Erosive Tooth Wear, Wine Intake, and Genetic Variation in COMT and MMP2.

INTRODUCTION: Erosive tooth wear (ETW) is a multifactorial condition of increasing prevalence in the younger population. This study aimed to explore the association between different ETW phenotypes with MMP2 and COMT single-nucleotide variants, and selected environmental factors. METHODS: Saliva samples, erosive wear and dental caries experience data, and dietary/behavioral information from 16-18-year-old patients (n= 747) were used. Genotypes were obtained and phenotypes were further analyzed considering diet and behavioral data, using logistic regression as implemented in PLINK, with an alpha of 0.05. RESULTS: When comparing individuals' ETW-free with those with mild ETW, an association was found with COMT rs6269 (p = 0.02). The comparison between ETW-free individuals with individuals with severe ETW also showed an association with COMT rs6269 under the recessive model (p = 0.03). Logistic regression showed that in the presence of less common alleles of MMP2 rs9923304 and COMT rs6269, ETW were more likely to occur when individuals drank wine. The GG genotype of COMT rs6269 was associated with the presence of lower (p = 0.02) and higher (p = 0.02) caries experience when individuals with ETW only in enamel were compared with individuals with ETW involving dentin. CONCLUSION: The results support a role of genes in ETW, with wine consumption being identified as a significant modulator, suggesting that gene-environment interactions may contribute to the development of erosive tooth wear.

Bone age and dental late effects in childhood cancer survivors: Radiographic findings in a Brazilian sample.

BACKGROUND: Changes in bone age and tooth development are late side effects of cancer therapy and can be identified by imaging examination. AIM: To evaluate the late effects of antineoplastic treatment on bone age and dental development in childhood cancer survivors. DESIGN: This is a retrospective case-control study on paediatric cancer survivors of both sexes who underwent antineoplastic treatment with 5-15 years of survival. Carpal radiographs were assessed for bone age and growth curve, and panoramic radiographs were used to evaluate dental development and alterations. Carpal radiographs were analyzed using the Greulich and Pyle inspection method, and the Martins and Sakima method was used to analyze the growth curve. All tests were applied with a confidence level of 95%. RESULTS: The study and control groups comprised 28 and 56 patients, respectively. There was no significant difference in bone age and growth curve between the study and control groups. Nonetheless, when sex was compared to chronological and bone ages, there was a significant difference in bone age (p = 0.019) and an underestimation in both groups and sexes in the Greulich and Pyle method. As to late dental effects, dental agenesia, microdontia, gyroversion, and unerupted teeth were found. Dental shape alterations mainly involve the root region. CONCLUSION: Close multidisciplinary collaboration is necessary during the follow-up period of young patients who have survived cancer.

Efficacy of a high concentration of nano silver fluoride in preventing caries: A randomized controlled trial.

Background: To investigate the effectiveness of a novel agent containing Nano Silver Fluoride 1500 (NSF 1500) and chitosan to inactivate carious lesions in children. Material and Methods: The study included eighty children. While both groups had fluoride dentifrice applied to their teeth, only the experimental group received treatment with the NSF 1500-ppm solution. The first and sixth-month interval examinations were conducted by two calibrated dentists (k = 0.85). Results: The NSF 1500 group had 69.2% of their teeth with arrested decay, while the control group had 24.1%. The difference was statistically significant (p 0.001), with a preventive fraction of 59.4%. The number needed to treat (NNT) was approximately two. The NSF 1500 formulation was more effective than toothbrushing alone with fluoridated dentifrice in preventing dental caries. Conclusions: The effectiveness of NSF 1500 is determined by the size and depth of the dental cavity. Its ability to arrest caries lesions was comparable to previously tested products, NSF 400 and NSF 600. Key words:Preventive dentistry, dental caries, nanoparticles.

An exploratory study of children with caries and its relationship to SARS-CoV-2.

