RESUMO
Learning requires the ability to link actions to outcomes. How motivation facilitates learning is not well understood. We designed a behavioral task in which mice self-initiate trials to learn cue-reward contingencies and found that the anterior cingulate region of the prefrontal cortex (ACC) contains motivation-related signals to maximize rewards. In particular, we found that ACC neural activity was consistently tied to trial initiations where mice seek to leave unrewarded cues to reach reward-associated cues. Notably, this neural signal persisted over consecutive unrewarded cues until reward-associated cues were reached, and was required for learning. To determine how ACC inherits this motivational signal we performed projection-specific photometry recordings from several inputs to ACC during learning. In doing so, we identified a ramp in bulk neural activity in orbitofrontal cortex (OFC)-to-ACC projections as mice received unrewarded cues, which continued ramping across consecutive unrewarded cues, and finally peaked upon reaching a reward-associated cue, thus maintaining an extended motivational state. Cellular resolution imaging of OFC confirmed these neural correlates of motivation, and further delineated separate ensembles of neurons that sequentially tiled the ramp. Together, these results identify a mechanism by which OFC maps out task structure to convey an extended motivational state to ACC to facilitate goal-directed learning.
Achieving goals takes motivation. An individual may have to complete a task many times for a future reward. For example, an animal may have to forage repeatedly to find food, or a person may have to study to get a good grade on a test. How these complex behaviors are encoded in the brain's wiring is not fully understood. Patients with injuries to the frontal cortex of the brain display a lack of motivation to pursue goals. This discovery suggests the frontal cortex plays a vital role in motivation and goal-directed behavior. Animal studies show that part of their brain's frontal cortex, the anterior cingulate cortex (ACC), helps them stay motivated and put extra effort into achieving goals. Yet, scientists wonder how particular actions are associated with specific goals and suspect the orbital frontal cortex (OFC) contains the blueprint to support this association. Regalado et al. show that the OFC and ACC work together during goal-seeking behavior in mice. In the experiments, mice learned to complete a task to achieve a sugar water reward. As the mice were learning, Regalado et al. recorded activity in the ACC and found that the ACC is active during goal-seeking behavior. They also discovered that the activity of neurons in the OFC increased the longer mice went without receiving a reward, up until the reward was achieved, signaling a motivational state. Animals not motivated enough to maximize their rewards did not have an increased OFC activity. The experiments also showed that the motivational signals in the OFC were conveyed to ACC to support goal-directed learning, especially linking actions to positive future outcomes. The experiments help explain how an increase in neuronal activity in the OFC helps to increase motivation and goal-seeking behavior supported by the ACC. More studies will help scientists learn more about these processes and develop drugs or other therapies that can help people who have learning difficulties or struggle with motivation because of an injury or mental illness.
Assuntos
Aprendizagem , Motivação , Córtex Pré-Frontal , Recompensa , Animais , Motivação/fisiologia , Camundongos , Aprendizagem/fisiologia , Córtex Pré-Frontal/fisiologia , Sinais (Psicologia) , Neurônios/fisiologia , Masculino , Giro do Cíngulo/fisiologia , Camundongos Endogâmicos C57BL , Comportamento Animal/fisiologiaRESUMO
Memory formation involves binding of contextual features into a unitary representation1-4, whereas memory recall can occur using partial combinations of these contextual features. The neural basis underlying the relationship between a contextual memory and its constituent features is not well understood; in particular, where features are represented in the brain and how they drive recall. Here, to gain insight into this question, we developed a behavioural task in which mice use features to recall an associated contextual memory. We performed longitudinal imaging in hippocampus as mice performed this task and identified robust representations of global context but not of individual features. To identify putative brain regions that provide feature inputs to hippocampus, we inhibited cortical afferents while imaging hippocampus during behaviour. We found that whereas inhibition of entorhinal cortex led to broad silencing of hippocampus, inhibition of prefrontal anterior cingulate led to a highly specific silencing of context neurons and deficits in feature-based recall. We next developed a preparation for simultaneous imaging of anterior cingulate and hippocampus during behaviour, which revealed robust population-level representation of features in anterior cingulate, that lag hippocampus context representations during training but dynamically reorganize to lead and target recruitment of context ensembles in hippocampus during recall. Together, we provide the first mechanistic insights into where contextual features are represented in the brain, how they emerge, and how they access long-range episodic representations to drive memory recall.
