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1.
J Invertebr Pathol ; 166: 107224, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31362005

RESUMO

When Colorado potato beetle larvae ingested potato plants treated with the plant defense inducer compound hexanoic acid, midgut chymotrypsin enzyme activity increased, and the corresponding chymotrypsin genes were differentially expressed, evidence of the larval digestive proteolytic system's plasticity. We previously reported increased susceptibility to Cry3Aa toxin in larvae fed hexanoic acid treated plants. Here we show that the most expressed chymotrypsin gene in larvae fed hexanoic acid treated plants, CTR6, was dramatically downregulated in Cry3Aa intoxicated larvae. lde-miR-965-5p and lde-miR-9a-5p microRNAs, predicted to target CTR6, might be involved in regulating the response to hexanoic acid but not to Cry3Aa toxin.


Assuntos
Proteínas de Bactérias/farmacologia , Caproatos/farmacologia , Quimotripsina/biossíntese , Besouros/enzimologia , Endotoxinas/farmacologia , Genes de Insetos , Proteínas Hemolisinas/farmacologia , Animais , Toxinas de Bacillus thuringiensis , Quimotripsina/genética , Besouros/efeitos dos fármacos , Besouros/genética , Sistema Digestório/enzimologia , Regulação da Expressão Gênica/efeitos dos fármacos , Genes de Insetos/efeitos dos fármacos , Genes de Insetos/fisiologia , Larva , Solanum tuberosum/efeitos dos fármacos , Solanum tuberosum/parasitologia
2.
J Plant Physiol ; 215: 59-64, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28578135

RESUMO

Insect-plant interactions are governed by a complex equilibrium between the mechanisms through which plant recognize insect attack and orchestrate downstream signaling events that trigger plant defense responses, and the mechanisms by which insects overcome plant defenses. Due to this tight and dynamic interplay, insight into the nature of the plant defense response can be gained by analyzing changes in the insect herbivores digestive system upon plant feeding. In this work we have identified a Solanum melongena miraculin-like protease inhibitor in the midgut juice of Colorado potato larvae feeding on eggplant plants treated with the natural inducer of plant defenses hexanoic acid. We analyzed the corresponding gene expression by qRT-PCR and our results showed that this eggplant miraculin-like gene enhanced induction contributes to the hexanoic acid priming effect in this Solanaceae species. Moreover, our data evidencing that OPDA might be involved in this gene regulation highlights its potential as biomarker in eggplant plant responses to stress mediated this oxylipin signaling pathway.


Assuntos
Caproatos/farmacologia , Besouros/patogenicidade , Oxilipinas/metabolismo , Inibidores de Proteases/farmacologia , Solanum melongena/metabolismo , Solanum melongena/parasitologia , Animais , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/genética , Solanum melongena/efeitos dos fármacos
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