Biological parameters for quality evaluation of allografts from the Brazilian National Institute of Traumatology and Orthopedics tissue bank.

Bone allografts are clinically used in a variety of surgical procedures, and tissue banks are responsible for harvesting, processing, quality testing, storing, and delivering these materials for transplantation. In tissue banks, the bone is processed for the removal of all organic content, remaining only the tissue structure (scaffold). However, several studies have shown that even after using different processing methods, viable cells, functional proteins, and DNA may still persist in the tissue, which constitute the main causes of graft rejection. Therefore, the objective of this study was to establish techniques and biological parameters for quality validation of allografts. To this end, we propose the use of 3 combined methods such as microscopy, histology, and molecular biology techniques to evaluate the quality of allografts harvested and processed by the Brazilian National Institute of Traumatology and Orthopedics (INTO) tissue bank according to the donation criteria of the Brazilian National Health Surveillance Agency and the Brazilian National Transplant System. Bone fragments from different processing stages showed no viable cells on histology, an intact extracellular matrix on scanning electron microscopy, and gradual reduction in DNA amount. Different techniques were used to demonstrate the quality of allografts produced by the INTO tissue bank and to establish biological parameters for ensuring the safety and quality of these products. Future studies need to be undertaken to assess and validate the efficacy of the decellularization process in larger bone grafts with diverse architectural configurations.

A new option for bone regeneration: a rapid methodology for cellularization of allograft with human bone marrow stromal cells with in vivo bone-forming potential.

The treatment of severe musculoskeletal injuries, such as loss of bone tissue and consolidation disorders, requires bone transplantation, and the success of this bone reconstruction depends on the grafts transplant's osteogenic, osteoconductive, and osteoinductive properties. Although the gold standard is autograft, it is limited by availability, morbidity, and infection risk. Despite their low capacity for osteoinduction and osteogenesis, decellularized bone allografts have been used in the search for alternative therapeutic strategies to improve bone regeneration. Considering that bone marrow stromal cells (BMSCs) are responsible for the maintenance of bone turnover throughout life, we believe that associating BMSCs with allograft could produce a material that is biologically similar to autologous bone graft. For this reason, this study evaluated the osteogenic potential of bone allograft cellularized with BMSCs. First, BMSC was characterized and allograft decellularization was confirmed by histology, scanning electron microscopy, and DNA quantification. Subsequently, the BMSCs and allografts were associated and evaluated for adhesion, proliferation, and in vitro and in vivo osteogenic potential. We demonstrated that, after 2 hours, BMSCs had already adhered to the surface of allografts and remained viable for 14 days. In vitro osteogenic assays indicated increased osteogenic potential of allografts compared with beta-tricalcium phosphate (ß-TCP). In vivo transplantation assays in immunodeficient mice confirmed the allograft's potential to induce bone formation, with significantly better results than ß-TCP. Finally, our results indicate that allograft can provide structural support for BMSC adhesion, offering a favorable microenvironment for cell survival and differentiation and inducing new bone formation. Taken together, our data indicate that this rapid methodology for cellularization of allograft with BMSCs might be a new therapeutic alternative in regenerative medicine and bone bioengineering.

Vertical femoral neck fractures in young adults: a closed fixation strategy using a transverse cancellous lag screw.

Vertical femoral neck fractures (Pauwels type III classification) in young adults generally occur as a consequence of high-energy trauma and are frequently seen in association with multiple injuries. Considering the controversies regarding the optimal fixation for this fracture, our aim was to evaluate the clinical outcome of a closed fixation strategy for vertical femoral neck fractures in young adults using two parallel and one transverse cancellous lag screws. This was a single-surgeon, prospective study including 20 young adults with average age of 38.75 years (range 18-59 years) with a high-energy Pauwels III femoral neck fracture. Closed reduction and internal fixation with three cancellous lag screws were performed. The first screw was inserted crosswise to avoid further shear forces. Second and third parallel screws were placed above the lesser trochanter and centrally on the greater trochanter, respectively. Clinical outcomes were assessed by comparing postoperative and final follow-up radiographs 24 months post-injury. Eleven patients had an isolated vertical femoral neck fracture. Of these, five had further femoral neck comminution. Nine patients had an associated ipsilateral femoral shaft fracture. All fractures were displaced at the time of the first radiological evaluation. Closed reduction quality was considered excellent or good in 15 patients. After 24 months, bone union was achieved in 16 cases. Osteonecrosis of the femoral head developed in association with two fractures, and a nonunion developed in association with two fractures. We conclude that vertical high-energy femoral neck fractures can be treated successfully with internal fixation with two parallel cancellous lag screws positioned above the lesser trochanter and a third screw inserted centrally on the greater trochanter at an angle perpendicular to the fracture line.

