Experiments and CFD simulation of an air-conditioned tractor cabin for thermal comfort of tractor operators in Pakistan.

Tractors are manufactured without air-conditioned cabins in Pakistan. This leads to thermal discomfort for tractor operators working under direct solar exposure. Therefore, this study aimed to design and install an air-conditioned cabin on a tractor. Experiments were undertaken to evaluate the installed cabin performance under two scenarios i.e., conventional (S-I) and enhanced (S-II) air distribution. Computational fluid dynamics (CFD) simulations were used to analyze airflow and calculate thermal comfort indices. The results showed that the air-conditioned cabin attained optimum thermal conditions under the enhanced air distribution scenario (S-II). In this scenario, the inside cabin temperature was an average of 27.4 °C, compared with 30.4 °C in S-I. The relative humidity remained similar in both scenarios, around 53 %. The temperature difference between the cabin and the ambient environment was 11.09 °C in S-II, aligning with the thermal comfort conditions outlined in ISO 14269-2. Furthermore, the CFD simulations showed a predicted mean vote (PMV) index of 0.61 and the percentage people dissatisfied (PPD) index of 26.5 %. These results also confirm the provision of optimum thermal conditions for operator inside the cabin. The simulations also demonstrated good agreement with experimental data, with a small difference in air temperature (2 °C) and relative humidity (5.8 %). In the light of these findings, this study recommends installation of air-conditioned cabin on tractors with enhanced air distribution (S-II) in Pakistan to improve thermal comfort of operators.

New role of lupeol in reticence of angiogenesis, the cellular parameter of neoplastic progression in tumorigenesis models through altered gene expression.

There is a major unmet medical need for effective and well tolerated treatment options for cancer. The search now seeks to identify active biomolecules with multiple targets. Lupeol, an important dietary triterpenoid known as anticarcinogen by inducing apoptosis. But it is still more to reveal the potency of lupeol in the inhibition of neovascularization in cancer context. The study aimed to explore the efficacy of the lupeol in targeting angiogenesis. In this study, the inhibition of neovessel formation was assessed by preliminary antiangiogenesis assays like chorio allontoic membrane (CAM) and rat corneal micro pocket models. Further, validated for the micro vessel density (MVD) in histological sections of peritoneum, solid tumor and xenograft tumor by immunostaining with anti CD31 antibody. Antitumor potency was verified in ascites carcinoma, solid lymphoma and human nueroblastoma xenograft in CAM. Altered angiogenic gene expression by RT-PCR, ELISA and gelatin zymography. Lupeol significantly inhibits the neovessel formation in CAM and in the rat cornea. The similar effect was ascertained in mice and human xenograft tumor models with the regressed growth. Eventually reflecting on the differential transcription of angiogenic genes like MMP-2 & 9, HIF-1α, VEGFa and Flt-1 was noteworthy. It is now evident from our studies that, a new avenue of dietary triterpenoid lupeol by targeting angiogenesis, potentially inferring the multimode action in cancer prevention.