RESUMO
Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, a new kinetic method for assaying the halogenating activity of MPO is described. The method is based on measuring the rate of iodide-catalyzed oxidation of celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). The latter is produced in a reaction of taurine with HOCl (HOBr). CB is not a substrate for the peroxidase activity of MPO and does not react with hydrogen peroxide and superoxide anion radical. Taurine N-chloramine (bromamine) reacts with CB in molar ratio of 1:2. Using the new method, we studied the dependence of MPO activity on concentration of substrates and inhibitors. The specificity of MPO inhibition by non-proteolyzed ceruloplasmin is characterized. The inhibition of taurine N-chloramine production by neutrophils and HL-60 cells in the presence of MPO-affecting substances is demonstrated. The new method allows determining the kinetic parameters of MPO halogenating activity and studying its inhibition by various substances, as well as screening for potential inhibitors of the enzyme.
Assuntos
Corantes/química , Ensaios Enzimáticos/métodos , Halogenação , Oxazinas/química , Peroxidase/metabolismo , Taurina/análogos & derivados , Brometos/química , Ceruloplasmina/metabolismo , Inibidores Enzimáticos/metabolismo , Células HL-60 , Humanos , Cinética , Neutrófilos/enzimologia , Peroxidase/análise , Peroxidase/antagonistas & inibidores , Taurina/químicaRESUMO
The technology of obtaining of specific immunoglobulin for serotherapy of neuroinfection caused by virus herpes simplex 1 type was developed. The patients presented with the following diseases: arachnoencephalitis, encephalopolyradiculoneuritis, encephalomyelitis, encephalitis, arachnoiditis, polyneuropathy, encephalomyelopolyradiculoneuritis, meningoencephalitis. The study showed good tolerance and safety of the medicine, no adverse effects registered during the study. The assessed median score of the efficacy was 2.8 from 3. The obtained results suggest using the liquid form preparation for intramuscular injection "Immunoglobulin for treatment of neuroinfection caused by virus herpes simplex type 1". The Close corporation "Biofarma" located in Kyiv produces this medicine.
Assuntos
Viroses do Sistema Nervoso Central/tratamento farmacológico , Herpes Simples/tratamento farmacológico , Herpesvirus Humano 1/efeitos dos fármacos , Imunoglobulina G/uso terapêutico , Viroses do Sistema Nervoso Central/imunologia , Viroses do Sistema Nervoso Central/virologia , Esquema de Medicação , Herpes Simples/imunologia , Herpes Simples/virologia , Humanos , Imunoglobulina G/administração & dosagem , Imunoglobulina G/efeitos adversos , Injeções Intramusculares , Resultado do TratamentoRESUMO
The influence of an alternating (50 Hz) electric field (5--110 V/cm) on the state of human buccal epithelium cells was studied by the methods of intracellular microelectrophoresis, heterochromatin staining with orcein, and indigo carmine staining for viability and membrane integrity evaluations. Electric field exposure induced an increase in electrophoretic mobility of cell nuclei, decreased numbers of heterochromatin granules near the inner membrane of cell nucleus, and induced cell membrane damage; but cell viability was conserved. Nuclear and cell membrane properties varied with electric field strength and age of the donors. The data obtained are interpreted as evidence of electric field induced activation of the functional state of nuclei.
Assuntos
Campos Eletromagnéticos/efeitos adversos , Mucosa Bucal/metabolismo , Adulto , Idoso , Envelhecimento/metabolismo , Membrana Celular/metabolismo , Núcleo Celular/metabolismo , Bochecha , Células Epiteliais/metabolismo , Feminino , Heterocromatina/metabolismo , Humanos , Técnicas In Vitro , Cinética , Pessoa de Meia-Idade , Mucosa Bucal/citologiaRESUMO
Investigations were conducted designed to study effects of the complex of vitamins with minerals "Multi-Tabs" (Denmark) as a preventive measure devised to combat influenza during the 1997 influenza epidemic in Ukraine. The article contains epidemiological characterization of the above epidemic. Used in 160 adults and 55 children "Multi-Tabs" has been shown to be an effective means of the influenza and ARD prophylaxis in an epidemic setting both in adults and children. The above drug is also endowed with a marked hemostimulating activity, which fact was manifested by improvement in parameters characterizing red blood.
Assuntos
Antivirais/uso terapêutico , Influenza Humana/prevenção & controle , Minerais/uso terapêutico , Vitaminas/uso terapêutico , Adulto , Criança , Combinação de Medicamentos , Avaliação de Medicamentos , Feminino , Hematopoese/efeitos dos fármacos , Humanos , Influenza Humana/sangue , MasculinoRESUMO
An original method for the determination of cell membrane permeability has been developed. Human buccal epithelium cells were treated with Indigo Carmine and the percentage of stained cells was determined. Employing this method, the age-related differences in properties of plasma membrane in cells of human buccal epithelium were demonstrated. In the group of aged donors the average level of cell stainability was higher than in the group of younger donors. These results demonstrate a decrease in outer cell membrane integrity with age.