[The effect of COVID-19 pandemic on attitude of internet-space surfers to professional activity of physician].

The content-analysis was applied to messages (created in April-May of 2018, 2020, 2021 and 2022) from Internet. The drastic increase of interest of audience to issue of medical care support and professional activity of physicians during period of increasing of morbidity of COVID-19 was established. The changing of basic sites for placement of content notably augmentation of mass media role was noticed. The increase of interest to research issue in audiences older than 60 years and in individuals with secondary special education was established. Furthermore, positive alteration of tonality of messages was noted. In 2018, on one positive message fell two negative messages. Starting from 2020, positive messages prevail over negative messages: 2020 - in two times, 2021 - 2.1 times, 2022 - 4.6 times. In absolute values, the number of messages of positive tonality increased up to 9.8 times in 2022 as compared to 2018. Starting from 2020, in the word cloud appeared words "gratitude" and "thank you".

[The participation of religious organizations in formation of population attitude to transplantation of organs and tissues].

The article discusses issue of possibility of influence of religious organizations on the attitude of population to issues of human organ transplantation. The attitude of followers of different religions to key issues of bioethics related to organ donation and transplantation is analyzed. It is concluded that there are no definite prohibitions on implementing this kind of medical intervention in modern religious regulations. The study of results of questionnaire survey of two groups of respondents revealed certain part of population, whose decision on the issues of posthumous organ donation would be influenced by the opinion of representative of religious organization. It is established that every tenth respondent appealed to the Orthodox priest when making decision to sign consent paper concerning posthumous exempt of organ from relatives. It is concluded that it is possible to affect the formation in certain part of population understanding of need to support organ donation and transplantation in case of active work in this direction of representatives of religious organizations.

[The Attitude of Physicians of Donor Bases to Problems of Transplantation of Human Organs and Tissues].

The article considers social aspects of transplantology on the basis of analysis of data of questionnaire survey of 266 physicians of donor bases of the Federal Center implementing transplantation of human organs and tissues. The respondents were representatives of the most prevalent medical professions and ll age gender groups. According to study results, physicians are aware of importance of development of organs and tissues transplantation in the region though take a very passive standing related to informing transplantation specialists about availability of a potential donor. The involvement of physicians in the transplantation program in the first place depends on position of administration of medical institution. At the same time, 44,3±3,1% of physicians are aware of such the most important normative legal principle regulating possibility of postmortem intake of organs as consent presumption. Less than 18,6±2,4% of physicians support this standard. Half of the respondents are ready, in case of their instant death, to become donors of organs and are ready too in case of such a proposal to sign consent to possible postmortem donorship. Therefore, one of conditions of development of organs and tissues transplantation in the region is implementation of explanatory work with medical community targeted to increasing of motivation of personnel of donor bases to active interaction with transplantology specialists.

[THE MOTIVATION OF MEDICAL PERSONNEL OF MULTIFIELD HOSPITAL TO WORKING OVER STANDARDS OF SINGLE JOB POSITION].

The article considers results of study concerning prevalence of physicians working over standards of single job position in multifield hospital, including factors motivating to extra job. The research purpose was to analyze impact of gender age structure of medical personnel of multfield public medical organization on motivation of physicians to work over standards of single job position. The objectives consisted of analyzing prevalence of over standard work of medical personnel of multifield public medical organization with consideration of social structure and studying factors motivating physicians to work over standards of single job position. The study was carried out on the basis of questionnaire survey of physicians. The results of study testify higher degree of prevalence of working over standards of single job position in modern health care. So, 64.8 ± 3.6% of respondents work subject to conditions of internal and/or external moonlighting. Moreover; one third of physicians enlisted to extra job. Among physicians males more often than females work subject to conditions of moonlighting, perform extra job, enlist to working during days off. The specialists of age group from 35 to 54 years work subject to conditions of external and internal moonlighting more often than younger physicians. Among physicians, the most significant cause of working subject to conditions of moonlighting is additional earnings. At that, every fifth physician works over standards of job position with purpose to increase one's professional competence. The study results permit concluding that aspects of social structure of modern medical staff significantly impact motivation and hence possibility of enlisting workers to work over standards of single job position.

[THE OPTIMIZATION OF THE ACTIVITY OF ORGANS OF FEDERAL SERVICE FOR SUPERVISION OF CONSUMER RIGHTS PROTECTION AND HUMAN WELFARE IN THE SVERDLOVSK REGION].

In the Sverdlovsk region there have developed and implemented methodological approaches to the optimization oj the activity of the Directorate and the Centre directed to the improvement of the sanitary and epidemiological surveillance and in the sphere of the protection of the rights of consumers in the framework of the development of an comprehensive regional system of risk management for the population's health in the Sverdlovsk region.

[The forensic medical identification of the members of the Demidov family].

