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1.
J Hosp Infect ; 104(4): 503-507, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31874205

RESUMO

The duration of eXDR carriage depends on several factors that might be difficult to recover. We aim to assess the duration of eXDR carriage by using a simple to recover parameter: the number of consecutive negative screening. 131 eXDR carriers (51 VRE and 80 CPE) were included. The number of consecutive negative screenings was strongly associated with eXDR clearance. All patients displaying at least three negative screenings over a seven-month period were never screened positive thereafter. Taking into account the number of negative screenings as a part of a case-by-case risk assessment would be helpful for the decision to maintain or lift eXDR-focused precautions.


Assuntos
Enterobacteriáceas Resistentes a Carbapenêmicos/isolamento & purificação , Portador Sadio/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Enterococos Resistentes à Vancomicina/isolamento & purificação , Fezes/microbiologia , Humanos , Laboratórios Hospitalares , Paris
3.
Genome Announc ; 2(4)2014 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-25013134

RESUMO

The attenuated BCG strain of Mycobacterium bovis is widely used as a vaccine against tuberculosis. However, in rare cases, it can be pathogenic to humans. Here, we report the first draft of a whole-genome sequence of a multidrug-resistant clinical isolate of M. bovis BCG.

4.
Euro Surveill ; 18(33): 20555, 2013 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-23968874

RESUMO

A marked increase in the number of multidrug-resistant (MDR) tuberculosis (TB) cases entirely related to patients born in the Former Soviet Union was observed in France in the last two years. Very few cases were clustered, suggesting it is a consequence of recent immigration of patients already infected in their country of origin. This major increase challenges the existing structures for management of MDR and extensively drug-resistant TB (XDR-TB).


Assuntos
Tuberculose Extensivamente Resistente a Medicamentos/diagnóstico , Tuberculose Extensivamente Resistente a Medicamentos/etnologia , Mycobacterium tuberculosis/isolamento & purificação , Pacientes/estatística & dados numéricos , Tuberculose Resistente a Múltiplos Medicamentos/diagnóstico , Tuberculose Resistente a Múltiplos Medicamentos/etnologia , Adulto , Antituberculosos/uso terapêutico , Tuberculose Extensivamente Resistente a Medicamentos/tratamento farmacológico , Feminino , França/epidemiologia , Genótipo , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/efeitos dos fármacos , Mycobacterium tuberculosis/genética , Fatores de Risco , Sequências de Repetição em Tandem , Tuberculose Resistente a Múltiplos Medicamentos/tratamento farmacológico , U.R.S.S./etnologia , Adulto Jovem
5.
Med Mal Infect ; 43(8): 322-30, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23876208

RESUMO

PCR targeting the gene encoding 16S ribosomal RNA (commonly named broad-range PCR or 16S PCR) has been used for 20 years as a polyvalent tool to study prokaryotes. Broad-range PCR was first used as a taxonomic tool, then in clinical microbiology. We will describe the use of broad-range PCR in clinical microbiology. The first application was identification of bacterial strains obtained by culture but whose phenotypic or proteomic identification remained difficult or impossible. This changed bacterial taxonomy and allowed discovering many new species. The second application of broad-range PCR in clinical microbiology is the detection of bacterial DNA from clinical samples; we will review the clinical settings in which the technique proved useful (such as endocarditis) and those in which it did not (such as characterization of bacteria in ascites, in cirrhotic patients). This technique allowed identifying the etiological agents for several diseases, such as Whipple disease. This review is a synthesis of data concerning the applications, assets, and drawbacks of broad-range PCR in clinical microbiology.


Assuntos
Bacteriologia/tendências , Reação em Cadeia da Polimerase/métodos , Ribotipagem/métodos , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Infecções Bacterianas/diagnóstico , Infecções Bacterianas/microbiologia , DNA Bacteriano/genética , DNA Ribossômico/genética , Reações Falso-Positivas , Previsões , Humanos , Espectrometria de Massas , Reação em Cadeia da Polimerase/economia , Reação em Cadeia da Polimerase/tendências , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Ribotipagem/economia , Ribotipagem/tendências , Sensibilidade e Especificidade , Especificidade da Espécie
6.
Int J Infect Dis ; 17(8): e629-33, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23402798

