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1.
Diagn Microbiol Infect Dis ; 109(3): 116284, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38604077

RESUMO

BACKGROUND: Searching for Rheumatoid Factors (RF) in patients with coronavirus disease (COVID-19) has rarely been described. OBJECTIVES: To investigate the association between RF isotypes (IgM, IgA, and IgG) and different clinical presentations of COVID-19 in a series of Tunisian patients. STUDY DESIGN: Eighty-two COVID-19 patients were enrolled in this study. Symptomatic cases were recruited from the Department of COVID-19 and the intensive care unit (ICU) of the University Hospital of Mahdia, Tunisia, from January 2021 to March 2021. Different RF isotypes were assessed using a commercial enzyme-linked immunosorbent assay (ELISA). RESULTS: Forty-one patients (50%) had RF of any isotype. Thirty-two patients (39%) were tested positive for RF-IgM. Symptomatic forms of the disease were associated with RF-IgM positivity (p = 0.005). The mean concentration of RF-IgM was higher in the severe form than in the moderate and asymptomatic forms (p = 0.006). CONCLUSIONS: Our study suggests that the production of RF-IgM isotype is increased in patients with severe COVID-19.


Assuntos
COVID-19 , Imunoglobulina M , Fator Reumatoide , SARS-CoV-2 , Humanos , COVID-19/diagnóstico , COVID-19/imunologia , Fator Reumatoide/sangue , Masculino , Feminino , Pessoa de Meia-Idade , Tunísia/epidemiologia , Imunoglobulina M/sangue , SARS-CoV-2/imunologia , Adulto , Idoso , Imunoglobulina G/sangue , Ensaio de Imunoadsorção Enzimática , Índice de Gravidade de Doença , Imunoglobulina A/sangue
2.
Afr J Reprod Health ; 25(2): 131-137, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37585761

RESUMO

Premature Rupture of Membranes is responsible for most cases of neonatal death. In most of these cases, the causes of PROM have not been established in Tunisia, although several risk factors have been described. Therefore, we set out to determine the presence of an association between genital infections and PROM among Tunisian women. A case-control study was conducted among 251 womens to detect the presence of association between genital tract infection and Premature Rupture of Membranes.Cases had a premature membranes rupture and the controls had intact membranes or suffering from premature membrane rupture during the latent phase of labour. Data were collected from the medical register including socio-demographic characteristics, obstetrics, and medical history. Association between genital infections and premature rupture of membranes was estimated using the Odds Ratio and 95% CI. One risk factor was identified, including age. There is no association between the presence of Group B streptococcus (OR= 1.08; 95% CI 0.50-2.34), presence of Trichomonasvaginalis (OR= 2.45; 95% CI 0.15-39.83) and presence of Candidiasis (OR= 1.11; 95% CI 0.58-2.14) and premature rupture of membranes. Co-infection was not associated with premature rupture of membranes (OR= 0.43; 95% CI 0.45-6.07). There is no association between genital infections and PROM among pregnant Tunisian women.

3.
Hum Immunol ; 77(1): 90-95, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26519864

RESUMO

The purpose of this study was to investigate the HLA-G 3'UTR 14 bp polymorphism and sHLA-G levels in Tunisian patients with BD. The study included 119 patients with BD and 170 healthy blood donors (HD). HLA-G 14 bp polymorphism was genotyped by polymerase chain reaction. Serum levels of soluble HLA-G (sHLA-G) were measured using a commercial ELISA kit. A significant increased frequency of the -14 bp HLA-G allele was detected in patients with BD compared to HD (0.58 vs 0.49, p=0.023), and a significant increased frequency of HLA-G -14/-14 bp was observed in patients with BD compared to HD [0.37 vs 0.22, p=0.007, OR 2.04 (95% CI 1.21-3.42)]. The mean plasmatic concentration of sHLA-G levels were significantly increased in patients with active disease [231.63±286.4 U/mL] compared to those with inactive disease (103.14±77.8 U/mL, p=0.03) and HD (121.41±24.1 U/mL, p=0.04). Furthermore, our results showed that there is no association between HLA-G 14 bp polymorphism and sHLA-G plasma levels.


Assuntos
Síndrome de Behçet/imunologia , Antígenos HLA-G/genética , Regiões 3' não Traduzidas/genética , Adolescente , Adulto , Síndrome de Behçet/genética , Criança , Análise Mutacional de DNA , Progressão da Doença , Feminino , Frequência do Gene , Estudos de Associação Genética , Predisposição Genética para Doença , Genótipo , Antígenos HLA-G/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Mutagênese Insercional/genética , Polimorfismo Genético , Tunísia , Adulto Jovem
4.
J Appl Microbiol ; 119(5): 1443-53, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26248601

RESUMO

AIMS: To ascertain the viral load, the distribution of G and P types of group A rotaviruses (RV-A) in sewage samples and to compare strains in clinical, animal and environmental samples. METHODS AND RESULTS: During our study from April 2007 to April 2010, 518 samples of raw and treated sewage were collected from two biological sewage treatment plants (STPs) located in the Monastir region, Tunisia. RV-A was detected by real-time RT-PCR in 375 (72·4%) sewage samples. According to the quantification results of RV-A, it appears that the viral load in raw and treated sewage of the two STPs was quite similar (P = 0·735). The genotyping of RV-A strains detected in sewage samples showed a great diversity with 10 G types and 8 P types. Most of them were described as common in humans, but we also detected genotypes commonly found in animals. All the genotypes detected in two previous studies performed in our laboratory on clinical and bovine samples were also found in environmental samples. However, some genotypes commonly found in animal were only found in sewage samples. CONCLUSION: The comparison of environmental, clinical and animal data suggests that STPs may convey not only human sewage but also animal wastes, both of them contaminated with numerous RV-A strains which are not efficiently eliminated by the sewage treatment process and may spread to surface waters. SIGNIFICANCE AND IMPACT OF THE STUDY: This work demonstrates the potential release of human and animal RV-A into water sources, representing a public health risk, by inducing gastroenteritis in population, but also by increasing the risk of zoonotic transmission and formation of reassortant viruses which could get a higher infectious potential. Our findings also suggest that monitoring of sewage may provide an additional tool to determine the epidemiology of RV-A circulating in a given community.


Assuntos
Doenças dos Bovinos/virologia , Gastroenterite/veterinária , Gastroenterite/virologia , Rotavirus/isolamento & purificação , Esgotos/virologia , Animais , Bovinos , Genótipo , Humanos , Rotavirus/classificação , Rotavirus/genética , Tunísia
5.
Mater Sci Eng C Mater Biol Appl ; 46: 125-31, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25491968

RESUMO

In this paper, an optical sensor was proposed for sensitive determination of copper (II) ions in aqueous solution. The sensing membrane was prepared by coating ß-ketoimine calix[4]arene derivative as ionophore on a glass plate surface. Thin ß-ketoimine calix[4]arene layer was characterized by contact angle measurements showing a good surface coverage. Besides, a smooth and homogeneous morphology of the calixarene membrane was examined by atomic force microscopy (AFM). The response of the optode is based on the decrease in the absorbance signal at 309 nm upon exposure to buffer solutions containing Cu(2+) ions. At a pH 6.8, the proposed sensor displays a calibration response for copper over a wide concentration range of 1.0×10(-7) M to 1.0×10(-4) M with a detection limit of 2.0×10(-8) M and response time of 7-10 min. This optode exhibits good selectivity toward copper ions in comparison with common ions including Hg(2+) and Ag(+) ions. The copper sensing film can be regenerated using 0.01M HNO3 solution. In addition to its high stability, repeatability and reproducibility, the sensor shows operational life time of 4 weeks.