The objective of the present study was to elucidate the genetic profiles of the biological materials taken from four graves in the Demidov family vault in order to establish kinship between its members. According to the archival documents, two graves contained the remains of Pyotr Grigor'evich Demidov, an adjutant-general for the emperor Aleksandr II, and his wife Elizaveta Nikolaevna Demidova (Bezobrazova). Also, it was supposed that two other graves contained the remains of Grigory Petrovich Demidov and Ekaterina Petrovna Demidova (married name princess Kudasheva), the son and the daughter of P.G. Demidov and E.N. Demidova. The bodies remained in the half-ruined crypt during approximately 150 years under conditions of enhanced humidity and seasonal temperature fluctuations which made their bone tissue virtually unsuitable for the genetic analysis. Genotyping was performed with the use of standard AmpF/STR Identifiler-TM and AmpF/STR Yfiler-TM kits ("Applied Biosystems", USA). As a result of the study, the skeletal remains of the boy from grave No2 were identified as actually belonging to the son of P.G. Demidov and E.N. Demidova with a probability of no less than 99.999999998%. whereas the girl buried in grave No4, was not the daughter of these parents.

[The socio-hygienic monitoring as an integral system for health risk assessment and risk management at the regional level].

The information and analytical framework for the introduction of health risk assessment and risk management methodologies in the Sverdlovsk Region is the system of socio-hygienic monitoring. Techniques of risk management that take into account the choice of most cost-effective and efficient actions for improvement of the sanitary and epidemiologic situation at the level of the region, municipality, or a business entity of the Russian Federation, have been developed and proposed. To assess the efficiency of planning and activities for health risk management common method approaches and economic methods of "cost-effectiveness" and "cost-benefit" analyses provided in method recommendations and introduced in the Russian Federation are applied.

[Organizational support of investigation of Legionnaires' disease outbreak in town Verkhnyaya Pyshma].

Materials on organizational support of preventive and antiepidemic measures during outbreak of Legionnaires' disease in town Verkhnyaya Pyshma (Sverdlovsk region) in July-August 2007 are presented. Thanks to collaborative efforts of Federal Service for Surveillance for Protection of Consumers Rights and Human Welfare, Ministry of Health of Sverdlovsk region, health-care services in Verkhnyaya Pyshma, near located municipalities, and others it was able to establish in short term the hospital for treatment, identify source of the infection and factor of its transmission, and terminate the outbreak. Operative implementation of the mentioned measures both allowed to provide good quality medical care to all patients and to prevent occurrence of such situations on other towns of Sverdlovsk region.

[The forces and expedients of institutions of Federal Service for Surveillance for Protection of Consumers Rights and Human Welfare in Sverdlovsk region in elimination of focus of Legionnaires' disease].

Organizational issues as well as composition of forces and tools involved in elimination of focus of Legionnaires' disease were reviewed. Role of institutions of Federal Service for Surveillance for Protection of Consumers Rights and Human Welfare and their special units in general system of measures for elimination of the disease's focus was reflected.

[Influence of serum IgG antibodies to Chlamydia trachomatis on the functional activity of complement components].

The immunoenzyme analysis and the method for the determination of IgG-containing immune complexes, carrying C1q component of the complement, were developed. In human blood sera the functional activity of components C3, complex C1r2s2, the content of C1 inhibitor and complement-activating immune complexes were determined. The comparative analysis of the activity of components C3 and C1r2s2, as well as between the content of C1 inhibitor and the activity of complex C1r2s2 for seropositive and seronegative sera, was made. Pronounced correlation for seropositive sera was observed. In addition, for seropositive sera correlation between an increase in IgG immune complexes and a drop in the functional activity of complex C1r2s2, as well as a drop in the functional activity of complex C1r2s2 and a growth in the titers of IgG antibodies to Chlamydia trachomatis, were established. The decreased functional activity of key complement components, simultaneously with the presence of complement-activating immune complexes and high titers of specific antibodies could be the diagnostic criteria of carrier state.

[Determination of proteinase activity of complement system by immunoenzyme methods]. / Opredelenie aktivnosti proteinaz sistemy komplimenta immunofermentnymi metodami.

Modern ELISA for determination of functional activity of component C2 and factors B and D, proteinases of a complement system, and component C3, substrate C3-convertases, key complex enzymes of the complement, have been developed. Essential feature of C3-convertases classical (C4bC2a) and alternative (C3bBb) pathways of the complement activation is that their substrate C3 after proteolytic cleavage is converted into C3b, carrying on the surface thioester covalent bond linking C3b with nucleophilic acceptors that results in immobilization of this proteolytic product near the activating enzyme. Cascade character of an activation of complement system allows to create artificial deficit of separate components in the experimental system and to determine (by ELISA) covalently immobilized component C3 during activation, and also to determine functional activity of any of pre-exhausted components. Use of such approach resulted in the development of the ELISA systems suitable for determination of functional activity of component C2 of classical pathway and factors B and D of the alternative pathway by testing quantity of the immobilized C3b at excess C3. The developed methods allow to investigate mechanisms of functioning of complement, inhibition of the cascade activation by endogenic and exogenous inhibitors, and also to find functional deficiency of components in serum and other biological fluids.

Investigation of Mueller matrices of anisotropic nonhomogeneous layers in application to an optical model of the cornea.

We consider a system of anisotropic layers as an optical model of the eye cornea. Effective refraction indices for normally incident light are calculated with the assumption that each layer consists of closely packed uniform cylinders (fibrils). Jones matrix formalism is used to describe light propagation through the cornea. We calculate the Jones matrices from the experimentally measured Mueller matrices. Two algorithms are used for this purpose. The experiments have shown that ~20% of cornea area studied had the structure well described by the helical model proposed.