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1.
Ultrasound Obstet Gynecol ; 43(4): 444-51, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24105723

RESUMO

OBJECTIVE: To explore the association between clinical training characteristics and trainees' level of confidence in performing ultrasound scans independently. METHODS: A cross-sectional e-survey was distributed to members of the national societies of junior obstetricians/gynecologists in Denmark, Sweden and Norway (n = 973). Multiple linear regression models were used to explore the effect that amount of time spent in specialized ultrasound units and clinical experience had on trainees' confidence in performing ultrasonography independently. Exploratory factor analysis was used to identify factors that contributed to trainees' confidence in performing ultrasonography. Trainees' ultrasound confidence was finally compared with their expected levels of performance. RESULTS: Of the 682 respondents (response rate 70.1%), 621 met the inclusion criteria. Clinical experience and time spent in specialized ultrasound units were predictors of trainees' confidence in performing ultrasonography independently (P < 0.001). Trainees required more than 24 months of clinical experience and 12-24 days of training in specialized ultrasound units in order to feel confident about performing transvaginal and transabdominal ultrasound scans independently. Three factors were related to ultrasound confidence: technical aspects, image perception and integration of scan into patient care. There were significant differences between trainees' level of confidence and their expected levels of performance (P < 0.001). CONCLUSIONS: Clinical experience and time spent in specialized ultrasound units were predictors of trainees' confidence in performing ultrasonography independently. Discrepancies between trainees' confidence and their expected levels of performance raised concerns about the adequacy of current ultrasound training programs.


Assuntos
Competência Clínica , Ginecologia , Obstetrícia , Ultrassom , Adulto , Atitude do Pessoal de Saúde , Competência Clínica/normas , Estudos Transversais , Dinamarca , Educação Médica Continuada , Educação de Pós-Graduação em Medicina , Feminino , Ginecologia/educação , Ginecologia/normas , Humanos , Masculino , Pessoa de Meia-Idade , Noruega , Obstetrícia/educação , Obstetrícia/normas , Gravidez , Inquéritos e Questionários , Suécia , Ultrassom/educação , Ultrassom/normas
2.
Appl Environ Microbiol ; 74(8): 2441-6, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18310432

RESUMO

We investigated the degree of physiological damage to bacterial cells caused by optical trapping using a 1,064-nm laser. The physiological condition of the cells was determined by their ability to maintain a pH gradient across the cell wall; healthy cells are able to maintain a pH gradient over the cell wall, whereas compromised cells are less efficient, thus giving rise to a diminished pH gradient. The pH gradient was measured by fluorescence ratio imaging microscopy by incorporating a pH-sensitive fluorescent probe, green fluorescent protein or 5(6)-carboxyfluorescein diacetate succinimidyl ester, inside the bacterial cells. We used the gram-negative species Escherichia coli and three gram-positive species, Listeria monocytogenes, Listeria innocua, and Bacillus subtilis. All cells exhibited some degree of physiological damage, but optically trapped E. coli and L. innocua cells and a subpopulation of L. monocytogenes cells, all grown with shaking, showed only a small decrease in pH gradient across the cell wall when trapped by 6 mW of laser power for 60 min. However, another subpopulation of Listeria monocytogenes cells exhibited signs of physiological damage even while trapped at 6 mW, as did B. subtilis cells. Increasing the laser power to 18 mW caused the pH gradient of both Listeria and E. coli cells to decrease within minutes. Moreover, both species of Listeria exhibited more-pronounced physiological damage when grown without shaking than was seen in cells grown with shaking, and the degree of damage is therefore also dependent on the growth conditions.


