RESUMO
Rabies is endemic throughout most of Asia, with the majority of human cases transmitted by domestic dogs (Canis familiaris). Here, we report a case of rabies in a 12-year-old girl in the Lalitpur district of Nepal that might have been prevented by better public awareness and timely post-exposure prophylaxis. Molecular characterization of the virus showed 100% identity over a partial nucleoprotein gene sequence to previous isolates from Nepal belonging to the 'arctic-like' lineage of rabies virus. Sequence analysis of both partial nucleoprotein and glycoprotein genes showed differences in consensus sequence after passage in vitro but not after passage in vivo.
Assuntos
RNA Viral/genética , Vírus da Raiva/genética , Vírus da Raiva/isolamento & purificação , Raiva/virologia , Criança , Análise por Conglomerados , Feminino , Humanos , Dados de Sequência Molecular , Nepal , Filogenia , Vírus da Raiva/classificação , Análise de Sequência de DNA , Homologia de Sequência , Proteínas Virais/genéticaRESUMO
In 1987 and 1990, serum samples were collected from people living in the two districts (Itahari and Chitwan) of the Terai region of Nepal. Antibodies against Japanese encephalitis (JE) virus in these sera were detected by the hemagglutination inhibition (HI) and neutralization (N) tests. By the HI test, 26 out of 172 (15.1%) sera from Chitwan and 15 out of 137 (10.9%) sera from Itahari showed positive titers. Higher positive rates were shown by the N test, where 46 out of 172 (26.7%) sera from Chitwan and 22 out of 137 (16.1%) sera from Itahari had antibodies against JE virus. A JE strain was isolated from a blood specimen of a pig raised in Kathmandu. When the nucleotide sequence of the pre-M region of the strain was compared to the same region of the other JE virus strains reported, the highest similarity was observed to the strains isolated in Nepal in 1985. These results suggest that the Terai region has been an epidemic area of JE.
Assuntos
DNA , Encefalite Japonesa/epidemiologia , RNA , Adolescente , Adulto , Idoso , Sequência de Bases , Criança , Pré-Escolar , Encefalite Japonesa/genética , Encefalite Japonesa/microbiologia , Feminino , Testes de Inibição da Hemaglutinação/normas , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Nepal/epidemiologia , Testes de Neutralização/normas , Reação em Cadeia da Polimerase , Sensibilidade e Especificidade , Estudos Soroepidemiológicos , SorotipagemRESUMO
Inoculations with isolates of Macrophomina phaseolina and Sclerotinia sclerotiorum resulted in a significant increase in the acid phosphatase activity of susceptible and resistant cultivars of Brassica juncea, which appeared to be related to the disease reaction of different host-pathogen combinations. After an increase on the 6th day of inoculation there was usually a fall in activity on the 12th and 18th days. Resistant cultivars showed very poor activity in comparison to their susceptible counterparts.
