Los trastornos de la personalidad ¿realidad o ficción? El sombrío futuro de su diagnóstico / Personality disorders: reality or fiction? The bleak future of your diagnosis

El diagnóstico categorial de los trastornos de la personalidad (TTPP) ha sido criticado por diversas razones, entras las que se encuentran el solapamiento de síntomas entre distintos trastornos, su elevada comorbilidad o el carácter dicotómico de su diagnóstico. Estas críticas han llevado al desarrollo de un planteamiento dimensional en las últimas versiones de los sistemas de clasificación, DSM-5/DSM-5-TR y CIE-11, considerando dos aspectos: el funcionamiento de la personalidad y una serie de rasgos patológicos. A pesar de la cuantiosa literatura publicada desde principio de este siglo sobre esta propuesta dimensional, no está claro, a día de hoy, que tenga alguna utilidad clínica. La vaga, abstracta y poco operativizable exposición de lo que constituye el funcionamiento de la personalidad y la compleja y forzada designación de rasgos mayores y menores (facetas) en el DSM-5/DSM-5-TR o sólo mayores y opcionales en la CIE-11, complican el diagnóstico dimensional de los TTPP. En este trabajo se discuten todas estas cuestiones en un intento de aportar algo de luz para un futuro menos sombrío que el actual panorama de los TTPP.(AU)

The categorical diagnosis of personality disorders (PDs) has been criticized forvarious reasons, among which are overlapping of symptoms between differentdisorders, their high comorbidity, and the dichotomous nature of their diagnosis.These criticisms have led to the development of a dimensional approach in thelatest versions of the classification systems, DSM-5/DSM-5-TR and ICD-11,considering two substantial aspects for its new diagnosis: personality functioning,and a series of pathological features. Despite the large amount of literature thathas been published since the beginning of this century on this dimensionalproposal, it is not clear, to date, that this approach enhances clinical utility. Thevague, abstract, and inoperative exposition of what constitutes personalityfunctioning and the complex and forced designation of major and minor traits(facets) in the DSM-5/DSM-5-TR, and only major and optional traits in the ICD-11,complicate, in an unusual way, the dimensional diagnosis of PDs. This paperdiscusses all of these issues in an attempt to shed some light on the potentially darkfuture of the current PDs panorama.(AU)

Medulloblastoma Development in a Patient with a Constitutional Balanced t(5;22)(q35.1;q11.2) Involving the NF2 Gene.

Neurofibromatosis type 2 (NF2) is a brain tumor predisposing syndrome caused by inactivating alterations of the NF2 gene mapped at chromosome 22q. Currently, no genetic information exists on medulloblastomas occurring in NF2 patients. We herein report on the genetic alterations observed in a girl in which the NF2 gene was de novo altered due to a constitutional translocation: t(5;22)(q35.1;q11.2). This girl had a particularly aggressive disease course. At the age of 4, she had already been diagnosed with three lesions classified as schwannomas and a meningioma. At 10 years old, she developed a medulloblastoma. She died at the age of 14 due to a refractory acute myeloid leukemia (AML). From the genetic point of view, we observed that (1) the NF2 gene was rearranged in all patient samples: blood, tumor, and leukemic cells; (2) loss of 3' region of NF2 and the downstream regions of chromosome 22 were only detected in medulloblastoma cells; (3) the known cancer AML-related gene: NPM1 which is mapped at 5q35.1 was not the target of any alteration in our patient. Our data suggest that inactivation of the NF2 gene was relevant for the medulloblastoma pathogenesis. Furthermore, we know that malignant cancers are the result of a multi-epi-genetic sequence of events, and although, unquestionably limited to the genetic findings in one case. We may hypothesize, that as described for a fraction of medulloblastomas, the alteration of a gene mapped at 5q might also have been relevant for medulloblastoma development in our patient.

Botrytis cinerea infection accelerates ripening and cell wall disassembly to promote disease in tomato fruit.

Postharvest fungal pathogens benefit from the increased host susceptibility that occurs during fruit ripening. In unripe fruit, pathogens often remain quiescent and unable to cause disease until ripening begins, emerging at this point into destructive necrotrophic lifestyles that quickly result in fruit decay. Here, we demonstrate that one such pathogen, Botrytis cinerea, actively induces ripening processes to facilitate infections and promote disease in tomato (Solanum lycopersicum). Assessments of ripening progression revealed that B. cinerea accelerated external coloration, ethylene production, and softening in unripe fruit, while mRNA sequencing of inoculated unripe fruit confirmed the corresponding upregulation of host genes involved in ripening processes, such as ethylene biosynthesis and cell wall degradation. Furthermore, an enzyme-linked immunosorbent assay (ELISA)-based glycomics technique used to assess fruit cell wall polysaccharides revealed remarkable similarities in the cell wall polysaccharide changes caused by both infections of unripe fruit and ripening of healthy fruit, particularly in the increased accessibility of pectic polysaccharides. Virulence and additional ripening assessment experiments with B. cinerea knockout mutants showed that induction of ripening depends on the ability to infect the host and break down pectin. The B. cinerea double knockout Δbc polygalacturonase1 Δbc polygalacturonase2 lacking two critical pectin degrading enzymes was incapable of emerging from quiescence even long after the fruit had ripened at its own pace, suggesting that the failure to accelerate ripening severely inhibits fungal survival on unripe fruit. These findings demonstrate that active induction of ripening in unripe tomato fruit is an important infection strategy for B. cinerea.