This exploratory study investigated whether children with dental decay were more likely to have COVID-19 than those without caries. The children underwent dental inspection and blood collection for detection of SARS-CoV-2 antibodies. Fifty-four children aged 6 to 9 years participated in the survey, which was conducted between March and June 2020 in the municipality of Ipojuca, Pernambuco, Brazil. The diagnosis of caries was performed using the dmft and DMFT indices. Parents reported signs and symptoms of sickness in their children during this period. The serology test aimed to verify the immune response of the children to coronavirus by detecting IgM/IgG antibodies. Statistical analyses were performed at P < 0.05. The majority of the children presented caries (68.5%). Of the nine children who tested positive for COVID-19 (16.7%), eight presented IgG antibodies to the virus, and only one had IgG and IgM antibodies to SARS-CoV2. Children who tested positive for SARS-CoV-2 had a higher percentage of caries lesions than those who tested negative for SARS-CoV-2 (77.8% vs 65.9%), but this difference was not statistically significant.

Sense of Coherence and Oral Health in Children and Adolescents: a Scoping Review.

PURPOSE: The purpose of this study was to review the published studies that address the sense of coherence and its relationship with the oral health of children and adolescents. METHODS: This scoping review was structured according to the review method proposed by the Joanna Brigs Institute and conducted in accordance with PRISMA-ScR guidelines. The research was carried out in the following databases: Medline/ Pubmed®, Lilacs®, Scopus©, Cochrane©, Web of Science©, and Embase©. RESULTS: In this search, 358 studies were found, seven in Cochrane, 90 in PubMed®, three in Lilacs®, 101 in Web of Science©, 80 in Scopus©, and 77 in Embase©, totaling 24 publications. The studies were published in nine countries, most of them cross-sectional studies. CONCLUSIONS: Most studies have shown that a high sense of coherence (SOC) of both the caregiver and the child/adolescent is related to better oral health behaviors and a lower caries index. No conclusive information was observed on the relationship between SOC and periodontal diseases.

An exploratory study of children with caries and its relationship to SARS-CoV-2

Abstract This exploratory study investigated whether children with dental decay were more likely to have COVID-19 than those without caries. The children underwent dental inspection and blood collection for detection of SARS-CoV-2 antibodies. Fifty-four children aged 6 to 9 years participated in the survey, which was conducted between March and June 2020 in the municipality of Ipojuca, Pernambuco, Brazil. The diagnosis of caries was performed using the dmft and DMFT indices. Parents reported signs and symptoms of sickness in their children during this period. The serology test aimed to verify the immune response of the children to coronavirus by detecting IgM/IgG antibodies. Statistical analyses were performed at P < 0.05. The majority of the children presented caries (68.5%). Of the nine children who tested positive for COVID-19 (16.7%), eight presented IgG antibodies to the virus, and only one had IgG and IgM antibodies to SARS-CoV2. Children who tested positive for SARS-CoV-2 had a higher percentage of caries lesions than those who tested negative for SARS-CoV-2 (77.8% vs 65.9%), but this difference was not statistically significant.

Oral manifestations in hospitalized children with COVID-19.

As the pandemic progressed, the incidence of viruses among children also increased. This study investigates the presence of oral lesions in hospitalized children by analyzing data collected from medical records of infants seen at the pediatric Infectious disease unit at the General Hospital of the University of Pernambuco, Recife, Brazil, from March to August 2020. This study includes children aged 0 to 12 years diagnosed with severe symptoms of COVID-19. The data describe the frequencies and percentages of categorical variables, expressed as mean, median, and standard deviation. The chi-square test evaluated the association of oral manifestations according to the presence of comorbidities. Of 89 children, 20.2% had oral manifestations, and mucositis was the most prevalent lesion (12.4%). Of the 18 children with oral manifestations, 12 did not present comorbidities, but 7.9% had multisystem inflammatory syndrome and 5.6% had Kawasaki disease. Results show that children with oral lesions had longer hospital stays. These findings indicate the need for further studies to clarify the relationship between the oral manifestations of COVID-19 in pediatric patients for screening of the virus by dentists.