Assuntos
Giro do Cíngulo , Hipocampo , Rememoração Mental , Modelos Neurológicos , Animais , Mapeamento Encefálico , Córtex Entorrinal/citologia , Córtex Entorrinal/fisiologia , Giro do Cíngulo/citologia , Giro do Cíngulo/fisiologia , Hipocampo/citologia , Hipocampo/fisiologia , Estudos Longitudinais , Rememoração Mental/fisiologia , Camundongos , Inibição NeuralRESUMO
Working memory is a form of short-term memory that involves maintaining and updating task-relevant information toward goal-directed pursuits. Classical models posit persistent activity in prefrontal cortex (PFC) as a primary neural correlate, but emerging views suggest additional mechanisms may exist. We screened â¼200 genetically diverse mice on a working memory task and identified a genetic locus on chromosome 5 that contributes to a substantial proportion (17%) of the phenotypic variance. Within the locus, we identified a gene encoding an orphan G-protein-coupled receptor, Gpr12, which is sufficient to drive substantial and bidirectional changes in working memory. Molecular, cellular, and imaging studies revealed that Gpr12 enables high thalamus-PFC synchrony to support memory maintenance and choice accuracy. These findings identify an orphan receptor as a potent modifier of short-term memory and supplement classical PFC-based models with an emerging thalamus-centric framework for the mechanistic understanding of working memory.
Assuntos
Memória de Curto Prazo/fisiologia , Receptores Acoplados a Proteínas G/genética , Tálamo/metabolismo , Animais , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Vias Neurais/fisiologia , Neurônios/metabolismo , Neurônios/fisiologia , Córtex Pré-Frontal/fisiologia , Receptores Acoplados a Proteínas G/metabolismoRESUMO
Piwi-interacting RNAs (piRNAs), long thought to be restricted to germline, have recently been discovered in neurons of Aplysia, with a role in the epigenetic regulation of gene expression underlying long-term memory. We here ask whether piwi/piRNAs are also expressed and have functional roles in the mammalian brain. Large-scale RNA sequencing and subsequent analysis of protein expression revealed the presence in brain of several piRNA biogenesis factors including a mouse piwi (Mili), as well as small RNAs, albeit at low levels, resembling conserved piRNAs in mouse testes [primarily LINE1 (long interspersed nuclear element1) retrotransposon-derived]. Despite the seeming low expression of these putative piRNAs, single-base pair CpG methylation analyses across the genome of Mili/piRNA-deficient (Mili-/- ) mice demonstrate that brain genomic DNA is preferentially hypomethylated within intergenic areas and LINE1 promoter areas of the genome. Furthermore, Mili mutant mice exhibit behavioral deficits such as hyperactivity and reduced anxiety. These results suggest that putative piRNAs exist in mammalian brain, and similar to the role of piRNAs in testes, they may be involved in the silencing of retrotransposons, which in brain have critical roles in contributing to genomic heterogeneity underlying adaptation, stress response, and brain pathology. We also describe the presence of another class of small RNAs in the brain, with features of endogenous siRNAs, which may have taken over the role of invertebrate piRNAs in their capacity to target both transposons, as well as protein-coding genes. Thus, RNA interference through gene and retrotransposon silencing previously encountered in Aplysia may also have potential roles in the mammalian brain.
RESUMO
Optogenetic methodology enables direct targeting of specific neural circuit elements for inhibition or excitation while spanning timescales from the acute (milliseconds) to the chronic (many days or more). Although the impact of this temporal versatility and cellular specificity has been greater for basic science than clinical research, it is natural to ask whether the dynamic patterns of neural circuit activity discovered to be causal in adaptive or maladaptive behaviors could become targets for treatment of neuropsychiatric diseases. Here, we consider the landscape of ideas related to therapeutic targeting of circuit dynamics. Specifically, we highlight optical, ultrasonic, and magnetic concepts for the targeted control of neural activity, preclinical/clinical discovery opportunities, and recently reported optogenetically guided clinical outcomes.
