Enhanced IMC based PID controller design for non-minimum phase (NMP) integrating processes with time delays.

Internal model control (IMC) with optimal H2 minimization framework is proposed in this paper for design of proportional-integral-derivative (PID) controllers. The controller design is addressed for integrating and double integrating time delay processes with right half plane (RHP) zeros. Blaschke product is used to derive the optimal controller. There is a single adjustable closed loop tuning parameter for controller design. Systematic guidelines are provided for selection of this tuning parameter based on maximum sensitivity. Simulation studies have been carried out on various integrating time delay processes to show the advantages of the proposed method. The proposed controller provides enhanced closed loop performances when compared to recently reported methods in the literature. Quantitative comparative analysis has been carried out using the performance indices, Integral Absolute Error (IAE) and Total Variation (TV).

Common Methodology for Cardiac and Ocular Artifact Suppression from EEG Recordings by Combining Ensemble Empirical Mode Decomposition with Regression Approach.

Electroencephalography (EEG) is a non-invasive way of recording brain activities, making it useful for diagnosing various neurological disorders. However, artifact signals associated with eye blinks or the heart spread across the scalp, contaminating EEG recordings and making EEG data analysis difficult. To solve this problem, we implement a common methodology to suppress both cardiac and ocular artifact signal, by correlating the measured contaminated EEG signals with the clean reference electro-oculography (EOG) and electrocardiography (EKG) data and subtracting the scaled EOG and EKG from the contaminated EEG recording. In the proposed methodology, the clean EOG and EKG signals are extracted by subjecting the raw reference time-series data to ensemble empirical mode decomposition to obtain the intrinsic mode functions. Then, an unsupervised technique is used to capture the artifact components. We compare the distortion introduced into the brain signal after artifact suppression using the proposed method with those obtained using conventional regression alone and with a wavelet-based approach. The results show that the proposed method outperforms the other techniques, with an additional advantage of being a common methodology for the suppression of two types of artifact.

Genomic Tools in Groundnut Breeding Program: Status and Perspectives.

Groundnut, a nutrient-rich food legume, is cultivated world over. It is valued for its good quality cooking oil, energy and protein rich food, and nutrient-rich fodder. Globally, groundnut improvement programs have developed varieties to meet the preferences of farmers, traders, processors, and consumers. Enhanced yield, tolerance to biotic and abiotic stresses and quality parameters have been the target traits. Spurt in genetic information of groundnut was facilitated by development of molecular markers, genetic, and physical maps, generation of expressed sequence tags (EST), discovery of genes, and identification of quantitative trait loci (QTL) for some important biotic and abiotic stresses and quality traits. The first groundnut variety developed using marker assisted breeding (MAB) was registered in 2003. Since then, USA, China, Japan, and India have begun to use genomic tools in routine groundnut improvement programs. Introgression lines that combine foliar fungal disease resistance and early maturity were developed using MAB. Establishment of marker-trait associations (MTA) paved way to integrate genomic tools in groundnut breeding for accelerated genetic gain. Genomic Selection (GS) tools are employed to improve drought tolerance and pod yield, governed by several minor effect QTLs. Draft genome sequence and low cost genotyping tools such as genotyping by sequencing (GBS) are expected to accelerate use of genomic tools to enhance genetic gains for target traits in groundnut.

Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes.

High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 (ahFAD2A) and b09 (ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2 mutant alleles from SunOleic 95R into the genetic background of ICGV 06110, ICGV 06142 and ICGV 06420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5-1.1 folds, with concomitant reduction of linoleic acid by 0.4-1.0 folds and palmitic acid by 0.1-0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53-58%), and 28 ILs with low oil content (42-50%) were selected that may be released for cultivation upon further evaluation.