Assuntos
Pérnio/terapia , Transplante de Células-Tronco Hematopoéticas , Imunossupressores/uso terapêutico , Leucemia Mieloide Aguda/terapia , Lúpus Eritematoso Discoide/terapia , Pancitopenia/terapia , Pérnio/etiologia , Pré-Escolar , Glucocorticoides/uso terapêutico , Humanos , Leucemia Mieloide Aguda/complicações , Lúpus Eritematoso Discoide/complicações , Masculino , Pancitopenia/etiologia , Transplante HomólogoRESUMO
Acute Graft-versus-host disease (GVHD) is a major immunological complication after allogeneic hematopoietic cell transplantation and a better understanding of the molecular regulation of the disease could help to develop novel targeted therapies. Here we found that a G/C polymorphism within the human microRNA-146a (miR-146a) gene of transplant recipients, which causes reduced miR-146a levels, was strongly associated with the risk of developing severe acute GVHD (n=289). In mice, deficiency of miR-146a in the hematopoietic system or transfer of recipient-type miR-146a-/- dendritic cells (DCs) enhanced GVHD, while miR-146a mimic-transfected DCs ameliorated disease. Mechanistically, lack of miR-146a enhanced JAK2-STAT1 pathway activity, which led to higher expression of class II-transactivator (CIITA) and consecutively increased MHCII-levels on DCs. Inhibition of JAK1/2 or CIITA knockdown in DCs prevented miR-146a-/- DC-induced GVHD exacerbation. Consistent with our findings in mice, patients with the miR-146a polymorphism rs2910164 in hematopoietic cells displayed higher MHCII levels on monocytes, which could be targeted by JAK1/2 inhibition. Our findings indicate that the miR-146a polymorphism rs2910164 identifies patients at high risk for GVHD before allo-HCT. Functionally we show that miR-146a acts as a central regulator of recipient-type DC activation during GVHD by dampening the pro-inflammatory JAK-STAT/CIITA/MHCII axis, which provides a scientific rationale for early JAK1/2 inhibition in selected patients.
Assuntos
Células Dendríticas/metabolismo , Expressão Gênica , Genes MHC da Classe II , Janus Quinases/metabolismo , MicroRNAs/genética , Fatores de Transcrição STAT/metabolismo , Transdução de Sinais , Animais , Estudos de Casos e Controles , Células Dendríticas/imunologia , Doença Enxerto-Hospedeiro/diagnóstico , Doença Enxerto-Hospedeiro/etiologia , Camundongos , Camundongos Knockout , Polimorfismo de Nucleotídeo Único , Índice de Gravidade de Doença , Transplante de Células-Tronco/efeitos adversosRESUMO
The gene PIK3CD codes for the catalytic subunit of phosphoinositide 3-kinase δ (PI3Kδ), and is expressed solely in leucocytes. Activating mutations of PIK3CD have been described to cause an autosomal dominant immunodeficiency that shares clinical features with common variable immunodeficiency (CVID). We screened a cohort of 669 molecularly undefined primary immunodeficiency patients for five reported mutations (four gain-of-function mutations in PIK3CD and a loss of function mutation in PIK3R1) using pyrosequencing. PIK3CD mutations were identified in three siblings diagnosed with CVID and two sporadic cases with a combined immunodeficiency (CID). The PIK3R1 mutation was not identified in the cohort. Our patients with activated PI3Kδ syndrome (APDS) showed a range of clinical and immunological findings, even within a single family, but shared a reduction in naive T cells. PIK3CD gain of function mutations are more likely to occur in patients with defective B and T cell responses and should be screened for in CVID and CID, but are less likely in patients with a pure B cell/hypogammaglobulinaemia phenotype.
