Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
Clin Vaccine Immunol ; 19(7): 1027-37, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22623650

RESUMO

Chronic Hepatitis C virus (HCV) infection has been linked with B cell lymphoproliferative disorders and several autoimmune-related diseases. The mechanisms of how chronic viral infection affects B cell development and predisposes the patients to autoimmune manifestations are poorly understood. In this study, we established an experimental system to probe the B cell responses and characterize the antibodies from chronic-HCV-infected individuals. We identified an unusual polyclonal expansion of the IgM memory B cell subset in some patients. This B cell subset is known to be tightly regulated, and autoreactive cells are eliminated by tolerance mechanisms. Genetic analysis of the immunoglobulin (Ig) heavy chain variable gene (V(H)) sequences of the expanded cell population showed that the levels of somatic hypermutation (SHM) correlate with the extent of cell expansion in the patients and that the V(H) genes exhibit signs of antigen-mediated selection. Functional analysis of the cloned B cell receptors demonstrated autoreactivity in some of the expanded IgM memory B cells in the patients which is not found in healthy donors. In summary, this study demonstrated that, in some patients, chronic HCV infection disrupts the tolerance mechanism that normally deletes autoreactive B cells, therefore increasing the risk of developing autoimmune antibodies. Long-term follow-up of this expanded B cell subset within the infected individuals will help determine whether these cells are predictors of more-serious clinical manifestations.


Assuntos
Doenças Autoimunes/imunologia , Linfócitos B/imunologia , Hepatite C Crônica/imunologia , Tolerância Imunológica , Adulto , Idoso , Autoanticorpos/sangue , Doenças Autoimunes/patologia , Subpopulações de Linfócitos B/imunologia , Feminino , Humanos , Imunoglobulina G/sangue , Cadeias Pesadas de Imunoglobulinas/genética , Imunoglobulina M/sangue , Região Variável de Imunoglobulina/genética , Memória Imunológica , Masculino , Pessoa de Meia-Idade
2.
Cell Metab ; 12(5): 533-44, 2010 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-21035763

RESUMO

High-fat-diet (HFD)-induced obesity is a major contributor to diabetes and cardiovascular disease, but the underlying genetic mechanisms are poorly understood. Here, we use Drosophila to test the hypothesis that HFD-induced obesity and associated cardiac complications have early evolutionary origins involving nutrient-sensing signal transduction pathways. We find that HFD-fed flies exhibit increased triglyceride (TG) fat and alterations in insulin/glucose homeostasis, similar to mammalian responses. A HFD also causes cardiac lipid accumulation, reduced cardiac contractility, conduction blocks, and severe structural pathologies, reminiscent of diabetic cardiomyopathies. Remarkably, these metabolic and cardiotoxic phenotypes elicited by HFD are blocked by inhibiting insulin-TOR signaling. Moreover, reducing insulin-TOR activity (by expressing TSC1-2, 4EBP or FOXO), or increasing lipase expression-only within the myocardium-suffices to efficiently alleviate cardiac fat accumulation and dysfunction induced by HFD. We conclude that deregulation of insulin-TOR signaling due to a HFD is responsible for mediating the detrimental effects on metabolism and heart function.


Assuntos
Gorduras na Dieta/efeitos adversos , Proteínas de Drosophila/metabolismo , Drosophila/metabolismo , Cardiopatias/etiologia , Obesidade/etiologia , Serina-Treonina Quinases TOR/metabolismo , Animais , Drosophila/genética , Proteínas de Drosophila/genética , Coração/fisiopatologia , Cardiopatias/complicações , Cardiopatias/metabolismo , Humanos , Insulina/metabolismo , Doenças Metabólicas/genética , Mutação , Obesidade/complicações , Obesidade/metabolismo , Fenótipo , Transdução de Sinais , Serina-Treonina Quinases TOR/genética , Triglicerídeos/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA