Combining multiple optical trapping with microflow manipulation for the rapid bioanalytics on microparticles in a chip.

An array of four independent laser traps is combined with a polydimethylsiloxane microfluidic chip to form a very compact system allowing parallel processing of biological objects. Strong three dimensional trapping allows holding objects such as functionalized beads in flows at speeds near 1 mm/s, enabling rapid processing. By pressure control of the inlet flows, the trapped objects can be put in contact with different solutions for analysis purpose. This setup, including a fluorescence excitation-detection scheme, offers the potential to perform complex biochemical manipulations on an ensemble of microparticles.

Spatially incoherent illumination as a mechanism for cross-talk suppression in wide-field optical coherence tomography.

Comparison of two illumination modes for wide-field optical coherence tomography has revealed that spatially coherent illumination generates coherent cross talk, causing significant image degradation, and that spatially incoherent illumination, with an adequate interferometer design, provides an efficient mechanism for suppression of coherent cross talk. This is shown by comparison of a pulsed laser with a thermal light source for a U.S. Air Force resolution target covered with a scattering solution made from microbeads as well as for an ex vivo tooth.

Self-referenced method for optical path difference calibration in low-coherence interferometry.

A simple method for the calibration of optical path difference modulation in low-coherence interferometry is presented. Spectrally filtering a part of the detected interference signal results in a high-coherence signal that encodes the scan imperfections and permits their correction. The method is self-referenced in the sense that no secondary high-coherence light source is necessary. Using a spectrometer setup for spectral filtering allows for flexibility in both the choice of calibration wavelength and the maximum scan range. To demonstrate the method's usefulness, it is combined with a recently published digital spectral shaping technique to measure the thickness of a pellicle beam splitter with a white-light source.

High-speed femtosecond pump-probe spectroscopy with a smart pixel detector array.

A new femtosecond pump-probe spectroscopy technique is demonstrated that permits the high-speed, parallel acquisition of pump-probe measurements at multiple wavelengths. This is made possible by use of a novel, two-dimensional smart pixel detector array that performs amplitude demodulation in real time on each pixel. This detector array can not only achieve sensitivities comparable with lock-in amplification but also simultaneously performs demodulation of probe transmission signals at multiple wavelengths, thus permitting rapid time- and wavelength-resolved femtosecond pump-probe spectroscopy. Measurements on a thin sample of bulk GaAs are performed across 58 simultaneous wavelengths. Differential probe transmission changes as small as approximately 2 x 10(-4) can be measured over a 5-ps delay scan in only approximately 3 min. This technology can be applied to a wide range of pump-probe measurements in condensed matter, chemistry, and biology.

Local coupling-coefficient characterization in fiber Bragg gratings.

We propose a new method for characterizing the local parameters of fiber Bragg gratings. This method combines measurement of the complex impulse response by optical low-coherence reflectometry and reconstruction of the complex coupling coefficient by layer peeling. Application of the method to a nonhomogeneous grating shows that the local coupling coefficient can be precisely determined with an axial resolution below 20 microm and a maximum error of less than 5% for amplitude and phase, respectively.

Continuous-wave broadband emitter based on a transition-metal-ion-doped waveguide.

We demonstrate the suitability of a simple continuous-wave-pumped transition-metal-ion-doped waveguide as a broadband light source in the wavelength region 600-1000 nm for interferometric applications. Spatially coherent (single mode in the confined direction), spectrally broadband (~130-nm FWHM) luminescence with output powers of several hundreds of microwatts is obtained from a Ti:sapphire planar waveguide with incident pump powers from an Ar-ion laser of up to 1 W. This result represents an increase in power by several orders of magnitude from previously reported simple broadband light sources in this wavelength range.

Optical coherence topography based on a two-dimensional smart detector array.

A low-coherence reflectometer based on a conventional Michelson interferometer and a novel silicon detector chip with a two-dimensional array of pixels that allows parallel heterodyne detection is presented. We demonstrate acquisition of three-dimensional images with more than 100,000 voxels per scan at a sensitivity of -58 dB and a rate of 6 Hz.

Video-rate optical low-coherence reflectometry based on a linear smart detector array.

A low-coherence reflectometer based on a conventional Michelson interferometer and a novel silicon detector chip that allows parallel heterodyne detection is presented. Cross-sectional images of 64x256 pixels covering an area of 1.92 mm x 1.3 mm are acquired at video rate and with a sensitivity close to the shot-noise limit. Applications in surface profiling and thickness measurement are demonstrated.

Temperature-related reversible birefringence changes in rat tail tendon.