Assuntos
Classe I de Fosfatidilinositol 3-Quinases/genética , Classe I de Fosfatidilinositol 3-Quinases/metabolismo , Imunodeficiência de Variável Comum/genética , Síndromes de Imunodeficiência/genética , Mutação , Adolescente , Adulto , Agamaglobulinemia/diagnóstico , Agamaglobulinemia/genética , Agamaglobulinemia/imunologia , Linfócitos B/imunologia , Criança , Imunodeficiência de Variável Comum/imunologia , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Síndromes de Imunodeficiência/imunologia , Imunofenotipagem , Masculino , Pessoa de Meia-Idade , Irmãos , Linfócitos T/imunologia , Adulto JovemRESUMO
Common variable immunodeficiency (CVID) encompasses a heterogeneous group of antibody deficiencies characterized by susceptibility to recurrent infections and sequelae, including bronchiectasis. We investigated the relevance of the lectin complement pathway in CVID patients by analysing ficolin-2 and ficolin-3 serum levels and genotyping single nucleotide polymorphisms (SNPs) in the FCN2 and FCN3 genes. Our results show that ficolin-2 levels in CVID patients are significantly lower (P < 0.0001) than in controls. The lowest ficolin-2 levels are found in CVID patients with bronchiectasis (P = 0.0004) and autoimmunity (P = 0.04). Although serum levels of ficolin-3 were similar in CVID patients and controls, CVID patients with bronchiectasis again showed lower levels when compared to controls (P = 0.0001). Analysis of single nucleotide polymorphisms in the FCN2 gene confirmed known influences on ficolin-2 serum levels, but did not support a genetic basis for the observed ficolin-2 deficiency in CVID. We found that CVID patients with bronchiectasis have very low levels of ficolin-2. The reason for the deficiency of ficolin-2 in CVID and any possible causal relationship is currently unknown. However, as bronchiectasis is a very important factor for morbidity and mortality in CVID, ficolin-2 could also serve as biomarker for monitoring disease complications such as bronchiectasis.
Assuntos
Bronquiectasia , Imunodeficiência de Variável Comum , Lectinas , Polimorfismo de Nucleotídeo Único , Biomarcadores/sangue , Bronquiectasia/sangue , Bronquiectasia/complicações , Bronquiectasia/genética , Bronquiectasia/mortalidade , Estudos de Coortes , Imunodeficiência de Variável Comum/sangue , Imunodeficiência de Variável Comum/complicações , Imunodeficiência de Variável Comum/genética , Imunodeficiência de Variável Comum/mortalidade , Feminino , Glicoproteínas/sangue , Glicoproteínas/genética , Humanos , Lectinas/sangue , Lectinas/genética , Masculino , FicolinasRESUMO
BACKGROUND: Intravenous sildenafil treatment has recently shown promising results and good tolerability in the treatment of refractory pulmonary hypertension (PH) in term and near-term neonates, while comparable data in preterm infants are still lacking. However, for critically ill preterm infants suffering from PH refractory to conventional treatment, sildenafil may represent a last treatment resort. PATIENTS AND METHODS: We reviewed the records of 6 critically ill extremely preterm infants who had suffered from PH refractory to conventional treatment and had obtained intravenous sildenafil after careful consideration as ultima ratio treatment. AIM: To describe the responses to sildenafil in terms of hemodynamic and respiratory changes during treatment and outcome. RESULTS: 4/6 patients showed resolution of severe PH with full reversal of ductal shunt direction into pure left-to-right shunt within 82 ± 35 h after sildenafil start. Remarkably, 2/6 patients developed pulmonary hemorrhage at a time point when significant improvement of PH had already taken place, both of them survived. Overall 4/6 patients died, two deaths were related to treatment-refractory PH. CONCLUSION: Intravenous sildenafil treatment seems effective in improving severe PH and hemodynamic instability in extremely preterm infants with refractory PH. Pulmonary hemorrhage may represent a distinct adverse effect of sildenafil treatment in these patients, presumably due to sudden reversal of ductal shunt. Accordingly, sildenafil should be restricted to most severe and refractory cases in this population.