Assuntos
Encéfalo/fisiologia , Vias Neurais , Optogenética/métodos , Animais , Encéfalo/citologia , Fenômenos Eletromagnéticos , Humanos , Neurônios/fisiologia , Opsinas/fisiologiaRESUMO
In recently developed approaches for high-resolution imaging within intact tissue, molecular characterization over large volumes has been largely restricted to labeling of proteins. But volumetric nucleic acid labeling may represent a far greater scientific and clinical opportunity, enabling detection of not only diverse coding RNA variants but also non-coding RNAs. Moreover, scaling immunohistochemical detection to large tissue volumes has limitations due to high cost, limited renewability/availability, and restricted multiplexing capability of antibody labels. With the goal of versatile, high-content, and scalable molecular phenotyping of intact tissues, we developed a method using carbodiimide-based chemistry to stably retain RNAs in clarified tissue, coupled with amplification tools for multiplexed detection. The resulting technology enables robust measurement of activity-dependent transcriptional signatures, cell-identity markers, and diverse non-coding RNAs in rodent and human tissue volumes. The growing set of validated probes is deposited in an online resource for nucleating related developments from across the scientific community.
Assuntos
Química Encefálica , Hibridização In Situ/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , RNA/análise , Transcriptoma , Adolescente , Animais , Cianatos/química , Etildimetilaminopropil Carbodi-Imida/química , Feminino , Humanos , Masculino , Maleimidas/química , Camundongos , Pessoa de Meia-Idade , Oligonucleotídeos/química , Succinimidas/químicaRESUMO
Top-down prefrontal cortex inputs to the hippocampus have been hypothesized to be important in memory consolidation, retrieval, and the pathophysiology of major psychiatric diseases; however, no such direct projections have been identified and functionally described. Here we report the discovery of a monosynaptic prefrontal cortex (predominantly anterior cingulate) to hippocampus (CA3 to CA1 region) projection in mice, and find that optogenetic manipulation of this projection (here termed AC-CA) is capable of eliciting contextual memory retrieval. To explore the network mechanisms of this process, we developed and applied tools to observe cellular-resolution neural activity in the hippocampus while stimulating AC-CA projections during memory retrieval in mice behaving in virtual-reality environments. Using this approach, we found that learning drives the emergence of a sparse class of neurons in CA2/CA3 that are highly correlated with the local network and that lead synchronous population activity events; these neurons are then preferentially recruited by the AC-CA projection during memory retrieval. These findings reveal a sparsely implemented memory retrieval mechanism in the hippocampus that operates via direct top-down prefrontal input, with implications for the patterning and storage of salient memory representations.
Assuntos
Memória/fisiologia , Neocórtex/citologia , Neocórtex/fisiologia , Vias Neurais/fisiologia , Animais , Condicionamento Psicológico , Medo , Giro do Cíngulo/fisiologia , Hipocampo/citologia , Hipocampo/fisiologia , Aprendizagem/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Modelos Neurológicos , Neurônios/fisiologia , Optogenética , Córtex Pré-Frontal/fisiologia , Interface Usuário-ComputadorRESUMO
The maintenance phase of memory-related long-term facilitation (LTF) of synapses between sensory and motor neurons of the gill-withdrawal reflex of Aplysia depends on a serotonin (5-HT)-triggered presynaptic upregulation of CPEB, a functional prion that regulates local protein synthesis at the synapse. The mechanisms whereby serotonin regulates CPEB levels in presynaptic sensory neurons are not known. Here, we describe a sensory neuron-specific microRNA 22 (miR-22) that has multiple binding sites on the mRNA of CPEB and inhibits it in the basal state. Serotonin triggers MAPK/Erk-dependent downregulation of miR-22, thereby upregulating the expression of CPEB, which in turn regulates, through functional CPE elements, the presynaptic expression of atypical PKC (aPKC), another candidate regulator of memory maintenance. Our findings support a model in which the neurotransmitter-triggered downregulation of miR-22 coordinates the regulation of genes contributing synergistically to the long-term maintenance of memory-related synaptic plasticity.
