Plastic webs, the new food: Dynamics of microplastics in a Mediterranean food web, key species as pollution sources and receptors.

In the context of global environmental change, this study presents a novel approach to evaluating microplastic (MP) fluxes and probabilities of pollution within marine food webs. A topological model was built to understand the dynamics of MP pollution in the Mediterranean food webs. The analysis involves two approaches: the first approach includes centrality measures to understand the key role of species in the transmission of trophic effects regarding MPs, and the second approach incorporates MP data by developing the Interaction Pollution Indices (IPIs) at multiple levels to identify species being sources and receptors of MP pollution in the new concept of a plastic-food web. The trophic network consisted of 356 nodes representing not only species, but also aggregations in higher taxa, for a total of 3517 interactions, with 108 species having information on MP frequency of occurrence (FO). The mean probability of dietary MP transference was 0.087 %, and the maximum was 18 %. Species such as the rose shrimp A. antennatus, the catshark S. canicula, the sole S. solea, the sardine S. pilchardus, the Norway lobster N. norvegicus, and the forkbeard P. phycis were found to be significant sources of pollution and played crucial roles in the transmission of effects within the network. By incorporating the IPIs, a deeper understanding of the pollution dynamics at multiple levels was gained, highlighting the value of combining feeding and MP pollution data to develop effective management and conservation strategies. The application of the IPIs holds immense potential for studying bioaccumulation and biomagnification through MP pollutant transferences in marine ecosystems. Its flexibility in incorporating different types of information and units enables its transversal application throughout the field of ecology. This research provides a crucial step towards developing effective tools for MP pollution mitigation strategies and the preservation of marine ecosystems integrity.

Monitoring microplastics in coastal waters of a biosphere reserve: a case study in Menorca (Spain).

This study provides with evidence of the presence of sea surface microplastics in a UNESCO marine biosphere reserve: the island of Menorca in the north-western Mediterranean Sea. From a total of 90 samples, in 100% of the samples, microplastics were observed with a mean value of 0.18 ± 0.01 items/m2. According to data, no significant differences were observed for sampling period with very similar values between 2021 (0.17 ± 0.02 items/m2) and 2022 (0.18 ± 0.02 items/m2). However, significant differences were observed regarding sampling area (both site and locality) suggesting that sea surface plastics in the study area might be more dependent of the spatial scale rather than on the temporal scale. Fibre type microplastics predominated over fragments, films, pellets, and foams, but in the commercial Port de Maó, almost 50% of the identified items were foams which could be related to the transportation of packed goods to this port. Results from the model applied to study the relation between waste management indicators and microplastic abundance indicate that when considering all marine litter categories, the explanatory variables are plastic waste generated by residents population (tonnes/year/km2) and waste collection rate (%), whereas if only plastics are considered, the indicator regarding waste per capita (kg/hab/year) is also included. Data in this study is obtained through a harmonized protocol which can be used to define baseline and threshold values to evaluate good environmental status regarding descriptor 10 of the Marine Strategy Framework Directive.

Stranded and floating marine debris detected along the coastline of Cabrera National Park (Balearic Islands).

Marine Protected Areas (MPAs) are managed to conserve ecosystems however increased reporting highlights the observations of marine debris within these areas. The coastline of Cabrera Natural Park (Cabrera MPA) was surveyed in February, March, May, and July of 2021 to evaluate the seasonal trend of marine debris between winter and summer months. A general value of 6.94 items/km, ranging from 4.38 ± 4.55 items/km in July to 12.57 ± 17.56 items/km in March, was detected with no statistical differences between areas or surveyed months. Abundance of stranded debris (77 %) was statistically higher than floating debris (23 %). Prevailed floating plastic pieces 2.5 >< 50 cm and stranded nets and pieces of nets >50 cm. Artificial polymer materials, non-sourced debris, and non-Single Use Plastics were the most common items identified. This study highlights the importance of applying mitigation measures to avoid the presence of marine debris in areas of ecological interest.

Sea surface surveys for microplastic and floating marine macro litter items in coastal waters of Cabrera Archipelago Maritime Terrestrial National Park.

This study is aimed at identifying and comparing microplastics and floating marine litter along the sea surface in the marine protected area of Cabrera Archipelago Maritime Terrestrial National Park (Cabrera MPA) in the Balearic Islands. A total of 52 net surveys and 22 visual surveys were carried out between July and August in 2019 and 2020. The abundance of microplastic (MP) items was highest in the southern and eastern regions, with an average of 381,244.4 ± 1,031,082.8 items/km2 weighing an average of 927.1 ± 2731.4 g/km2. Most of these items were < 5 mm (81%) in size and were mainly composed of polyethylene and polypropylene (98%). In terms of floating marine macro litter (ML) from visual surveys, an average of 2028 ± 2084 items/km2 were observed. In this case, the majority of the ML items were plastic pieces (69%) measuring 2.5 to 50 cm. Furthermore, ML quantified by visual surveys was an order of magnitude higher than in similar studies carried out on large vessels, highlighting the importance of vessel height and speed for identifying the smallest size fractions (81%). The results of this study document the intensity of MPs and ML, primarily plastic, in coastal waters, and provide a baseline for management efforts to mitigate floating litter, in addition to raising awareness of the transferability of marine litter from other regions.