Sense of coherence and quality of life in adolescents with heart disease.

OBJECTIVE: The aim of this study was to assess the association between the Sense of Coherence (SoC) and Quality of Life (QoL) in adolescents with heart disease. METHODS: A cross-sectional study was carried out with 164 adolescents with congenital or valvular heart disease, aged between 10 and 18 years, treated in a referral center in the city of Recife - Brazil. The information collected contains census data, type of heart disease, economic status identified according to the Brazilian Criteria for Economic Classification (ABEP), as well as an evaluation of the SoC and the QoL. RESULTS: The SoC (50.09) and QoL (72.23) exhibited high average scores. The SoC was positively correlated with all dimensions of the QoL scale (p<0.001). The social and school dimensions, respectively, presented the highest and lowest scores. The linear regression analysis revealed that the SoC influenced the school and emotional dimensions. CONCLUSIONS: This study demonstrates that the SoC is a protective factor in the life of adolescents. This factor helps on the improvement of perception of QoL and on successfully dealing with daily adversities and chronic stress.

Viral symptoms in children and SARS-COV-2: information for pediatric dentists for the control of transmission.

The new coronavi rus, which has spread worldwide, has spiraled out of control in Brazil. The number of infected children has increased, and more Infants Special Care Units are needed to prevent deaths. This study aims to report the most common signs and symptoms in children infected by seasonal respiratory viruses and those infected by COVID-19. This knowledge is essential to educate pediatric dentists, who may contribute to identifying the difference in symptoms and notify the cases, thus preventing the spread of COVID-19. This study was carried out in a Family Health Center of Ipojuca, state of Pernambuco, Brazil, and included 54 children seen for dental emergency care. The parents provided information about the signs and symptoms of their children' s health conditions during the lockdown from March to July 2020. All children had a serological test to detect any exposure to the virus. Kolmogorov-Smirnov and Mann-Whitney tests were used to assess the distribution of the data and compare the quantitative variables between the groups. Among the study participants, 16.7% tested positive for COVID-19. The most prevalent symptoms were headache (38.9%), sneezing (35.2%), and fever (20.4%); six out of nine children with a positive test had symptoms after infection of an adult in the family. Children infected with COVID-19 showed similar clinical signs to those with negative results. Pediatric dentists should acquire knowledge to report on sick children and prevent the spread of the disease.

Viral symptoms in children and SARS-COV-2: information for pediatric dentists for the control of transmission

Abstract: The new coronavi rus, which has spread worldwide, has spiraled out of control in Brazil. The number of infected children has increased, and more Infants Special Care Units are needed to prevent deaths. This study aims to report the most common signs and symptoms in children infected by seasonal respiratory viruses and those infected by COVID-19. This knowledge is essential to educate pediatric dentists, who may contribute to identifying the difference in symptoms and notify the cases, thus preventing the spread of COVID-19. This study was carried out in a Family Health Center of Ipojuca, state of Pernambuco, Brazil, and included 54 children seen for dental emergency care. The parents provided information about the signs and symptoms of their children' s health conditions during the lockdown from March to July 2020. All children had a serological test to detect any exposure to the virus. Kolmogorov-Smirnov and Mann-Whitney tests were used to assess the distribution of the data and compare the quantitative variables between the groups. Among the study participants, 16.7% tested positive for COVID-19. The most prevalent symptoms were headache (38.9%), sneezing (35.2%), and fever (20.4%); six out of nine children with a positive test had symptoms after infection of an adult in the family. Children infected with COVID-19 showed similar clinical signs to those with negative results. Pediatric dentists should acquire knowledge to report on sick children and prevent the spread of the disease.

