Ocorrência de não conformidades físico-químicas e microbiológicas em leite e derivados no estado de Minas Gerais, no período de 2011 a 2015 / Occurrence of physico-chemical and microbiological nonconformities in milk and dairy products in the State of Minas Gerais, from 2011 to 2015

Milk and dairy products are potential sources for spoilage and pathogenic microorganisms, and although a huge amount of data is collected by the official inspection services, only a few reports are available to the public. This study aimed to evaluate the data for physicochemical and microbiological quality of pasteurized milk and dairy products, collected for inspection at industrial establishments registered at the Instituto Mineiro de Agropecuária (IMA), which is an official inspection service in Minas Gerasi State, Brazil. A total of 192 analyzes were done in 2011, 1008 in 2012, 1368 in 2013, 1271 in 2014, 1582 in 2015, adding up to 5421 samples analyzed by standard analytical techniques in official government laboratories. The statistical analysis was descriptive. A total of 2010 analytical results were nonconform to the legal requirements. Among the results, 78 (4.3%) samples of mozzarella cheese were positive for alkaline phosphatase, and freezing point results for pasteurized milk were outside the legal requirements in 86 (10%) samples. Staphylococcus coagulase positive was above limits in 80 (4.4%) samples of mozzarella cheese. These results indicate a risk to the consumer's health even in pasteurized products, and the need for effective enforcement of good manufacturing practices in the food industries.(AU)

Adulterants interference on Fourier Transform Infrared analysis of raw milk / Interferência de adulterantes na análise do leite cru por espectroscopia pela transformada de Fourier

O objetivo deste trabalho foi analisar as leituras de composição do leite cru por meio de espectrofotometria FTIR, utilizando-se curva de regressão PLS, bem como as contagens de células somáticas e bacteriana total por citometria de fluxo, após adição de amido e sacarose. O leite cru foi adulterado com três concentrações de amido e sacarose (0,1%, 0,5% e 1%), colocado em frascos contendo bronopol ou azidiol, os quais foram armazenados em duas temperaturas (7±2°C e 25±2°C ). As análises foram realizadas após zero, três, 24, 48, 72 e 168 horas de armazenamento. O modelo de regressão linear múltipla foi utilizado para análise estatística. A adição de amido e sacarose resultou em mudança significativa (P<0,05) para todas as variáveis dependentes. O leite adulterado com amido resultou em aumento nas leituras de gordura, proteína, lactose, sólidos totais (ST), sólidos não gordurosos (SNG), CCS e CBT e em diminuição das leituras de caseína, do nitrogênio ureico do leite (NUL) e do ponto de congelamento. O leite adulterado com sacarose resultou no aumento das leituras da lactose, ST, SNG e NUL, enquanto as leituras de proteína, caseína, ponto de congelamento e CCS diminuíram. Este trabalho evidencia a importância do monitoramento de adulterantes reconstituintes no leite por afetarem os resultados analíticos da qualidade do leite, obtidos por métodos eletrônicos.(AU)

Oocyte viability and cortical activation under different salt solutions in Prochilodus lineatus (Teleostei: Prochilodontidae).

This study aimed to evaluate the effect of five salt solutions in the maintenance of morphological features of cortical alveolus, hydration and fertilization capacity of Prochilodus lineatus oocytes. For this purpose, five saline solutions were tested: Ringer's solution, Ringer's lactate solution, Hank's balanced salt solution (HBSS), Hank's balanced salt solution without calcium (HBSS without calcium) and solution for salmonid eggs. Oocytes were maintained for 2 hr in saline solution with controlled temperature subsequently evaluated for hydration, cortical activation and fertilization ability. In the evaluation of the fertilization ability, two controls were used: C1-fertilized oocytes after extrusion-and C2-oocytes kept in ovarian fluid and fertilized after 2 hr. There was a significant reduction in the viability of oocytes C2 (28.8% ± 12.9%) compared to C1 (65.3% ± 26.7%), and no significant differences were found between treatments HBSS and HBSS without calcium and C2. Only HBSS and HBSS without calcium maintained the non-activated state of the gametes, with a fertilization rate of 16.4% ± 6.7% and 5.6% ± 2.3%, respectively; however, they did not extend the viability of oocytes, such that they continued to undergo degradation during the storage period, similar to oocytes retained only in ovarian fluid.

