The moderating role of age and seniority on nurses' emotional dissonance and perceived health.

This study aims to investigate the weight of surface acting (a condition in which subjects must display an emotional state that does not correspond to their real feelings) in the relationship between the emotional load of nursing work and the perception of health, and to evaluate the moderating effect of age and length of service. A moderated mediation analysis was conducted on a sample of 359 Italian nurses. The results confirmed the mediation role of surface acting and showed that both age and seniority have moderating effects so that in conditions of high emotional load, older and more experienced nurses show higher levels of surface acting, and in conditions of high surface acting, younger and less experienced nurses show lower levels of perceived health. In conclusion, surface acting seems a stressor for younger nurses, not yet used to the weight of faking emotions, while the perceived emotional load seems a stressor for older and more experienced nurses. Each result highlights the importance of providing emotional regulation skills training and support to reduce the psychological impact of emotional demands on nurses.

Meaningful work, pleasure in working, and the moderating effects of deep acting and COVID-19 on nurses' work.

AIMS: This study aims to verify the association between nurses' perception of the meaningfulness of their work and their pleasure in working, and whether this relationship may change based on the level of deep acting performed to cope with emotional regulation demands and the influence of the COVID-19 pandemic on the healthcare work. METHODS: Nurses from both private and public Italian institutions (N = 239) completed an online questionnaire between June 2021 and January 2022. A moderated moderation model was tested through SPSS Process macro. The design is cross-sectional. RESULTS: The results show that the perception of meaningfulness of work is positively associated with pleasure in working, especially in conditions of high deep acting. This relationship is further moderated by the COVID-19 influence so that the association between meaningful work and pleasure in working is stronger in conditions of high COVID-19 influence and at higher levels of deep acting performed. CONCLUSION: Perceiving one's work as meaningful can be a job resource that protects nurses from the negative effects of emotional regulation demands and even from the stress of dealing with COVID-19. IMPACT: The study addresses the problem of nurses' emotional regulation demands at work and evaluates the protective role of meaningful work. The findings could be useful for planning prevention interventions (through training in adaptive emotional regulation strategies) or protection interventions (through the promotion of effective coping strategies and the stimulation of one's work engagement).

Examination of the relationship between emergency department presentations and population mortality: a multicenter analysis of emergency department presentations during the COVID-19 pandemic.

BACKGROUND: In the spring of 2020, Italy experienced a significant reduction in the number of emergency department (ED) presentations during the first wave of the COVID-19 pandemic. If ED access has an impact on patients' prognosis, such a reduction in ED presentations would be expected to correlate with a parallel increase in the mortality rate of the corresponding population. The aim of the present study was to evaluate the impact of reduced ED presentations on the all-cause mortality of the general population. METHODS: Absolute and relative variation in ED accesses from March 1 to April 30 of both 2019 and 2020 in three hub hospitals in areas with different COVID-19 prevalence and age-standardized mortality data from January 1 to June 30 in 2019 and 2020 of the same areas were evaluated. RESULTS: During March and April 2020, ED consults were decreased of approximately 50% in all three hospitals, as compared with the same months in 2019. There was a marked increase in cumulative mortality in Milan (high SARS-CoV2 infection spread zone) compared with the same period in 2019. In the other two municipalities (Ferrara and Perugia), which had intermediate and low levels of infection spread, the mortality in 2020 was not substantially changed from that of 2019. CONCLUSIONS: Taking into account the increase in mortality due to SARS-CoV-2, reductions in ED access did not seem to affect death rates. If this finding will be confirmed, ED organization and access would need to be reconsidered.

Detection of resistance protein A (MxA) in paper-based immunoassays with surface enhanced Raman spectroscopy with AuAg nanoshells.

Myxovirus protein A (MxA) is a biomarker that can be used to distinguish between viral and bacterial infections. While MxA lateral flow assays (LFAs) have been successfully used for viral vs. bacterial differential diagnosis for children, the clinically relevant level of MxA for adults has been reported to be 100 times lower, which is too low for traditional LFAs. We present results applying the use of surface enhanced Raman spectroscopy (SERS) to detect MxA. AuAg nanoshells (AuAg NSs) were used to enhance the Raman signal of mercaptobenzoic acid (4-MBA), enabling readout by SERS. The AuAg NSs were conjugated to antibodies for the biomarker of interest, resulting in a "nanotag", that could be used in a dipstick immunoassay for detection. We first optimized the nanotag parameters using anti-human IgG/human IgG as a model antibody/antigen system, and then demonstrated detection of MxA using anti-MxA antibodies. We show that SERS readout of immunoassays for MxA can quantify MxA levels at clinically relevant levels for adult viral infection.

Formation of the Protein Corona: The Interface between Nanoparticles and the Immune System.

The interaction of inorganic nanoparticles and many biological fluids often withstands the formation of a Protein Corona enveloping the nanoparticle. This Protein Corona provides the biological identity to the nanoparticle that the immune system will detect. The formation of this Protein Corona depends not only on the composition of the nanoparticle, its size, shape, surface state and exposure time, but also on the type of media, nanoparticle to protein ratio and the presence of ions and other molecular species that interfere in the interaction between proteins and nanoparticles. This has important implications on immune safety, biocompatibility and the use of nanoparticles in medicine.

Enhanced electrogenerated chemiluminescence in thermoresponsive microgels.

The electrochemistry, photoluminescence and electrogenerated chemiluminescence of thermoresponsive redox microgels were investigated. For the first time, reversible ECL enhancement is demonstrated in stimuli-responsive 100-nm microgel particles. Such an unexpected amplification reached 2 orders of magnitude, and it is intrinsically correlated with the collapse of the microgel particles. The swell-collapse transition decreases the average distance between adjacent redox sites and favors the electron-transfer processes in the microgels resulting in the enhanced ECL emission.