Oral manifestations in hospitalized children with COVID-19

Abstract As the pandemic progressed, the incidence of viruses among children also increased. This study investigates the presence of oral lesions in hospitalized children by analyzing data collected from medical records of infants seen at the pediatric Infectious disease unit at the General Hospital of the University of Pernambuco, Recife, Brazil, from March to August 2020. This study includes children aged 0 to 12 years diagnosed with severe symptoms of COVID-19. The data describe the frequencies and percentages of categorical variables, expressed as mean, median, and standard deviation. The chi-square test evaluated the association of oral manifestations according to the presence of comorbidities. Of 89 children, 20.2% had oral manifestations, and mucositis was the most prevalent lesion (12.4%). Of the 18 children with oral manifestations, 12 did not present comorbidities, but 7.9% had multisystem inflammatory syndrome and 5.6% had Kawasaki disease. Results show that children with oral lesions had longer hospital stays. These findings indicate the need for further studies to clarify the relationship between the oral manifestations of COVID-19 in pediatric patients for screening of the virus by dentists.

Sense of coherence and quality of life in adolescents with heart disease / Senso de coerência e qualidade de vida em adolescentes com doenças cardíacas

Abstract Objective: The aim of this study was to assess the association between the Sense of Coherence (SoC) and Quality of Life (QoL) in adolescents with heart disease. Methods: A cross-sectional study was carried out with 164 adolescents with congenital or valvular heart disease, aged between 10 and 18 years, treated in a referral center in the city of Recife - Brazil. The information collected contains census data, type of heart disease, economic status identified according to the Brazilian Criteria for Economic Classification (ABEP), as well as an evaluation of the SoC and the QoL. Results: The SoC (50.09) and QoL (72.23) exhibited high average scores. The SoC was positively correlated with all dimensions of the QoL scale (p<0.001). The social and school dimensions, respectively, presented the highest and lowest scores. The linear regression analysis revealed that the SoC influenced the school and emotional dimensions. Conclusions: This study demonstrates that the SoC is a protective factor in the life of adolescents. This factor helps on the improvement of perception of QoL and on successfully dealing with daily adversities and chronic stress.

Resumo Objetivo: Avaliar a associação entre Senso de Coerência (SOC) e Qualidade de Vida (QV) em adolescentes cardiopatas. Métodos: Foi realizado um estudo transversal com 164 adolescentes portadores de cardiopatias congênitas ou valvares, com idade entre 10 e 18 anos, atendidos em um Centro de Referência na cidade de Recife - Brasil. As informações coletadas contêm dados censitários, tipo de cardiopatia, situação econômica identificada de acordo com os Critérios Brasileiros de Classificação Econômica (ABEP), além de uma avaliação do Senso de Coerência e da Qualidade de Vida. Resultados: Tanto o Sentido de Coerência (50,09) quanto a Qualidade de Vida (72,23) exibiram valores médios elevados. Senso de Coerência foi positivamente correlacionado com todas as dimensões da Qualidade de Vida (p<0,001). As dimensões social e escolar foram as pontuações mais altas e mais baixas, respectivamente. A análise de regressão linear revelou que o Sentido de Coerência influenciou as dimensões escolar e emocional. Conclusões: Este estudo demonstra que o Sentido de Coerência é um fator de proteção na vida dos adolescentes. Esse fator auxilia na melhora da percepção de Qualidade de Vida e no enfrentamento com sucesso das adversidades do dia a dia e do estresse crônico.

Nano-silver Fluoride at Higher Concentration for Caries Arrest in Primary Molars: A Randomized Controlled Trial.

