Clinical Relevance of Gut Microbiota Alterations under the Influence of Selected Drugs-Updated Review.

As pharmacology and science progress, we discover new generations of medicines. This relationship is a response to the increasing demand for medicaments and is powered by progress in medicine and research about the respective entities. However, we have questions about the efficiency of pharmacotherapy in individual groups of patients. The effectiveness of therapy is controlled by many variables, such as genetic predisposition, age, sex and diet. Therefore, we must also pay attention to the microbiota, which fulfill a lot of functions in the human body. Drugs used in psychiatry, gastroenterology, diabetology and other fields of medicine have been demonstrated to possess much potential to change the composition and probably the function of the intestinal microbiota, which consequently creates long-term risks of developing chronic diseases. The article describes the amazing interactions between gut microbes and drugs currently used in healthcare.

Synthesis and Significance of Arachidonic Acid, a Substrate for Cyclooxygenases, Lipoxygenases, and Cytochrome P450 Pathways in the Tumorigenesis of Glioblastoma Multiforme, Including a Pan-Cancer Comparative Analysis.

Glioblastoma multiforme (GBM) is one of the most aggressive gliomas. New and more effective therapeutic approaches are being sought based on studies of the various mechanisms of GBM tumorigenesis, including the synthesis and metabolism of arachidonic acid (ARA), an omega-6 polyunsaturated fatty acid (PUFA). PubMed, GEPIA, and the transcriptomics analysis carried out by Seifert et al. were used in writing this paper. In this paper, we discuss in detail the biosynthesis of this acid in GBM tumors, with a special focus on certain enzymes: fatty acid desaturase (FADS)1, FADS2, and elongation of long-chain fatty acids family member 5 (ELOVL5). We also discuss ARA metabolism, particularly its release from cell membrane phospholipids by phospholipase A2 (cPLA2, iPLA2, and sPLA2) and its processing by cyclooxygenases (COX-1 and COX-2), lipoxygenases (5-LOX, 12-LOX, 15-LOX-1, and 15-LOX-2), and cytochrome P450. Next, we discuss the significance of lipid mediators synthesized from ARA in GBM cancer processes, including prostaglandins (PGE2, PGD2, and 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2)), thromboxane A2 (TxA2), oxo-eicosatetraenoic acids, leukotrienes (LTB4, LTC4, LTD4, and LTE4), lipoxins, and many others. These lipid mediators can increase the proliferation of GBM cancer cells, cause angiogenesis, inhibit the anti-tumor response of the immune system, and be responsible for resistance to treatment.

Concentrations of Mg, Ca, Fe, Cu, Zn, P and anthropometric and biochemical parameters in adults with chronic heart failure.

BACKGROUND: The study investigated the relationship between the concentrations of Mg, Ca, Fe, Cu, Zn, P and anthropometric and biochemical parameters in the blood serum of patients with heart failure (HF) and the potential influence on the development and progression of HF. MATERIAL & METHODS: The study included 214 patients (155 men and 59 women), aged 40-87 years, presenting symptoms or signs typical of HF (according to the NYHA functional classification). Serum concentrations were determined for Mg, Ca, Fe, Cu, Zn, P, C-reactive protein (CRP), creatinine, urea, triglyceride levels (TG), total cholesterol (CH), high density protein (HDL), low density protein (LDL). The levels of macro-and microminerals were analysed using inductively coupled serum optical emission spectrometry (ICP-OES). RESULTS: Our study confirmed the role of known risk factors in the development of heart failure, including: overweight, diabetes, hypertension, high triglycerides (TG), high total cholesterol (CH), high levels of low density protein (LDL) and reduced levels of high density protein (HDL), high CRP, high creatinine. Moreover, deficient serum concentrations of Mg (47% of the studied men and 54% of the women) and Cu (in 44% of men and more than 30% of women) were observed, as well as subnormal serum Fe (2% of women) and Zn (1% of men). Elevated serum Ca was found in 50% of men and 49% of women. In 44% of the studied men and 52% of the studied women, P levels in serum were also above-average. The study revealed a significant positive correlation between serum levels of Ca and Mg, and also Ca and Cu in women. In men, serum Cu was positively correlated with Mg and Ca concentrations. In patients from group 1 (NYHA I-II), Mg content was positively correlated with Ca and Cu. In this patient group, Ca was also positively associated with Cu content in serum. In group 2 (NYHA III-IV), serum Mg concentration was significantly positively correlated with that of Cu and Ca. CONCLUSIONS: Changes in the serum concentrations of macro-and microminerals may significantly affect the severity of HF in Polish patients.