Aplicación internacional del programa “Intervención multidimensional para la ansiedad social” (IMAS): II. Eficacia del tratamiento para problemas relacionados con la ansiedad social / [International application of the program "Multidimensional intervention for social anxiety" (IMAS): II. Efficacy of treatment for problems related to social anxiety]

En un trabajo anterior (Caballo et al., 2021) comprobamos la eficacia del programa de Intervención multidimensional para la ansiedad social (IMAS) en la reducción de los síntomas de ansiedad social. En este estudio cuasiexperimental, con medidas pre/postratamiento y seguimiento, hallamos el impacto del programa IMAS en otros problemas relacionados con la ansiedad social. 57 personas diagnosticadas con un trastorno de ansiedad social (TAS), según el DSM-5, contestaron a cuestionarios que medían habilidades sociales, depresión, síntomas del trastorno de la personalidad por evitación, alcoholismo, autoestima, sensibilidad personal, preocupaciones y calidad de vida. Diferentes terapeutas llevaron a cabo el tratamiento en Ecuador, España y Paraguay. Los resultados mostraron importantes mejoras en el postratamiento en prácticamente todos los aspectos evaluados, mejoras que se mantenían a los seis meses. Los tamaños del efecto sobre la eficacia del tratamiento iban de medianos a grandes. Se comparó también el programa IMAS con terapia cognitivo conductual individual y tratamiento farmacológico, con resultados favorables para el programa IMAS. Este nuevo programa para el tratamiento de la ansiedad social tiene un impacto importante en otros problemas relacionados habitualmente con el TAS.

In a previous work (Caballo et al., 2021) we tested the effectiveness of the Multidimensional Intervention for Social Anxiety (MISA) program in reducing social anxiety symptoms. In this quasi-experimental study, with pre/post-treatment and follow-up measures, we examined the impact of the MISA program on other problems related to social anxiety. 57 people diagnosed with social anxiety disorder (SAD), according to DSM-5, were assessed with a diagnostic interview and questionnaires measuring social skills, depression, avoidant personality disorder symptoms, alcoholism, self-esteem, personal sensitivity, worries, and quality of life. Different therapists carried out the treatment in Ecuador, Spain, and Paraguay. The results showed significant improvements at post-treatment in virtually all measures assessing the above variables, improvements that were maintained at six months. Effect sizes on treatment effectiveness ranged from medium to large. The MISA program was also compared with individual cognitive behavioral therapy and pharmacological treatment, with favorable results for the MISA program. In conclusion, this new program for the treatment of social anxiety has a significant impact on other problems usually related to SAD.

Host susceptibility factors render ripe tomato fruit vulnerable to fungal disease despite active immune responses.

The increased susceptibility of ripe fruit to fungal pathogens poses a substantial threat to crop production and marketability. Here, we coupled transcriptomic analyses with mutant studies to uncover critical processes associated with defense and susceptibility in tomato (Solanum lycopersicum) fruit. Using unripe and ripe fruit inoculated with three fungal pathogens, we identified common pathogen responses reliant on chitinases, WRKY transcription factors, and reactive oxygen species detoxification. We established that the magnitude and diversity of defense responses do not significantly impact the interaction outcome, as susceptible ripe fruit mounted a strong immune response to pathogen infection. Then, to distinguish features of ripening that may be responsible for susceptibility, we utilized non-ripening tomato mutants that displayed different susceptibility patterns to fungal infection. Based on transcriptional and hormone profiling, susceptible tomato genotypes had losses in the maintenance of cellular redox homeostasis, while jasmonic acid accumulation and signaling coincided with defense activation in resistant fruit. We identified and validated a susceptibility factor, pectate lyase (PL). CRISPR-based knockouts of PL, but not polygalacturonase (PG2a), reduced susceptibility of ripe fruit by >50%. This study suggests that targeting specific genes that promote susceptibility is a viable strategy to improve the resistance of tomato fruit against fungal disease.