Assuntos
Calixarenos/química , Cobre/análise , Técnicas Eletroquímicas/instrumentação , Iminas/química , Ionóforos/química , Técnicas Eletroquímicas/métodos , Concentração de Íons de Hidrogênio , Limite de Detecção , Membranas Artificiais , Reprodutibilidade dos Testes , Propriedades de Superfície
6.
J Appl Microbiol ; 116(6): 1387-95, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24905214

RESUMO

AIMS: To investigate the incidence, viral load and genetic diversity of bovine rotaviruses strains in Tunisia. METHODS AND RESULTS: A total of 169 faecal specimens, collected from diarrhoeic calves from several farms located in the central eastern regions of Tunisia, between January 2006 and October 2010, were analysed by semi-nested multiplex RT-PCRs for P and G genotypes identification or were genotyped by DNA sequencing. Positive samples were tested by TaqMan real-time RT-PCR to quantify the viral load. Group A bovine rotaviruses were detected in 15·4% (26/169) of the total studied cases of diarrhoea. Overall, G10 was the predominant G type, detected in 12/26 samples (46·2%) and G6 accounted for 42·3% (11/26) while P[11] was the predominant P type, detected in 12/26 samples (46·2%). Two P[5] genotypes (7·7%) were found in the collection. Dual G or P combination and genotype G8 were not found. The most common VP7/VP4 combinations were G6P[11] (30·8%; n = 8) and G10P[11] (11·5%; n = 3). The combination G10P[14] was seen in one sample, and partial typing was assessed in 53·8% (n = 14) of the cases. The viral load determined by real-time RT-PCR showed an average of 1·68 × 10(9) genome copies/g of faeces. CONCLUSION: Knowledge of P and G types could help us understand the relatedness of animal rotaviruses to viruses causing disease in humans. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first time that the viral load and P types of bovine rotaviruses have been determined in Tunisia, and this study contributes to a better understanding of the epidemiology of such viruses circulating in Tunisia. Nevertheless, continuous surveillance is necessary to detect the emergence of new variants.


Assuntos
Doenças dos Bovinos/epidemiologia , Diarreia/veterinária , Genótipo , Infecções por Rotavirus/veterinária , Rotavirus/genética , Animais , Bovinos , Doenças dos Bovinos/virologia , Diarreia/virologia , Fezes/virologia , Variação Genética , Incidência , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Infecções por Rotavirus/epidemiologia , Estações do Ano , Análise de Sequência de DNA , Tunísia/epidemiologia
7.
Immunol Invest ; 43(5): 463-78, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24661088

RESUMO

The aim of this study was to investigate the subclasses and the immunophenotypic profile of peripheral mononuclear cells in patients with Behçet's disease (BD) and to assess associations between the expression of HLA-B51 antigen and that of other cell markers. Thirty healthy volunteer blood donors and forty patients with BD were enrolled into this study. Phenotyping was performed using two color flow cytometry. HLA-B51 typing was performed using the complement dependent microlymphocytotoxicity assay. Unlike controls, patients with BD presented a modified immunophenotypic profile of lymphocytes. Compared to those in the remission phase, patients with active BD showed an increased mean of MFI ratio of CD56 on CD16+CD56+ cells (32.47 ± 14.26 versus 23.87 ± 10.3; p = 0.032), increased absolute numbers of CD4(-)CD8(bright) and CD4(+)CD8(+) cells (657.1 ± 463.6 cells/µL versus 319.24 ± 116.4 cells/µL; p = 0.017 and 40.77 ± 36.41 cells/µL versus 10.77 ± 9.78 cells/µL; p < 0.0001, respectively) and an elevated mean of MFI ratio of CD19 on B cells (252.3 ± 56.7 versus 205.67 ± 32.3; p = 0.021). However, expression of HLA-B51 was not associated with any specific immunophenotypic profile. In conclusion, abnormal immunophenotypic profile of peripheral lymphocytes was found in patients with BD, especially in active phase, reflecting an immune dysregulation. Moreover, HLA-B51 expression was not found to be related to the expression of other cell markers.


Assuntos
Síndrome de Behçet/imunologia , Síndrome de Behçet/metabolismo , Antígeno HLA-B51/imunologia , Antígeno HLA-B51/metabolismo , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/metabolismo , Fenótipo , Adolescente , Adulto , Antígenos de Superfície/metabolismo , Síndrome de Behçet/diagnóstico , Estudos de Casos e Controles , Criança , Feminino , Humanos , Imunofenotipagem , Masculino , Pessoa de Meia-Idade , Adulto Jovem
8.
J Biotechnol ; 151(1): 114-21, 2011 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-21112357