AIM AND OBJECTIVE: To compare the arresting caries effectiveness of two different silver nanoparticle (AgNP) concentrations of nano-silver fluoride (NSF), namely 400 and 600 ppm. The hypothesis is that in posterior primary teeth with occlusal and approximal active dentin carious lesions, NSF 600's effectiveness will be higher than that of the NSF 400 solution over a 6-month follow-up period. MATERIALS AND METHODS: This was a double-blind randomized clinical trial (RCT) conducted in the city of Recife, Brazil. A total of 337 children aged 5-7 years who attended the University of Pernambuco Dental School's clinics were examined. A single-blinded investigator conducted the examinations and treatment of the children. After baseline examination and recording of the dmft index, children were allocated to one of two study groups (NSF 600-intervention and NSF 400-positive control). In both groups, each tooth received two drops of NSF and treatments were performed only once in 6 months. The follow-up examinations were visual and tactile, performed in 30, 90, and 180 days to determine the activity of caries. The carious lesions that were not arrested in 30 days were recorded and referred for restorative treatment. RESULTS: The NSF 600 showed higher rate of success in arresting caries (72.7%, p = 0.025) compared with NSF 400 (56.5%). CONCLUSION: The higher rate of success of NSF 600 can be explained by the higher concentration of AgNPs. CLINICAL SIGNIFICANCE: Nano-silver fluoride has emerged as an excellent alternative to silver diamine fluoride (SDF), as it adds a high antibacterial effect to better esthetic results. Treatment is simple, non-invasive, and incurs low cost. It is ideal for use in community-based programs to increase the access to dental care without staining teeth black like other silver products. HOW TO CITE THIS ARTICLE: Arnaud M, Junior PCM, Lima MGS, et al. Nano-silver Fluoride at Higher Concentration for Caries Arrest in Primary Molars: A Randomized Controlled Trial. Int J Clin Pediatr Dent 2021;14(2):207-211.

Evaluation of the lingual frenulum in newborns using two protocols and its association with breastfeeding / Avaliação do frênulo lingual em recém-nascidos com dois protocolos e sua relação com o aleitamento materno

Abstract Objective: To characterize the lingual frenulum of full-term newborns using two different protocols and to assess the association of the lingual frenulum with breastfeeding. Methods: This non-probabilistic sample consisted of 449 mother/baby binomials. For the anatomo-functional evaluation of the frenulum, the Neonatal Tongue Screening Test and the Bristol Tongue Assessment Tool were used for the evaluation of the lingual frenulum. Breastfeeding was evaluated using the protocol proposed by UNICEF. Scores were created (good, fair, poor) to evaluate every aspect of the breastfeeding to be observed. The results were analyzed through descriptive and inferential statistics and association tests (Pearson's chi-squared and Fisher's exact test). Results: The study showed that 14 babies had a lingual frenulum alteration, of whom three had difficulties during suction, requiring frenotomy in the first week of life, whereas 11 had no difficulties during breastfeeding. Regarding the breastfeeding evaluation, 410 mother/baby binomials had good, 36 regular, and three had bad scores. There was a statistically significant association between the tongue-tie test protocol and breastfeeding (p = 0.028) and between the Bristol Tongue Assessment Tool protocol and breastfeeding (p = 0.028). Conclusion: Alterations in the lingual frenulum are associated with interferences in the quality of breastfeeding and thus, evaluation of the lingual frenulum in newborns is important.

Resumo Objetivo: Caracterizar o frênulo lingual de recém-nascidos a termo, utilizando dois protocolos diferentes e verificar a relação do frênulo lingual com o aleitamento materno. Método: A amostra não probabilística foi constituída por 449 binômios mãe/bebê. Para a avaliação anatomofuncional do frênulo, utilizou-se o protocolo de avaliação do frênulo lingual para bebês "Teste da Linguinha" e o Bristol Tongue Assessment Tool. A mamada foi avaliada com o protocolo proposto pelo UNICEF. Foram criados escores (bom, regular, ruim) para avaliar cada aspecto da mamada a ser observada. Os resultados foram analisados através de estatísticas descritivas e inferenciais e testes de associação (Qui-quadrado de Pearson e Exato de Fisher). Resultados: O estudo mostrou que 14 bebês apresentaram alteração de frênulo lingual, nos quais três com dificuldade durante a sucção, necessitando de frenotomia na primeira semana de vida e 11 sem dificuldades durante a amamentação. Quanto à avaliação da mamada, 410 binômios mãe/bebê apresentaram o escore bom, 36 regular e 3 ruim. Houve associação estatisticamente significativa entre o protocolo "Teste da Linguinha" e amamentação (p = 0,028) e entre o protocolo Bristol Tongue Assessment Tool e amamentação (p = 0,028). Conclusão: Alterações no frênulo lingual estão associadas a interferências na qualidade da amamentação, sendo importante a avaliação do frênulo lingual em recém-nascidos.