Analysis of Antioxidant Capacity and Antimicrobial Properties of Selected Polish Grape Vinegars Obtained by Spontaneous Fermentation.

Nowadays, products of natural origin with health-promoting properties are increasingly more common. Research shows that fruit vinegars can be a source of compounds with antioxidant activity. Research on the total antioxidant capacity, total phenolic content, and antimicrobial properties against Staphylococcus aureus, Escherichia coli, and Candida albicans of grape vinegars were conducted. Moreover, gas chromatography was used to measure acetic acid content in the vinegars. The research material consisted of vinegars produced from five different grape varieties. For each variety, two variants were prepared: with and without the addition of sugar in the fermentation process. The highest antimicrobial activity against all micro-organisms was observed in vinegar produced from Solaris grapes with added sugar. The highest polyphenol content was observed in vinegar produced from the Prior grape variety with added sugar and the highest total antioxidant capacity is the Johanniter grape variety with added sugar. The vinegars examined in this study differed, depending on grape variety, in terms of antimicrobial properties, antioxidant capacity, total phenolic content, as well as acetic acid content. Sugar addition caused significant differences in the antioxidant capacity of vinegar samples.

Morphological variability and genetic diversity in Carex buxbaumii and Carex hartmaniorum (Cyperaceae) populations.

BACKGROUND: Carex buxbaumii and C. hartmaniorum are sister species of the clade Papilliferae within the monophyletic section Racemosae. An unambiguous identification of these species is relatively difficult due to the interspecific continuum of some morphological characters as well as the intraspecific variability. The study was aimed at determining the range of variability, both morphological and genetic, within and between these two closely related and similar species. METHODS: The sedges were collected during botanical expeditions to Armenia, Estonia, the Netherlands, and Poland. The morphological separation of the two species and their populations was tested using the Discriminant Function Analysis (DFA). The genetic variability of the 19 Carex populations was assessed in the presence of eight Inter Simple Sequence Repeat (ISSR) primers. RESULTS: Results of the study indicate a considerable genetic affinity between the two sedge species (mean Si = 0.619). However, the populations of C. hartmaniorum are, morphologically and genetically, more homogenous than the populations of C. buxbaumii. Compared to C. hartmaniorum, C. buxbaumii usually has wider leaf blades, a shorter inflorescence, a lower number of spikes which are shorter, but wider, and longer bracts and utricles. The AMOVA showed a larger variation between the populations of C. buxbaumii, representing 25.65% of the total variation in the taxon. Two populations of C. buxbaumii (from Poland and Estonia) are separated from the remaining populations, both genetically and morphologically; their individuals show shorter utricles and glumes, compared to the typical specimens of C. buxbaumii, and correspond with the morphology of putative infraspecific taxa described by Cajander (var. brevisquamosa and var. confusa). CONCLUSIONS: The taxonomic status of the putative infraspecific taxa within C. buxbaumii requires further studies throughout the distribution range of C. buxbaumii, addressing habitats, morphology and genetics (including a chromosome count or a combination of different genetic methods), particularly as the variability in C. buxbaumii may be associated with the species' polyploid origin.

Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method.

Coffee, being one of the world's most popular beverages, is a rich source of dietary antioxidants. The aim of this study was to determine the mineral content and antioxidant activity as well as acidity of coffee beverages depending on the brewing technique. We tested coffee brews made and served at a popular urban coffee shop (Szczecin, Poland). Five coffee brewing techniques were used: Aeropress, drip, espresso machine, French press, and simple infusion. Our findings showed that the brewing method had a significant effect on all parameters tested in the study. The antioxidant activity of the beverages was high (31%-42% inhibition of DPPH (2,2-diphenyl-1-picrylhydrazyl); reduction potential from 3435.06 mol Fe3+/mL to 4298.19 mol Fe3+/mL). Polyphenolic content ranged from 133.90 g (French press) to 191.29 g of gallic acid/L (Aeropress brew), depending on the coffee extraction method. Mineral content was also found to differ between brewing methods. Coffees prepared by simple infusion and Aeropress provided a valuable source of magnesium, manganese, chromium, cobalt, and potassium, whereas the drip brew was found to be a good source of silicon.

Fatty acid levels alterations in THP-1 macrophages cultured with lead (Pb).

OBJECTIVE: As cardiovascular events are one of the main causes of death in developed countries, each factor potentially increasing the risk of cardiovascular disease deserves special attention. One such factor is the potentially atherogenic effect of lead (Pb) on lipid metabolism, and is significant in view of the still considerable Pb environmental pollution and the non-degradability of Pb compounds. METHODS: Analysis of saturated fatty acids (SFA) (caprylic acid (C8:0), decanoic acid (C10:0), lauric acid (C12:0), tridecanoic acid (C13:0), myristic acid (C14:0), pentadecanoic acid (C15:0), palmitic acid (C16:0), heptadecanoic acid (C17:0), stearic acid (C18:0), and behenic acid (C22:0)), monounsaturated fatty acid (MUFA) (palmitoleic acid (C16:1), oleic acid (18:1w9), trans-vaccenic acid (C18:1 trans11)), and polyunsaturated fatty acid (PUFA) (linoleic acid (C18:2n6), gamma-linolenic acid (C18:3n6), arachidonic acid (C20:4n6)), was conducted by gas chromatography. Analysis of stearoyl-CoA desaturase (SCD), fatty acid desaturase 1 (FADS1) and fatty acid desaturase 2 (FADS2) expression was performed using qRT-PCR. Oxidative stress intensity (malondialdehyde - MDA concentration) was measured using spectrophotometric method. Intracellular generation of reactive oxygen species (ROS) in macrophages was visualized by fluorescence microscopy and quantitatively measured by plate reader. RESULTS: Pb caused quantitative alterations in FAs profile in macrophages; the effect was Pb-concentration dependent and selective (i.e. concerned only selected FAs). In general, the effect of Pb was biphasic, with Pb levels of 1.25 µg/dL and 2.5 µg/dL being stimulatory, and 10 µg/dL being inhibitory on concentrations of selected FAs. The most potent Pb concentration, resulting in increase in levels of 9 FAs, was 2.5 µg/dL, the Pb-level corresponding to the mean blood Pb concentrations of people living in urban areas not contaminated by Pb. Pb was found to exert similar, biphasic effect on the expression of FADS1. However, Pb decreased, in a concentration-dependent manner, the expression of SCD and FADS2. Pb significantly increased MDA and ROS concentration in macrophages. CONCLUSION: Environmental Pb exposure might be a risk factor resulting in alterations in FAs levels, oxidative stress and increased MDA concentration in macrophages, which might lead to the formation of foam cells and to inflammatory reactions.

The influence of the place of residence, smoking and alcohol consumption on bone mineral content in the facial skeleton.