Assuntos
Aplysia/fisiologia , MicroRNAs/metabolismo , Proteínas de Ligação a RNA/metabolismo , Serotonina/metabolismo , Animais , Sítios de Ligação , Potenciação de Longa Duração/genética , Memória/fisiologia , MicroRNAs/genética , Neurônios Motores/metabolismo , Neurônios Motores/fisiologia , Plasticidade Neuronal/genética , Biossíntese de Proteínas , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/genética , Células Receptoras Sensoriais , Serotonina/genética , Sinapses/genética , Sinapses/metabolismoRESUMO
In recent years, a greater understanding has emerged of the role epigenetic mechanisms play in the brain, not only during development, but also in mature neurons involved in long-term memory. The identification of spatially and temporally tuned epigenetic modification of genetic loci during memory storage has revealed the remarkably input-responsive, target-specific, and long-term nature of epigenetic regulation, but the underlying mechanisms have remained elusive. New insight into these mechanisms has come from the study of small RNAs, which have emerged as regulators that can confer sequence specificity to DNA- and chromatin-modifying processes. We discuss advances in the elucidation of the epigenetic mechanisms involved in long-term memory, focusing on the role of small RNAs, and in particular piwi-interacting RNAs (piRNAs), in the epigenetic regulation underlying memory storage.
Assuntos
Epigenômica , Memória/fisiologia , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , Animais , HumanosRESUMO
Here we describe a strategy designed to identify RNAs that are actively transported to synapses during learning. Our approach is based on the characterization of RNA transport complexes carried by molecular motor kinesin. Using this strategy in Aplysia, we have identified 5,657 unique sequences consisting of both coding and noncoding RNAs from the CNS. Several of these RNAs have key roles in the maintenance of synaptic function and growth. One of these RNAs, myosin heavy chain, is critical in presynaptic sensory neurons for the establishment of long-term facilitation, but not for its persistence.
Assuntos
Aplysia/genética , Perfilação da Expressão Gênica/métodos , Sinapses/genética , Transcriptoma/genética , Animais , Sistema Nervoso Central/metabolismo , Genoma/genética , Sequenciamento de Nucleotídeos em Larga Escala , Hibridização In Situ , Cinesinas/metabolismo , Potenciação de Longa Duração/genética , Cadeias Pesadas de Miosina/metabolismo , Neurônios/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Transporte Proteico/genética , RNA/genética , RNA/metabolismo , Transporte de RNA/genética , Análise de Sequência de RNARESUMO
Small RNA-mediated gene regulation during development causes long-lasting changes in cellular phenotypes. To determine whether small RNAs of the adult brain can regulate memory storage, a process that requires stable and long-lasting changes in the functional state of neurons, we generated small RNA libraries from the Aplysia CNS. In these libraries, we discovered an unexpectedly abundant expression of a 28 nucleotide sized class of piRNAs in brain, which had been thought to be germline specific. These piRNAs have unique biogenesis patterns, predominant nuclear localization, and robust sensitivity to serotonin, a modulatory transmitter that is important for memory. We find that the Piwi/piRNA complex facilitates serotonin-dependent methylation of a conserved CpG island in the promoter of CREB2, the major inhibitory constraint of memory in Aplysia, leading to enhanced long-term synaptic facilitation. These findings provide a small RNA-mediated gene regulatory mechanism for establishing stable long-term changes in neurons for the persistence of memory.
