RESUMO
Five simple and rapid methods for evaluation of sorghum and pearl millet transgenics resistant to herbicide phosphinothricin (used as selectable marker) were studied. For rapid in vitro selection, three assays (establishment of sensitivity curves for embryogenic calli, determination of lethal doses for seed germination, and a rapid screening of cut young leaves based on the colour change of the medium) were established. For rapid screening of transgenic progeny, effects of in vivo Basta leaf spray and dip tests were studied at three different morphological stages. For all the above assays, LD50, and LD100 values were higher for pearl millet than sorghum. However, in both the crops, genotype effect was not significant. The assays standardized in the study were found to be effective for rapid, economical and mass-scale identification and characterization of transgenic plants of sorghum and pearl millet.
Assuntos
Aminobutiratos/farmacologia , Resistência a Medicamentos , Herbicidas/farmacologia , Pennisetum/genética , Sorghum/genética , Transgenes/efeitos dos fármacos , Bioensaio/economia , Germinação , Glutamato-Amônia Ligase/antagonistas & inibidores , Pennisetum/efeitos dos fármacos , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas , Sorghum/efeitos dos fármacosRESUMO
Infection with the Human Immunodeficiency Virus (HIV) has an increasing, direct and significant impact on the hospital, especially the emergency services. Out of a total of 39,876 patients screened, 1061 patients were reactive for anti-HIV antibodies. The incidence of the infection showed a remarkable progression from 0.89% in 1992-1993 to 5.6% in 1997, among the seropositive patients. The number of patients with signs and symptoms related to HIV infection has also shown a 2 fold rise (from 42% in 1992-1993 to 87% in 1997). The prevalence of HIV 1 in the seropositive patients is 93%, HIV 2 alone is 2.3% and 3.1% had a mixed infection with both HIV 1 and 2. Secondary infection with Mycobacteria ranked high (25%) among the symptomatic patients, with pulmonary and the disseminated varieties being more common.
Assuntos
Infecção Hospitalar/diagnóstico , Infecção Hospitalar/epidemiologia , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , HIV-1/isolamento & purificação , HIV-2/isolamento & purificação , Adolescente , Adulto , Distribuição por Idade , Idoso , Criança , Pré-Escolar , Feminino , Hospitais Gerais , Humanos , Incidência , Índia/epidemiologia , Lactente , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Índice de Gravidade de Doença , Distribuição por Sexo , Análise de SobrevidaRESUMO
A case of necrotizing fasciitis of the anterior abdominal wall caused by the zygomycete Apophysomyces elegans in a healthy male following inguinal herniorrhaphy is reported. The portal of entry of the fungus into the incised skin and subcutaneous tissues was probably through either contaminated surgical sutures or postoperative surgical dressings. Broad, aseptate fungal hyphae were seen in the necrosed tissues with an associated necrotizing vasculitis. Extensive tissue debridements and a low dose of amphotericin B were not successful in controlling the rapid invasion of the tissues by the fungus.