Caries experience in children and adolescents with Down Syndrome: A systematic review and meta-analysis.

AIM: This study aimed to perform a systematic review and meta-analysis comparing the prevalence of dental caries in non-syndromic children with children and adolescents with Down syndrome (DS). METHODS: The strategy included a search for articles in electronic databases (MEDLINE via PubMed, Cochrane Library, LILACS, and SciELO). The methods for assessment included the modified Newcastle-Ottawa scale, the methodological quality for cross-sectional studies, and a random-effects meta-analysis model. By obtaining the odds ratio, the tests of heterogeneity and sensitivity were performed. This study includes unpublished data from the cross-sectional study by Paiva et al. (2018), which was conducted in the city of Recife/PE. RESULTS: Twelve cross- section studies met the eligibility criteria and were included in the meta-analysis. Results indicated that children and adolescents with DS had lower caries experience when compared to the non-syndromic ones (95 % CI: 0.22-0.84). The results indicated that the seven studies were statistically significant (p < 0.00001). The risk of bias remained high given that the majority of the studies were cross-sectional reports. CONCLUSION: The results of this study fill the lack of knowledge and indicated that there is scientific evidence to suggest that children and adolescents with Down Syndrome have fewer caries than the non-syndromic group.

Prevalence of sleep-disordered breathing and associations with malocclusion in children.

STUDY OBJECTIVES: This study aimed to determine the prevalence of sleep-disordered breathing (SDB) and its association with malocclusion among children in Recife, Brazil. METHODS: This study included 390 children aged 7 to 8 years. The data comprised the measurement of body mass, orthodontic examination, and parental information required by the Sleep Disturbance Scale for Children. The statistics tools used were Pearson's chi-square test and the Lemeshow test. RESULTS: Positively screened for SDB was found in 33.3% of the children, and the association with overjet was P = .007 (odds ratio [OR], 95%, confidence interval [CI]: 1.93). The association with anterior open bite was P = .008 (OR, 95% CI: 2.03), and the association with posterior crossbite was P = .001 (OR, 95% CI: 2.89). This report was unable to indicate an association between body mass index and SDB. The multivariate logistic regression model revealed that the anterior open bite (P = .002; OR, 95% CI: 2.34) and posterior crossbite (P = .014; OR, 95% CI: 2.79) had an association with positively screened for SDB. CONCLUSIONS: The results of this study indicated that the prevalence of SDB was high and highly associated with malocclusion. Since posterior crossbite and anterior open bite were associated with positively screened for SDB, early diagnosis and intervention may prevent and minimize adverse effects of SDB on individuals lives.

Evaluation of the lingual frenulum in newborns using two protocols and its association with breastfeeding.

OBJECTIVE: To characterize the lingual frenulum of full-term newborns using two different protocols and to assess the association of the lingual frenulum with breastfeeding. METHODS: This non-probabilistic sample consisted of 449 mother/baby binomials. For the anatomo-functional evaluation of the frenulum, the Neonatal Tongue Screening Test and the Bristol Tongue Assessment Tool were used for the evaluation of the lingual frenulum. Breastfeeding was evaluated using the protocol proposed by UNICEF. Scores were created (good, fair, poor) to evaluate every aspect of the breastfeeding to be observed. The results were analyzed through descriptive and inferential statistics and association tests (Pearson's chi-squared and Fisher's exact test). RESULTS: The study showed that 14 babies had a lingual frenulum alteration, of whom three had difficulties during suction, requiring frenotomy in the first week of life, whereas 11 had no difficulties during breastfeeding. Regarding the breastfeeding evaluation, 410mother/baby binomials had good, 36 regular, and three had bad scores. There was a statistically significant association between the tongue-tie test protocol and breastfeeding (p=0.028) and between the Bristol Tongue Assessment Tool protocol and breastfeeding (p=0.028). CONCLUSION: Alterations in the lingual frenulum are associated with interferences in the quality of breastfeeding and thus, evaluation of the lingual frenulum in newborns is important.

