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Arch Microbiol ; 195(6): 431-8, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23624722

RESUMO

Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane. We have cloned and sequenced the genes coding for the components of the iron ABC-type acquisition system of G. diazotrophicus. Sequence analysis revealed three ORFs, (feuA, feuB, and feuC) organized as an operon and encoding polypeptides of 346 (38 kDa), 342 (34.2 kDa), and 240 (26 kDa) amino acids, respectively. The deduced translation products of the feu operon showed similarity with a periplasmic solute-binding protein (FeuA), permease (FeuB), and ATPase (FeuC) involved in Fe transport. The role of FeuB in the survival of G. diazotrophicus under iron depletion was evaluated by comparing the ability of wild-type and FeuB-Km(R) -mutant strains in a medium without iron supplementation and in a medium containing 2, 2'-dipyridyl (DP). Growth of the mutant was affected in the medium containing DP. The operon was expressed at higher levels in cells depleted for iron than in those that contained the metal. A decrease in nitrogenase activity was observed with the FeuB-Km(R) -mutant strain that with the wild-type under iron deficiency conditions, suggesting that the Feu operon play role in Fe nutrition of G. diazotrophicus.


Assuntos
Proteínas de Bactérias/genética , Gluconacetobacter/genética , Ferro/metabolismo , Óperon , Transportadores de Cassetes de Ligação de ATP/genética , Adenosina Trifosfatases/genética , Transporte Biológico , Gluconacetobacter/metabolismo , Proteínas de Membrana Transportadoras/genética , Fixação de Nitrogênio , Proteínas Periplásmicas de Ligação/genética , Saccharum/microbiologia
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