Aplicación internacional del programa “Intervención multidimensional para la ansiedad social” (IMAS): I. Eficacia del tratamiento en pacientes con ansiedad social / International application of the program Multidimensional intervention for social anxiety

El trastorno de ansiedad social (TAS) es uno de los trastornos más frecuentes a nivel mundial. El objetivo de este estudio fue comprobar la eficacia del nuevo programa “Intervención multidimensional para la ansiedad social” (IMAS) para el tratamiento del TAS. Participaron 57 personas diagnosticadas con TAS, según el DSM-5, evaluadas mediante una entrevista semiestructurada (Salazar y Caballo, 2018) y dos medidas de autoinforme para la ansiedad social, el CASO (Caballo, Salazar, Arias, et al., 2010) y la LSAS-SR (Liebowitz, 1987). Diferentes terapeutas llevaron a cabo el tratamiento en Ecuador, España y Paraguay. Los resultados mostraron importantes mejoras del pretratamiento al postratamiento, que se mantenían a los seis meses. El tamaño del efecto estaba entre 1 y 2 y, en muchas ocasiones, fue superior a 2. Aunque se comparó con un grupo de terapia cognitivo conductual y otro de tratamiento farmacológico, con resultados favorables para el programa IMAS, el bajo número de sujetos de estos últimos grupos no permite llegar a deducciones claras. En conclusión, este nuevo programa para el tratamiento de la ansiedad social parece altamente eficaz a corto y medio plazo y sus resultados positivos parecen generalizables a diferentes países (AU)

Social anxiety disorder (SAD) is one of the most prevalent disorders worldwide. The goal of this study was to test the effectiveness of the new program "Multidimensional Intervention for Social Anxiety" (MISA) for the treatment of SAD. Fifty-seven people diagnosed with SAD, according to the DSM-5, participated in this study, and they were assessed by means of a semi-structured interview (Salazar & Caballo, 2018) and two self-report measures for social anxiety, the SAQ (Caballo, Salazar, Arias, et al., 2010) and the LSAS-SR (Liebowitz, 1987). Different therapists delivered the treatment in Ecuador, Spain, and Paraguay. The results showed significant improvements from pre-treatment to post-treatment, which were maintained at six months. The effect size was between 1 and 2 and, on many occasions, was greater than 2. Although it was compared with a cognitive behavioral therapy group and a pharmacological treatment group, with favorable results for the MISA program, the low number of subjects in the latter groups does not allow clear deductions to be made. In conclusion, this new program for the treatment of social anxiety seems highly effective in the short and medium term and its positive results seem generalizable to different countries (AU)

Propiedades psicométricas del Cuestionario de ansiedad social para adultos (CASO) en Perú / Psychometric properties of the Social Anxiety Questionnaire for Adults (SAQ) in Peru

Resumen La investigación sobre la evaluación de la ansiedad social en Iberoamérica es escasa. En los últimos años se ha informado sobre las características psicométricas del "Cuestionario de ansiedad social para adultos" (CASO) en distintos países iberoamericanos, excepto Perú. El objetivo de este estudio fue analizar las pruebas de validez basadas en el constructo, las pruebas de validez basadas en la relación con otras variables de tipo convergente y la fiabilidad del CASO, utilizando una muestra no clínica de 3064 peruanos. El análisis factorial confirmatorio comprobó que el modelo de cinco factores relacionados se ajustaba mejor a los datos que el modelo jerárquico y se halló que el modelo unifactorial no tenía un buen ajuste. Las correlaciones entre los factores del CASO (de .608 a .709) indicaron que cada uno de ellos evalúa aspectos específicos del constructo y aportan, a su vez, a la medición global del mismo. Los coeficientes de fiabilidad fueron de aceptables a muy buenos (.78( (( .85, .75( (( .83). La alta correlación (r= .69) con la subescala de Ansiedad de la "Escala de ansiedad social de Liebowitz, versión de autoinforme" (LSAS-SR), apoya su relación con la variable convergente. Estas adecuadas características psicométricas del CASO respaldan su utilización con población peruana.

Abstract Research on the assessment of social anxiety in Ibero-America is scarce. In recent years, the psychometric characteristics of the Social Anxiety Questionnaire for adults (SAQ) have been reported in different Ibero-American countries except Peru. The aim of this study was to analyze the construct and the convergent validity, and the reliability of the SAQ, using a non-clinical sample of 3064 Peruvians. The confirmatory factorial analysis showed that the model of five-related factors was better adjusted to the data than the hierarchical model, and that the unifactorial model did not have a good fit. The correlations between their factors (from .608 to .709) indicate that each of them evaluates specific aspects of the construct and also contribute to the overall measurement of it. The reliability coefficients were from acceptable to very good (.78( (( .85, .75( (( .83). The high correlation (r= .69) with the Anxiety subscale of the "Liebowitz Social Anxiety Scale-Self-Report version" (LSAS-SR) supports its convergent validity. These appropriate psychometric characteristics of the questionnaire support its use with the Peruvian population.