Assuntos
Bacillus subtilis/efeitos da radiação , Escherichia coli/efeitos da radiação , Listeria/efeitos da radiação , Pinças Ópticas , Membrana Celular/metabolismo , Lasers , Microscopia de Fluorescência/métodos , Força Próton-Motriz
3.
Resuscitation ; 75(1): 153-60, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17467869

RESUMO

UNLABELLED: Robust assessment of Advanced Life Support (ALS) competence is paramount to the credibility of ALS-provider certification and for estimating the learning outcome and retention of ALS competence following the courses. The European Resuscitation Council (ERC) provides two sets of MCQs and four Cardiac Arrest Simulation Test (CASTest) scenarios for the assessments according to guidelines 2005. AIMS: To analyse the reliability and validity of the individual sub-tests provided by ERC and to find a combination of MCQ and CASTest that provides a reliable and valid single effect measure of ALS competence. METHODS: Two groups of participants were included in this randomised, controlled experimental study: a group of newly graduated doctors, who had not taken the ALS course (N=17) and a group of students, who had passed the ALS course 9 months before the study (N=16). Reliability in terms of inter-rater agreement and generalisability across skills scenarios were estimated. Validity was studied in terms of equality of test difficulty and ability to discriminate performance between the groups. RESULTS: Inter-rater agreement on checklist scores were generally high, Intraclass Correlation Coefficients between 0.766 and 0.977. Inter-rater agreements on pass/fail decisions were not perfect. The one MCQ test was significantly more difficult than the other. There were no significant differences between CASTests. Generalisability theory was use to identify a composite of MCQ and CASTest scenarios that possessed high reliability, equality of test sets, and ability to discriminate between the two groups of supposedly different ALS competence. CONCLUSIONS: ERC sub-tests of ALS competence possess sufficient reliability and validity. A combined ALS score with equal weighting of one MCQ and one CASTest can be used as a single measurement of ALS competence.


Assuntos
Suporte Vital Cardíaco Avançado/educação , Competência Clínica , Avaliação Educacional/métodos , Certificação , Europa (Continente) , Humanos , Variações Dependentes do Observador , Reprodutibilidade dos Testes
4.
Ugeskr Laeger ; 161(9): 1273-4, 1999 Mar 01.
Artigo em Dinamarquês | MEDLINE | ID: mdl-10083825

RESUMO

A case story of dopa-responsive childhood dystonia is presented. The symptoms, diagnostics, pitfalls and treatment are discussed.


Assuntos
Dopaminérgicos/uso terapêutico , Distonia/tratamento farmacológico , Levodopa/uso terapêutico , Criança , Diagnóstico Diferencial , Distonia/diagnóstico , Feminino , Humanos
5.
Ugeskr Laeger ; 154(17): 1180-4, 1992 Apr 20.
Artigo em Dinamarquês | MEDLINE | ID: mdl-1604746

RESUMO

Since August 1988, in Odense Hospital, electric spinal cord stimulation (SCS) has been employed for the treatment of pain in patients with confirmed ischaemic heart disease who suffer from incapacitating angina pectoris despite maximal medical/surgical treatment. The object of the present investigation was to assess not only the social economic consequences of SCS treatment (cost-utility analysis) but also altered quality of life in SCS patients (perception of pain, mobility, function in daily life and physical activity). Sixteen consecutive SCS patients all of whom were resident in the County of Funen and who were submitted to implantation of an SCS system during the period August 1988 to December 1989, participated in this investigation. The results are based on data from the year prior to SCS implantation compared with the subsequent time with SCS treatment. Saving was found at hospital level (reduction in number of admissions) og 40,200 Danish crowns/annum/patient (approximately IJ 3,000) (1989 prices), and for non-hospital related expenses a corresponding saving of 16,289 Danish crowns/annum/patient (approximately IJ 1,600) was found mainly on account of reduction in the amount of home nursing required. The total saving was found to constitute 56,489 Danish crowns/annum/patient (approximately IJ 5,600). In addition, improvements were registered in all respects which constituted assessment of the quality of life of the patients.


Assuntos
Angina Pectoris/terapia , Terapia por Estimulação Elétrica/economia , Medula Espinal/fisiologia , Idoso , Angina Pectoris/economia , Angina Pectoris/psicologia , Redução de Custos , Análise Custo-Benefício , Dinamarca , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Qualidade de Vida
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