BACKGROUND: Environmental factors exert their influence on the living organism throughout ontogeny. More and more often, researchers find correlations between specific environmental factors and the so-called diseases of affluence. Deficits and excess of essential elements also leave their mark on the skeleton. AIM: To investigate the influence of alcohol consumption, tobacco smoking and place of residence, according to sex and calendar age, on the concentrations of micro-, macro- and toxic elements in human facial bones. MATERIAL & METHODS: Patients undergoing surgical treatment were examined for the mineral content in the collected bone material. The bone contents of the following elements were determined: Ca, K, Mg, Na, P, Fe, Zn, Mo, Ba, Mn, Li, Be, Co, B, Sr, Cr, Pb, Cd, Ni, and Al, depending on the type of facial bone, sex, calendar age, alcohol consumption, tobacco smoking and place of residence. RESULTS: Sex and alcohol consumption showed the highest degree of correlation with the content of the minerals included in the study. Alcohol drinking was found to exert the strongest influence on women's bodies, the highest number of statistically significant correlations was demonstrated between the content of minerals in the examined bones and alcohol drinking in women. Other factors included in the analysis had a different impact on men and women, the concentrations of elements included in the study differed depending on age, tobacco smoking and place of residence. CONCLUSIONS: The observed differences in the element mineral composition of the human facial skeleton may be explained by developmental specifics and functional adaptation. However, general biological characteristics (sex, age), environmental factors (place of residence), as well as smoking and alcohol use may exert significant influence on the concentrations of micro-, macro- and toxic elements in particular regions of the human skeleton. The impact of environmental factors is a very complex phenomenon, which may be stronger or more subtle, leaving its mark on the bone structure. The environmental factors included in the analysis had a different influence on men than women.

Influence of Socio-Economic Status on Lung Function in Male Adolescents in Tanzania.

A lower socio-economic status (SES) associates with increased morbidity and mortality. The goal of this study was to define the influence of SES on lung function in indigenous male adolescents (aged 12.8-24.5 years) in Tanzania. The subjects were subdivided into two age groups: <17.5 (n = 189) and ≥ 17.5 years of age (n = 101). They underwent anthropometric and spirometry evaluation. The SES was assessed on the basis of economic family resources and the subjects' nutritional status, and was stratified into three categories: SES-1 (low), SES-2 (average), and SES-3 (high). Relative contributions of body mass index, chest expansion/mobility, waist-to-chest ratio, and SES to spirometry variables were statistically determined. We found significant differences in forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) in the older, but not younger age group, by the SES category. The adolescents aged ≥17.5 in high-SES-3 category had FEV1 and FVC greater by 29.3% and 42.5%, respectively, than those in low-SES-1 category in the same age group. There also were differences between the two age groups. The mean FEV1 and FVC in adolescents of high-SES-3 category, aged ≥17.5, were higher by 65.3% and 82.2%, respectively, than those in the corresponding SES-3 in adolescents aged <17.5. In younger adolescents, lung function variables associated mostly with body build parameters, whereas in older adolescents with SES. The study revealed a significant dependence of lung function, and thus plausibly a greater resistance to diseases, on the socio-economic status. We conclude that the socio-economic status be taken into consideration in the assessment of lung function in epidemiological and public health programs, particularly in the context of the developing countries, to obtain the most reliable results.

The multi-ethnic global lung initiative 2012 and Third National Health and Nutrition Examination Survey reference values do not reflect spirometric measurements in Black boys and men from Tanzania.

The interpretation of spirometric results of Black Africans according to reference standards based on data from outside their native environment may lead to the wrong conclusions. This article aims to characterize the ventilatory capacity of boys and men from Tanzania according to forced expiratory volume in one second (FEV1 ), forced vital capacity (FVC), peak expiratory flow (PEF) and FEV1 /FVC based on the collected anthropological material and to compare them to NHANES III, Third National Health and Nutrition Examination Survey (NHANES III) African American predicted values and GLI2012 equations. The analysis included spirometric measurements of n = 295 participants from Tanzania. Pearson's correlation analysis and the backward stepwise multiple regression analysis were performed. FEV1 , FVC, PEF and FEV1 /FVC results were compared to the NHANES III African American predicted values as well as to the GLI2012 equations. FEV1 measurements are lower than the reference values according to NHANES III and GLI2012 equations by 22·1% and 25·8%. FVC results fell short of the NHANES III predicted by 29·5% and of GLI2012 by 32·5%. The average %FEV1 /FVC scores for the boys and men exceeded the recommended GLI2012 predicted by 10·5-15·2%. All the spirometric measurements included in the analysis were statistically significantly correlated with age, body height, sitting height, trunk length and body weight. The application of prediction formulae developed for non-African populations overestimates the values for Black Africans. The results of spirometric measurements are ecosensitive and dependent on various external (environmental) factors.

