RESUMO
We presented the structure of the sulfated polysaccharide moiety and anticancer activity in vitro of the Ð-deacylated lipopolysaccharide (DPS) isolated from the marine bacterium Poseidonocella pacifica KMM 9010T. The structure of O-polysaccharide was investigated by chemical methods along with 1H and 13C NMR spectroscopy. The O-polysaccharide was built up of sulfated disaccharide repeating units consisted of d-rhamnose (d-RhaÑ) and 3-deoxy-d-manno-oct-2-ulosonic acid (Kdop): â7)-ß-KdoÑ4Ac5S-(2â3)-ß-d-RhaÑ2S-(1â. We demonstrated that the DPS from P. pacifica KMM 9010T non-toxic for normal mouse epidermal cells (JB6 Cl41 cell line) and inhibited colony formation of human colorectal carcinoma HT-29, breast adenocarcinoma MCF-7 and melanoma SK-MEL-5 cells in a dose-dependent manner.