In vivo lymph node mapping by Cadmium Tellurium quantum dots in rats.

BACKGROUND: Intraoperative lymph node mapping (LNM) is highly significant for many surgeries in patients with cancer. Many types of tracers are currently used, but the ideal method has not yet been identified. We aimed to identify a stable lymphatic drainage pathway in an animal model and compared the effects of quantum dots (QD), a new fluorescent tracer, with those of methylene blue in intraoperative LNM. MATERIALS AND METHODS: Indian ink (0.2 mL) was subcutaneously injected into the plantar metatarsal regions of six Sprague-Dawley rats. After 2 wk of incubation and subsequent dissection, the potentially stained LNs were examined pathologically to identify the lymphatic drainage pathway. After applying anesthesia, 0.1 mL methylene blue (2%) and QD (1 mg/mL) were injected into the plantar metatarsal regions of six rats for intraoperative LNM. The QD group was observed with a near-infrared imaging system, and the methylene blue group was directly observed. Drainages were recorded at 5, 10, 30, 60, and 120 min and at 1 d. RESULTS: Two three-level drainage pathways, that is, a peripheral drainage (popliteal LNs, inguinal LNs, and axillary LNs) and a central drainage (popliteal lymph node [LN], iliac LN, and renal LN) pathways were identified. Both methylene blue and QD stained the sentinel lymph node (SLNs) quickly, but methylene blue was difficult to identify in the deep tissues and the LNs beyond the SLN. Furthermore, the blue-stained LNs remain dyed for only 2 h. In contrast, the QDs exhibited high target-to-background ratios in both the SLNs and the following LNs. Additionally, the fluorescence lasted from 5 min-1 d after injection. CONCLUSIONS: An ideal lymphatic drainage model was found. QDs are excellent tracers for intraoperative LNM compared with methylene blue. Near infrared fluorescent imaging is a promising LNM method for clinical practice.

Silica composite nanoparticles containing fluorescent solid core and mesoporous shell with different thickness as drug carrier.

Nonporous silica transitional approach was employed to create core-shell architectural nanocomposites, which performed particularly well in morphology and controllable synthesis. The silica nanocomposites containing fluorescent solid SiO2 core and mesoporous silica shell (F-nSiO2/mSiO2) presented distinct structures of narrow size distribution, stable and shell thickness independent fluorescence, and high specific surface area. Furthermore, the thickness of mesoporous shell could be precisely tailored by the amount of TEOS and solid SiO2 seeds. Drug delivery study of F-nSiO2/mSiO2 with different mesoporous thicknesses were carried out, and Peppas equation was adopted to demonstrate the controlled releasing mechanism of doxorubicin (DOX). The diffusion rate of DOX from F-nSiO2/mSiO2 nanocomposites depended on the thickness of mesoporous shell and electrostatic interaction between drug and silanol group, which facilitated an enhanced drug releasing activity at pH 5.5 than 7.4. What's more, particles loaded DOX showed similar cytotoxicity compared with pure DOX, while no obvious cytotoxicity of carrier was observed in MTT tests for blank particles. These characteristics mentioned above implied that core/shell structured F-nSiO2/mSiO2 had a great potential for controlled drug delivery system.