Macro- and Microelement Content and Other Properties of Chaenomeles japonica L. Fruit and Protective Effects of Its Aqueous Extract on Hepatocyte Metabolism.

This growing interest in the cultivation of Japanese quince Chaenomeles japonica L. results from the potentially beneficial properties of its fruit. Fresh fruits are very firm and too acidic to eat raw, but their bioactive components, distinctive aroma, and high amount of dietary fiber make the fruits well suited for industrial processing. However, not all the properties of the fruit have been investigated. For example, there are no comprehensive reports about the mineral content or potentially harmful effects on liver metabolism. Hence, the purpose of our study was to examine fresh Japanese quince fruit in terms of (1) ascorbic acid, oxalate, fiber, macro- and micronutrients, dry matter, extract, total acidity, antioxidant activity, and phenolic compound levels; and (2) the effect of its extract on in vitro hepatocyte metabolism, measured by the concentration of lipid peroxides (LPO) and reactive oxygen species (ROS) and the severity of apoptosis and necrosis. The fruit of C. japonica had high levels of macro- and microelements, ascorbic acid, phenolic compounds, fiber, and low oxalate levels. Our analysis of macro- and microelements showed that the average content of Fe was 0.516 mg/g, Cu 0.146 mg/g, Zn 0.546 mg/g, Mg 16.729 mg/g, and Ca 22.920 mg/g of fresh fruit. A characteristic feature of the fresh fruit of C. japonica is a high level of polyphenols, which-combined with a high content of vitamin C-affect their high antioxidant potential. In the tested hepatocyte cultures incubated with extract of the Japanese quince, we observed a significant decrease in the concentration of lipid peroxides compared to the control. There were also no signs of increased formation of ROS in the mitochondria of hepatocytes incubated with the extract of quince. Malondialdehyde was strongly negatively correlated with the concentration of Japanese quince extract, which indicates the hepatoprotective properties of Japanese quince. In addition, our analysis of confocal microscopy images showed that the hepatocytes incubated with the extract of Japanese quince at any concentration did not show any signs of apoptosis or necrosis. The aqueous extract of quince fruit has antioxidative and antiapoptotic hepatocytes, thus exerting a hepatoprotective effect.

Cadmium Concentration in Mother's Blood, Milk, and Newborn's Blood and Its Correlation with Fatty Acids, Anthropometric Characteristics, and Mother's Smoking Status.

