Big data analytics and artificial intelligence aspects for privacy and security concerns for demand response modelling in smart grid: A futuristic approach.

Next generation electrical grid considered as Smart Grid has completely embarked a journey in the present electricity era. This creates a dominant need of machine learning approaches for security aspects at the larger scale for the electrical grid. The need of connectivity and complete communication in the system uses a large amount of data where the involvement of machine learning models with proper frameworks are required. This massive amount of data can be handled by various process of machine learning models by selecting appropriate set of consumers to respond in accordance with demand response modelling, learning the different attributes of the consumers, dynamic pricing schemes, various load forecasting and also data acquisition process with more cost effectiveness. In connected to this process, considering complex smart grid security and privacy based methods becomes a major aspect and there can be potential cyber threats for the consumers and also utility data. The security concerns related to machine learning model exhibits a key factor based on different machine learning algorithms used and needed for the energy application at a future perspective. This work exhibits as a detailed analysis with machine learning models which are considered as cyber physical system model with smart grid. This work also gives a clear understanding towards the potential advantages, limitations of the algorithms in a security aspect and outlines future direction in this very important area and fast-growing approach.

Advanced Driver Assistance System Based on IoT V2V and V2I for Vision Enabled Lane Changing with Futuristic Drivability.

In conventional modern vehicles, the Internet of Things-based automotive embedded systems are used to collect various data from real-time sensors and store it in the cloud platform to perform visualization and analytics. The proposed work is to implement computer vision-aided vehicle intercommunication V2V (vehicle-to-vehicle) implemented using the Internet of Things for an autonomous vehicle. Computer vision-based driver assistance supports the vehicle to perform efficiently in critical transitions such as lane change or collision avoidance during the autonomous driving mode. In addition to this, the main work emphasizes observing multiple parameters of the In-Vehicle system such as speed, distance covered, idle time, and fuel economy by the electronic control unit are evaluated in this process. Electronic control unit through brake control module, powertrain control module, transmission control module, suspension control module, and battery management system helps to predict the nature of drive-in different terrains and also can suggest effective custom driving modes for advanced driver assistance systems. These features are implemented with the help of the vehicle-to-infrastructure protocol, which collects data through gateway nodes that can be visualized in the IoT data frame. The proposed work involves the process of analyzing and visualizing the driver-influencing factors of a modern vehicle that is in connection with the IoT cloud platform. The custom drive mode suggestion and improvisation had been completed with help of computational analytics that leads to the deployment of an over-the-air update to the vehicle embedded system upgradation for betterment in drivability. These operations are progressed through a cloud server which is the prime factor proposed in this work.

An IoT-Based Wristband for Automatic People Tracking, Contact Tracing and Geofencing for COVID-19.

The coronavirus disease (COVID-19) pandemic has triggered a huge transformation in the use of existing technologies. Many innovations have been made in the field of contact tracing and tracking. However, studies have shown that there is no holistic system that integrates the overall process from data collection to the proper analysis of the data and actions corresponding to the results. It is critical to identify any contact with infected people and to ensure that they do not interact with others. In this research, we propose an IoT-based system that provides automatic tracking and contact tracing of people using radio frequency identification (RFID) and a global positioning system (GPS)-enabled wristband. Additionally, the proposed system defines virtual boundaries for individuals using geofencing technology to effectively monitor and keep track of infected people. Furthermore, the developed system offers robust and modular data collection, authentication through a fingerprint scanner, and real-time database management, and it communicates the health status of the individuals to appropriate authorities. The validation results prove that the proposed system identifies infected people and curbs the spread of the virus inside organizations and workplaces.

Correlation of intestinal structure and function in HIV infection.

The gastrointestinal tract has great importance in HIV infection because of its role as a primary barrier to the external environment and consequent need for effective immune function. Many factors promote the development of diarrhea in HIV-infected individuals. Understanding the genesis of the symptom is key to formulating effective therapy. Ultimate control of the problem depends on preventing HIV replication and immune depletion, as well as avoiding the development of opportunistic enteric infections in patients with severe immune deficiency.

Selective depletion of rectal lamina propria rather than lymphoid aggregate CD4 lymphocytes in HIV infection.