Interprosthetic femoral fractures: proposed new classification system and treatment algorithm.

Interprosthetic femoral fracture is a rare and challenging fragility fracture issue. Due to aging of the population, the incidence of this type of fracture is gradually and constantly increasing. There is no complete and specific interprosthetic femoral fracture classification system that indicates treatment and prognosis in the literature. The aim of the present study was to describe a new classification system for interprosthetic femoral fractures, and to present a case series and a treatment algorithm derived from the current evidence in the literature.

Clavicle fractures - incidence of supraclavicular nerve injury / Fraturas da clavicula - incidencia de lesao do nervo supraclavicular

OBJECTIVE: To analyze retrospectively 309 fractures in the clavicle and the relation with injury of the supraclavicular nerve after trauma. METHODS: It was analyzed 309 patients with 312 clavicle fractures. The Edinburgh classification was used. Four patients had fractures in the medial aspect of the clavicle, 33 in the lateral aspect and 272 in the diaphyseal aspect and three bilateral fractures. RESULTS: 255 patients were analyzed and five had paresthesia in the anterior aspect of the thorax. Four patients had type 2 B2 fracture and one type 2 B1 fracture. All patients showed spontaneous improvement, in the mean average of 3 months after the trauma. CONCLUSION: Clavicle fractures and/ or shoulder surgeries can injure the lateral, intermediary or medial branches of the supraclavicular nerve and cause alteration of sensibility in the anterior aspect of the thorax. Knowledge of the anatomy of the nerve branches helps avoid problems in this region. .

OBJETIVO: Analisar retrospectivamente 309 fraturas da clavícula e sua relação com a lesão do nervo supraclavicular após trauma. MÉTODOS: Foram analisados 309 pacientes com 312 fraturas da clavícula. Foi usada a classificação de Edinburgh. Quatro pacientes apresentavam fraturas da região medial da clavícula, 33 da região lateral, 272 da região diafisária e três com fraturas bilaterais. RESULTADOS: Foram analisados 255 pacientes e cinco apresentavam parestesia na região anterior do tórax. Quatro pacientes apresentaram fratura do tipo 2 B2 e um do tipo 2 B1. Todos os pacientes tiveram melhoria espontânea, em média de três meses após o trauma. CONCLUSÃO: Fraturas da clavícula e/ou cirurgias no ombro podem lesar os ramos lateral, intermediário ou medial do nervo supraclavicular e causar alteração da sensibilidade na região anterior do tórax. O conhecimento da anatomia dos ramos nervosos ajuda a evitar problemas nessa região. .

Clavicle fractures - incidence of supraclavicular nerve injury.

OBJECTIVE: To analyze retrospectively 309 fractures in the clavicle and the relation with injury of the supraclavicular nerve after trauma. METHODS: It was analyzed 309 patients with 312 clavicle fractures. The Edinburgh classification was used. Four patients had fractures in the medial aspect of the clavicle, 33 in the lateral aspect and 272 in the diaphyseal aspect and three bilateral fractures. RESULTS: 255 patients were analyzed and five had paresthesia in the anterior aspect of the thorax. Four patients had type 2 B2 fracture and one type 2 B1 fracture. All patients showed spontaneous improvement, in the mean average of 3 months after the trauma. CONCLUSION: Clavicle fractures and/or shoulder surgeries can injure the lateral, intermediary or medial branches of the supraclavicular nerve and cause alteration of sensibility in the anterior aspect of the thorax. Knowledge of the anatomy of the nerve branches helps avoid problems in this region.