Are seafloor habitats influencing the distribution of microplastics in coastal sediments of a Marine Protected Area?

The marine environment is affected by the increasing presence of microplastics (MPs; < 5 mm), and the seafloor acts as a sink for these particles. Locations with different predominant seafloor habitat and protection level applied were selected from Cabrera Marine-Terrestrial National Park (henceforth, Cabrera MPA) (western Mediterranean Sea) with the aim to assess the distribution of MPs along the sediments of this Mediterranean MPA. A total of 37 samples were collected. A high diversity of sediment between locations was detected according to the Udden-Wentworth classification and locations were clustered into two main groups according to the predominance of different particle size fractions. The identification of MPs was carried out according to the sediment particle size classification. A total of 1431 MPs and a mean value (± SD) of 314.53 ± 409.94 items kg-1 D.W. were identified, and 70% of the particles were fibers. Statistically higher abundances of MPs were found in sediments collected from sandy habitats, with a mean value of 630.80 ± 636.87 items kg-1 D.W., compared to the abundances of MPs found in locations with different predominant seafloor habitats, that ranged from 136.79 ± 156.33 items kg-1 D.W. in habitats with similar predominance of seagrass and sand to 223.02 ± 113.35 items kg-1 D.W. in habitats with similar predominance of rocks and sand. The abundance of MPs regarding each sediment particle size fraction differed between years and locations, and the abundance of MPs according to each identified shape differed between sampling years, particle size fraction, and predominant seafloor habitat. The present study highlights the ubiquitous presence of MPs in seafloor sediments from a MPA. Furthermore, the results suggest that the predominant seafloor habitat can modulate the presence of MPs in marine environments in both general abundances and shape of items.

Elucidating the consequences of the co-exposure of microplastics jointly to other pollutants in bivalves: A review.

The marine environment has numerous impacts related to anthropogenic activities including pollution. Abundances of microplastics (MPs) and other pollutants are continuously increasing in the marine environment, resulting in a complex mixture of contaminants affecting biota. In order to understand the consequences, a review of studies analyzing combined effects of MPs and other types of pollutants in bivalves has been conducted as species in this group have been considered as sentinel and bioindicators. Regarding studies reviewed, histological analyses give evidence that MPs can be located in the haemolymph, gills and gonads, as well as in digestive glands in the intestinal lumen, epithelium and tubules, demonstrating that the entire body of bivalves is affected by MPs. Moreover, DNA strand breaks represent the most relevant form of damage caused by the enhanced production of reactive oxygen species in response to MPs exposure. The role of MPs as vectors of pollutants and the ability of polymers to adsorb different compounds have also been considered in this review highlighting a high variability of results. In this sense, toxic impacts associated to MPs exposure were found to significantly increase with the co-presence of antibiotics or petroleum hydrocarbons amongst other pollutants. In addition, bioaccumulation processes of pollutants (PAHs, metals and others) have been affected by the co-presence with MPs. Histological, genetic and physiological alterations are the most reported damages, and the degree of harm seems to be correlated with the concentration and size of MP and with the type of pollutant.

A Biomarker Approach as Responses of Bioindicator Commercial Fish Species to Microplastic Ingestion: Assessing Tissue and Biochemical Relationships.

Plastic debris is a growing environmental problem on a global scale, as plastics and microplastics (MPs) can be ingested by marine organisms, inducing toxic effects. The aim of this study was to assess MP intake and antioxidant responses in three bioindicator species: red mullet, bogue, and anchovy (Mullus surmuletus, Boops boops, and Engraulis encrasicolus, respectively) for plastic contamination in the Mediterranean Sea. MP intake was assessed in the gastrointestinal tract of the fish. Further, several enzymes from both the liver and brain were analysed. The antioxidant defences, catalase (CAT) and superoxide dismutase (SOD), as well as the detoxifying enzyme glutathione-S-transferase (GST), were measured in both tissues. The acetylcholine esterase (AchE), as an indicator of neuronal damage, was measured in the brain. Malondialdehyde (MDA) was analysed as a marker of oxidative damage in the brain and liver samples. Total MP intake and MP typology differed between the three species, with M. surmuletus showing the lowest intake of MPs, while B. boops showed the highest intake of MPs. An increase in both antioxidant enzymes was evidenced in E. encrasicolus liver activity with respect to MP intake. In brain samples, an increase in CAT activity was found in M. surmuletus and B. boops as a consequence of MP ingestion. SOD activity in the brain increased in B. boops and E. encrasicolus that had ingested MPs. GST activity increased in the liver of M. surmuletus' and in brains of B. boops that had ingested MPs. The intake of MPs is species related, as well as being inherently linked to the habitat they live in and being able to induce a light activation of species-specific detoxifying and antioxidant mechanisms.