The aim of this study was to determine cadmium concentration in mothers' blood, milk, and newborns' blood from Szczecin (Poland) as a result of environmental cadmium exposure and evaluate the correlation (1) between cadmium levels in analyzed matrices, (2) between cadmium and fatty acids in those matrices, and (3) between cadmium and some selected personal variables, such as anthropometric characteristics, mothers' smoking status, and fruit and fish consumption by mothers. The concentration of cadmium in whole blood and milk of mothers and in the umbilical cord blood of newborns was determined by atomic absorption spectrometry with graphite furnace atomization and Zeeman correction. The fatty acid concentrations were determined by gas chromatography in our previous study. The mean concentrations of cadmium in maternal blood, newborn's blood, and breast milk were 0.61 ± 0.62 µg/L, 0.05 ± 0.04 µg/L, and 0.11 ± 0.07 µg/L, respectively, and differed significantly between analyzed matrices. Cadmium concentrations in the umbilical cord blood were 15 % (range 0-83 %) of the concentration in maternal blood, whereas cadmium concentrations in breast milk constituted 35 % (range 3-142 %) of the concentration in mothers' blood. No correlation was found between cadmium levels in three analyzed matrices. The correlation analysis revealed significant low positive correlation between maternal blood cadmium concentrations and concentrations of elaidic, oleic, and cis-vaccenic acids in mothers' milk (correlation coefficients 0.30, 0.32, and 0.31, respectively). Mothers' blood cadmium correlated with mothers' age (r = -0.26, p = 0.03), maternal smoking before pregnancy (r = 0.55, p < 0.000), maternal smoking during pregnancy (r = 0.58, p < 0.000), and fruit consumption by mothers after delivery (r = -0.44, p = 0.003). Mothers' height was the only variable that correlated significantly with breast milk cadmium levels. Newborns' blood cadmium concentrations correlated significantly with mothers' height (r = 0.28, p = 0.02), newborns' birth weight (r = 0.26, p = 0.03), maternal smoking during pregnancy (r = 0.24, p = 0.048), and fish consumption by mothers after delivery (r = 0.37, p = 0.02). The concentrations of cadmium in Polish mother-newborn pairs are among the lowest in Europe and within the norms established by different institutions. The results of our study confirm the existence of effective partial barriers (such as the placenta and mammary gland) restricting cadmium passage from mother to newborn. The significant positive correlations between maternal blood Cd and concentrations of oleic, elaidic, and cis-vaccenic acids in breast milk might suggest the increased cadmium toxicity to infant, taking into consideration even low cadmium passage to milk. Maternal smoking during pregnancy increases both maternal and newborn's blood cadmium level. Promotion of nonsmoking among pregnant women could substantially reduce prenatal and neonatal exposure to cadmium. Moreover, the results of our study point to the need of establishing complex biomonitoring of cadmium in mother-infant pairs in order to better protect children from this toxic and carcinogenic metal exposure.

Environmental Lead (Pb) Exposure Versus Fatty Acid Content in Blood and Milk of the Mother and in the Blood of Newborn Children.

Significant progress in understanding the effects of the neurotoxic action of lead (Pb) in young organisms had led to reduction of "safe" level in the blood (Pb-B) to 5 µg/dL in children and pregnant women. Prolonged exposure to relatively low levels of Pb, generally asymptomatic and subclinical (i.e., microintoxication), is currently the dominant form of environmental poisoning, and its negative effects on health may appear after many years, e.g., secondary contamination from Pb bone deposits released in pregnancy. Therefore, the aim of this study was to investigate the effect of environmental exposure (urban areas) of mothers to Pb, on its levels in their milk and blood and in the blood of newborns. Moreover, the aim was to determine the fatty acid profile in the mothers' blood and milk and in the blood of newborns. We also wanted to find if infant birth weight depends on Pb blood levels, as well as on Pb and fatty acid levels in the blood and milk of the mothers. Finally, we examined if the mothers' weight and body mass index (BMI) before pregnancy influenced the concentration of Pb and fatty acid profile in the blood and milk of mothers and in the blood of their children. Analysis of fatty acids elaidic (C18:1, 9t), oleic (C18:1, 9c), vaccenic (C18:1, 11t), cis-vaccenic (C18:1, 11c), linoleic (C18:2, cis), γ-linolenic (C18:3, n-6), α-linolenic (C18:3, n-3), arachidonic (C20:4, n-6), eicosapentaenoic (C20:5, n-3), and docosahexaenoic (C22:6, n-3) was conducted by gas chromatography. The concentration of Pb in the whole blood and milk were determined by atomic absorption spectrometry with graphite furnace atomization and Zeeman correction. Our study established a significant and strong correlation between the content of Pb in the blood of the mother and the child. This supports the assumption that the transport of Pb through the placenta is neither regulated nor selective. Environmental maternal exposure to lead resulting in Pb-B levels considered safe for pregnant women had no effect on infant birth weight, the concentration of fatty acids in the blood and milk of mothers, or in the blood of newborns. Mothers' weight and BMI before pregnancy had no effect on the concentration of Pb and studied fatty acid profile.

Selected anthropometric indices of maritime university students.