CX3CL1-CX3CR1 Axis: A New Player in Coeliac Disease Pathogenesis.

BACKGROUND: The CX3CL1-CX3CR1 axis has been related to numerous diseases. The aim of our study was to investigate its involvement in coeliac disease (CD) pathogenesis, particularly in the early phase of the disease. METHODS: We collected peripheral blood from CD patients and controls, enrolled in a 3-day gluten challenge, to study soluble CX3CL1, I-TAC and MIG by Luminex, CX3CL1 and CX3CR1 gene expression by qPCR, and CX3CR1 protein expression in monocytes and CD8+, CD4+ and γδ+ T cells, by flow cytometry. We also analysed the expression of the CX3CL1 and CX3CR1 mRNA and protein in the duodenal biopsies of CD patients with active and treated disease, and in non-CD control individuals, by qPCR and immunohistochemistry. RESULTS: After the gluten challenge, increased levels of CX3CL1, I-TAC and MIG proteins were observed in the peripheral blood of CD patients, with no changes in CX3CL1 mRNA, or CX3CR1 mRNA and protein. Regarding duodenal tissue, CX3CL1 was absent or barely present in the superficial and basal epithelium of CD patients, contrasting with the moderate to high levels present in controls. CONCLUSIONS: CX3CL1 seems to be involved in the appearance and progression of CD, and it appears to be a potential diagnostic biomarker. Its use as an alternative therapeutic target in CD deserves further research.

Evaluation of the Reliability and Validity of the Social Anxiety Questionnaire for Children in Adolescents with Social Anxiety Disorder.

Little research has focused on the measurement of specific facets of social anxiety disorder (SAD) in adolescents. In this study, we report on the Social Anxiety Questionnaire for Children (SAQ-C; Caballo et al., 2016), a 24-item questionnaire which assesses six facets of social anxiety in youth: 1) Speaking in public/Interactions with teachers, 2) Interactions with the opposite sex, 3) Criticism and embarrassment, 4) Assertive expression of annoyance, disgust, or displeasure, 5) Interactions with strangers, and 6) Performing in public. The SAQ-C has been shown to have sound psychometric properties in large samples of non-clinical Latin-American and Spanish youth. The present study aimed to provide the first evaluation of the psychometric properties of the SAQ-C in a clinical sample of 58 English-speaking adolescents diagnosed with SAD in the United States. Findings support the reliability and validity of this new measure and reveal specific facets of social anxiety not adequately captured in other frequently used self- report measures. Implications of the findings for assessment and treatment are addressed.

A new Multidimensional Intervention for Social Anxiety: The MISA Program / Una nueva Intervención multidimensional para la ansiedad social: el programa IMAS

This article presents a new treatment program for social anxiety, specifically for social anxiety disorder (social phobia) and avoidance personality disorder. Its name, Multidimensional Intervention for Social Anxiety (MISA), indicates that it revolves around the five dimensions that our research team found working on social anxiety with participants from 20 countries for more than 15 years, namely (1) interaction with strangers, (2) interaction with the opposite sex, (3) assertive expression of annoyance, disgust, or displeasure, (4) embarrassment or criticism, and (5) speaking in public/interaction with persons of authority. It incorporates both traditional techniques of cognitive behavioral therapy and strategies of third-generation therapies. The MISA program is applied in a group format through 15 treatment sessions of 2.5 hours each, plus one booster session, and pre/post-treatment and follow-up assessment sessions. We believe that it is currently the best treatment program available for social anxiety and in the coming months we will be able to substantiate this claim

Se presenta un nuevo programa de tratamiento para la ansiedad social, específicamente para el trastorno de ansiedad social (fobia social) y el trastorno de personalidad por evitación. Su nombre, Intervención multidimensional para la ansiedad social (IMAS), indica que gira alrededor de las cinco dimensiones que nuestro equipo de investigación encontró trabajando en ansiedad social con 20 países durante más de 15 años, como son: 1) Interacción con desconocidos, 2) Interacción con el sexo opuesto, 3) Expresión asertiva de molestia, desagrado o enfado, 4) Quedar en evidencia/Hacer el ridículo, y 5) Hablar en público/Interacción con personas de autoridad. Incorpora tanto técnicas tradicionales de la terapia cognitivo conductual como estrategias de las terapias de tercera generación. El programa IMAS se aplica en formato grupal a lo largo de 15 sesiones de tratamiento de 2,5 horas cada una, más una sesión de apoyo y las sesiones de evaluación pre/postratamiento y seguimiento. Creemos que en estos momentos constituye el mejor programa de tratamiento para la ansiedad social y en los próximos meses estaremos en disposición de demostrar esta afirmación