Assuntos
Recém-Nascido de Peso Extremamente Baixo ao Nascer , Doenças do Prematuro/tratamento farmacológico , Síndrome da Persistência do Padrão de Circulação Fetal/tratamento farmacológico , Piperazinas/administração & dosagem , Sulfonamidas/administração & dosagem , Vasodilatadores/administração & dosagem , Feminino , Hemorragia/induzido quimicamente , Hemorragia/diagnóstico , Humanos , Recém-Nascido , Doenças do Prematuro/diagnóstico , Infusões Intravenosas , Unidades de Terapia Intensiva Neonatal , Pneumopatias/induzido quimicamente , Pneumopatias/diagnóstico , Masculino , Síndrome da Persistência do Padrão de Circulação Fetal/diagnóstico , Piperazinas/efeitos adversos , Pressão Propulsora Pulmonar/efeitos dos fármacos , Purinas/administração & dosagem , Purinas/efeitos adversos , Estudos Retrospectivos , Citrato de Sildenafila , Sulfonamidas/efeitos adversos , Vasodilatadores/efeitos adversosRESUMO
X-linked inhibitor of apoptosis (XIAP) deficiency caused by mutations in BIRC4 was initially described in patients with X-linked lymphoproliferative syndrome (XLP) who had no mutations in SH2D1A. In the initial reports, EBV-associated hemophagocytic lymphohistiocytosis (HLH) was the predominant clinical phenotype. Among 25 symptomatic patients diagnosed with XIAP deficiency, we identified 17 patients who initially presented with manifestations other than HLH. These included Crohn-like bowel disease (n=6), severe infectious mononucleosis (n=4), isolated splenomegaly (n=3), uveitis (n=1), periodic fever (n=1), fistulating skin abscesses (n=1) and severe Giardia enteritis (n=1). Subsequent manifestations included celiac-like disease, antibody deficiency, splenomegaly and partial HLH. Screening by flow cytometry identified 14 of 17 patients in our cohort. However, neither genotype nor protein expression nor results from cell death studies were clearly associated with the clinical phenotype. Only mutation analysis can reliably identify affected patients. XIAP deficiency must be considered in a wide range of clinical presentations.
Assuntos
Síndromes de Imunodeficiência/genética , Linfo-Histiocitose Hemofagocítica/genética , Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/deficiência , Adolescente , Adulto , Criança , Pré-Escolar , Genótipo , Humanos , Síndromes de Imunodeficiência/imunologia , Linfo-Histiocitose Hemofagocítica/imunologia , Masculino , Mutação , Células T Matadoras Naturais/imunologia , Fenótipo , Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/genética , Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X/imunologia , Adulto JovemRESUMO
Mutations in the TNFRSF13B gene, encoding TACI, have been found in common variable immunodeficiency (CVID) and selective IgA deficient (IgAD) patients, but only the association with CVID seems to be significant. In this study, Czech CVID, IgAD and primary hypo/dysgammaglobulinemic (HG/DG) patients were screened for all TNFRSF13B sequence variants. The TNFRSF13B gene was mutated in 4/70 CVID patients (5.7%), 9/161 IgAD patients (5.6%), 1/17 HG/DG patient (5.9%) and none of 195 controls. Eight different mutations were detected, including the most frequent p.C104R and p.A181E mutations as well as 1 novel missense mutation, p.R189K. A significant association of TNFRSF13B gene mutations was observed in both CVID (p=0.01) and IgAD (p=0.002) Czech patients. However, when combined with all published data, only the association with CVID remained significant compared with the controls (9.9% vs. 3.2%, p<10(-6)), while statistical significance disappeared for IgAD (5.7% vs. 3.2%, p=0.145). The silent mutation p.P97P was shown to be associated significantly with CVID compared with the controls in both Czech patients (allele frequency 4.3% vs. 0.2%, p=0.01) and in connection with the published data (5.1% vs. 1.8%, p=0.003). The relevance of some TNFRSF13B gene variants remains unclear and needs to be elucidated in future studies.
