RESUMO
Metacercariae of Paragonimus mexicanus were collected in crabs Tehuana guerreroensis (Rathbun, 1933) in the municipality of Putla, Oaxaca, Mexico. Metacercariae were found in 20.8% of the crabs collected, with an average of 1.9 metacercarie per crab. Stained metacercariae showed the specific characteristics of P. mexicanus by morphology and sequencing a fragment of the 28S ribosomal gene obtained by PCR. These findings reveal that T. guerreroensis is an intermediate host for P. mexicanus; this new report is relevant considering the potential risk of transmission in the states of Oaxaca and Guerrero, Mexico.
Assuntos
Braquiúros/parasitologia , Metacercárias/isolamento & purificação , Paragonimus/isolamento & purificação , Animais , Sequência de Bases , Análise por Conglomerados , DNA de Helmintos/química , DNA de Helmintos/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Metacercárias/classificação , Metacercárias/genética , México , Microscopia , Dados de Sequência Molecular , Paragonimus/anatomia & histologia , Paragonimus/classificação , Paragonimus/genética , Filogenia , RNA Ribossômico 28S/genética , Análise de Sequência de DNARESUMO
This study was carried out to determine the prevalence of Demodex mites in eyelashes of people from different places from Oaxaca. One thousand and ten patients underwent epilation of eight eyelashes from each eye and they were processed to search Demodex which was found in 208 (20%) of the patients and among them male was more affected than female, 119 (57%) and 89 (43%), respectively. There was not found a relationship between clinical manifestations among these patients since 101 (49%) and 107 (51%) of them were symptomatic and asymptomatic, respectively. The range of age that was more affected was 96-105 (100%), followed by 86-95 (75%) and 76-85 (64%) years old. The less affected were individuals of 15-25 years old (11%). Demodex folliculorum was relatively high in the eyelashes of individuals in young and old people in the rural region studied. These people are generally the most vulnerable patients because they live in overcrowded conditions and poor hygiene which facilitates the direct infection. Demodex brevis was found in only one sample.