Psychometric properties of the Liebowitz Social Anxiety Scale in a large cross-cultural Spanish and Portuguese speaking sample

Objective: To examine the psychometric properties of the Liebowitz Social Anxiety Scale-Self Report (LSAS-SR) based on a large sample recruited from 16 Latin American countries, Spain, and Portugal. Methods: Two groups of participants were included: a non-clinical sample involving 31,243 community subjects and a clinical sample comprising 529 patients with a diagnosis of social anxiety disorder (SAD). Exploratory factor analysis (EFA), confirmatory factor analysis (CFA) and exploratory structural equation modeling (ESEM) were used in order to determine the psychometric properties of the LSAS-SR. Results: EFA identified five factors with eigenvalues greater than 1.00 explaining 50.78% of the cumulative variance. CFA and ESEM supported this 5-factor structure of the LSAS-SR. The factors included: 1) speaking in public; 2) eating/drinking in front of other people; 3) assertive behaviors; 4) working/writing while being observed; and 5) interactions with strangers. Other psychometric properties such as inter-factor correlations, invariance, reliability, and validity of the scale were also found. Conclusion: Psychometric data support the internal consistency and convergent validity of the LSAS-SR. It seems to be a valid and reliable measure of global social anxiety for Spanish and Portuguese-speaking countries, although when considering a multidimensional approach (factor-based assessment) it seems to be lacking some relevant social situations that are feared in those countries.

Characterization of CRISPR Mutants Targeting Genes Modulating Pectin Degradation in Ripening Tomato.

Tomato (Solanum lycopersicum) is a globally important crop with an economic value in the tens of billions of dollars, and a significant supplier of essential vitamins, minerals, and phytochemicals in the human diet. Shelf life is a key quality trait related to alterations in cuticle properties and remodeling of the fruit cell walls. Studies with transgenic tomato plants undertaken over the last 20 years have indicated that a range of pectin-degrading enzymes are involved in cell wall remodeling. These studies usually involved silencing of only a single gene and it has proved difficult to compare the effects of silencing these genes across the different experimental systems. Here we report the generation of CRISPR-based mutants in the ripening-related genes encoding the pectin-degrading enzymes pectate lyase (PL), polygalacturonase 2a (PG2a), and ß-galactanase (TBG4). Comparison of the physiochemical properties of the fruits from a range of PL, PG2a, and TBG4 CRISPR lines demonstrated that only mutations in PL resulted in firmer fruits, although mutations in PG2a and TBG4 influenced fruit color and weight. Pectin localization, distribution, and solubility in the pericarp cells of the CRISPR mutant fruits were investigated using the monoclonal antibody probes LM19 to deesterified homogalacturonan, INRA-RU1 to rhamnogalacturonan I, LM5 to ß-1,4-galactan, and LM6 to arabinan epitopes, respectively. The data indicate that PL, PG2a, and TBG4 act on separate cell wall domains and the importance of cellulose microfibril-associated pectin is reflected in its increased occurrence in the different mutant lines.

Psychometric properties of the Liebowitz Social Anxiety Scale in a large cross-cultural Spanish and Portuguese speaking sample.

OBJECTIVE: To examine the psychometric properties of the Liebowitz Social Anxiety Scale-Self Report (LSAS-SR) based on a large sample recruited from 16 Latin American countries, Spain, and Portugal. METHODS: Two groups of participants were included: a non-clinical sample involving 31,243 community subjects and a clinical sample comprising 529 patients with a diagnosis of social anxiety disorder (SAD). Exploratory factor analysis (EFA), confirmatory factor analysis (CFA) and exploratory structural equation modeling (ESEM) were used in order to determine the psychometric properties of the LSAS-SR. RESULTS: EFA identified five factors with eigenvalues greater than 1.00 explaining 50.78% of the cumulative variance. CFA and ESEM supported this 5-factor structure of the LSAS-SR. The factors included: 1) speaking in public; 2) eating/drinking in front of other people; 3) assertive behaviors; 4) working/writing while being observed; and 5) interactions with strangers. Other psychometric properties such as inter-factor correlations, invariance, reliability, and validity of the scale were also found. CONCLUSION: Psychometric data support the internal consistency and convergent validity of the LSAS-SR. It seems to be a valid and reliable measure of global social anxiety for Spanish and Portuguese-speaking countries, although when considering a multidimensional approach (factor-based assessment) it seems to be lacking some relevant social situations that are feared in those countries.

Expression patterns common and unique to ulcerative colitis and celiac disease.