The goal of this study was to examine the changes in lymphocyte populations in rectal mucosa during HIV infection and to study their relationship to mucosal immunity and to systemic depletion of CD4 lymphocytes. Rectal biopsies from 58 HIV-infected subjects and eight controls were studied. Frozen rectal tissue sections were stained with antibodies to CD4, CD3, CD8, and markers for macrophages. HIV-infected subjects were divided into early stage (no opportunistic infections) and AIDS groups. There was profound depletion of rectal lamina propria CD4 lymphocytes (16% and 6% of normal content in early and AIDS groups, respectively). However, lymphoid aggregate CD4 lymphocytes were far less severely depleted (69% and 40% of normal content, respectively). The extent of lymphoid aggregate CD4 lymphocyte depletion generally parallelled the CD4 lymphocyte depletion in the blood. CD8 lymphocyte content in both the lamina propria and lymphoid aggregates usually were increased, particularly in early-stage patients. Macrophage contents were usually normal in the HIV-infected groups. We conclude that rectal lamina propria and lymphoid aggregates are distinct compartments differing markedly in their CD4 lymphocyte content during HIV infection. In light of this and an increased number of apoptotic cells which were noted in rectal lamina propria in HIV-infected subjects, we hypothesize that intestinal lamina propria could be a site of rapid CD4 lymphocyte destruction during HIV infection.

Variation in the expression of human immunodeficiency virus RNA and cytokine mRNA in rectal mucosa during the progression of infection.

Previous studies demonstrated that mucosal HIV p24 antigen content varied during the progression of HIV infection. In this study, expression of HIV RNA and mRNA of selected cytokines was examined in rectal mucosa from HIV-infected individuals. Rectal biopsies from 27 subjects were studied: 7 with CD4 counts > 500/mm3 (early), 11 with CD4 < 500 (intermediate), and 9 with AIDS (late), plus 4 HIV-seronegative controls. RNA in situ hybridization was performed using 35S-labeled riboprobes of HIV, TNF-alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-10, INF-alpha, IFN-gamma, and TGF-beta. HIV RNA was detected more frequently in the intermediate group than in the other groups (p < 0.005). Cytokine mRNA expression also varied during disease progression. The expression of IFN-alpha, IFN-gamma, and TGF-beta mRNA was most prevalent early in the disease; peak expression of IL-4, IL-5, IL-6, and IL-10 was seen during the intermediate stage, and peak expression of TNF-alpha and IL-1 beta mRNA were seen in AIDS patients. HIV RNA and cytokine mRNA expression vary during HIV disease progression. HIV RNA expression is greatest in the intermediate stage of the disease. The pattern of cytokine mRNA expression suggests predominant cell-mediated immunity under basal conditions and early in the disease, generalized cytokine activation in its middle phase, and proinflammatory cytokine activation in AIDS patients. Cytokine modulation of HIV expression in rectal mucosa in vivo may occur and have pathogenic importance.

Detection and localization of HIV RNA and TNF mRNA in rectal biopsies from patients with AIDS.

The objective of this study was to compare the localization of cells containing human immunodeficiency virus (HIV) RNA and tumor necrosis factor (TNF)-alpha mRNA in rectal mucosa by RNA in-situ hybridization in a retrospective analysis of archived rectal biopsy specimens. RNA in-situ hybridization studies were performed in 27 HIV-seropositive individuals and seven controls, using antisense and sense 35S-labeled riboprobes. The detection and localization of positive cells were compared. HIV was RNA detected in 44% of biopsies, while TNF-alpha mRNA detected in 22%. TNF mRNA was found in biopsies from patients with and without opportunistic infections. All cells expressing TNF-alpha mRNA and most of the cells expressing HIV RNA were found in close proximity to the epithelial surface. The content of an HIV-associated protein, p24, in mucosal homogenates, determined by a quantitative ELISA technique was significantly higher in the subgroup of patients with positive in situ hybridization studies for TNF-alpha mRNA than in the subgroup with negative studies. The colocalization of TNF-alpha mRNA and HIV RNA immediately beneath the epithelium suggests a specific relationship between them, as well as a possible relationship to a luminal factor.

Intestinal mucosal inflammation associated with human immunodeficiency virus infection.

The role of the human immunodeficiency virus type-1 (HIV) in producing intestinal disease was studied prospectively in 74 HIV-infected individuals with (43) or without (31) the acquired immunodeficiency syndrome (AIDS). Thirty-one subjects had enteric infections; all but one had AIDS. Alteration in bowel habits was the most common symptom and occurred independently of enteric infections. Abnormal histopathology was present in 69% of cases, and the finding was associated with altered bowel habits. An HIV-associated protein, p24, was detected in 71% of biopsies by ELISA assay. Tissue p24 contents varied with disease stage and were highest in HIV-infected individuals without AIDS (Walter Reed classes 3 and 4). Tissue p24 detection was associated with both altered bowel habits and histologic mucosal abnormalities. Tissue contents of the cytokines, tumor necrosis factor-alpha and interleukin-1 beta, were higher in HIV-infected individuals than in controls and their elevations were independent of enteric infection. We conclude that HIV reactivation in the intestinal mucosa may be associated with an inflammatory bowel syndrome in the absence of other enteric pathogens.

Effects of enteric parasitoses and HIV infection upon small intestinal structure and function in patients with AIDS.

