Analysis of follicular fluid total phospholipids in women undergoing in-vitro fertilisation.

Follicular fluid (FF) samples were obtained from 100 patients referred to the University Hospital. A total of 79 subjects underwent IVF and the remaining 21 underwent ICSI. The levels of apoA-I and total phospholipid were measured using turbidometric and colorimetric phosphorus assays, respectively. Correlation analysis showed a significant inverse association of total phospholipid in FF with fertilisation ratio (r = -0.24, p = 0.04). Furthermore, the ratio of phospholipid/apoA-I in patients with a percentage of fertilised oocytes ≤ 50% was significantly higher (> 2.5%, p < 0.05) than in those with higher percentages of fertilised oocytes. The amounts of phospholipid and phospholipid/apoA-I ratio in FF were associated negatively to the percentage of oocyte fertilisation. Therefore, the change in the phospholipid and phospholipid/apoA-I ratio of FF might be regarded as indicators of female fertility.

Cholesteryl ester transfer protein I405V polymorphism influences apolipoprotein A-I response to a change in dietary fatty acid composition.

The aim of the present study was to investigate whether the cholesteryl ester transfer protein (CETP) I405V polymorphism modifies the response to changes in the dietary ratio of polyunsaturated to saturated fat (P:S). The population included 85 healthy subjects with the different I405V genotypes (35 II, 36 IV, and 14 VV) assigned to two consecutive 28-day experimental period. All subjects consumed a high-P:S with P:S of 1.2 for the first period and a low-P:S with a P:S of 0.3 for the next 28-day period. At the first and end of each dietary period, serum lipid, lipoprotein, and CETP concentrations were measured. At screening, lipid or lipoprotein concentrations were not significantly different among CETP I405V genotype groups. After the low-P:S diet, subjects carrying V allele had greater reduction in apoA-I and HDL cholesterol (HDL-C) than subjects with II genotype. A genotype-by-diet interaction effect was observed on apoA-I (p=0.016) concentrations. In conclusion, the CETP I405V polymorphism contributes to the unfavorable changes of apoA-I and HDL-C when a high-P:S diet was replaced with a low-P:S diet.