1.
Acta Crystallogr F Struct Biol Commun
; 71(Pt 4): 459-65, 2015 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-25849510
RESUMO
The latex of the common fig (Ficus carica) contains a mixture of at least five cysteine proteases commonly known as ficins (EC 3.4.22.3). Four of these proteases were purified to homogeneity and crystals were obtained in a variety of conditions. The four ficin (iso)forms appear in ten different crystal forms. All diffracted to better than 2.10â Å resolution and for each form at least one crystal form diffracted to 1.60â Å resolution or higher. Ficin (iso)forms B and C share a common crystal form, suggesting close sequence and structural similarity. The latter diffracted to a resolution of 1.20â Å and belonged to space group P3121 or P3221, with unit-cell parameters a = b = 88.9, c = 55.9â Å.