We compared, retrospectively, the effects of infection in jejunal mucosa with the protozoa cryptosporidia or microsporidia and with the human immunodeficiency virus (HIV) upon mucosal structure and absorptive function in 29 AIDS patients. The presence or absence of protozoal infection was confirmed by transmission electron microscopy. Villus blunting and crypt hyperplasia were seen mainly in the parasite-infected groups, although two patients without parasites also had shortened villi. Absorptive functions, including disaccharidase-specific activities and D-xylose absorption, closely paralleled the degree of small intestinal alteration. Evidence of HIV-infected cells in jejunal mucosa was examined by RNA in situ hybridization and by antigen-capture ELISA of mucosal homogenates. We found evidence of HIV in almost half the patients, which did not correlate with intestinal injury or diminished absorption.

Chronic idiopathic esophageal ulceration in the acquired immunodeficiency syndrome. Characterization and treatment with corticosteroids.

Study objectives were to characterize the clinical syndrome of chronic idiopathic esophageal ulceration in patients with acquired immunodeficiency syndrome (AIDS), to determine the extent of local human immunodeficiency virus (HIV) infection, and to evaluate the effect of corticosteroid therapy upon symptoms and healing. Twelve AIDS patients with chronic esophageal ulcers whose etiology remained unknown after clinical evaluation were the subjects. All patients complained of severe odynophagia, chest pain, and weight loss. Barium radiography and endoscopy demonstrated large, undermined ulcers with severe acute inflammation. No evidence of herpes simplex viruses I or II, cytomegalovirus, fungi, or tumors were found histologically. Evidence of HIV was found in all ulcers using a combination of RNA in situ hybridization, immunohistochemistry, and quantitative antigen capture enzyme-linked immunosorbent assay of tissue homogenates. Steroid therapy by the oral or intravenous routes or by direct intralesional injection resulted in pain relief, weight gain in 10 patients, and ulcer healing in five patients. A characteristic clinical syndrome of chronic idiopathic esophageal ulceration may occur in patients with AIDS, related to local HIV infection in the esophagus. Corticosteroids relieve symptoms and may promote healing of the ulcer.

Rectal mucosal pathology varies with human immunodeficiency virus antigen content and disease stage.

Rectal mucosal biopsy specimens from 75 human immunodeficiency virus (HIV)-seropositive and 16 HIV-seronegative subjects were examined. The histopathologic changes were correlated with immunoperoxidase staining for UCHL-1 and HIV core protein p24, quantitative p24 enzyme-linked immunosorbent assay (ELISA) assay in homogenized rectal tissue and serum, and a modified Walter Reed clinical stage. Four phases were seen in the HIV-infected subjects: (1) early phase, in Walter Reed stage 1-2 subjects, with nearly normal histology and low p24; (2) inflammatory phase, typically in Walter Reed stage 3-4 subjects, with a superficial lamina propria infiltrate of lymphocytes, plasma cells, and eosinophils with degranulation, abundant UCHL-1 staining, and maximal p24 by both immunoperoxidase staining and ELISA; (3) transitional phase, in many Walter Reed 5 and some Walter Reed 6 subjects, with normal lymphocyte population density but with subtle inflammatory changes; and (4) lymphoid depletion phase, mainly in Walter Reed stage 6 subjects, with decreased lymphocytes but often with endothelial cell activation and apoptosis. These phases presumably result from effective HIV suppression by a relatively intact immune system, followed by maximal HIV infection and lymphocyte activation, then progressive lymphocyte depletion. The inflammation correlated with the presence and amount of HIV in rectal tissue determined by immunohistochemistry and ELISA and was maximal before overt immunodeficiency developed. Intestinal mucosa could be a preferred site of HIV proliferation and T-cell destruction.

Detection, localization, and quantitation of HIV-associated antigens in intestinal biopsies from patients with HIV.

This study determined the prevalence, cellular localization, and content of human immunodeficiency virus (HIV)-associated antigens in intestinal mucosa from HIV-infected subjects. Studies were performed in 168 subjects with gastrointestinal, nutritional, or proctologic complaints, and HIV-seronegative controls. The polymerase chain-reaction technique, which detects viral DNA, was used in 20 subjects and was positive in 70%. In situ hybridization studies, using RNA probes, were employed in 48 cases and were positive in 31%. Immunohistologic studies using monoclonal antibodies to HIV p24 antigen were employed in 73 cases and were positive in 67%. Quantitative ELISA assays for tissue p24 content were performed in 168 cases and was positive in 68%. Evidence of HIV was found throughout the intestine and in different disease stages. The quantitative ELISA studies correlated significantly with in situ hybridization, implying a possible association between the presence of viral RNA and protein expression. The authors conclude that HIV is present in intestinal mucosa from most, if not all HIV-infected subjects. The relationship to intestinal disease currently is unclear.