OBJETIVO: Analisar retrospectivamente 309 fraturas da clavícula e sua relação com a lesão do nervo supraclavicular após trauma. MÉTODOS: Foram analisados 309 pacientes com 312 fraturas da clavícula. Foi usada a classificação de Edinburgh. Quatro pacientes apresentavam fraturas da região medial da clavícula, 33 da região lateral, 272 da região diafisária e três com fraturas bilaterais. RESULTADOS: Foram analisados 255 pacientes e cinco apresentavam parestesia na região anterior do tórax. Quatro pacientes apresentaram fratura do tipo 2 B2 e um do tipo 2 B1. Todos os pacientes tiveram melhoria espontânea, em média de três meses após o trauma. CONCLUSÃO: Fraturas da clavícula e/ou cirurgias no ombro podem lesar os ramos lateral, intermediário ou medial do nervo supraclavicular e causar alteração da sensibilidade na região anterior do tórax. O conhecimento da anatomia dos ramos nervosos ajuda a evitar problemas nessa região.

Parafusos iliosacrais são realmente seguros? / Are iliosacral screws really safe?

OBJETIVO: Avaliar a segurança do uso de fixação iliosacral com parafusos em pacientes com lesão do anel pélvico. MÉTODOS: Trata-se de um trabalho restrospectivo analisando por tomografia computadorizada a topografia de 60 parafusos inseridos em 46 pacientes portadores de lesões do anel pélvico e operados no INTO-RJ, no período de 2006 e 2010. RESULTADOS: Em 21,7 por cento dos casos o parafuso encontrava-se fora do corredor de segurança descrito em literatura. Destes, 77 por cento estavam associados a uma redução insatisfatória do anel pélvico. Não houve casos de lesão neurovascular em nossa série. CONCLUSÃO: O uso de parafusos ílio-sacrais é uma técnica segura, pois mesmo em casos onde o parafuso não respeitou uma topografia ideal, não houve complicações significativas associadas a este método. A má redução do anel pélvico é um fator muito importante associado ao mau posicionamento dos parafusos.

OBJECTIVE: To evaluate the safety of iliosacral screw fixation in pelvic injuries. METHODS: A retrospective study based on computer tomography evaluation of screw topography and its relationship with the so called pelvic safe corridor. RESULTS: We evaluated the topography of 60 screws inserted in 46 patients sustaining pelvic injuries which were operated in the INTO from 2006 to 2010. In 21.7 percent of cases the screw was located outside the safe corridor. 77 percent of these misplaced screws were associated with an unsatisfactory reduction of the pelvic injury. CONCLUSIONS: In our study, a significant rate of misplaced screws in relationship with the safe corridor has been pointed out and in most of these cases an insufficient reduction of the pelvis was also identified. We conclude that iliosacral fixation is a safe adjuvant method for pelvic fixation since in our series even with a relative high incidence of misplacements, no neurovascular injuries have been recorded in association with this technique.

O Uso dos Parafusos de Apoio Transmedular nas Fraturas Diafisárias de Fêmur e Tíbia Tratadas com Haste Intramedular Bloqueada / The Use of Transmedullary Blocking screws In Femur And Tibia Fractures Treated With Intramedullary Blocking Nail

Avaliar e orientar os cirurgiões ortopédicos quanto às indicações de uso do parafuso de apoio transmedular nas fraturas diafisárias de fêmur e tíbia e avaliar radiograficamente o alinhamento do membro após redução da fratura

Osteonecrose e Pseudartrose após Osteossíntese de Fraturas do Colo Femoral em Adultos

Fraturas do colo femoral são cada vez mais prevalentes na população geral, devido principalmente ao envelhecimento da população e ao aumento da energia cinética envolvida nos acidentes automobilísticos. O tratamento com osteossíntese após redução da fratura é de escolha, principalmente em pacientes mais jovens e de maior demanda funcional