1.
ACS Biomater Sci Eng
; 5(5): 2117-2121, 2019 May 13.
Artigo
em Inglês
| MEDLINE
| ID: mdl-33405714
RESUMO
Low cell survival after syringe injection hampers the success of preclinical and clinical cell transplantation trials. During syringe injection, cells experience mechanical forces that lead to cell-membrane disruption and decreased viability. To improve cell survival, we designed rapidly assembling pentapeptides for injectable delivery (RAPID) hydrogels that shear-thin, protect cells from extensional flow, form fibers, and provide mechanical properties similar to native tissue. We found that 1.5 wt % RAPID hydrogels mitigate the damaging effects of extensional flow, resulting in significantly greater cell viability (of common laboratory cell lines, primary cells, and human cells) than cells injected in PBS.