Body adiposity and bone parameters of male rats from mothers fed diet containing flaxseed flour during lactation.

Obesity and osteoporosis may have their origins in early postnatal life. This study was designed to evaluate whether flaxseed flour use during lactation period bears effect on body adiposity and skeletal structure of male rat pups at weaning. At birth, male Wistar rats were randomly assigned to control and experimental (FF) groups, whose dams were treated with control or flaxseed flour diet, respectively, during lactation. At 21 days of age, pups were weaned to assess body mass, length and composition by dual-energy X-ray absorptiometry. The animals were then sacrificed to carry out analysis of serum profile, intra-abdominal adipocyte morphology and femur characteristics. Differences were considered significant when P<0.05. The FF group displayed the following characteristics (P<0.05): higher body mass, length, bone mineral content, bone area and concentrations of osteoprotegerin, osteocalcin and high-density lipoprotein cholesterol; higher levels of stearic, α-linolenic, eicosapentaenoic and docosapentaenoic acids and lower levels of arachidonic acid and cholesterol; smaller adipocyte area; and higher mass, epiphysis distance, diaphysis width, maximal load, break load, resilience and stiffness of femur. Flaxseed flour intake during lactation period promoted adipocyte hypertrophy down-regulation and contributed to pup bone quality at weaning.

Mineral cycling and pH gradient related with biological activity under transient anoxic-oxic conditions: effect on P mobility in volcanic lake sediments.

Phosphorus (P) mobility from the sediments to the water column is a complex phenomenon that is generally assumed to be mainly redox sensitive and promoted by anoxic conditions. Thus, artificial aeration of the hypolimnium has been used as a remediation technique in eutrophic water bodies but several times with unexpected disappointing results. To optimize lake restoration strategies, the aim of the present study is to assess the P flux from the sediments under transient anoxic-conditions and to identify the relevant drivers. P sequential extraction, microprofiling (of pH, O2 and H2S), and bacterial community identification were performed on a sediment microcosm approach. The results demonstrated that the overall P release from sediments to the water column during transient phase was higher during the oxic phase, mainly from pH sensitive matrixes. The microprofiles signature suggests that the observed pH gradient during the oxic phase can be a result of H2S oxidation in suboxic layers spatially separated and pared to O2 reduction in top layers, through an electroactive bacterial network. These findings point to an additional driver to be considered when assessing P mobility under transient anoxic-oxic conditions, which would derive from pH gradients, built on the microbial electrical activity in sediments from freshwaters volcanic lakes.

Intrauterine hormone effects on tooth dimensions.

The human dentition is a complex adaptive system that is influenced by genetic, epigenetic, and environmental factors. Within this system, is sexual dimorphism related to the growth promotion of the Y chromosome, or to hormonal influences, or both? This study is the first to investigate both primary and permanent tooth sizes in females from opposite-sex dizygotic (DZOS) twin pairs compared with females from dizygotic same-sex (DZSS) and monozygotic (MZ) twin pairs to indicate the influence of intrauterine male hormone, including the initial testosterone surge, on dental development. Serial dental models of the primary, mixed, and permanent dentitions of 134 females from DZOS, DZSS, and MZ twins were examined. Mesiodistal, buccolingual, crown height, and intercuspal dimensions of all primary teeth and selected permanent teeth were determined by image analysis. Univariate and multivariate analyses showed statistically significantly larger crown size in DZOS females in both dentitions, with the crown height dimensions displaying the greatest increase in size. These findings strongly support the Twin Testosterone Transfer (TTT) hypothesis. We propose that the growth-promoting effects of the Y chromosome and intrauterine male hormone levels influence different tooth dimensions and contribute differentially to the sexual dimorphism of human teeth.

