RESUMO
OBJECTIVE: To investigate the mechanism of Cr-induced DNA damage in glouse. METHODS: The studies were conducted at three pH values(pH 6.5, 7.5, 8.5) with a range of sugars, including D-glucose, alpha-methyl-D-glucose, 2-deoxy-D-glucose, and D-glucose-C-d7. DNA damage was analyzed by gel electrophoresis, and the new species and their lifetime were detected by EPR. RESULTS: The results of these experiments have reinforced the postulate that Cr (V) complexes are likely to be involved in the genotoxic damage that eventually results in Cr-induced cancers. With an increase in the pH values, the amount of Form II DNA decreased due to the increasing rate of Cr (V) disproportionation and H concentration. On the other hand, the presence of sugars in the reaction system generated Cr-mixed-ligand complexes, which could also cause DNA cleavage. CONCLUSION: Proposed mechanism of DNA cleavage are presented induced by Cr (V)-mixed-ligand.