By use of a highly sensitive method for measuring slight variations in birefringence it is shown here that a strong reversible correlation exists between rat tail tendon birefringence and temperature. This phenomenon is totally different from the loss of birefringence that results from a denaturation process. Below the threshold temperature leading to denaturation, an increase in temperature is systematically accompanied by a reversible increase in birefringence (0.25% degrees C(-1)). This phenomenon is observed at very fast heating rates (250,000 degrees C s(-1)), such as those induced by pulsed infrared lasers, and confirmed by experiments conducted with slow homogeneous heating of the sample medium (0.1 degrees C s(-1)). The good correlation between birefringence and temperature observed during the fast heating suggests that there are only small modifications of the tissue structure at the fibril level.

Stable and widely tunable all-fiber Pr(3+)-doped cw laser system using fiber Bragg gratings.

A simple and strictly all-fiber 1300-nm cw laser is presented. It is tunable over 16 nm with 0.5-nm linewidth and several milliwatts of output power in the whole tuning range from 1292 to 1308 nm. The setup uses a fiber Bragg grating as a combined tuning element and narrow-band output mirror. The simplicity and stability of this system are due to the following two characteristics: First, polarization control is not necessary anywhere in the system, and second, a wavelength-selective system and a narrow-band mirror are combined into one novel fiber-optic element.

Optical low-coherence reflectometry characterization of cladding modes excited by long-period fiber gratings.

The group refractive-index difference of cladding modes excited by a long-period fiber grating is characterized by use of the technique of optical low-coherence reflectometry, with a precision of <10(-4) . Very good agreement between theoretical and experimental results is demonstrated.

Precision and Reproducibility of Measurements of Human Corneal Thickness with Rapid Optical Low-Coherence Reflectometry (OLCR).

This study investigates the precision and intraindividual variability of a clinical optical pachometer based on low-coherence reflectometry, which was used to measure the central thickness of a human cornea in vivo. The instrument, attached to a slit lamp, is a single mode fiber optic based Michelson interferometer with a high repetition rate as previously described. The same operator performed ten sets of measurements on the same subject, each consisting of 20 consecutive scans, on each day for three consecutive days. By computing the means from every scan series, the thickness of the central cornea with optical pachometry was found to be 519.6±1.2 (range 518-521) µm on day 1, 519.9±0.9 (range 519-521) µm on day 2, and 523.8±0.6 (range 523-525) µm on day 3. The thickness values on day 3, where the subject suffered from a cold without clinical ocular involvement, were different from the two previous days (p<0.001, one way analysis of variance). Optical low-coherence reflectometry measurements of corneal thickness can be performed with high precision of about 1 µm and a high intra- and intersession reproducibility. © 1999 Society of Photo-Optical Instrumentation Engineers.

[Measuring corneal thickness in photo-keratectomy with a reflectometry incorporated into the laser beam of the excimer laser]. / Messung der Hornhautdicke bei der Photo-Keratektomie mit einem in den Strahlengang des Excimer-Lasers integrierten Reflektometer.

PURPOSE: To integrate a newly developed OLCR instrument into the optical system of the excimer laser. The instrument is designed to perform corneal pachymetry before, during, and after corneal photoablation and thus allow for a precise and continuous on-line measurement of the corneal photoablation process. METHODS: The conditions required to integrate the OLCR instrument into the excimer laser optics were investigated. With a technical setting providing on-line data of corneal thickness, three groups of 8-10 corneae received central keratectomies of 27 (group 1), 82 (group 2) and 163 (group 3) microns calculated central depth and 7.38 mm diameter. All measurements were performed with OLCR and ultrasound. RESULTS: The OLCR instrument was coupled into the optical system of the excimer laser and a useful signal obtained at SLD power levels of 40 microW incident on the cornea. Individual corneal thickness measurements were obtained before, during and after the photoablation procedure. In group 1, the ablation was 50.3 (40-68) microns measured with ultrasound and 30.2 (27-38) microns measured with OLCR. In group 2, the ablation was 101.1 (80-113) microns measured with ultrasound and 93.3 (76-109) microns measured with OLCR. In group 3, the ablation was 210.6 (190-227) microns measured with ultrasound and 188.4 (181-197) microns measured with OLCR. The precision (standard deviation) for measurements of individual corneas was 1-2 microns with OLCR and up to 12 mm in Ultrasound measurements. CONCLUSION: With this interferometric method, continuous, non-contact measurement of corneal thickness before, during and after excimer laser photoablation were performed. By establishing a feed-back control between the pachymetric measurements and the photoablation process, the precision of excimer ablation may possibly be further increased.

Continuous non-contact corneal pachymetry with a high speed reflectometer.