RESUMO

The electrochemical impedance spectroscopy (EIS) technique has been used as a sensitive method to explore the effect of antibacterial molecules on immobilized bacteria and biofilm formation. In this work, we describe the electrochemical spectroscopy as a powerful method to monitor the effect of chlorhexidine digluconate (CHX-Dg) on polyelectrolyte immobilized Escherichia coli K12 MG1655 and the kinetics of cell adhesion on gold electrodes. The experimental impedance data were modeled with a Zview program to find the best equivalent electrical circuit and analyse its parameter's properties. Polyelectrolyte multilayer formation on the electrode surface and bacteria immobilization greatly increased the electron-transfer resistance (R(et)) and reduced the constant phase element (CPE(dl)). The effect of CHX-Dg was studied in a 0.5 x 10⁻4 mmol l⁻¹ to 0.5 mmol l⁻¹ range. The relation between the evolution of R(et) and CHX-Dg concentration was found to be negatively correlated. When CHX-Dg was added, the electrochemical monitoring of the bacterial kinetic adhesion showed that the electrode's capacity (C(P)) variation remained stable, demonstrating that the addition of CHX-Dg in the broth inhibited bacterial adhesion.


Assuntos
Adesão Celular/efeitos dos fármacos , Células Imobilizadas/efeitos dos fármacos , Clorexidina/análogos & derivados , Técnicas Eletroquímicas/métodos , Escherichia coli/efeitos dos fármacos , Clorexidina/farmacologia , Impedância Elétrica , Eletrodos , Escherichia coli/química , Escherichia coli/citologia , Ouro/química , Microscopia de Força Atômica , Microscopia de Fluorescência
9.
Gastroenterol Clin Biol ; 34(8-9): 483-7, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20655155

RESUMO

AIMS: The purpose of this study was to determine the sensitivity and specificity of IgA anti-actin antibodies (IgA-AAA) for celiac disease (CD), to investigate their usefulness as a marker of compliance in CD patients to the gluten-free diet (GFD), and to assess the relationship between their presence in the sera of CD patients and severity of intestinal mucosal damage. PATIENTS AND METHODS: A total of 182 patients with CD were studied: 63 patients were untreated; 50 patients were following a strict GFD; and 69 patients were non-compliant with a GFD. IgA-AAA was detected using a homemade enzyme-linked immunosorbent assay (ELISA). RESULTS: IgA-AAA showed a sensitivity of 41.3% and a specificity of 71.4% for a diagnosis of CD. In children, the frequency of IgA-AAA detection was lower in those following a strict GFD (23.1%) compared with untreated patients (39.4%) and those not complying with a GFD (32.5%). In patients following a strict GFD, IgA-AAA detection was significantly less frequent in children than in adults (23.1% vs. 58.3%, respectively; P<0.001). IgA-AAA was found in 17 out of 52 CD patients with total villous atrophy (32.7%), and in one out of 11 patients with subtotal villous atrophy (9%). CONCLUSION: IgA-AAA cannot replace anti-endomysium and anti-tissue transglutaminase antibodies in the diagnosis algorithm of CD, but it can serve as a reliable marker of severe intestinal mucosal damage in CD patients.


Assuntos
Actinas/imunologia , Autoanticorpos/sangue , Doença Celíaca/diagnóstico , Doença Celíaca/imunologia , Imunoglobulina A/sangue , Mucosa Intestinal/patologia , Adolescente , Adulto , Biomarcadores/sangue , Doença Celíaca/dietoterapia , Distribuição de Qui-Quadrado , Criança , Pré-Escolar , Dieta Livre de Glúten , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Cooperação do Paciente , Sensibilidade e Especificidade , Índice de Gravidade de Doença , Tunísia , Adulto Jovem
10.
Arch Virol ; 155(9): 1509-13, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20607319

RESUMO

The aims of our investigations were (1) to look for Aichi virus in environmental samples and (2) to compare the Aichi virus strains in both clinical and environmental samples in order to evaluate the role of environmental contamination as a possible vehicle for viral transmission. Aichi virus was detected in 15 (6%) sewage samples and in 4 (6.6%) shellfish samples. Aichi virus was identified for the first time in water samples. Phylogenetic analysis revealed several clusters that occurred sequentially in time, suggesting some parallelism in the evolution of environmental and human strains. Aichi virus present in sewage reflects the viruses circulating in the community.


