Inhibition of enteropathogenic Escherichia coli adhesion to HeLa cells by serum of infants with diarrhea and by cord serum

We have studied the effect of serum from infants with diarrhea and of cord serum on the localized adherence of enteropathogenic Escherichia coli (EPEC) to HeLa cells. Serum samples from 16 infants with diarrhea due to EPEC of serotypes O55:H6, O111: H-, O111:H2, O119:H6 and O142:H6 were used. The adherence ability of EPEC strains belonging to serotypes identical to (homologous) or different from (heterologous) those isolated from the infants' feces was highly inhibited by samples of infant serum collected both during the acute phase of the illness and upon discharge from the hospital. These data confirm the development of antibodies against EPEC adhesins and the cross-reaction between different EPEC serotypes. Cord serum inhibited the localized adherence of EPEC strains at different levels according to the serotype of the strain studied. These results suggest that the placental transfer of adhesin-related antibodies does not protect the newborn against EPEC infections, since half of our patients were less than 30 days old

Use of plasmid profiles to differentiate strains withi9n specific serotypes of classical enteropathogenic Escherichia coli

The usefulness of plasmid profile analysis to differentiate strains of enteropathogenic Escherichia coli (EPEC) was evaluated by studying 123 strains of the most prevalent serotypes causing infant diarrhea in the city of Säo Paulo, Brazil, i. e.,0111ab:H-, 0111ab:H2 and 0119:H6. No common profiles were found among strains of distinct serotypes. However, within each serotype, most of the strains were grouped within a few major profiles. More than 68% of the strainsof serotypes 0111ab:H- and 0111ab:H2 were included in 6 and 9 major profiles, respectively. In 0119:H6, abouth 48% of the strainswere included in 3 major profiles. This analysis suggewsts that only a few EPEC clones are causing infant diarrhea in Säo Paulo and revealed that the distribution of serotypes 0111 ab:H- and 0111ab:H2 during the one-year study was at least partly determined by small outbreaks of the most common profiles. We conclude that plasmid profile analysis is very useful to differentiate strasins within specific EPEC serotypes

Reuniao anatomo-clinica de Instituto da Crianca junho de 1981. / Anatomo-clinical meeting of the Instituto da Crianca-June 1981

Apresenta-se um caso de trombose de veia renal em recem-nascido possivelmente filho de mae diabetica internado com 6 dias de idade.O peso de nascimento foi 5.600g. O paciente foi admitido em pessimo estado geral, sendo submetido a exsanguineotransfusao. Quatro horas apos a internacao constatou-se convulsao generalizada controlada com diazepinico. Faleceu 16 horas apos a admissao, com sangramentos multiplos. A autopsia revelou trombose venosa renal bilateral recente, com enfartos hemorragicos, tromboembolias e hematomas, bem como broncopneumonia bilateral e impetigo.Sao discutidos os principais dados clinicos da trombose de veia renal em recem-nascidos

Salmonelose no periodo neonatal: analise de 55 casos. / Salmonellosis in the neonatal period: analysis of 55 cases

Os autores analisam a incidencia, a procedencia, o quadro clinico, os aspectos bacteriologicos, a terapeutica e as sequelas decorrentes da infeccao por Salmonella em 55 recem-nascidos. A contaminacao hospitalar e destacada assim como a importancia do aleitamento materno, o elevado custo operacional de suporte da doenca e a condicao de portador do microorganismo

Programa de tocoginecologia preventiva.Plano piloto de medicina integral. / Program of preventive obstetrics and ginecology. Pilot plan of integral medicine

E importante conscientizar os estudantes de medicina e os medicos de que e mais adequado atender o paciente fazendo o que e necessario de forma simples e eficiente, sem perda de qualidade, de que atender de modo sofisticado a demanda manifestada sem olhar diversos aspectos organicos do paciente como um todos

Insuficiencia renal aguda no recem-nascido: analise de 7 casos. / Acute kidney insufficiency in newborn infant: analysis of 7 cases

Os autores apresentam a analise clinica e laboratorial de 7 recem-nascidos com o diagnostico de insuficiencia renal aguda, estudados na Secao de Recem-Nascidos Externos do Instituto da Crianca do Hospital das Clinicas. Sao comentados as indicacoes, tecnica e resultados da dialise peritoneal. Em 5 casos, o disturbio funcional relacionou-se a desidratacao e/ou choque e/ou septicemia, e em 2 casos a processo organico

Drogas administradas a gestante e suas repercussoes hematologicas no recem-nascido. / Drug administration to pregnant women and its haematologic repercussions in the newborn infant

Os autores analisam as repercussoes, do ponto de vista hematologico para o recem-nascido, de drogas usadas pela gestante, destacando hemorragia, doenca hemolitica neonatal, plaquetopenia e metemoglobinemia. Comentam que o valor terapeutico das drogas deve ser avaliado com base nos possiveis efeitos adversos para o feto e para o recem-nato