RESUMO
BACKGROUND: Chemokines are pro-inflammatory cells that can be induced during an immune response to recruit cells of the immune system to a site of infection. AIM: This study was conducted to detect the presence of chemokines, macrophage inflammatory protein-1α (MIP-1α), and 1ß (MIP-1ß) and estimate their levels in gingival crevicular fluid (GCF) in children with band and loop space maintainers. MATERIALS AND METHODS: MIP-1α and MIP-1ß levels were estimated in GCF samples from twenty healthy children and twenty children with band and loop space maintainers. Periodontal status was evaluated by measuring gingival index, plaque index, and Russell's periodontal index. The GCF samples were quantified by ELISA, and the levels of MIP-1α and MIP-1ß were determined. RESULTS: The mean MIP-1α concentrations in healthy children and those with space maintainers were 395.75 pg/µl and 857.85 pg/µl, respectively, and MIP-1ß was 342.55 pg/µl and 685.25 pg/µl, respectively. MIP-1α and MIP-1ß levels in GCF from children with space maintainers were significantly higher than in the healthy group, and statistically significant difference existed between these two groups. CONCLUSION: MIP-1α and MIP-1ß can be considered as novel biomarkers in the biological mechanism underlying the pathogenesis of gingival inflammation in children with space maintainers.
RESUMO
AIMS AND OBJECTIVES: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1ß and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. MATERIALS AND METHODS: A total of 80 children with primary dentition were selected and categorized into four groups with twenty in each group; Group 1 - healthy subjects, Group 2 - dental caries, Group 3 - dental caries involving the pulp, and Group 4 - stainless steel crowns. GCF samples were collected by an extra-crevicular method with microcapillary pipettes. The GCF samples were quantified by ELISA and the levels of MIP-1α and MIP-1ß were determined. RESULTS: MIP-1α and MIP-1ß were detected in all the samples. Highest mean concentration in GCF was obtained for Group 3 followed by Groups 2 and 4 while the lowest concentration was seen in Group 1. This suggests that MIP-1α and MIP-1ß levels in GCF increased proportionately with the inflammation. CONCLUSIONS: GCF serves as a noninvasive diagnostic fluid to measure biomarkers released during dental caries initiation and progression. MIP-1α and MIP-1ß chemokines can be considered as novel biomarkers, in biological mechanism underlying the pathogenesis and inflammation in children with dental caries and stainless steel crowns.