BACKGROUND: Overweight and obesity during working life are becoming an increasingly serious challenge to various professional groups where recruits and personnel must be healthy and fit. Marine recruitment, even at the training stage, should be open to applicants who meet health and fitness criteria. The objective of the study is to determine the overweight and adiposity rates among seafarer candidates (n = 368). Based on anthropometric measurements and somatic indices the extent of obesity among marine students/ /future seafarers was investigated. MATERIALS AND METHODS: In the groups identified according to the year of study, arithmetic averages (SD - standard deviation) were calculated for somatometric characteristics, and were then used to analyse the phenomena of overweight and obesity. The comparison was performed using the Kruskal-Wallis test, one-way analysis of variance (ANOVA) by ranks. RESULTS: The highest average body mass index (BMI) score was found in fourth-year students (mean BMI 25.7 ± 2.8). The average BMI for years one and two was in the upper range of 'healthy' weight. In 24.0% of first-year students and 32.2% of second-year students, the waist circumference was higher than half of the body height. Body fat percentage results indicate that this feature is highly variable, with a strong upward trend. CONCLUSIONS: Findings regarding overweight among future seamen give cause for concern. The participants of the study were characterised by excessive weight and adiposity. Recruitment criteria for uniformed services are not as restrictive as they used to be, as it is getting increasingly more difficult to find sufficiently slim and fit applicants.

Dependence between Age at Menarche, Body Composition and Selected Somatic Indices.

The aim of this paper is to analyse age at menarche in Tanzanian girls for associations with body composition and selected somatic indices. The study included 97 girls (71 post-menarche and 26 pre-menarche). Measurements were taken of body height, body weight, waist and hip circumferences. Body composition was analysed for percentages of body H2O, fat, and muscle. The girls' nutritional status and body fat distribution were assessed using the following somatic indices: BMI, WHR (Waist-to-Hip Ratio) and WHtR (Waist-to-Height Ratio). For the post-menarcheal group N=71, backward stepwise multiple regression analysis was performed. Average age at menarche calculated using the recall method for N=71 was X=14.29 years, calendar age X=15.92 years. More than half (57.69%) of the girls in the pre-menarche group had not reached the body weight of 48kg, whereas in the post-menarche group they accounted for 32.39%. As many as 97.18% of the post-menarche girls had body fat content of 22%, compared to 88.46% in the pre-menarche group. In terms of BMI, 74.65% of post-menarche girls had healthy weight, compared to 73.08% in the pre-menarche group. There were no significant differences in WHR or WHtR, which may indicate similar fat distribution in girls prior to and after menarche. The analysis showed that the onset of menstruation is not strictly dependent on reaching a critical point in body weight or body composition.

Spirometry results (FEV1 and FVC) in young Bantu men from Tanzania vs environmental and family characteristics.

OBJECTIVES: The aim of this paper was to examine the extent to which socioeconomic factors, anthropological data and somatic indices influenced the results of spirometric measurements (FEV1 and FVC) in Tanzanian youth. MATERIALS AND METHODS: The population studied were young black Bantu men aged 12.8-24.0 years. Analysis was performed for the whole data set (n = 255), as well as separately for two age groups: under 17.5 years (n = 168) and 17.5 + (n = 87). A backward stepwise multiple regression analysis was performed for FEV1 and FVC as dependent variables on socioeconomic and anthropometric data. RESULTS: Multiple regression analysis for the whole group revealed that the socioeconomic and anthropometric data under analysis accounted for 38% of the variation in FEV1. In addition the analysis demonstrated that 34% of the variation in FVC could be accounted for by the variables used in the regression. A significant impact in explaining the variability of FVC was exhibited by the thorax mobility, financial situation of the participants and Pignet-Verwaecka Index. CONCLUSION: Analysis of the data indicates the significant role of selected socio-economic factors on the development of the biological specimens investigated. There were no perceptible pathologies, and the results can be treated as a credible interpretation of the influence exerted by the environment in which the teenagers under study grew up.

The content of fluoride, calcium and magnesium in the hair of young men of the Bantu language group from Tanzania versus social conditioning.

