RESUMO
Advances from pharmacogenetics (PGx) have not been implemented into health care to the expected extent. One gap that will be addressed in this study is a lack of reporting on clinical validity and clinical utility of PGx-tests. A systematic review of current reporting in scientific literature was conducted on publications addressing PGx in the context of statins and muscle toxicity. Eighty-nine publications were included and information was selected on reported measures of effect, arguments, and accompanying conclusions. Most authors report associations to quantify the relationship between a genetic variation an outcome, such as adverse drug responses. Conclusions on the implementation of a PGx-test are generally based on these associations, without explicit mention of other measures relevant to evaluate the test's clinical validity and clinical utility. To gain insight in the clinical impact and select useful tests, additional outcomes are needed to estimate the clinical validity and utility, such as cost-effectiveness.
RESUMO
OBJECTIVE: In a previous discovery study, we identified seven potential screening markers for Down syndrome (DS). Here, we report on an extended study to validate the discriminative potential of these markers. METHODS: Concentrations of the seven analytes were measured using bead-based multiplexed immunoassays in maternal serum from 27 DS pregnancies and 27 matched controls. Control samples were matched to the cases by gestational age (exact day), maternal weight ( ± 5 kg), and maternal age ( ± 1 year) and by closest sample date. Prediction values were obtained for current screening markers [pregnancy-associated plasma protein A (PAPP-A), free beta human chorionic gonadotrophin (fß-hCG) and nuchal translucency (NT)] and seven markers identified before based on concentration fold ratios between DS and controls. Models were fitted based on data of the discovery study or this study and also tested on both datasets. RESULTS: A significantly higher fold ratio was only found for epidermal growth factor (EGF) (-1.96; p = 0.006). In the prediction model for the current dataset, EGF improved the detection rate (DR) of DS by 5.7% [at a fixed 5% false-positive rate (FPR)] when added to the currently used screening markers. CONCLUSIONS: Validation of previously identified biomarkers only confirmed EGF for further consideration as a DS screening marker. This underlines the importance of validating biomarkers; in this study, limiting the range of plausible biomarkers to only one suitable biomarker.
Assuntos
Síndrome de Down/sangue , Diagnóstico Pré-Natal/métodos , Proteômica/métodos , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Síndrome de Down/diagnóstico por imagem , Feminino , Humanos , Recém-Nascido , Valor Preditivo dos Testes , Gravidez , Primeiro Trimestre da Gravidez , Diagnóstico Pré-Natal/normas , Proteômica/normas , Reprodutibilidade dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , UltrassonografiaRESUMO
BACKGROUND: Peripheral blood mononuclear cells (PBMC) can be collected easily and repeatedly. Their potential use to reflect the individual's biological status is increasingly explored. Obesity is becoming the most common health problem of the 21st century, being dietary intake an important determinant of this pathology and numerous chronic health conditions. OBJECTIVE: The aim of this study is to identify PBMC genes involved in energy homeostasis, which could be good markers of overweight development. DESIGN: Using whole-genome microarray analysis, we evaluated the gene expression in PBMC of normoweight and diet-induced obese (cafeteria-fed) Wistar rats. RESULTS: Microarray analysis showed 566 genes differentially expressed between normoweight and cafeteria-fed rats. Of these, 35 genes were particularly involved in energy homeostasis. The gene with the biggest fold change was the 'solute carrier family 27 (fatty acid transporter), member 2' (slc27a2), which is implicated in lipid biosynthesis and fatty acid degradation. Scl27a2 was 33-fold overexpressed in cafeteria-fed rats compared with normoweight rats. This result was confirmed by quantitative PCR, although the overexpression was smaller (sixfold). Moreover, the increase in slc27a2 expression in PBMC of cafeteria-fed rats from 2 to 6 months of age paralleled the increase in body weight. CONCLUSION: The progressive overexpression of slc27a2 in PBMC of cafeteria-fed rats as the body weight increases suggests this gene as an early marker of overweight development related to the intake of a hyperlipidic diet.
Assuntos
Adiposidade/fisiologia , Coenzima A Ligases/metabolismo , Leucócitos Mononucleares/metabolismo , Obesidade/genética , Adiposidade/genética , Animais , Coenzima A Ligases/genética , Gorduras na Dieta/administração & dosagem , Metabolismo Energético/genética , Marcadores Genéticos/genética , Homeostase/genética , Masculino , Análise de Sequência com Séries de Oligonucleotídeos , Ratos , Ratos Wistar , Aumento de Peso/genéticaRESUMO
OBJECTIVES: To identify new discriminative biomarkers for Down syndrome (DS) pregnancies using a bead-based multiplexed immunoassay, and to use the newly identified biomarkers to construct a prediction model for non-invasive DS screening. METHODS: Maternal serum samples of 14 DS pregnancies and 15 matched controls were analyzed with a bead-based multiplexed immunoassay containing immunoassays for 90 different analytes. Potential biomarkers were selected on the basis of concentration fold ratios between DS and control samples. For these markers and the current screening markers (pregnancy-associated plasma protein-A, PAPP-A; free beta subunit of human chorion gonadotrophin (fbeta-hCG) and nuchal translucency) prediction values were obtained and used to calculate detection rates (DR) at a 5% false positive rate. RESULTS: Seven potential biomarkers of which the fold ratio exceeded 1.3 or -1.3 were selected for further analysis. All 14 DS cases in this study were detected using the combination of all currently used and newly identified markers. The modelled DR for all markers extrapolated to the general pregnant population was 82.5%, compared to a modelled DR of 56.2% for the current screening markers. CONCLUSION: This study demonstrates the possibility of improving the performance of the current first-trimester DS screening by addition of new biomarkers, which were identified using bead-based multiplexed immunoassays.