Autoimmune diseases like celiac disease (CeD) and ulcerative colitis (UC) show a common genetic background defined by the existence of shared susceptibility loci. We aimed to go deeper into this common genetic background through performing a cross-disease study based on gene expression. We measured the expression of 21 genes located in 13 CeD-UC susceptibility regions, and 10 genes in five CeD risk regions. Determinations were carried out in colon/rectum samples from 13 UC patients (inflamed and uninflamed tissue) and four colon samples from controls. Duodenal samples from 19 CeD patients and 12 controls were used for comparisons. Differences were analyzed using the Bayesian method. The shared chromosomal regions containing TNFAIP3, PTPN2, ICOSLG, C1orf106, and IL21 showed similar results in both diseases. FASLG, PLEK, CCR4, and TAGAP, all located in CeD risk loci, were up-regulated in both CeD and UC patients. Finally, ZFP36L1, ZMIZ1, PUS10, UBE2L3, and BACH2 showed opposite results in CeD and UC. A high complexity underlies autoimmune common susceptibility loci, as the expression pattern of the studied genes does not always correlate with the one expected attending to the apparent genetic background. Differentially expressed genes such as ZFP36L1, ZMIZ1, PUS10, and BACH2 deserve further research in autoimmune diseases.

Evaluation of T cells in blood after a short gluten challenge for coeliac disease diagnosis.

BACKGROUND AND AIM: To diagnose coeliac disease (CD) in individuals on a gluten free diet (GFD), we aimed to assess the utility of detecting activated γδ and CD8 T cells expressing gut-homing receptors after a short gluten challenge. METHODS: We studied 15 CD patients and 35 non-CD controls, all exposed to three days of gluten when following a GFD. Peripheral blood was collected before and six days after starting gluten consumption, and the expression of CD103, ß7 and CD38 in γδ and CD8 T cells was assessed by flow cytometry. Determination of IFN-γ and IP-10 was performed by means of ELISPOT and/or Luminex technology. RESULTS: We observed both γδ and CD8 T cells coexpressing CD103, ß7hi and CD38 in every patient with CD on day six, but only in one control. The studied CD8 T subpopulation was easier to detect than the γδ subpopulation. Increased IFN-γ and IP-10 levels after challenge were observed in patients with CD, but not in controls. CONCLUSION: A short three-day gluten challenge elicits the activation of CD103+ ß7hi CD8+ T cells in CD. These cells can be detected by flow cytometry in peripheral blood, opening new possibilities for CD diagnosis in individuals on a GFD.

La autoestima y su relación con la ansiedad social y las habilidades sociales / Self-esteem and its relationship with social anxiety and social skills

Este trabajo examina la autoestima y sus dimensiones utilizando la "Escala Janis-Field de sentimientos de inadecuación-Revisada" (RJFFIS) y la "Escala Rosenberg de autoestima" (RSES) en una muestra mayoritariamente universitaria (n= 826). Analizamos su relación con la ansiedad social (AS) y las habilidades sociales (HHSS), evaluadas con el "Cuestionario de ansiedad social para adultos" (CASO), el "Cuestionario de habilidades sociales" (CHASO) y el "Inventario de asertividad de Rathus" (RAS). La solución heptafactorial de la RJFFIS explica el 59,65% de la varianza común y apoya un concepto multifactorial de la autoestima, incluyendo facetas que tienen que ver con la apariencia y habilidades físicas, relaciones sociales y habilidades académicas o laborales. Las correlaciones moderadas con la RSES apoyan la validez convergente de la RJFFIS. Por otra parte, la autoestima se relaciona de forma moderada con la AS y las HHSS. Los sujetos con alta AS muestran una autoestima significativamente más baja que aquellos con baja AS y lo opuesto ocurre respecto a las HHSS. Existen diferencias de sexo en cuanto a la autoestima, mostrando los hombres puntuaciones más altas que las mujeres. Estos resultados sustentan la naturaleza multidimensional de la autoestima y la necesidad de investigar su papel en las áreas tanto de la AS como de las HHSS

This paper examines self-esteem and its dimensions using the Revised Janis-Field Feelings of Inadequacy Scale (RJFFIS) and the Rosenberg Self-Esteem Scale (RSES) in a sample comprising mostly university undergraduates (n= 826). We analyzed their relationship with social anxiety (SA) and social skills (SS), assessed with the Social Anxiety Questionnaire for adults (SAQ), the Social Skills Questionnaire (CHASO) and the Rathus Assertiveness Schedule (RAS). The heptafactorial solution for the RJFFIS explains 59.65% of the common variance and supports a multifactorial concept of self-esteem, including facets that have to do with physical appearance and abilities, social relationships, and academic or work skills. The moderate correlations with the RSES support the convergent validity of the RJFFIS. On the other hand, self-esteem is moderately related to SA and SS. Subjects with high SA show significantly lower self-esteem than those with a low SA and the opposite occurs regarding SS. There are gender differences in terms of self-esteem, with men showing higher scores than women. These results support the multidimensional nature of self-esteem and the need to investigate its role in the areas of both SA and SS