Odontogenic tumours in children and adolescents: a collaborative study of 431 cases.

This study describes the oral and maxillofacial pathological characteristics of a series of odontogenic tumours in children and adolescents from three Brazilian reference centres. The records were reviewed for all odontogenic tumours in patients up to 18 years old based on criteria proposed by the World Health Organization (WHO) in 2005. Data concerning sex, age, skin colour and tumour location were collected and plotted. Four hundred and thirty one odontogenic tumours in children and adolescents were found, accounting for 37.5% of the total number of odontogenic tumours diagnosed. Benign tumours were predominant (99.8% of the cases), and odontoma was the most frequent type (41.4%), followed by keratocystic odontogenic tumours (25.5%) and ameloblastoma (14.6%). Odontogenic tumours were rarely detected in early childhood, and their prevalence increased with age. An almost equal distribution was observed with respect to sex and the site of the lesions. This study is the largest reported retrospective analysis describing odontogenic tumours in children and adolescents to date. The authors detected some variation in the relative frequency of odontogenic tumours compared with similar reports. Additional studies should be conducted based on the new WHO classification and predetermined age parameters to enable comparative analysis among different worldwide populations.

The reliability and accuracy of an electromagnetic motion analysis system when used conjointly with an accelerometer.

The effect of an accelerometer driven electronic postural monitor (Spineangel®) placed within the electromagnetic measurement field of the Polhemus Fastrak™ is unknown. This study assessed the reliability and accuracy of Fastrak™ linear and angular measurements, when the Spineangel® was placed close to the sensor(s) and transmitter. Bland Altman plots and intraclass correlation coefficient (2,1) were used to determine protocol reproducibility and measurement consistency. Excellent reliability was found for linear and angular measurements (0.96, 95% CI: 0.90-0.99; and 1.00, 95% CI: 1.00-1.00, respectively) with the inclusion of Spineangel®; similar results were found, without the inclusion of Spineangel®, for linear and angular measurements, (0.96, 95% CI: 0.89-0.99; and 1.00, 95% CI: 1.00-1.00, respectively). The greatest linear discrepancies between the two test conditions were found to be less than 3.5 mm, while the greatest angular discrepancies were below 3.5°. As the effect on accuracy was minimal, these findings support the conjoint use of the Fastrak™ during validation studies of the Spineangel® device. STATEMENT OF RELEVANCE: Although previous studies have used the Fastrak™ as the gold standard measurement system, the influence of an accelerometer driven postural monitor on accuracy has not been reported. The strength of the present study has been to determine the effect of accelerometer placement within the electromagnetic field on the reliability and accuracy of the Fastrak™.

Phosphorus fractionation in volcanic lake sediments (Azores - Portugal).

The phosphorus distribution in volcanic sediments of three lakes that are under different anthropogenic pressures in São Miguel island (Azores - Portugal) was evaluated using a sequential extraction scheme. The P-fractionation scheme employs sequential extractions of sediment with NH4Cl, bicarbonate-dithionite (BD), NaOH (at room temperature), HCl and NaOH (at 85 degrees C) to obtain five P-fractions. The P-fractionation shows that in lakes with higher trophic status (Lake Furnas and Lake Sete Cidades), the NaOH extracted P is the dominant fraction, that contribute with more than 50% to total sedimentary phosphorus. The rank order of P-fractionation for these two lakes was NaOH>NaOH (85 degrees C)>HCl>BD>NH4Cl for Furnas lake and NaOH>HCl>NaOH (85 degrees C)>NH4Cl>BD for Sete Cidades lake. On the other hand, the trend of P contribution in the oligotrophic lake Fogo shows that the most inert P pools have the higher concentrations, with more than 50% of the P contribution from the last extraction step with NaOH at 85 degrees C. For this lake, the rank order of P-fractionation was NH4Cl>BD>NaOH>HCl>NaOH (85 degrees C). The Phosphorus Maximum Solubilization Potential (P-MSP) was also calculated and the results show that for the more bio-available P-fractions (first and second extraction step), the P-MSP values for Furnas and Sete Cidades lakes are sensibly higher than the results obtained in Fogo lake, an indication of the non-point diffuse load discharged in the first ones.