Assuntos
Imunodeficiência de Variável Comum/genética , Deficiência de IgA/genética , Mutação , Proteína Transmembrana Ativadora e Interagente do CAML/genética , População Branca/genética , Alelos , República Tcheca , Feminino , Frequência do Gene , Humanos , Íntrons , MasculinoRESUMO
Common variable immunodeficiency (CVID) is a primary immune disorder characterized by low immunoglobulin serum levels and increased susceptibility to infections. Underlying genetic causes are only known in less than 15% of patients and encompass mutations in the genes encoding for ICOS, TACI, BAFF-R, CD19, CD20, CD81 and MSH5. TACI is the most frequently mutated gene among CVID patients. We report on two pediatric Italian male siblings with hypogammaglobulinemia and recurrent respiratory and gastrointestinal infections in association with a novel compound heterozygous TACI mutation. Both patients carry the I87N/C104R mutation that has not been reported yet. This results in aberrant TACI expression and abrogates APRIL binding on EBV B cells. This study identifies a novel combined mutation in TNFRSF13B increasing the spectrum of TACI mutations associated with CVID.
Assuntos
Agamaglobulinemia/genética , Imunodeficiência de Variável Comum/genética , Infecções Respiratórias/genética , Proteína Transmembrana Ativadora e Interagente do CAML/genética , Agamaglobulinemia/complicações , Agamaglobulinemia/imunologia , Linfócitos B/imunologia , Linfócitos B/metabolismo , Linfócitos B/patologia , Sequência de Bases , Criança , Imunodeficiência de Variável Comum/complicações , Imunodeficiência de Variável Comum/imunologia , Genes Recessivos , Predisposição Genética para Doença , Heterozigoto , Humanos , Masculino , Dados de Sequência Molecular , Mutação , Linhagem , Infecções Respiratórias/complicações , Infecções Respiratórias/imunologia , Proteína Transmembrana Ativadora e Interagente do CAML/metabolismo , Membro 13 da Superfamília de Ligantes de Fatores de Necrose Tumoral/metabolismoRESUMO
Autoimmune lymphoproliferative syndrome (ALPS) is mainly caused by defects in the CD95 pathway. Raised CD3+TCRαß+CD4-CD8- double negative T cells and impaired T cell apoptosis are hallmarks of the disease. In contrast, the B cell compartment has been less well studied. We found an altered distribution of B cell subsets with raised transitional B cells and reduced marginal zone B cells, switched memory B cells and plasma blasts in most of 22 analyzed ALPS patients. Moreover, 5 out of 66 ALPS patients presented with low IgG and susceptibility to infection revealing a significant overlap between ALPS and common variable immunodeficiency (CVID). In patients presenting with lymphoproliferation, cytopenia, hypogammaglobulinemia and impaired B cell differentiation, serum biomarkers were helpful in addition to apoptosis tests for the identification of ALPS patients. Our observations may indicate a role for apoptosis defects in some diseases currently classified as CVID.
Assuntos
Síndrome Linfoproliferativa Autoimune/diagnóstico , Síndrome Linfoproliferativa Autoimune/imunologia , Linfócitos B/imunologia , Imunodeficiência de Variável Comum/diagnóstico , Imunodeficiência de Variável Comum/imunologia , Proteína Ligante Fas/sangue , Interleucina-10/sangue , Vitamina B 12/sangue , Adolescente , Adulto , Agamaglobulinemia/imunologia , Apoptose , Biomarcadores/sangue , Criança , Pré-Escolar , Diagnóstico Diferencial , Proteína Ligante Fas/imunologia , Citometria de Fluxo , Humanos , Imunoglobulina G/sangue , Interleucina-10/imunologia , Pessoa de Meia-Idade , Monócitos/imunologia , Fenótipo , Linfócitos T/imunologia , Vitamina B 12/imunologia , Receptor fas/sangue , Receptor fas/imunologiaAssuntos
Anticorpos Monoclonais/uso terapêutico , Antirreumáticos/uso terapêutico , Doenças em Gêmeos/tratamento farmacológico , Rigidez Muscular Espasmódica/tratamento farmacológico , Tireoidite Autoimune/complicações , Adulto , Anticorpos Monoclonais Murinos , Estudos Cross-Over , Método Duplo-Cego , Humanos , Masculino , Rituximab , Falha de Tratamento , Gêmeos MonozigóticosRESUMO
Mannose-binding lectin (MBL), activating protein of the lectin pathway of the complement system, is an important component of the non-specific immune response. MBL2 gene polymorphisms, both in the coding and promoter regions, lead to low or deficient serum MBL levels. Low serum MBL levels were shown to be associated with serious infectious complications, mainly in patients in whom other non-specific immune system barriers were disturbed (granulocytopenia, cystic fibrosis). We have analysed two promoter (-550 and -221) and three exon (codons 52, 54 and 57) MBL2 polymorphisms in a total of 94 patients with common variable immunodeficiency (CVID) from two immunodeficiency centres. Low-producing genotypes were associated with the presence of bronchiectasis (P = 0.009), lung fibrosis (P = 0.037) and also with respiratory insufficiency (P = 0.029). We could not demonstrate any association of MBL deficiency with age at onset of clinical symptoms, age at diagnosis, the number of pneumonias before diagnosis or serum immunoglobulin (Ig)G, IgA and IgM levels before initiation of Ig treatment. No association with emphysema development was observed, such as with lung function test abnormalities. No effect of MBL2 genotypes on the presence of diarrhoea, granuloma formation, lymphadenopathy, splenomegaly, frequency of respiratory tract infection or the number of antibiotic courses of the patients was observed. Our study suggests that low MBL-producing genotypes predispose to bronchiectasis formation, and also fibrosis and respiratory insufficiency development, but have no effect on other complications in CVID patients.
