RESUMO
PURPOSE: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in liver ischemia/reperfusion injury in rats. METHODS: 25 male rats (Wistar) were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of liver ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during liver ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); Remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. RESULTS: Per+HSS group showed a lower degree of liver dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly improved liver function and reduced histological damage. CONCLUSION: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute liver injury induced by ischemia/reperfusion.
Assuntos
Precondicionamento Isquêmico/métodos , Fígado/irrigação sanguínea , Substâncias Protetoras/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Solução Salina Hipertônica/farmacologia , Animais , Modelos Animais de Doenças , Fígado/efeitos dos fármacos , Masculino , Distribuição Aleatória , Ratos Wistar , Albumina Sérica/análise , Estatísticas não ParamétricasRESUMO
Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in liver ischemia/reperfusion injury in rats. Methods: 25 male rats (Wistar) were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of liver ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during liver ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); Remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. Results: Per+HSS group showed a lower degree of liver dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly improved liver function and reduced histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute liver injury induced by ischemia/reperfusion.
Assuntos
Animais , Masculino , Solução Salina Hipertônica/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Precondicionamento Isquêmico/métodos , Substâncias Protetoras/farmacologia , Fígado/irrigação sanguínea , Albumina Sérica/análise , Distribuição Aleatória , Ratos Wistar , Estatísticas não Paramétricas , Modelos Animais de Doenças , Fígado/efeitos dos fármacosRESUMO
PURPOSE:: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. METHODS:: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. RESULTS:: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. CONCLUSION:: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.
Assuntos
Isquemia/prevenção & controle , Precondicionamento Isquêmico/métodos , Rim/irrigação sanguínea , Substâncias Protetoras/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Solução Salina Hipertônica/farmacologia , Animais , Nitrogênio da Ureia Sanguínea , Creatinina/sangue , Rim/química , Rim/patologia , Rim/fisiopatologia , Testes de Função Renal , Masculino , Necrose , Estresse Oxidativo , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Tiobarbitúricos/análise , Fatores de Tempo , Resultado do TratamentoRESUMO
Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.
