A novel type of meso-diaminopimelic acid-based peptidoglycan and novel poly(erythritol phosphate) teichoic acids in cell walls of two coryneform isolates from the surface flora of French cooked cheeses.

The primary structure of the peptidoglycan and the teichoic acids of two coryneform isolates from the surface flora of French cooked cheeses, CNRZ 925 and CNRZ 926, have been determined. In the peptidoglycan, meso-diaminopimelic acid was localized in position three of the peptide subunit. It contained an D-glutamyl-D-aspartyl interpeptide bridge, connecting meso-diaminopimelic acid and D-alanine residues of adjacent peptide subunits. The alpha-carboxyl group of D-glutamic acid in position two of peptide subunits was substituted with glycine amide. The teichoic acid pattern and composition differed between the strains: both contained an erythritol teichoic acid and strain CNRZ 925 also contained an N-acetylglucosaminylphosphate polymer. The erythritol teichoic acids differed in terms of the quality and quantity of substituents, but they both had N,N'-diacetyl-2,3-diamino-2,3-dideoxyglucuronic acid in common.

Biological effect-test systems for the early recognition of unexpected environmental changes.

Pre-marketing assessments of chemicals regarding their potential environmental impacts contain numerous sources of error and uncertainties. Practices and methods for the evaluation of environmental risks posed by individual pre-selected agents seem unsuitable for detecting unexpected trends in the environment. This is particularly so in view of the multitude of anthropogenic assaults on the environment and their complex interactions. Therefore, requirements for symptom-oriented biological effect-test systems, 'bioprobes', capable of reacting sensitively to environmental changes of unknown origin were conceived. Bioprobes designed to detect biological effects should respond to a broad spectrum of anthropogenic chemicals. They are environmental quality measuring devices consisting of cultivated organisms, cells, organelles or biomolecules in combination with recording units. In contrast to traditional biotests for chemical testing, bioprobes are deliberately exposed to selected segments of the environment for certain periods of time. During and after such exposures, changes of defined biological states or process rates-'observation elements'-are registered.

A concept for detecting unexpected changes in the environment early.

A project was established under the auspices of the German Federal Ministry of Research and Technology with the task of developing a concept for the early recognition of signals for unexpected deleterious changes in the environment. Predictive assessments of products (e.g., chemicals) and technologies as regards their potential environmental impacts contain numerous sources of error and uncertainties. Therefore, appropriate observation systems are needed in order to detect unexpected developments in the environment and in human health. Long-term storage of samples (environmental specimen banking) is the third basic activity that must be undertaken within an early warning system. When monitoring the environment for unwanted changes, it is not sufficient to trace some individual agents, preselected for their well-known capacity to inflict harm. The following avenues of identifying symptoms of change in the environment were identified: development and deployment of "key indicators"; acquisition and correlative treatment of conventional monitoring data from different sources; and execution of concise studies in environmental problem areas. Key indicators are defined as scientific measurement and observation methods which respond to early stages of anthropogenically induced environmental changes and are capable of responding to a multitude of simultaneously present agents. A series of examples is discussed. Approaches concerning how the statistical evaluation of available sets of environment-related data could reveal unexpected developments are presented.