Assuntos
Imunodeficiência de Variável Comum/genética , Pneumopatias/genética , Lectina de Ligação a Manose/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Imunodeficiência de Variável Comum/complicações , República Tcheca , Feminino , Predisposição Genética para Doença , Alemanha , Humanos , Imunoglobulinas/sangue , Masculino , Pessoa de Meia-Idade , Polimorfismo Genético , Adulto JovemRESUMO
Active vaccination of CVID patients with standard vaccines has rarely been studied in depth although some patients have been shown to develop transient vaccine-specific immunity. We addressed the question whether these patients can be identified by functional classification of their B cell subsets in vitro. Twenty-one CVID patients receiving regular IgG substitution were immunized with anti-peptide and anti-polysaccharide vaccines. Humoral vaccination responses were compared to the numbers of circulating memory B cells, CD21(low) B cells and the capacity to produce antibodies in vitro. Our findings allow four conclusions: (1) positive vaccination responses are not contradictory to the diagnosis of CVID; they occurred against polypeptide vaccines in 23% and against polysaccharide antigens in 18% of all vaccinations. (2) Class-switched antibody responses occur preferentially in patients of CVID group II. (3) A normal percentage of IgM memory B cells is necessary but not sufficient for a vaccination response to polysaccharide antigens. (4) Active vaccination in addition to IgG replacement therapy should be performed in patients of CVID type II - especially in case of vaccines for which passive protection cannot be guaranteed.
Assuntos
Linfócitos B/imunologia , Imunodeficiência de Variável Comum/imunologia , Peptídeos/uso terapêutico , Polissacarídeos/uso terapêutico , Vacinação , Adulto , Idoso , Formação de Anticorpos/imunologia , Linfócitos B/classificação , Imunodeficiência de Variável Comum/classificação , Imunodeficiência de Variável Comum/terapia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Memória Imunológica , Subpopulações de Linfócitos/classificação , Subpopulações de Linfócitos/imunologia , Masculino , Pessoa de Meia-Idade , Vacinas Pneumocócicas/uso terapêutico , Streptococcus pneumoniae/imunologiaRESUMO
No disponible
No disponible
Assuntos
Humanos , Agamaglobulinemia/genética , Antígenos CD/genética , Antígenos CD19/análise , Fator Ativador de Células B/deficiência , Proteína Transmembrana Ativadora e Interagente do CAML/deficiênciaRESUMO
No disponible
No disponible
Assuntos
Humanos , Agamaglobulinemia/genética , Antígenos CD19 , Carcinoma de Células de Transição/imunologia , Síndromes de Imunodeficiência/imunologiaRESUMO
The functional interaction of BAFF and APRIL with TNF receptor superfamily members BAFFR, TACI and BCMA is crucial for development and maintenance of humoral immunity in mice and humans. Using a candidate gene approach, we identified homozygous and heterozygous mutations in TNFRSF13B, encoding TACI, in 13 individuals with common variable immunodeficiency. Homozygosity with respect to mutations causing the amino acid substitutions S144X and C104R abrogated APRIL binding and resulted in loss of TACI function, as evidenced by impaired proliferative response to IgM-APRIL costimulation and defective class switch recombination induced by IL-10 and APRIL or BAFF. Family members heterozygous with respect to the C104R mutation and individuals with sporadic common variable immunodeficiency who were heterozygous with respect to the amino acid substitutions A181E, S194X and R202H had humoral immunodeficiency. Although signs of autoimmunity and lymphoproliferation are evident, the human phenotype differs from that of the Tnfrsf13b-/- mouse model.