Multispecies Assessment of Anthropogenic Particle Ingestion in a Marine Protected Area.

We have applied a multispecies ecosystem approach to analyse the ingestion of anthropogenic particles (AP) in the gastrointestinal tract of 313 individuals (17 fish species and 8 invertebrate species) from pelagic, demersal and benthic habitats in a marine protected area off the Western Mediterranean (Cabrera National Park). We have quantified and characterized the ingestion at several taxonomic levels of fish, sea urchins, sea cucumbers, bivalves, and jellyfish in relation to biotic/abiotic factors based on taxonomic groups, trophic guilds (functional groups) and habitats. AP ingestion occurrence ranged from 26 to 100% with no significant differences among taxonomic groups. The fish within the MPA showed an overall ingestion occurrence ranging from 0 to 100%, the echinoderms from 29 to 100%, the bivalves from 72 to 96% and the jellyfish 36% ingestion. The ecosystem approach applied to evaluate overall AP ingestion within the species reported that for trophic guilds, the omnivorous species ingested the highest amounts of anthropogenic items, while herbivores ingested significantly fewer items than all other trophic guilds. Moreover, no significant differences were found amongst habitats, indicating a homogeneous spatial distribution of APs at all studied habitats. The multispecies approach provided insight into the high APs exposure to species within Cabrera MPA, highlighting the potential harm linked with marine litter that threatens marine biodiversity.

Assessing microplastic ingestion and occurrence of bisphenols and phthalates in bivalves, fish and holothurians from a Mediterranean marine protected area.

Microplastic (MP) ingestion, along with accumulated plasticizers such as bisphenol A (BPA), bisphenol F (BPF), and bisphenol S (BPS), and phthalates represented by diethyl phthalate (DEP), dibutyl phthalate (DBP) and bis (2-ethylhexyl) phthalate (DEHP), were quantified in bivalves, fish, and holothurians collected from a coastal pristine area at the western Mediterranean Sea. MP ingestion in sediment-feeders holothurians (mean value 12.67 ± 7.31 MPs/individual) was statistically higher than ingestion in bivalves and fish (mean 4.83 ± 5.35 and 3 ± 4.44 MPs/individual, respectively). The main ingested polymers were polyethylene, polypropylene, and polystyrene. The levels of BPS, BPF, and DEHP were highest in bivalves' soft tissue; BPA and DBP had the highest levels in the holothurians' muscle. In addition, the levels of all plasticizers assessed were lowest in fish muscle; only BPA levels in fish were higher than in bivalves, with intermediate values between those of bivalves and holothurians. This study provides data on exposure to MPs and plasticizers of different species inhabiting Cabrera Marine Protected Area (MPA) and highlights the differences in MP ingestion and levels of plasticizers between species with different ecological characteristics and feeding strategies.

Assessment of the impact of aquaculture facilities on transplanted mussels (Mytilus galloprovincialis): Integrating plasticizers and physiological analyses as a biomonitoring strategy.

The growing plastic production and its continuous use is a significant problem. In addition, aquaculture practices have experienced a considerable growth and plastic is widely used in these activities, hence plasticizers must be considered due to their potential ecotoxicological impacts on species. Mussels placed inside an Integrated Multi-Trophic Aquaculture (IMTA) system and at two control locations were employed to quantify the ingestion of anthropogenic particles and associated chemical plasticizers, such as bisphenol A (BPA) jointly to bisphenol F (BPF) and bisphenol S (BPS), and phthalates represented by diethyl phthalate (DEP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP). In addition, some metabolism and oxidative stress related parameters were measured in mussels' whole soft tissue. Anthropogenic particle ingestion of mussels increased over time at the three locations and the following order of abundance of pollutants was observed: BPA> BPF> DEHP> DBP> BPS> DEP. Even though no differences according to location were found for pollutants' occurrence, time trends were evidenced for BPA and DEHP. On the other hand, a location effect was observed for biomarkers with highest values detected in mussels located at the vicinities of the aquaculture facility. In addition, a reduced detoxification activity was observed over time parallel to BPA decrease.

Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) occurrence in Sparus aurata exposed to microplastic enriched diets in aquaculture facilities.

Organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs) are highly stable and bioaccumulative, and microplastics (MPs; plastics <5 mm) are ubiquitous in the marine environment. In this study we report the levels of a selection of pollutants in liver and muscle of juvenile gilthead seabream (Sparus aurata) exposed to virgin and weathered MP enriched diets during three months and followed by one month of MP depuration. In general, a major concentration of pollutants in liver was observed. According to OCPs, total DDTs was two or three times higher in liver. Levels in muscle suffer higher variability between treatments and sampling periods. The MP index was negatively correlated to HCB and positively to p,p'DDT in liver. Levels of pollutants are correlated to biological parameters such as total size and total weight. Our results suggest that the bioaccumulation is subjected to the molecular structure of the pollutants and that MPs are correlated with the detoxification system.

Microplastic ingestion in reared aquaculture fish: Biological responses to low-density polyethylene controlled diets in Sparus aurata.

During the last years, ingestion of microplastics (MPs) has been quantified in marine species both with an ecological and commercial interest at sea and under experimental conditions, highlighting the importance to assess MP ingestion in commercially and aquaculture important species such as gilthead seabream (Sparus aurata) fish. In order to study the ingestion of MPs in a commercially valuable species, gilthead seabreams were exposed to an enriched diet with virgin and weathered low-density polyethylene (LDPE) pellets for three months followed by a detoxification period of one month of no exposure to MP enriched diets. Our results indicate that MP ingestion in these fishes increased with exposure time, and differences were found between treatments, showing the highest ingestion values after three months of exposure to MP enriched diets and in the weathered treatment. However, after one month of detoxification, no MPs were found in the gastrointestinal tracts of fish, reflecting no long-term retention of MPs in Sparus aurata digestive system. According to results from this study, exposure of fish to MP enriched diets does not affect fish size neither the Fulton's condition index as both parameters increased with time in all treatments (control, virgin and weathered). Both carbon and nitrogen isotopic signatures decreased with fish size in all treatments which could be related to an increase of nitrogen deposition efficiency in fish muscle with a high protein assimilation during the first months of Sparus aurata.

Experimental evidence of physiological and behavioral effects of microplastic ingestion in Sparus aurata.

Increasing global research has identified microplastics (MPs) to be impacting marine organisms. The present work aimed at investigating the physiological and behavioral effects of thirty-six juvenile Sparus aurata exposed to control, virgin and weathered MPs enriched diets during a 21-day period under controlled conditions. Physiological effects were assessed in liver and brain using the following biomarkers: activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd), the detoxifying enzyme glutathione S-transferase (GST) and malondialdehyde (MDA) as indicative of lipid peroxidation. Individuals were recorded for behavior analysis (i.e. social interactions and feeding behavior). Results revealed an increase in cellular stress from control to weathered fish groups, with the virgin group showing intermediate levels in all quantified biomarkers. Significant differences were found in the liver for all biomarkers except for MDA, suggesting that exposure time to MPs in this experiment is long enough to trigger the activation of antioxidant enzymes but not to produce cell damage by lipid peroxidation. In brain tissue samples, fish from the weathered group presented significantly higher values for CAT and SOD, highlighting its function as primary antioxidants. Regarding behavioral effects, results showed that the two MPs enriched groups were significantly bolder during social interactions and, although no significantly, tended to be more active during feeding. In conclusion, MPs which have been weathered in marine environmental conditions produces a higher physiological response than virgin MPs but also, a physiological response is variable depending on the tissue analyzed. In addition, a short period to MP exposure seems to affect overall social and feeding behavior but, further research is needed to assess long-term effects of MP ingestion and its potential consequences on fish populations.

Anthropogenic particles ingestion in fish species from two areas of the western Mediterranean Sea.

The Mediterranean Sea is one of the most polluted seas in terms of marine debris. To analyze the ingestion of anthropogenic particles in two areas, 197 gastrointestinal tracts from four fish species - Trachurus mediterraneus, Sardina pilchardus, Engraulis encrasicolus and Boops boops - were studied. 127 anthropogenic particles were identified in the gastrointestinal tract of 28% of the samples using visual sorting methods. Individuals from the peninsular coast showed higher ingestion occurrence (36%) than those from the Balearic Islands (12%). Significant differences in the ingestion of anthropogenic particles were found between species with Trachurus mediterraneus identified as the most affected species (43% of the individuals with mean values of 1.13 ±â€¯0.16 particles/individual), and Engraulis encrasicolus, the least affected (2.56% and 0.03 ±â€¯0.16 particles/individual). Moreover, the proportion of ingestion amongst species was similar in both areas, highlighting the importance of studying the same species at different locations as marine debris bioindicators.