RESUMO
The fertosphere, as the interfaces between fertilizer granular and soil particles, represents a key hotspot for nitrogen transformation processes, particularly for ammonia (NH3) and nitrous oxide (N2O) emissions. Understanding the heterogeneity of the fertosphere, especially when incorporating organic amendments like biochars, is crucial for predicting NH3 and N2O emissions after soil fertilization. In this study, we investigated the effects of three types of biochar (pristine, aged, and acid-washed biochar) on heterogeneity of fertosphere induced by localized urea application. pH-specific planar optodes were employed to visualize pH gradients in fertosphere hotspots with high spatial and temporal resolution. In addition, we conducted thorough measurements of the gradient distribution of electric conductivity (EC), mineral N, aqueous NH3 in soil and enzyme activities relevant to nitrification. Concurrently, NH3 and N2O emissions from the soil were continuously monitored at a high temporal resolution. Initially, urea-induced fertosphere exhibited significant NH3 emissions, primarily attributed to the pH elevation resulting from urea hydrolysis. However, after 6 days, NH3 emissions subsided, and there was a notable sharp increase in N2O emissions. Importantly, compared to urea application alone, the inclusion of pristine biochar led to a delay in soil pH decline with a 19% rise in NH3 emission. Aged biochar, characterized by a higher content of oxygen functional groups, demonstrated increased NH4+/NH3 adsorption capacity and enhanced ammonia monooxygenase (AMO) activity in soil, resulting in an 18% reduction in NH3 emission. While a slight decrease of 5% in NH3 cumulative emission was observed in the acid-washed biochar treatment. Notably, biochar could potentially promote nitrification-derived N2O emissions due to the accumulation of NH3 oxidation products (NH2OH). These findings could contribute to refining N transformation models for fertilized soils, and optimizing N fertilizer application strategies.
Assuntos
Carvão Vegetal , Fertilizantes , Fertilizantes/análise , Carvão Vegetal/química , Solo/química , Nitrogênio/química , Óxido Nitroso/análise , Ureia , Agricultura/métodosRESUMO
Polygonum cuspidatum (P. cuspidatum) is a traditional herbal medicine with a long history and proven efficacy in treating gout. However, due to the complexity of composition and extensive content distribution, the substance basis of its anti-gout effectiveness is still unclear. A strategy was proposed via integrating off-line two-dimensional liquid chromatography (2D-LC) and targeted rapid screening technology based on ultrafiltration-liquid chromatography-mass spectrometry (UF-LC/MS) and on-line high-performance liquid chromatography-2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (HPLC-ABTS) to accomplish high coverage and high throughput screening of anti-gout components from P. cuspidatum. As a result, twenty components were screened from P. cuspidatum extract with both xanthine oxidase (XOD) inhibitory activity and free radical scavenging activity, then were preliminarily identified by high-resolution electrospray ionization-quadrupole-time-of-flight mass spectrometer (ESI-Q-TOF/MS). The screened results were verified by the in vitro assays. Meanwhile, molecular docking further elucidated that the screened bioactive ingredients had favourable binding capabilities with XOD. The performance of this study can achieve high efficiency and high coverage screening of the anti-gout components from P. cuspidatum, which provides methodology and strategy support for the rapid screening of bioactive ingredients from complex medicinal plants.
Assuntos
Benzotiazóis , Fallopia japonica , Gota , Plantas Medicinais , Ácidos Sulfônicos , Cromatografia Líquida de Alta Pressão/métodos , Espectrometria de Massa com Cromatografia Líquida , Ultrafiltração/métodos , Simulação de Acoplamento MolecularRESUMO
Cemented gangue backfill pier-column (CGBP) which was made of coal gangue, fly ash, cement, and water is the supporting component of the goaf in partial backfill mining or constructional backfill mining for controlling the surface subsidence of coal mining. The width-height ratio and roof-floor strength directly affect the bearing capacity of CGBP under axial compression, which is essential for the design of CGBP. Herein, the effect of width-height ratio (1:3-1:1) on the mechanical characteristics of CGBP with different curing ages under uniaxial compression was system studied through experiment, and the damage process was analyzed by ultrasonic equipment and DIC. Based on the experimental results and discrete element theory, a three-phase numerical model for CGBP was established, which considered the real aggregate shape and distribution and the mechanical characteristics of each phase. Then, the effects of the end friction coefficient and the strength ratio of roof-CGBP-floor combination on the strength and failure characteristics of CGBP (large width-height ratio: 1:1-4:1) were investigated. The results show that CGBP shows the width-height ratio effect obviously and the strength and ductility increase with the increase of the width-height ratio, and the width-height ratio effect increases with the increase of curing age and strength ratio. The end friction constraint is the main reason for the width-height ratio effect, and the higher the friction coefficient is, the larger the width-height ratio effect shows, and the width-height ratio effect disappears without end friction constraint. The increase of the width-height ratio of CGBP and the strength ratio of the roof-CGBP-floor combination increases the strength of the combination. Whether the strength of the combination is greater than that of CGBP may have a roof-floor strength threshold or a strength ratio threshold, which are between 31.44-54.11 MPa and 3.75-6.44, respectively. When the strength of the roof and floor is different, the strength of the combination is mainly controlled by the weak carrier and increases with the increase of the strength of the weak carrier. The peak strain energy of CGBP and combination increases with the increase of end friction coefficient, width-height ratio, and strength of roof and floor. The experimental and simulation results can be used to guide the design of CGBP in constructional backfill mining or partial backfill mining.
