Enhanced Osteogenic Potential of Phosphonated-Siloxane Hydrogel Scaffolds.

In a material-guided approach, instructive scaffolds that leverage potent chemistries may efficiently promote bone regeneration. A siloxane macromer has been previously shown to impart osteoinductivity and bioactivity when included in poly(ethylene glycol) diacrylate (PEG-DA) hydrogel scaffolds. Herein, phosphonated-siloxane macromers were evaluated for enhancing the osteogenic potential of siloxane-containing PEG-DA scaffolds. Two macromers were prepared with different phosphonate pendant group concentrations, poly(diethyl(2-(propylthio)ethyl)phosphonate methylsiloxane) diacrylate (PPMS-DA) and 25%-phosphonated analogue (PPMS-DA 25%). Macroporous, templated scaffolds were prepared by cross-linking these macromers with PEG-DA at varying mol % (15:85, 30:70, and 45:55 PPMS-DA to PEG-DA; 30:70 PPMS-DA 25% to PEG-DA). Other scaffolds were also prepared by combining PEG-DA with PDMS-MA (i.e., no phosphonate) or with vinyl phosphonate (i.e., no siloxane). Scaffold material properties were thoroughly assessed, including pore morphology, hydrophobicity, swelling, modulus, and bioactivity. Scaffolds were cultured with human bone marrow-derived mesenchymal stem cells (normal media) and calcium deposition and protein expression were assessed at 14 and 28 days.

Spatially Controlled Templated Hydrogels for Orthopedic Interfacial Tissue Regeneration.

Scaffolds that recapitulate the spatial complexity of orthopedic interfacial tissues are essential to their regeneration. This requires a method to readily and flexibly produce scaffolds with spatial control over physical and chemical properties, without resulting in hard interfaces. Herein, we produced hydrogel scaffolds with spatially tunable arrangements and chemistries (SSTACs). Using solvent-induced phase separation/fused salt templating (SIPS/salt), scaffold elements are initially prepared with a tunable pore size and with one or more UV-reactive macromers. After trimming to the desired dimensions, these are physically configured and fused together to form the SSTACs. Using this method, three SSTAC designs were prepared, including one that mimicked the osteochondral interface. Bright-field/fluorescent microscopy revealed spatial control of pore size and chemical composition across a relatively smooth and integrated interface, regardless of layer composition. An interface formed by a SSTAC was determined to withstand a similar shear force to an analogous scaffold with no interface.

[Influence of health centers and social class in the prevalence of breast feeding]. / Influencia de los centros de salud y la clase social en la prevalencia de la lactancia materna.

AIMS: To find out the prevalence of breastfeeding in the Health District of Córdoba, taking into account the basic health areas it is composed of; to find out if breastfeeding is less frequent in lower social classes than in high ones and the influence of health centers on breastfeeding. DESIGN: Cross-sectional descriptive study. Home survey. SITE: Health District of Córdoba, urban area. SUBJECTS: 1, 3, 6 months old babies taken from the computerized newborn register of the Health District of Córdoba. MEASUREMENTS AND RESULTS: Age of the babies; breastfeeding, social class, primary care model (basic health areas attended by Health Centers or not attended by Health Centers), weight at birth, parents' jobs, pregnancy control, maternal education and number of visits. 77.2% of the mothers start breastfeeding. 51.7% of one month-old babies are breastfed. One out of four three-month-old babies are breastfed and one out of ten six-month-old babies are breastfed. Low weight at birth has a negative influence on breastfeeding. The prevalence of breastfeeding is higher in favoured classes, and in the basic health areas attended by health centers. The rest of variables analyzed are not significant. CONCLUSIONS: The health centers improve the prevalence and duration of breastfeeding.