Antioxidant Thermogelling Formulation for Burn Wound Healing.

Burns are the most common and devastating form of wounds and are usually accompanied by abnormal inflammation, inadequate extracellular matrix production, reduced angiogenesis, and lack of growth factor stimulation, which can significantly delay wound healing and lead to complications. Pearl powder, a traditional Chinese medicine, has been used to treat wound healing. In the present study, we found that supercritical CO2 extracted pearl peptides in the size range of ">10kd" have great potential to promote wound healing at the cellular level. Antioxidant hydrogels were designed using selenium-containing block-functionalized PEG/PPG polymers. Pearl peptide components were combined into polymeric materials to develop new promising wound dressings. It was shown that pearl peptide hydrogels increased skin fibroblast viability, enhanced cellular resistance to oxidative stress, enhanced tissue remodeling, promoted angiogenesis, and exhibited enhanced promotion in wound healing.

Cationic Lignin-Based Hyperbranched Polymers to Circumvent Drug Resistance in Pseudomonas Keratitis.

The rise of antimicrobial-resistant bacteria strains has been a global public health concern due to their ability to cause increased patient morbidity and a greater burden on the healthcare system. As one of the potential solutions to overcome such bacterial infections, hyperbranched copolymers with cationic charges were developed. These copolymers were assessed for their antimicrobial efficacy and their bactericidal mechanisms. They were found to be potent against mobile colistin-resistant 1 strains, which was significant as colistin is known to be the last-resort antibiotic against Gram-negative bacteria. Furthermore, there was no sign of mutational resistance developed by E. ColiATCC 25922 and MCR 1+E. Coli against the copolymer even up to 20 passages. The ability to evade inducing resistance would provide invaluable insights for future antibiotic development. Our studies suggest that the bactericidal efficacy comes from the ability to target the outer membrane efficaciously. In vivo study using a Pseudomonas keratitis model showed that the copolymer was compatible with the eye and further supported that the copolymer treatment was effective for complete bacteria elimination.

Efficacy of Water-Soluble Pearl Powder Components Extracted by a CO2 Supercritical Extraction System in Promoting Wound Healing.

Pearl powder is a biologically active substance that is widely used in traditional medicine, skin repair and maintenance. The traditional industrial extraction processes of pearl powder are mainly based on water, acid or enzyme extraction methods, all of which have their own drawbacks. In this study, we propose a new extraction process for these active ingredients, specifically, water-soluble components of pearl powder extracted by a CO2 supercritical extraction system (SFE), followed by the extraction efficiency evaluation. A wound-healing activity was evaluated in vitro and in vivo. This demonstrated that the supercritical extraction technique showed high efficiency as measured by the total protein percentage. The extracts exhibited cell proliferation and migration-promoting activity, in addition to improving collagen formation and healing efficiency in vivo. In brief, this study proposes a novel extraction process for pearl powder, and the extracts were also explored for wound-healing bioactivity, demonstrating the potential in wound healing.

Pearl Powder-An Emerging Material for Biomedical Applications: A Review.

Pearl powder is a well-known traditional Chinese medicine for a variety of indications from beauty care to healthcare. While used for over a thousand years, there has yet to be an in-depth understanding and review in this area. The use of pearl powder is particularly growing in the biomedical area with various benefits reported due to the active ingredients within the pearl matrix itself. In this review, we focus on the emerging biomedical applications of pearl powder, touching on applications of pearl powder in wound healing, bone repairing, treatment of skin conditions, and other health indications.

Extraction, Purification, Bioactivities and Application of Matrix Proteins From Pearl Powder and Nacre Powder: A Review.

Natural pearls are formed when sand or parasites (irritants) accidentally enter into the oyster body and form pearls under the cover of the nacre layer. Pearl powder is a powdery substance by grinding pearls into small grains, however, the nacre powder is the inner layer of outer corner layer and middle prism layer. Pearl medicine in China has a history of more than 2,000 years, pearl has the effects of calming the mind, clearing the eyes, detoxifying the muscle and so on. In this paper, the researches on the extraction of pearl powder and nacre powder, the isolation and purification of matrix protein and the various biological activities (osteogenic activity, antioxidant, anti-inflammatory, anti-apoptotic, promoting the migration of fibroblasts, and so on) are reviewed in detail. To provide readers with a faster understanding, the method of extraction and purification and the application of nacre powder and pearl powder are clearly presented in the form of figures and tables. In line with the concept of waste or by-product, there are more reports of nacre extract than pearl extract, due to the expensive and limited in origin of pearls. Mainly on the direct use of nacre powder and pearl powder or on the use of extracts (mainly water soluble proteins) through experiments in vivo or in vitro, and shows whether it is effective through the results of various indexes. There is no further study on substances other than extracts, and the structural analysis of extracts needs further exploration.

Novel bio-inspired three-dimensional nanocomposites based on montmorillonite and chitosan.

In this study, inspired by nacre-like structural natural shells, novel three-dimensional (3D) nanocomposites based on natural nanoplatelets of montmorillonite (MMT) and polysaccharide of chitosan (CS) were prepared with solution intercalation and self-assembly process. The CS-intercalated-MMT nanoplatelets units acted as "bricks" and CS molecules acted as "mortar", arranging in fairly well-ordered layered structure. With addition of glutaraldehyde (GA) and Pd2+ cations, synergistic toughening and strengthening effects of covalent and ionic bonds could be achieved. The best mechanical properties of the prepared 3D nanocomposites were observed as 5.6 KJ/m2 (impact strength), 3.3 GPa (flexural modulus), and 65.8 MPa (flexural strength), respectively, which showed higher toughness but lower flexural properties than natural pearl mussel shells. Nevertheless, both the impact and flexural properties of the prepared 3D nanocomposite were much higher than the other natural shell, i.e. green grab shell. Besides conventional methods characterizations, the nacre-like structure of the artificial 3D nanocomposite was further evidenced with positron annihilation lifetime spectroscopy characterizations. This work might facilitate a versatile platform for developing green 3D bionanocomposites with fairly good mechanical properties.