Assuntos
Imunodeficiência de Variável Comum/genética , Proteínas de Membrana/genética , Mutação , Receptores do Fator de Necrose Tumoral/genética , Sequência de Aminoácidos , Formação de Anticorpos , Divisão Celular/genética , Divisão Celular/fisiologia , Feminino , Homozigoto , Humanos , Imunoglobulina M/fisiologia , Masculino , Proteínas de Membrana/química , Dados de Sequência Molecular , Proteínas Nucleares/genética , Proteínas Nucleares/fisiologia , Linhagem , Receptores do Fator de Necrose Tumoral/química , Proteína Transmembrana Ativadora e Interagente do CAMLRESUMO
Different individuals with the same kind of primary immunodeficiency may start having symptoms from early childhood on, or alternatively much later in adult life, or never. The differences in phenotype can only partly be deduced from genotype-analysis or--in case of female patients with X-linked diseases--from age-related skewing of lyonisation. The role of compensatory immune mechanisms is less clear. The microbial spectrum of infections is usually the same for both adult and infantile forms of a special primary immunodeficiency syndrome. Yet, many of the adult forms are associated with non-infectious complications, such as granuloma formation, autoimmunity or tumors. Besides standard antibiotic treatment and IgG replacement therapy, there are now different cytokine- or enzyme-replacement regimens available for some of the primary immunodeficiencies. However, exact diagnostic classification of the immunodeficiency should be obtained before such treatment modalities are used. Adult primary immunodeficiency syndromes therefore represent a challenge to both clinicians and molecular biologists.
Assuntos
Síndromes de Imunodeficiência/diagnóstico , Síndromes de Imunodeficiência/genética , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Cromossomos Humanos X , Imunodeficiência de Variável Comum/diagnóstico , Imunodeficiência de Variável Comum/genética , Imunodeficiência de Variável Comum/imunologia , Feminino , Genótipo , Humanos , Síndromes de Imunodeficiência/imunologia , Masculino , Pessoa de Meia-Idade , Infecções Oportunistas/diagnóstico , Infecções Oportunistas/genética , Infecções Oportunistas/imunologia , Fenótipo , Prognóstico , Aberrações dos Cromossomos SexuaisRESUMO
Common variable immunodeficiency (CVID, OMIM 240500) and selective immunoglobulin A deficiency (IgAD) are the most frequent primary immunodeficiencies in humans. Of the cases with CVID/IgAD, 20%-25% are familial, but the only previous claims of linkage or association are to the HLA region on chromosome 6p. We report the results of a genome-wide scan in three multiplex families with CVID, IgAD, and dysgammaglobulinemia, where affection is inherited in an autosomal dominant pattern. Two of the families are consistent with linkage to the telomeric region of chromosome 5p, whereas the third is consistent with linkage to the HLA region. Using a locus heterogeneity model and a conservative penetrance model, we obtained a LOD score of 3.35 for the 5p region. We sequenced the exons of one promising candidate gene within this region (PDCD6, also known as ALG-2) but found no causative mutation.
