RESUMO
Methylmercury is a highly toxic, organic derivative found in mercury-polluted wetlands and coastal sediments worldwide. Though commonly present at low concentrations in the substrate, methylmercury can biomagnify to concentrations that poison predatory animals and humans. In the interest of developing an in situ detoxification strategy, a model plant system was transformed with bacterial genes (merA for mercuric reductase and merB for organomercurial lyase) for an organic mercury detoxification pathway. Arabidopsis thaliana plants expressing both genes grow on 50-fold higher methylmercury concentrations than wild-type plants and up to 10-fold higher concentrations than plants that express merB alone. An in vivo assay demonstrated that both transgenes are required for plants to detoxify organic mercury by converting it to volatile and much less toxic elemental mercury.
Assuntos
Arabidopsis/genética , Engenharia Genética/métodos , Substâncias Perigosas/metabolismo , Compostos Organomercúricos/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Poluentes Atmosféricos/metabolismo , Arabidopsis/enzimologia , Biodegradação Ambiental , Resistência a Medicamentos , Ecologia , Gases , Liases/genética , Liases/metabolismo , Mercúrio/metabolismo , Compostos de Metilmercúrio/metabolismo , Compostos de Metilmercúrio/farmacologia , Modelos Biológicos , Compostos Organomercúricos/farmacologia , Oxirredutases/genética , Oxirredutases/metabolismo , Fenótipo , Compostos de Fenilmercúrio/metabolismo , Compostos de Fenilmercúrio/farmacologia , Poluentes do Solo/metabolismo , Poluentes Químicos da Água/metabolismoRESUMO
Methylmercury is an environmental toxicant that biomagnifies and causes severe neurological degeneration in animals. It is produced by bacteria in soils and sediments that have been contaminated with mercury. To explore the potential of plants to extract and detoxify this chemical, we engineered a model plant, Arabidopsis thaliana, to express a modified bacterial gene, merBpe, encoding organomercurial lyase (MerB) under control of a plant promoter. MerB catalyzes the protonolysis of the carbon---mercury bond, removing the organic ligand and releasing Hg(II), a less mobile mercury species. Transgenic plants expressing merBpe grew vigorously on a wide range of concentrations of monomethylmercuric chloride and phenylmercuric acetate. Plants lacking the merBpe gene were severely inhibited or died at the same organomercurial concentrations. Six independently isolated transgenic lines produced merBpe mRNA and MerB protein at levels that varied over a 10- to 15-fold range, and even the lowest levels of merBpe expression conferred resistance to organomercurials. Our work suggests that native macrophytes (e.g., trees, shrubs, grasses) engineered to express merBpe may be used to degrade methylmercury at polluted sites and sequester Hg(II) for later removal.
Assuntos
Poluentes Atmosféricos/toxicidade , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Proteínas de Bactérias/genética , Liases , Compostos de Metilmercúrio/toxicidade , Biodegradação Ambiental , Regulação da Expressão Gênica de Plantas , Genes Bacterianos , Plantas Geneticamente ModificadasRESUMO
We examined the ability of yellow poplar (Liriodendron tulipifera) tissue cultures and plantlets to express modified mercuric reductase (merA) gene constructs. Mercury-resistant bacteria express merA to convert highly toxic, ionic mercury, Hg(II), to much less toxic, elemental mercury, Hg(O). Expression of merA in transgenic plants might provide an ecologically compatible approach for the remediation of mercury pollution. Because the alteration of the bacterial merA gene sequence is necessary for high-level expression in Arabidopsis thaliana, yellow poplar proembryogenic masses (PEMs) were transformed with three modified merA constructs via microprojectile bombardment. Each construct was synthesized to have altered flanking regions with increasing amounts of modified coding sequence. All merA constructs conferred resistance to toxic, ionic mercury in independently transformed PEM colonies. Stability of merA transgene expression increased in parallel with the extent of gene coding sequence modification. Regenerated plantlets containing the most modified merA gene (merA18) germinated and grew vigorously in media containing normally toxic levels of ionic mercury. The merA18 plantlets released elemental mercury at approximately 10 times the rate of untransformed plantlets. These results indicate that plants expressing modified merA constructs may provide a means for the phytoremediation of mercury pollution.
Assuntos
Poluentes Ambientais/metabolismo , Mercúrio/metabolismo , Plantas Geneticamente Modificadas , Árvores/genética , Biodegradação Ambiental , Regulação Enzimológica da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Oxirredutases/genética , Árvores/enzimologia , Árvores/metabolismoRESUMO
With global heavy metal contamination increasing, plants that can process heavy metals might provide efficient and ecologically sound approaches to sequestration and removal. Mercuric ion reductase, MerA, converts toxic Hg2+ to the less toxic, relatively inert metallic mercury (Hg0) The bacterial merA sequence is rich in CpG dinucleotides and has a highly skewed codon usage, both of which are particularly unfavorable to efficient expression in plants. We constructed a mutagenized merA sequence, merApe9, modifying the flanking region and 9% of the coding region and placing this sequence under control of plant regulatory elements. Transgenic Arabidopsis thaliana seeds expressing merApe9 germinated, and these seedlings grew, flowered, and set seed on medium containing HgCl2 concentrations of 25-100 microM (5-20 ppm), levels toxic to several controls. Transgenic merApe9 seedlings evolved considerable amounts of Hg0 relative to control plants. The rate of mercury evolution and the level of resistance were proportional to the steady-state mRNA level, confirming that resistance was due to expression of the MerApe9 enzyme. Plants and bacteria expressing merApe9 were also resistant to toxic levels of Au3+. These and other data suggest that there are potentially viable molecular genetic approaches to the phytoremediation of metal ion pollution.
Assuntos
Arabidopsis/genética , Arabidopsis/metabolismo , Poluentes Ambientais/metabolismo , Poluentes Ambientais/toxicidade , Mercúrio/metabolismo , Mercúrio/toxicidade , Oxirredutases/genética , Oxirredutases/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Mapeamento Cromossômico , DNA Recombinante/genética , Resistência a Medicamentos/genética , Escherichia coli/enzimologia , Escherichia coli/genética , Genes Bacterianos , Dados de Sequência Molecular , Oxirredução , Plantas Geneticamente Modificadas , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
The autumn of 1994 has arrived. At this same time last year, most political pundits and policy wonks proclaimed that the Congress would surely take action to overhaul the American health care system before this year's November elections. But as 1994 draws to a close, it has become apparent that Washington is unlikely to produce comprehensive reform legislation at any time in the near future. However, while our political system has failed to act in any meaningful way, its inability to chart a course for change has not quelled the movement to reshape our nation's health care system. Despite Congressional inertia, substantial health care reform is taking place both in Michigan and throughout the nation. These reforms are characterized by a central theme: rapid movement away from our historic focus on illness, and toward a more rational focus on health. Our commitment to healthy communities stands as a firm testament to our mission of service and our role as the central core of the health care delivery system. This article will address the various components of that paradigm shift, and highlight some of the outstanding efforts of Michigan hospitals as they partner with their communities to reshape the delivery of health care services.