RESUMO
OBJECTIVE: To investigate the fitness of serum apolipoprotein M (apoM) concentration as a marker for maturity-onset diabetes of the young 3 (MODY3). STUDY DESIGN AND SUBJECTS: This study consisted of two parts. A family study included 71 carriers of the P291fsinsC mutation in hepatocyte nuclear factor-1alpha (HNF-1alpha) from the Finnish Botnia study, 53 of whom were diabetic, and 75 matched family controls. A second, case-control study included 24 MODY3 patients, 17 healthy MODY3 mutation carriers, 11 MODY1 patients, 18 type 2 diabetes patients and 19 healthy control individuals. Subjects in the case-control study were recruited from the Botnia study or the Clinic of Endocrinology, Malmö University Hospital. Serum apoM levels were measured using a novel ELISA based on two monoclonal apoM antibodies. RESULTS: In the family study, mean serum apoM was 10% lower in female carriers of the P291fsinsC mutation compared to the family controls (P = 0.0058), a difference which remained significant after adjustment for diabetes status. There was no observed difference between groups for men. In the case-control study, no significant difference in apoM concentration was observed between MODY3 and type 2 diabetes patients, neither before nor after adjustment for total cholesterol. CONCLUSIONS: Female carriers of the P291fsinsC mutation in HNF-1alpha displayed slightly lower apoM serum levels. This difference is too small for apoM to be reliably employed as a biomarker for HNF-1alpha mutation status.
Assuntos
Apolipoproteínas/sangue , Diabetes Mellitus Tipo 2/sangue , Adulto , Apolipoproteínas M , Biomarcadores/sangue , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/genética , Ensaio de Imunoadsorção Enzimática , Feminino , Predisposição Genética para Doença , Fator 1-alfa Nuclear de Hepatócito/genética , Heterozigoto , Humanos , Lipocalinas , Masculino , Pessoa de Meia-Idade , Mutação , Valor Preditivo dos Testes , Fatores SexuaisRESUMO
A total of 1345 elementary schoolchildren from grades 1-3 (aged 7-9 years) were examined and treated by community health center dentists during 1975-1977. The combined effect of fissure sealing and topical fluoride therapy with 2% NaF gel resulted over the 2 years in an increase in the proportion of caries-free permanent first molars from 50% to 81% in the 1st grade. The mean DMFS scored from the same teeth decreased from 2.8 to 0.8. In the age group born in 1968 the increment of new carious surfaces was 0.28 DFS/year with sealing and 1.13 DFS/year without.