Dental development in children born to Zikv-infected mothers: a case-based study.

BACKGROUND: In Brazil, in October 2015, an outbreak of Zika virus (ZIKV) infection and an increase in newborns with microcephaly suggested a relationship between maternal infection and microcephaly in children. OBJECTIVE: First, to assess the presence of dental bud sin 13 infants with a confirmed diagnosis of congenital ZIKV syndrome, born to mothers infected with the virus during pregnancy; second, to evaluate the dental development of these children at a 36-month follow-up. DESIGN: Case-based longitudinal study. RESULTS: Dental radiographs in the first semester showed that all children had dental buds. Along with the study, the individuals presented with various dental disturbances. At the end of the evaluation period, some children still had incomplete deciduous dentition. CONCLUSION: The abnormal chronology of dental eruption and dental development disturbances in children with microcephaly infected with ZIKV born to infected mothers indicate a possible role of the virus in odontogenesis.

Prevalence of TMD and level of chronic pain in a group of Brazilian adolescents.

AIMS: To determine the prevalence of temporomandibular disorders and associated factors in an adolescent sample from Recife, Brazil. MATERIALS AND METHODS: A cross-sectional study was conducted with 1342 adolescents aged 10-17 years. The Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) was used by calibrated examiners to evaluate the presence and levels of chronic pain. To evaluate the socioeconomic conditions, the subjects answered the Brazilian Economic Classification Criteria (CCEB) questionnaire. Data were analyzed by means of binary logistic regression in SPSS. RESULTS: The results showed that 33.2% of the subjects had TMD irrespective of age (p = 0.153) or economic class (p = 0.653). Statistically significant associations were found between TMD and female gender (p = 0.017), headache/migraine in the past six months (p<0.001), chronic pain (p<0.001) and chronic pain level (p<0.001). In the final model, logistic regression showed that the level of chronic pain and the headache/migraine in the past six months were related to the presence of TMD. CONCLUSIONS: The prevalence of TMD was considered high (33.2%) and adolescents with chronic pain and headache in the past six months were more likely to have TMD. CLINICAL RELEVANCE: The data contribute to the understanding of TMD among adolescents and to the development of preventive measures and polices to identify the dysfunction promptly.

Remineralizing Potential of Nano-Silver-Fluoride for Tooth Enamel: An Optical Coherence Tomography Analysis

Objective: To evaluate the use of nanosilver fluoride in the enamel remineralization process through optical coherence tomography. Material and Methods: All samples were submitted to demineralization process by pH cycling during 14 days and randomly distributed into three groups (n = 11): Nanosilver Fluoride (NSF), Sodium fluoride (NaF), and negative control. Optical coherence tomography images were acquired at three different moments: initial stage (T0), post caries formation (T1), and post pH cycling (T2). The integrity of the enamel surface and the measurement of the volume loss for the tissue after pH cycling in comparison to initial images were obtained from optical coherence tomography images. Results: After analyzing the exponential decay of A-scans from each group, it was possible to identify differences in light propagation among samples. In T1 it is not possible to visualize the dentin-enamel junction, probably due to the higher back scattering of the demineralized enamel, which does not allow light to reach the dentin. The decay curves obtained from NaF and nanosilver fluoride groups showed similar behavior, while the negative group showed lower extinction coefficient. Conclusion: Nanosilver fluoride showed the best effect against caries compared to conventional fluoride treatments.