Assuntos
Minas de Carvão , Minas de Carvão/métodos , Pressão , Água , Carvão Mineral/análise , Cinza de CarvãoRESUMO
The strip and column cemented gangue backfill bodies (CGBBs) are the main supporting components in the design of constructional backfill mining for coal mining, which determines the stability of goaf. Previous researches have mostly focused on the mechanical properties of column CGBB, but the mechanical properties of strip CGBB are still unclear. Herein, the uniaxial compression experiments for strip and column CGBBs were conducted to compare the failure properties. The acoustic emission (AE) and two types of resistivity monitoring were used to monitor the damage evolution. The effect of the length-height ratio on the mechanical characteristic of strip CGBB was analyzed by discrete element simulation. The results show that the strength and peak strain of strip CGBB under uniaxial compression is higher than those of column CGBB and the strip CGBB shows better ductility. The stress of column CGBB decreases significantly faster than that of strip CGBB at the post-peak stage. The strength and ductility of strip CGBB increase with the increase of length-height ratio. The strip CGBB is destroyed from both ends to the middle under uniaxial compression, and the core bearing area is reduced correspondingly. The AE signal evolution of CGBBs under uniaxial compression before the peak stress contains three stages, and the AE signals of strip CGBB at the peak stress will not rise sharply compared with column CGBB. The resistivity monitoring effect of the horizontally symmetrical conductive mesh is better than that of the axial. The horizontal resistivity increases gradually with the increase of stress under uniaxial compression, and increases sharply at the peak stress, and then drops after the peak stress. The damage constitutive models and the stability monitoring models of the CGBBs are established based on the experimental results. This work would be instructive for the design and stability monitoring of CGBB.
Assuntos
Minas de Carvão , Acústica , Minas de Carvão/métodos , PressãoRESUMO
Constructional backfill mining with cemented gangue backfill column can solve the environmental issues caused by mining activities and the accumulation of waste gangue at a low cost. To study the deformation and instability properties of cemented gangue backfill columns during the advancement of coal mining face, five step-by-step loading paths were adapted to mimic the different loading processes of the roof. The lateral deformation at different heights and axial deformation of the sample were monitored. The results show that the deformation and instability of the backfill column have the properties of loading paths and are affected by the step-by-step loading path. When stress-strength ratio (SSR) is less than 0.6, the lateral of backfill column shrinks during the creeping process. In high-stress levels, lateral creep strain develops faster than axial creep strain. The backfill column has characteristics of axial creep hardening and lateral creep softening during the step-by-step loading process. The instantaneous deformation modulus and instantaneous Poisson's ratio show an upward trend. The strength of backfill column under the step-by-step load is related to loading paths and is no less than uniaxial compressive strength. The non-uniformity of the lateral deformation of backfill column leads to excessive localized deformation that mainly occurs in the middle, causing the overall instability. The development of cracks of backfill column under step-by-step load could be divided into 4 stages according to SSR. Under different step-by-step loading paths, the axial creep strain rate is nearly a constant before entering the accelerated creep stage. A nonlinear creep constitutive model with a creep strain rate trigger was proposed to depict the development of axial strain under step-by-step load. This research could provide a scientific reference for the design of the advancing distance and cycle for the hydraulic support, and reinforcement of the backfill column.
Assuntos
Minas de Carvão , Força CompressivaRESUMO
The relationships between metal uptake and antioxidant enzyme activities or a response to membrane lipid peroxidation (i.e., malondialdehyde production) in Chlorella vulgaris exposed to Cu and Cd compounds singly and in combination were investigated. The results showed that bioaccumulation of a single metal was influenced by the presence of the other metal. The activities of superoxide dismutase and peroxidase increased to more than fivefold of the control after exposure to Cu(1.5 µM) alone or to Cu(1.5 µM) with Cd mixtures. Malondialdehyde levels in C. vulgaris also increased to approximately twofold of the control after exposure to high concentration of Cu(1.5 µM) alone or to Cu and Cd mixtures. However, Cd alone did not significantly increase the levels of antioxidant enzymes or malondialdehyde.