Critérios mecânicos para progressão de exercícios de rotação interna e externa do ombro no plano sagital / Mechanical criteria for progression in internal and external rotation exercises of the shoulder in the sagittal plane

INTRODUÇÃO: O conhecimento da capacidade de produção de torque e de força, do comportamento da distância perpendicular ao longo do movimento e a influência destas sobre o comportamento do torque produzido é essencial para o entendimento do movimento humano e pode ser de grande utilidade para controlar a sobrecarga imposta à estrutura musculotendínea. OBJETIVO: Apresentar critérios mecânicos para a progressão de exercícios de rotação interna (RI) e externa (RE) do ombro no plano sagital. MÉTODO: Seis indivíduos foram avaliados através de um dinamômetro isocinético e de um eletrogoniômetro. A partir dos dados obtidos na coleta, foram calculadas as médias de torque, força resultante e a distância perpendicular média ponderada (DPMP) nos softwares SAD32 e Matlab®. RESULTADOS: Os ângulos em que ocorreram os picos de torque de RE e RI foram de -34º e 6º, com valores de 43 Nm e 69 Nm respectivamente, e os picos de força muscular de RE e RI ocorreram nos 35º e -14º, sendo que os valores nessas angulações foram de 10227 N e 8464 N, respectivamente. A DPMP dos RE apresentou um comportamento crescente ao longo de toda amplitude de movimento (ADM), tendo seu pico no final da amplitude, ou seja, em -50º (0,91cm); e a DPMP dos RI apresentou-se praticamente constante, com seu maior valor aos 50º (0,96cm). CONCLUSÕES: Os critérios mecânicos para a progressão de exercícios de rotação interna e externa são: torque, força e distância perpendicular média ponderada, porque, baseado em seus comportamentos, é possível promover diferentes sobrecargas sobre a estrutura musculotendínea.

INTRODUCTION: Knowledge of torque and force production capacity and moment arm patterns throughout the movement, and their influence on the torque produced, are essential for understanding human movement and may be of great use for controlling the overload imposed on the muscle-tendon structure. OBJECTIVE: To present mechanical criteria for progression in internal rotation (IR) and external rotation (ER) exercises of the shoulder in the sagittal plane. METHOD: Six individuals were assessed using an isokinetic dynamometer and an electrogoniometer. From the data collected, the mean torque, mean resultant force and weighted mean moment arm were calculated using the SAD32 and Matlab® software. RESULTS: The angles at which the peak ER and IR torque occurred were -34º and 6º with values of 43 Nm and 69 Nm, respectively. The peaks for ER and IR muscle force were at 35º and -14º, and the values at these angles were 10227 N and 8464 N, respectively. The weighted mean moment arm for ER presented an increasing pattern over the whole range of motion (ROM) and the peak was at the end of the ROM, i.e. at -50º (0.91 cm). The weighted mean moment arm for IR was almost constant with its peak at 50º (0.96 cm). CONCLUSION: The mechanical criteria for progression in internal and external rotation exercises of the shoulder are torque, force and weighted mean moment arm because different overloads on the muscle-tendon structure can be caused according to their patterns over the ROM.

Acute haemorrhagic conjunctivitis outbreak in the city of Fortaleza, northeast Brazil.