Assuntos
Kobuvirus/genética , Kobuvirus/isolamento & purificação , Esgotos/virologia , Frutos do Mar/virologia , Animais , Humanos , Kobuvirus/classificação , Dados de Sequência Molecular , Filogenia , Infecções por Picornaviridae/virologia , Tunísia
11.
J Appl Microbiol ; 109(3): 1093-104, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20553345

RESUMO

AIMS: A prospective study was performed to characterize the main human enteric viruses able to persist in sewage samples and in shellfish tissues, and to establish the correlation between environmental strains and viral infantile diarrhoea observed in the same area during the same period. METHODS AND RESULTS: A total of 250 sewage (raw and treated) and 60 shellfish samples were collected between January 2003 and April 2007 in Monastir region, Tunisia. Group A rotavirus (RVA) was detected in 80 (32%) sewage samples, norovirus (NoV) in 11 (4·4%) and enteric adenovirus (AdV) in 1 (0·4%). Among 60 shellfish samples collected near sewage effluents, one was contaminated by NoV (1·6%). CONCLUSION: Our data represent the first documentation in Tunisia, combining gastroenteritis viruses circulating in the environment and in clinical isolates. We observed a correlation between environmental strains and those found in children suffering from gastroenteritis during the same period study. This suggests the existence of a relationship between water contamination and paediatric diarrhoea. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results address the potential health risks associated with transmission of human enteric viruses through water-related environmental routes. The research findings will aid in elucidating the molecular epidemiology and circulation of enteric viruses in Tunisia and in Africa, where data are rare.


Assuntos
Esgotos/virologia , Frutos do Mar/virologia , Vírus/isolamento & purificação , Adenoviridae/genética , Adenoviridae/isolamento & purificação , Animais , Criança , Gastroenterite/virologia , Humanos , Norovirus/genética , Norovirus/isolamento & purificação , Estudos Prospectivos , Rotavirus/genética , Rotavirus/isolamento & purificação , Tunísia , Vírus/classificação , Microbiologia da Água , Poluição da Água
12.
Lupus ; 18(8): 705-12, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19502266

RESUMO

Angiogenesis plays a critical role in the pathogenesis of several connective tissue diseases. There is, however, relatively little information available on the role of angiogenesis in systemic lupus erythematosus (SLE). The aim of this study was to investigate the angiogenic activity in sera of patients with SLE and to determine the association between angiogenic activity and clinical complications. Sera from 66 Tunisian females with SLE and from 32 healthy blood donors were studied for their angiogenic activity using the in-vitro tube formation test on Matrigel. Samples were divided into five groups according to their angiogenic activity, which was scored from 0 (no angiogenesis) to 4 (high angiogenic activity). Samples from each group were then tested randomly to assess serum concentration of vascular endothelial growth factor (VEGF). No correlation was found between angiogenic activity scores and serum VEGF levels. Considering angiogenesis assessment in-vitro, sera of patients with SLE showed a much higher angiogenic activity than healthy controls since a high angiogenic score (score 4) is present in 43.9% of patients and in 6.3% of controls (P < 0.0002). This high angiogenic activity is not correlated with disease activity; however, SLE patients with anti-dsDNA antibodies and those with nephritis showed higher angiogenic activity compared with patients without these complications since score 4 is found in 50.9% and 67.9% versus 9.1% (P = 0.017) and 26.3% (P < 0.001), respectively. In conclusion, our study showed that high serum angiogenic activity in SLE was not correlated with the VEGF levels. We suggest the use of the 'in-vitro' tube formation test as a better tool to study the angiogenic potential of sera. We found that in patients with SLE, serum angiogenic activity is increased compared with healthy controls. This high angiogenic activity is associated with renal complications and with the presence of anti-dsDNA antibodies. These findings suggest an involvement of angiogenesis disturbance in the pathogenesis of SLE.


Assuntos
Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/patologia , Neovascularização Patológica , Soro/metabolismo , Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Lúpus Eritematoso Sistêmico/fisiopatologia , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Tunísia , Fator A de Crescimento do Endotélio Vascular/sangue , Adulto Jovem
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