Validação brasileira do questionário de ansiedade social para adultos (CASO) / Brazilian validation of the social anxiety questionnaire for adults (SAQ) / Validación brasileña del cuestionario de ansiedad social para adultos (CASO)

Uma prática comum no campo da avaliação da ansiedade social em países de língua espanhola e portuguesa é o uso de instrumentos de origem anglo-saxã sem adaptação prévia para o país de nova aplicação. Recentemente, desenvolveu-se empiricamente o novo Questionário de Ansiedade Social para Adultos (CASO) com situações sociais provenientes da América Latina, da Espanha e de Portugal. Este artigo apresenta as propriedades psicométricas do CASO aplicado a uma amostra de 2.422 brasileiros. Os resultados mostram que o questionário mantém a sua estrutura original de cinco dimensões, com os seus 30 itens apresentando carga fatorial superior a 0,40 em cada um dos cinco fatores. A confiabilidade e a consistência interna são elevadas. Foram encontradas diferenças associadas ao sexo, com mulheres pontuaram de maneira mais elevada em três das cinco dimensões e na ansiedade social geral. Esses dados indicam que o CASO (SAQ) é uma medida válida e confiável para uso no Brasil.

A common practice in the field of social anxiety assessment in Portuguese and Spanish-speaking countries is the use of instruments, developed in English-speaking countries, without prior adaptation to the country of application. Recently, it has been empirically developed the new Social Anxiety Questionnaire for Adults (SAQ) with social situations from Ibero America, Spain, and Portugal. This article presents the psychometric properties of the SAQ, applied to a sample of 2,422 Brazilians. The results show that the questionnaire maintains its five-factor original structure, with its 30 items loading above 0.40 in each of the five factors. The reliability and internal consistency are high. Differences associated with gender were found, showing that women scored higher than men in three out of the five factors and in global social anxiety. These data indicate that the SAQ is a reliable and valid measure for use in Brazil.

Una práctica frecuente en el campo de la evaluación de la ansiedad social en países de lengua española y portuguesa es el empleo de instrumentos de origen anglosajón. Recientemente se ha desarrollado de forma empírica el nuevo Cuestionario de ansiedad social para adultos (CASO) con situaciones sociales generadas en Latinoamérica, España y Portugal. Este artículo presenta las propiedades psicométricas del CASO aplicado a una muestra de 2.422 brasileños. Los resultados muestran que el cuestionario mantiene su estructura original de cinco dimensiones, con sus 30 ítems presentando una saturación factorial superior a 0,40 en cada uno de los cinco factores. La fiabilidad y la consistencia interna son elevadas. Se encontraron diferencias asociadas al sexo, con las mujeres puntuando más alto que los hombres en tres de las cinco dimensiones y en la ansiedad social global. Estos datos indican que el CASO (SAQ) es una medida válida y fiable para su utilización en Brasil.

TRANSRECTAL ULTRASONOGRAPHY OF EQUINE LUMBOSACRAL NERVES: PILOT STUDY IN 28 HEALTHY WARMBLOOD HORSES.

A noninvasive method for visualizing lumbosacral nerves would be helpful for horses with suspected lumbosacral plexopathy or injury. The aim of this prospective descriptive pilot study was to characterize the ultrasonographic appearance of the lumbosacral nerves in a sample of healthy horses, and expand on the technique for image acquisition. Horses were recruited for inclusion if they were determined to be healthy and sound based on clinical and lameness evaluation. Transrectal ultrasound images of the lumbosacral nerves (L6, S1, and S2) were obtained for both sides. Landmarks for localization, and techniques for nerve identification and measurement were described. Effects of sex, age, side, and nerve on measured thickness were statistically tested. Twenty-eight warmblood horses were sampled (15 males and 13 females). Ages ranged from 5 to 15 years. Ventral nerve roots from L6 to S2 appeared as tubular structures with a characteristic hyperechoic linear echo pattern. There was no significant difference in nerve vertical diameter between left and right sides. A three-way interaction was found among sex, age and lumbosacral nerve. The L6 nerve in males was significantly larger than S1 only in the youngest group. The S2 nerve was significantly smaller than L6 or S1 regardless of age group or sex. In conclusion, transrectal ultrasound was a feasible method for visualizing and measuring equine lumbosacral nerves. Wide ranges of sizes for each nerve were found between horses, however nerves were bilaterally symmetrical within horses. Side comparison is therefore recommended when nerve pathology is suspected.