PURPOSE: We developed an instrument that permits non-contact, continuous, high speed and high precision monitoring of corneal thickness and tested the stability and reproducibility of measurements made over extended time periods and under various conditions of low surface reflectivity encountered during protracted exposure of the unmoistened corneal surface to ambient air. METHODS: The optical pachymeter (basic component of a broad-band, all-fiber Michelson interferometer) was used to monitor changes in the central corneal thickness of enucleated porcine eyes. Measurements were performed on three groups of eight eyes, each with different surface characteristics: intact epithelium, mechanically abraded epithelium, and 90 microm excimer laser keratectomy. Corneal thickness was monitored continuously with values recorded every 2 to 3 minutes for periods up to 1 hour in the absence of surface rinsing. RESULTS: The thicknesses of all unmoistened corneas could be monitored with a precision of 1 microm (ascertained using a calibration glass plate and a living human cornea) over the entire observation period. Under ambient air conditions, deturgescence occurred in each case, and ranged from 1 to 5 microm/min. The rate of corneal thinning was fairly constant during the first 15 minutes of monitoring, but was nonlinear thereafter. Corneas with an intact epithelium had the lowest thinning rate with only 10% of the original thickness lost during the course of 1 hour. Deturgescence increased to 25% in corneas that had mechanical removal of the epithelium and to 28.5% in those that had an anterior excimer laser keratectomy, during a similar time-period. CONCLUSION: With this new interferometric method, continuous, non-contact measurement of corneal thickness is possible to within a precision of 1 microm for periods up to 1 hour, even under the modified surface conditions after photoablative keratectomy. This device may be useful for on-line monitoring of ablation depths during such procedures.

Rapid and precise in vivo measurement of human corneal thickness with optical low-coherence reflectometry in normal human eyes.

An optical low-coherence reflectometer is used for rapid noncontact measurements of the human corneal thickness in vivo. Thickness measurements on ten volunteers show a standard deviation of 3.4 µm. The experiments reveal that the optical reflectometer benefits from a 2.5 fold enhancement of the measurement precision and a 2.8 fold reduction in measurement time compared to a standard clinical ultrasonic pachometer. © 1998 Society of Photo-Optical Instrumentation Engineers.

Fabrication of high-mechanical-resistance Bragg gratings in single-mode optical fibers with continuous-wave ultraviolet laser side exposure.

The mechanical resistance of single-mode fibers containing fiber Bragg gratings inscribed with cw UV laser irradiation is almost identical to that of pristine fiber. The median breaking strength of the gratings' Weibull distribution is more than 5 GPa, and the m value is of the order of 70. Based on a dynamic fatigue model, a Bragg grating lifetime of 50 years with a failure probability of 0.001 is predicted, assuming a constant applied stress of 0.96 GPa.

Efficient broadband intracore grating LP(01)-LP(02) mode converters for chromatic-dispersion compensation.

LP(01)-LP(02) mode converters based on UV-written intracore gratings have been fabricated for chromatic-dispersion compensation. The mode converters operate in transmission at wavelengths near 150 nm with spectral bandwidths of 14-25 nm and coupling efficiencies of as much as 90%. A large negative dispersion of -335 ps/(nm km) is obtained for a compensator consisting of the mode converter and a 1490-m-long few-mode fiber. The compensator shows negative dispersion over a wavelength region of 5 nm. Its figure of merit is estimated to be 370 ps/(nm dB).

Effects of drawing tension on the photosensitivity of Sn-Ge- and B-Ge-codoped core fibers.

The influence of drawing tension on the formation of Bragg gratings in B-Ge- and Sn-Ge-codoped core fibers is investigated by transmission and stress measurements. With increasing drawing tension, the axial stress is reduced in the B-Ge-codoped core but increased in the Sn-Ge-codoped core. A higher drawing tension leads to a photosensitivity enhancement and an increase of the core stress during the grating formation in B-Ge-codoped fibers. For Sn-Ge-codoped fibers, increased drawing tension results in an UV-induced stress decrease accompanied by a reduction of the photosensitivity. The results are discussed on the basis of an UV-induced compaction of the core network.

Rapid and scalable scans at 21 m/s in optical low-coherence reflectometry.

An optical low-coherence reflectometer is presented that uses a fiber Michelson interferometer with a rotating cube to generate rapid depth scans at a high repetition rate. A folded optical path geometry allows the reference arm to scale up the scan range, scan speed, and scan repetitiveness. Thickness measurements with a repetitiveness of 384 Hz and a longitudinal scanning speed of 21 m/s in air over a range of ~3 mm are demonstrated.

UV-irradiation-induced structural transformation of germanoscilicate glass fiber.

Raman spectra of germanosilicate core fibers before and after UV irradiation were investigated. Significant changes of the Raman spectra after irradiation indicate transformation of the glass structure. A possible interpretation of the observed changes is proposed.