BACKGROUND/AIM: Between February and May 2003 an epidemic of acute haemorrhagic conjunctivitis affected more than 200 000 people in all five geographic regions of Brazil (north, south, midwestern, southeast, and northeast). The aim was to identify the aetiological agent and to describe clinical aspects of this outbreak in a group of patients treated at the ophthalmology department of the Hospital Walter Cantídio (OD-HWC) at the Universidade Federal do Ceará, in the city of Fortaleza, capital of the state of Ceará, northeastern Brazil. METHODS: Conjunctival swabs were collected from patients who spontaneously went to the laboratory of virology. Specimens were inoculated in HEp-2 and RD cell lines. The viral isolation was confirmed by performing reverse transcriptase polymerase chain reaction and indirect immunofluorescence assay. RESULTS: Viral conjunctivitis was diagnosed in 56 patients but only 24 of them allowed the collection of samples. Of 24 conjunctival swabs tested, 11 were positive for a variant of coxsackie virus A24 (CA24v) and one of the isolates reacted with anti-adenovirus monoclonal antibodies. CONCLUSION: CA24v was confirmed as the aetiological agent of this outbreak of acute haemorrhagic conjunctivitis in the city of Fortaleza.

Postprandial triacylglycerol responses in simulated night and day shift: gender differences.

A number of reports suggest that shift workers have an increased risk of coronary heart disease (CHD). One contributing factor may be the consumption of meals at night with consequent altered postprandial responses. This study investigated circulating triacylglycerol (TAG), a possible risk factor for CHD, after meals during a simulated day and night shift. Twenty-five healthy participants (10 women and 15 men) were studied. They were given a pre-meal at 0800 h and a test meal at 1330 h on a simulated day shift and then an identical pre-meal at 2000 h and test meal at 0130 h, respectively, on a simulated night shift with maintained wakefulness. Blood was sampled for 9 h after the test meal for analysis of basal and postprandial plasma TAG levels. ANOVA for repeated measures indicated higher TAG in men compared with women (p < 0.0001) and higher responses at night in both genders (p = 0.027). Incremental area under the curve (IAUC) analysis indicated that men had significantly increased postprandial TAG levels at night compared with the day: (IAUC 0-540 min, mean +/- SEM) 253.29 +/- 28.73 versus 148.33 +/- 17.28 mmol/L x min, respectively, p = 0.025. In women, night and day responses (61.16 +/- 8.93 versus 34.09 +/- 7.87 mmol/L x min, respectively, p = 0.457) were not significantly different. Circulating TAG remained elevated for longer at night in the men compared with the women (p = 0.009). This study demonstrates the existence of gender and time-of-day differences in TAG responses to a meal. These raised TAG levels at night, for a prolonged time in men, may be relevant to the increased risk of CHD in shift workers.

Altered postprandial hormone and metabolic responses in a simulated shift work environment.

The circadian rhythms of most night shift workers do not adapt fully to the imposed behavioural schedule, and this factor is considered to be responsible for many of the reported health problems. One way in which such disturbances might be mediated is through inappropriate hormonal and metabolic responses to meals, on the night shift. Twelve healthy subjects (four males and eight females) were studied on three occasions at the same clock time (1330 h), but at different body clock times, after consuming test meals, first in their normal environment, secondly after a forced 9 h phase advance (body clock time approximately 2230 h) and then again 2 days later in the normal environment. They were given a low-fat pre-meal at 0800 h, then a test meal at 1330 h with blood sampling for the following 9 h. Parameters measured included plasma glucose, non-esterified fatty acids (NEFAs), triacylglycerol (TAG), insulin, C-peptide, proinsulin and glucose-dependent insulinotropic polypeptide, and urinary 6-sulphatoxymelatonin. In contrast with a previous study with a high-fat pre-meal, postprandial glucose and insulin responses were not affected by the phase shift. However, basal plasma NEFAs were lower immediately after the phase shift (P < 0.05). Incremental (difference from basal) TAG responses were significantly higher (P < 0.05) immediately after the phase shift compared with before. Two-day post-phase shift responses showed partial reversion to baseline values. This study suggests that it takes at least 2 days to adapt to eating meals on a simulated night shift, and that the nutritional content of the pre-meals consumed can have a marked effect on postprandial responses during a simulated phase shift. Such findings may provide a partial explanation for the increased occurrence of cardiovascular disease reported in shift workers.