RESUMO

BACKGROUND: Hemophagocytosis during Q fever (QF) and Mediterranean spotted fever (MSF) is rare and only a few cases have been reported. We aimed to investigate the characteristics, outcome, and treatment of QF/MSF-associated hemophagocytosis. METHODS: We retrospectively reviewed all patients with a diagnosis of QF or MSF and suspected hemophagocytic syndrome (HS), according to Henter's criteria, between 2002 and 2011, and compared the latter to patients without HS or with lymphoma-associated HS. RESULTS: Seventeen patients with HS (median age 42 years, range 5-68 years; five females (29%)) with QF (n=8) and MSF (n=9) were included in this study. When comparing patients with QF- and MSF-associated HS with patients without HS (n=11), HS-associated signs (splenomegaly, ferritinemia, hypertriglyceridemia, and cytopenia) were significantly more frequent in patients with histological HS (p<0.05), along with a greater number of Henter's criteria. Despite the presence of HS-associated signs, treatment was similar in these two subgroups, including the time to recovery and the outcome. When compared to lymphoma-associated HS (n=10), the outcome in QF/MSF-associated HS was significantly different, with mortality in 70% of lymphoma patients versus none in QF- and MSF-associated HS (p<0.05). CONCLUSION: Hemophagocytosis is a rare occurrence during the course of QF and MSF. The presence of profound cytopenia is quite unusual in QF and MSF and should bring to mind the presence of associated HS. Nevertheless, hemophagocytic syndrome is associated with a good outcome in this condition.


Assuntos
Febre Botonosa/complicações , Linfo-Histiocitose Hemofagocítica/complicações , Febre Q/complicações , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Febre Botonosa/diagnóstico , Criança , Pré-Escolar , Feminino , Humanos , Fígado/patologia , Linfo-Histiocitose Hemofagocítica/diagnóstico , Linfo-Histiocitose Hemofagocítica/terapia , Linfoma/complicações , Masculino , Pessoa de Meia-Idade , Febre Q/diagnóstico , Estudos Retrospectivos , Adulto Jovem
7.
Eur J Clin Microbiol Infect Dis ; 31(8): 1951-60, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22231499

RESUMO

To reduce the delay in diagnosis of Q fever, we have adapted the ultrasensitive immuno-PCR method for the detection of Phase II IgM anti-Coxiella burnetii. We compared its performance to ELISA, IFA and PCR using 31 acute Q fever sera and 50 control sera. The best sensitivity was obtained by iPCR (27 out of 31) followed by PCR (18 out of 31), ELISA (12 out of 31) and IFA (10 out of 31). A specificity of 92% was found by iPCR (3 false positive out of 40), 92% for ELISA (3 false positive out of 40) whereas PCR and IFA exhibited a specificity of 100%. Among the 31 Q fever sera, we compared the four methods for the detection of the early sera sampled during the two first weeks after the onset of symptoms and found a sensitivity of 90% by iPCR, 55% for PCR, 35% for ELISA and 25% for IFA. The results presented in this study suggest that iPCR is a promising, sensitive and specific method that can be used for the early diagnosis of acute Q fever and more generally for acute infections where traditional methods lack sensitivity.


Assuntos
Técnicas de Laboratório Clínico/métodos , Reação em Cadeia da Polimerase/métodos , Febre Q/diagnóstico , Anticorpos Antibacterianos/sangue , Humanos , Imunoensaio/métodos , Imunoglobulina M/sangue , Sensibilidade e Especificidade , Testes Sorológicos/métodos
8.
Med Mal Infect ; 39(2): 71-81, 2009 Feb.
Artigo em Francês | MEDLINE | ID: mdl-19118963

RESUMO

Rickettsiae are strictly intracellular bacteria belonging to the Rickettsiaceae family. They are transmitted by various arthropods species, which are either vectors or reservoirs for the bacteria. The specific association between Rickettsieae and the vector are extensively studied, moreover Rickettsieae associated diseases are part of a continuously evolving field. Nevertheless, some rickettsiosis, such as epidemic typhus, have been described since the 16th century. Emerging diseases related to these bacteria are being investigated as well as new species, the implication of which is not always clear in human pathology when they are discovered. Some of these diseases are benign, others may be potentially fatal. Clinicians must therefore be aware of them. These issues are reviewed.


Assuntos
Infecções por Rickettsiaceae/classificação , Animais , Febre Botonosa/epidemiologia , Gatos , Infecções por Helicobacter/classificação , Infecções por Helicobacter/terapia , Humanos , Ratos , Rickettsia , Infecções por Rickettsia/classificação , Infecções por Rickettsia/terapia , Rickettsiaceae , Infecções por Rickettsiaceae/terapia
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