[Effect of lysozyme on the functional and metabolic activity of polymorphonuclear leukocytes in the acute blood loss]. / Vliianie lizotsima na funktsional'no-metabolicheskuiu aktivnost' polimorfno-iadernykh leikotsitov v usloviiakh ostroi krovopoteri.

Acute hemorrhage leads to a decrease in the phagocyte number and index, the NBT test parameters, and the NADPH oxidase activity and to an increase in the Ca2+ ATPase activity in polymorphonuclear leukocytes on the peripheral blood. Lysozyme produces effective correction of the above parameters of the functional-metabolic activity of these cells. The drug effect is mediated by cytokines of splenocytes sticking to the glass.

[Immuno-metabolic effects of lysozyme in acute blood loss]. / Immunometabolicheskie éffekty, vyzyvaemye lizotsimom pri ostroi krovopotere.

Lysozyme injections (1 or 5 mg/kg) produced marked immunomodulating, antioxidant and hepatoprotective effects in Wistar rats which had lost much blood (3% body mass). Lysozyme action is mediated by heavy erythrocytes and glass-adherent splenocytic cytokines the production of which is provoked by these erythrocytes.

[Immunomodulating effect of pyridoxine, folate, and cobalamin in various forms of anemia]. / Immunomoduliruiushchee deistvie piridoksina, folata i kobalamina pri razlichnykh formakh anemii.

Hemorrhage and toxic lesions of hemopoietic tissues inhibit the development of various types of immune response and induce immunosuppressive properties in light erythrocytes, while not influencing the immunological activity of heavy erythrocytes. The immunomodulant action of pyridoxine, folate and cobalamin in various cases of anemia was observed for the joint (but not for separate) administration of two vitamin preparations; the most pronounced increase in the immune reactivity taking place upon the joint administration of cobalamin and folate.

[Immunomodulating activity of erythrocytes and their stroma incubated with menadion and heteropolysaccharides Polygonaceae in case of acute hemorrhage]. / Immunomoduliruiushchee deistvie éritrotsitov i ikh stromy, inkubirovannykh s menadionom i geteropolisakharidami Polygonaceae, pri ostroi krovopotere.

Effect of animal erythrocytes incubated with glycosaminoglycanes and menadion on the immune response of mice and rats with acute hemorrhage was investigated. The results of the study proves the interest in elaboration of the methods to produce erythrocytes stroma incubated extracorporately with menadion and glucosaminoglycanes. This preparation may be useful for immunomodulating effect in the case of acute hemorrhage and also in other stress situations, connected with changes of erythrocytes properties and activity.

[Correlation of immunomodulating effects induced by lysozyme and naphthoquinones in acute blood loss]. / Vzaimosviaz' immunomoduliruiushchikh éffektov, vyzyvaemykh lizotsimom i naftokhinonami, pri ostroi krovopotere.

The role of erythrocytes in realization of interrelation of the immunomodulating effects of lysozyme and naphthoquinones in normal and under conditions of acute hemorrhage was studied. Interaction of lysozyme and menadione at the level of intact erythrocytes and their stroma resulted in formation of highly efficient immunomodulating factors. The effect of such factors under conditions of acute hemorrhage was mediated by cytokines of the spleen macrophagael cells.

[Naphthoquinones as immunomodulators during intensive exertion]. / Naftokhinony kak immunomoduliatory pri intensivnykh fizicheskikh nagruzkakh.

Naphthoquinones such as phylloquinone, menadione and vicasol normalized or increased the immune response in rats after intensive physical load. The most marked immunomodulating effect was induced by phylloquinone. The naphthoquinones induced the development of immunostimulating properties in heavy red blood cells by the direct action on the cell membranes or indirectly through proteolytic enzymes secreted by hepatocytes. The proteolytic enzymes secreted to the vascular channel in physical load increased the susceptibility of heavy red blood cells to phylloquinone. Glycosaminoglycans accumulating in the vascular channel in physical load increased the resistance of heavy red blood cells to phylloquinone. Heavy red blood cells of physically loaded animals treated in succession with hyaluronidase and phylloquinone and red blood cells of intact rats after the incubation in the presence of trypsin and phylloquinone proved to be effective immunomodulators in physical load.