The present study aimed at analysing the content of fluorine (F), calcium (Ca) and magnesium (Mg) in the hair of young male students (n =52) of a secondary school in Mafinga in Tanzania (Africa) who participated in anthropological examinations. Ca and Mg concentrations were determined using atomic absorption spectrophotometer while F levels using a potentiometric method. F in the hair of boys from older group (≥16 years old; n =24) was significantly higher than in the younger group (<16 years old; n =28) versus Ca and Mg levels. High carbohydrate diet was predominant- mainly based on corn or bean and meat served once a week, with few fruit and raw vegetables. Collective catering in the dormitory reflected habits and culinary preferences at home. The lack of balanced diet, with majority of the nutritional energy supplied by easily accessible and cheap carbohydrates, was reflected in dietary deficiencies, characterised, among others, by visible skin conditions and tooth decay.

Changes in the body build of students of the Maritime University of Szczecin in the years 1969-2007.

BACKGROUND: Changes in the human body build over the last century refer to greater and greater body heightbut also to the "plague" of obesity in the period of the last 30 years. The secular trend and excessiveweight gain being observed in societies of rich and developing countries is global in nature. Students ofacademies that traditionally prepare for working at sea on ships have been required until recently to be ofvery good health and physical fitness. Changes in the body build, particularly obesity, may interfere withthe selection of candidates for the profession of seaman in the near future. AIM: The aim of this study is to characterise the body build of students of the Maritime University of Szczecin.The results of the author's own research from 2006 and 2007 were compared with those of similaranthropometric studies being published in the literature from previous years (1969-2007). MATERIALS AND METHODS: The author's own research material consisted of a group of students of the MaritimeUniversity of Szczecin (n = 171). Anthropometric measurements (body height, body weight, andwaist, hip, and arm circumferences) were made in 2006 and 2007. Based on these anthropometric data,the body mass index and the Rohrer's index were calculated. Arithmetic means, standard deviations, andrange of variation (min-max) were calculated for all anthropometric measurements and somatic indicesand compared with the results from previous years (1969-2007). RESULTS AND CONCLUSIONS: During the analysed years 1969-2007, the examined students of the MaritimeUniversity of Szczecin showed increasing body height and, worryingly, rising body weight and waist andhip circumferences. This is evidence of visceral fatness, which is dangerous for health and increases therisk of cardiovascular diseases. One of the reasons for the changes in the body build is decreasing motoractivity. The increases in the traits being analysed were not steady over time; larger increases in bodyheight occurred in the years of the more stable socio-economic situation in Poland.

Blood pressure and levels of Fe, Ca, Mg, Zn, Cu, Na and K in the hair of young Bantu men from Tanzania.

Mineral imbalance in the body may significantly contribute to the development and course of hypertension. In this paper, blood pressure figures have been linked to the levels of Fe, Ca, Mg, Zn, Cu, Na and K in hair. The research sample was composed of young men (n = 91) aged 13-21, from the town of Mafinga, Iringa District, Tanzania. The data collected included their age, tribal background and weekly diet. Based on body mass index, the participants were categorised into pre-defined subgroups. To examine how the minerals in question affect blood pressure, correlation analysis and multiple ridge regression analysis were performed. Analysis of ridge regression findings for the researched group (n = 91) shows that the minerals under scrutiny account for systolic blood pressure variation in 13 % and in 15 % for diastolic blood pressure variation. After including two additional variables-calendar age and body mass index-in regression analysis, the ultimate coefficient of determination (R (2)) changes for systolic blood pressure and remains the same for diastolic blood pressure (R (2) = 0.194 and R (2) = 0.156, respectively). Nutritional analysis shows that the students included in the study received insufficient calories per day (1,500-2,200 kcal). The group of students with abnormal blood pressure were not aware of their poor health. Research findings may result from progressive environmental changes and poor nutrition in terms of food quantity and quality, which had an impact on the subjects' blood pressure. Hair analysis used to determine mineral content in the body may be an auxiliary tool in identifying the links between factors leading to the development of hypertension.