Assuntos
Epigenômica , Memória , Plasticidade Neuronal , Neurônios/fisiologia , RNA Interferente Pequeno/metabolismo , Animais , Aplysia/metabolismo , Sequência de Bases , Regulação da Expressão Gênica , Humanos , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/metabolismo , Sinapses/metabolismoRESUMO
Memory storage and memory-related synaptic plasticity rely on precise spatiotemporal regulation of gene expression. To explore the role of small regulatory RNAs in learning-related synaptic plasticity, we carried out massive parallel sequencing to profile the small RNAs of Aplysia californica. We identified 170 distinct miRNAs, 13 of which were novel and specific to Aplysia. Nine miRNAs were brain enriched, and several of these were rapidly downregulated by transient exposure to serotonin, a modulatory neurotransmitter released during learning. Further characterization of the brain-enriched miRNAs revealed that miR-124, the most abundant and well-conserved brain-specific miRNA, was exclusively present presynaptically in a sensory-motor synapse where it constrains serotonin-induced synaptic facilitation through regulation of the transcriptional factor CREB. We therefore present direct evidence that a modulatory neurotransmitter important for learning can regulate the levels of small RNAs and present a role for miR-124 in long-term plasticity of synapses in the mature nervous system.
Assuntos
Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , MicroRNAs/fisiologia , Plasticidade Neuronal/fisiologia , Sinapses/fisiologia , Análise de Variância , Animais , Aplysia/fisiologia , Sistema Nervoso Central/citologia , Sistema Nervoso Central/efeitos dos fármacos , Sistema Nervoso Central/fisiologia , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Evolução Molecular , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/genética , Cinesinas/metabolismo , MicroRNAs/antagonistas & inibidores , Biologia Molecular/métodos , Mutação/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/genética , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Oligonucleotídeos Antissenso/farmacologia , Ligação Proteica/efeitos dos fármacos , Serotonina/farmacologia , Sinapses/efeitos dos fármacos , Fatores de Tempo , Ubiquitina Tiolesterase/metabolismoRESUMO
Systems modeling is emerging as a valuable tool in therapeutics. This is seen by the increasing use of clinically relevant computational models and a rise in systems biology companies working with the pharmaceutical industry. Systems models have helped understand the effects of pharmacological intervention at receptor, intracellular and intercellular communication stages of cell signaling. For instance, angiogenesis models at the ligand-receptor interaction level have suggested explanations for the failure of therapies for cardiovascular disease. Intracellular models of myeloma signaling have been used to explore alternative drug targets and treatment schedules. Finally, modeling has suggested novel approaches to treating disorders of intercellular communication, such as diabetes. Systems modeling can thus fill an important niche in therapeutics by making drug discovery a faster and more systematic process.
Assuntos
Desenho de Fármacos , Avaliação Pré-Clínica de Medicamentos/métodos , Teoria de Sistemas , Animais , Humanos , Transdução de Sinais/efeitos dos fármacosRESUMO
The gamma-aminobutyric acid (GABA(A)) receptor belongs to a superfamily of membrane-bound proteins that regulate signal transmission between cells in the nervous system. It is the target of convulsants such as picrotoxin and is mutated in some forms of epilepsy, a disease affecting approximately 50 million people worldwide. In picrotoxin inhibition and in one form of epilepsy, a decrease in the channel-opening equilibrium of a GABA(A) receptor is responsible for receptor dysfunction. Here we identify compounds that can regulate the channel-opening equilibrium of the GABA(A) receptor. Fluorinated RNA polymers containing a 40-nucleotide region with a randomized sequence were used to select those that can displace picrotoxin from the membrane-bound GABA(A) receptor in the rat forebrain. After 11 selection rounds, two classes of RNA molecules that bind to the GABA(A) receptor with nanomolar affinity were isolated and sequenced. Class I and class II molecules have different consensus sequences and different binding affinities for the receptor. A transient kinetic technique, the cell-flow method, was employed in combination with the whole-cell current-recording technique to determine the affinity of the selected RNA aptamers for the GABA(A) receptor. Class I molecules have a higher affinity for the closed-channel form than for the open-channel receptor form and inhibit the receptor; class II aptamers bind with equal or higher affinity to the open-channel form and alleviate picrotoxin inhibition.