Dual-color deep-tissue three-photon microscopy with a multiband infrared laser.

Multiphoton microscopy combined with genetically encoded fluorescent indicators is a central tool in biology. Three-photon (3P) microscopy with excitation in the short-wavelength infrared (SWIR) water transparency bands at 1.3 and 1.7 µm opens up new opportunities for deep-tissue imaging. However, novel strategies are needed to enable in-depth multicolor fluorescence imaging and fully develop such an imaging approach. Here, we report on a novel multiband SWIR source that simultaneously emits ultrashort pulses at 1.3 and 1.7 µm that has characteristics optimized for 3P microscopy: sub-70 fs duration, 1.25 MHz repetition rate, and µJ-range pulse energy. In turn, we achieve simultaneous 3P excitation of green fluorescent protein (GFP) and red fluorescent proteins (mRFP, mCherry, tdTomato) along with third-harmonic generation. We demonstrate in-depth dual-color 3P imaging in a fixed mouse brain, chick embryo spinal cord, and live adult zebrafish brain, with an improved signal-to-background ratio compared to multicolor two-photon imaging. This development opens the way towards multiparametric imaging deep within scattering tissues.

Supercontinuum-seeded few-cycle mid-infrared OPCPA system.

We propose and demonstrate an OPCPA architecture emitting few-cycle pulses at 3070 nm and 1550 nm based on a high-energy femtosecond ytterbium-doped fiber amplifier pump. The short pump pulse duration allows direct seeding by a supercontinuum in the 1.4 - 1.7 µm signal range, generated in bulk YAG. It also allows a simplified dispersion management along the system and broad optical gain bandwidth. The dual output system delivers 20 µJ, 49 fs signal pulses at 1550 nm and 10 µJ, 72 fs idler pulses at 3070 nm. Power scaling limitations due to beam distortion in the last MgO:PPLN-based OPCPA stage are discussed and investigated.

Coherent beam combining with an ultrafast multicore Yb-doped fiber amplifier.

Active coherent beam combination using a 7-non-coupled core, polarization maintaining, air-clad, Yb-doped fiber is demonstrated as a monolithic and compact power-scaling concept for ultrafast fiber lasers. A microlens array matched to the multicore fiber and an active phase controller composed of a spatial light modulator applying a stochastic parallel gradient descent algorithm are utilized to perform coherent combining in the tiled aperture geometry. The mitigation of nonlinear effects at a pulse energy of 8.9 µJ and duration of 860 fs is experimentally verified at a repetition rate of 100 kHz. The experimental combining efficiency results in a far field central lobe carrying 49% of the total power, compared to an ideal value of 76%. This efficiency is primarily limited by group delay differences between cores which is identified as the main drawback of the system. Minimizing these group delay issues, e.g. by using short and straight rod-type multicore fibers, should allow a practical power scaling solution for femtosecond fiber systems.