Desarrollo y validación de un nuevo instrumento para la evaluación de las habilidades sociales: El "Cuestionario de Habilidades Sociales" (CHASO) / Development and validation of a new social skills assessment instrument: The Social Skills Questionnaire (CHASO)

La evaluación y entrenamiento de las habilidades sociales constituye un área de permanente actualidad tanto en la clínica como en la investigación. No obstante, la evaluación de las habilidades sociales ha sido, desde sus comienzos, un área difícil de abordar. Un problema actual es que aún hoy día se siguen utilizando profusamente instrumentos de autoinforme de las habilidades sociales publicados en la década de los años 70. El objetivo principal de este estudio ha sido desarrollar un nuevo cuestionario que evaluase las principales dimensiones o clases de respuesta que comprende el constructo de las habilidades sociales. La tercera versión del «Cuestionario de habilidades sociales» (CHASO-III), formada por 76 ítems, se aplicó a 826 participantes de diversas provincias españolas. El análisis factorial exploratorio arrojó 10 factores coherentes y con escaso solapamiento entre ellos. Se seleccionaron los mejores cuatro ítems por factor, con el fin de establecer la versión final del cuestionario, el CHASO. Las propiedades psicométricas obtenidas para esta versión final, incluyendo su consistencia interna, su fiabilidad y su validez, fueron muy adecuadas, ofreciendo, así, un nuevo y actualizado instrumento de autoinforme de las habilidades sociales

The assessment and training of social skills constitutes a continuous hot topic both in clinic and research settings. However, social skills assessment has been since its beginnings a difficult area to address. One ongoing issue is that even today self-report instruments for social skills published in the 1970s are still profusely used. The main objective of this study has been to develop a new questionnaire to assess the main dimensions or response classes included in the construct of social skills. The third version of the Social Skills Questionnaire (CHASO-III), comprising 76 items, was administered to 826 participants from various Spanish regions. The exploratory factor analysis yielded 10 coherent factors with little overlap between them. The best four items from each factor were selected in order to establish the final version of the CHASO. The psychometric properties obtained for this final version, including its internal consistency, its reliability and its validity, were very adequate, thus offering a new and updated social skills self-report instrument

Validez de constructo y fiabilidad del «Cuestionario de ansiedad social para adultos¼ (CASO) en Colombia / Construct validity and reliability of the "Social Anxiety Questionnaire for adults" (SAQ) in Colombia

El «Cuestionario de ansiedad social para adultos¼ (CASO) es una medida de autoinforme desarrollada para los países de habla española y portuguesa. El objetivo de este estudio fue analizar la validez de constructo y la fiabilidad del CASO en Colombia. Participaron 5477 personas de la población general contestando el CASO y la «Escala de ansiedad social de Liebowitz-versión de autoinforme¼ (LSAS-SR). El análisis paralelo y el análisis factorial exploratorio del CASO arrojaron una solución de cinco factores (hablar en público/interacción con personas de autoridad; quedar en evidencia o en ridículo; expresión asertiva de molestia, desagrado o enfado; interacción con el sexo opuesto e interacción con desconocidos), que explicaban el 53.44% de la varianza. El análisis factorial confirmatorio mostró que el modelo de cinco factores correlacionados era el modelo con mejor ajuste. Las correlaciones entre la puntuación total del CASO y las subescalas y la puntuación total de la LSAS-SR fueron de moderadas a altas (0.53< r <0.64). La fiabilidad y la consistencia interna de la puntuación total del CASO fueron de 0.91 y 0.93, respectivamente, y para las dimensiones estuvieron entre 0.74 y 0.84. Estos resultados permiten concluir que el CASO es una medida válida y fiable para medir la ansiedad social en la población colombiana.

The Social Anxiety Questionnaire for adults (SAQ) is a self-report measurement tool developed for Portuguese and Spanish-speaking countries. The aim of this study was to examine the construct validity and reliability of the SAQ in Colombia. A total of 5477 people from the general population answered the SAQ and the Liebowitz Social Anxiety Scale, Self-Report version (LSAS-SR). The parallel and exploratory factor analysis of the SAQ yielded a five-factor solution with, speaking in public/talking with people in authority; criticism and embarrassment; assertive expression of annoyance, disgust, or displeasure; interaction with the opposite sex, and interactions with strangers, explaining 53.44% of variance. Confirmatory factor analysis showed that the five correlated factors model was the best fitted model. Correlations between the total score of the SAQ and total score of the LSAS-SR and its subscales were from moderate to high (0.53 < r< 0.64). The reliability and internal consistency for the total score and dimensions of the SAQ were 0.91 and 0.93, respectively, and for the dimensions were between 0.74 and 0.84. These results suggest that the SAQ is a valid and reliable measurement tool for the Colombian population.