RESUMO
The Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) is an international public repository for high-throughput microarray and next-generation sequence functional genomic data sets submitted by the research community. The resource supports archiving of raw data, processed data and metadata which are indexed, cross-linked and searchable. All data are freely available for download in a variety of formats. GEO also provides several web-based tools and strategies to assist users to query, analyse and visualize data. This article reports current status and recent database developments, including the release of GEO2R, an R-based web application that helps users analyse GEO data.
Assuntos
Bases de Dados Genéticas , Perfilação da Expressão Gênica , Genômica , Sequenciamento de Nucleotídeos em Larga Escala , Internet , Análise de Sequência com Séries de OligonucleotídeosRESUMO
NCBI's Conserved Domain Database (CDD) is a resource for the annotation of protein sequences with the location of conserved domain footprints, and functional sites inferred from these footprints. CDD includes manually curated domain models that make use of protein 3D structure to refine domain models and provide insights into sequence/structure/function relationships. Manually curated models are organized hierarchically if they describe domain families that are clearly related by common descent. As CDD also imports domain family models from a variety of external sources, it is a partially redundant collection. To simplify protein annotation, redundant models and models describing homologous families are clustered into superfamilies. By default, domain footprints are annotated with the corresponding superfamily designation, on top of which specific annotation may indicate high-confidence assignment of family membership. Pre-computed domain annotation is available for proteins in the Entrez/Protein dataset, and a novel interface, Batch CD-Search, allows the computation and download of annotation for large sets of protein queries. CDD can be accessed via http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml.
Assuntos
Bases de Dados de Proteínas , Estrutura Terciária de Proteína , Sequência de Aminoácidos , Sequência Conservada , Modelos Biológicos , Proteínas/classificação , Análise de Sequência de ProteínaRESUMO
This project investigated the use of electronic patient documentation in the work context of VA nurses, guided by cognitive work analysis methodology. Prominent themes in nursing workflow and documentation usage identifed in previous research were summarized and presented in detail to a focus group of practicing nurses. The group validated a need for integrated access to order, prescription and document information. A wire frame solution was developed and presented in a second group meeting for evaluation and feedback.
