Improving agronomic water use efficiency in tomato by rootstock-mediated hormonal regulation of leaf biomass.

Water availability is the most important factor limiting food production, thus developing new scientific strategies to allow crops to more efficiently use water could be crucial in a world with a growing population. Tomato is a highly water consuming crop and improving its water use efficiency (WUE) implies positive economic and environmental effects. This work aimed to study and exploit root-derived hormonal traits to improve WUE in tomato by grafting on selected rootstocks. Firstly, root-related hormonal parameters associated to WUE were identified in a population of recombinant inbred lines (RILs) derived from the wild tomato species Solanum pimpinellifolium. A principal component analysis (PCA) revealed that some hormonal traits were associated with productivity (plant biomass and photosynthesis) and WUE in the RIL population. Leaf ABA concentration was associated to the first component (PC1) of the PCA, which explained a 60% of the variance in WUE, while the ethylene precursor ACC and the ratio ACC/ABA were also associated to PC1 but in the opposite direction. Secondly, we selected RILs according to their extreme biomass (high, B, low, b) and water use (high, W, low, w), and studied the differential effect of shoot and root on WUE by reciprocal grafting. In absence of any imposed stress, there were no rootstock effects on vegetative shoot growth and water relations. Finally, we exploited the previously identified root-related hormonal traits by grafting a commercial tomato variety onto the selected RILs to improve WUE. Interestingly, rootstocks that induced low biomass and water use, 'bw', improved fruit yield and WUE (defined as fruit yield/water use) by up to 40% compared to self-grafted plants. Although other hormonal factors appear implicated in this response, xylem ACC concentration seems an important root-derived trait that inhibits leaf growth but does not limit fruit yield. Thus tomato WUE can be improved exploiting rootstock-derived hormonal signals which control leaf growth.

Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.).

Leaf senescence is one of the most limiting factors to plant productivity under salinity. Both the accumulation of specific toxic ions (e.g. Na+) and changes in leaf hormone relations are involved in the regulation of this process. Tomato plants (Solanum lycopersicum L. cv Moneymaker) were cultivated for 3 weeks under high salinity (100 mM NaCl) and leaf senescence-related parameters were studied during leaf development in relation to Na+ and K+ contents and changes in abscisic acid (ABA), cytokinins, the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and the auxin indole-3-acetic acid (IAA). Na+ accumulated to a similar extent in both leaves 4 and 5 (numbering from the base of the plant) and more quickly during the third week, while concurrently K+ contents sharply decreased. However, photosystem II efficiency, measured as the F(v)/F(m) ratio, decreased from the second week of salinization in leaf 4 but only at the end of the third week in the younger leaf 5. In the prematurely senescent leaf 4, ABA content increased linearly while IAA strongly decreased with salinization time. Although zeatin (Z) levels were scarcely affected by salinity, zeatin-riboside (ZR) and the total cytokinin content (Z+ZR) progressively decreased by 50% from the imposition of the stress. ACC was the only hormonal compound that increased in leaf tissue coincident with the onset of oxidative damage and the decline in chlorophyll fluorescence, and prior to massive Na+ accumulation. Indeed, (Z+ZR) and ACC contents and their ratio (Z+ZR/ACC) were the hormonal parameters best correlated with the onset and progression of leaf senescence. The influence of different hormonal changes on salt-induced leaf senescence is discussed.

Robotized thermal and chlorophyll fluorescence imaging of pepper mild mottle virus infection in Nicotiana benthamiana.

After infecting a susceptible host, pathogens spread throughout the plant. Depending on pathogen type and strain, the severity of symptoms varies greatly. In the case of pepper mild mottle virus (PMMoV) infection in Nicotiana benthamiana, newly developing leaves display visual symptoms (symptomatic leaves). In this study, two PMMoV strains were used, the Spanish strain (PMMoV-S) being more virulent than the Italian strain (PMMoV-I). Plants infected with PMMoV-I could recover from the virus-induced symptoms. Leaves that were fully developed at the start of PMMoV infection remained symptomless. In these asymptomatic leaves, an increase in temperature, initiating from the tissue adjacent to the main veins, was observed 7 d before the Chl fluorescence pattern changed. Virus immunolocalization on tissue prints matched well with the concomitant pattern of Chl fluorescence increase. The temperature increase, associated with the veins, was shown to be related to stomatal closure. Upon PMMoV-I infection, the appearance of thermal and Chl fluorescence symptoms as well as virus accumulation were delayed by 3 d compared with PMMoV-S-induced symptoms. The temperature increase of whole symptomatic leaves was also correlated with a decrease in stomatal aperture. In contrast to the persistent increase in symptomatic leaf temperature observed during PMMoV-S infection, the temperature of symptomatic leaves of PMMoV-I-infected plants decreased gradually during recovery. We propose that the earliest temperature increase is caused by a systemic plant response to the virus infection, involving the control of water loss. In conclusion, thermography has potential as an early reporter of an ongoing compatible infection process.