Assuntos
Cromossomos Humanos Par 5/genética , Imunodeficiência de Variável Comum/genética , Ligação Genética , Marcadores Genéticos/genética , Polimorfismo Genético , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Cromossomos Humanos Par 6/genética , Feminino , Heterogeneidade Genética , Ligação Genética/genética , Genótipo , Humanos , Escore Lod , Masculino , Pessoa de Meia-Idade , LinhagemRESUMO
Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents, have a low buoyant density, and preferentially contain lipid-modified proteins, like glycosyl phosphatidylinositol (GPI)-anchored proteins. The lipid rafts were isolated from human erythrocytes and major protein components were identified. Apart from the GPI-anchored proteins, the most abundant integral proteins were found to be the distantly related membrane proteins stomatin (band 7.2b), flotillin-1, and flotillin-2. Flotillins, already described as lipid raft components in neurons and caveolae-associated proteins in A498 kidney cells, have not been recognized as red cell components yet. In addition, it was shown that the major cytoskeletal proteins, spectrin, actin, band 4.1, and band 4.2, are partly associated with the lipid rafts. Stomatin and the flotillins are present as independently organized high-order oligomers, suggesting that these complexes act as separate scaffolding components at the cytoplasmic face of erythrocyte lipid rafts.
Assuntos
Proteínas Sanguíneas/análise , Proteínas do Citoesqueleto/sangue , Membrana Eritrocítica/química , Microdomínios da Membrana/química , Proteínas de Membrana/sangue , Anemia Hemolítica Congênita/sangue , Anemia Hemolítica Congênita/genética , Detergentes/farmacologia , Membrana Eritrocítica/efeitos dos fármacos , Membrana Eritrocítica/ultraestrutura , Eritrócitos Anormais/química , Eritrócitos Anormais/efeitos dos fármacos , Eritrócitos Anormais/ultraestrutura , Humanos , Substâncias Macromoleculares , Octoxinol/farmacologia , SolubilidadeRESUMO
Follicle-stimulating hormone is the major regulator of growth and development of antral follicles in the ovary. Granulosa cells (GCs) in these follicles are coupled via gap junctions (GJs) consisting of connexin 43 (Cx 43). Because we and others have found that Cx 43 and GJs, respectively, are more abundant in large antral follicles compared with small antral and preantral follicles, we hypothesized that FSH may control Cx 43 gene expression, GJ formation, and intercellular communication. To directly address these points, we chose a rat GC line (GFSHR-17) expressing the FSH receptor and the Cx 43 gene. The functionality of FSH receptors was shown by the effects of porcine FSH, namely cell rounding, reduced cellular proliferation, and stimulation of progesterone production of GFSHR-17 cells, which are effects that were detectable within hours. Treatment with FSH also statistically significantly increased Cx 43 mRNA levels, as shown after 6 to 9 h in Northern blots. These effects were antedated by altered GJ communication, which was observed within seconds. Using a single-cell/whole-cell patch clamp technique, we showed that FSH rapidly and reversibly enhanced electrical cell coupling of GFSHR-17 cells. Increased GJ communication was associated with statistically significantly decreased phosphorylation of Cx 43, which was observed within 10 min after FSH addition, during immunoprecipitation experiments. Our results demonstrate, to our knowledge for the first time, that the gonadotropin FSH acutely and directly stimulates intercellular communication of GFSHR-17 cells through existing GJs. Moreover, FSH also increases levels of Cx 43 mRNA. These changes are associated with reduced proliferation and enhanced differentiation of GFSHR-17 cells. In vivo factors in addition to FSH may be involved in the regulation of GJ/GJ communication between GCs in the follicle, but our results suggest that improved cell-to-cell coupling, enhanced Cx 43 gene expression, and possibly, formation of new GJs are direct consequences of FSH receptor activation and may antedate and/or initiate the pivotal effects of FSH on GCs.
Assuntos
Comunicação Celular/efeitos dos fármacos , Conexina 43/biossíntese , Hormônio Foliculoestimulante/farmacologia , Junções Comunicantes/efeitos dos fármacos , Células da Granulosa/metabolismo , Animais , Northern Blotting , Western Blotting , Divisão Celular/fisiologia , Linhagem Celular , Conexina 43/genética , Eletrofisiologia , Feminino , Imunofluorescência , Células da Granulosa/efeitos dos fármacos , Hibridização In Situ , Técnicas de Patch-Clamp , Testes de Precipitina , Ratos , Ratos Sprague-DawleyRESUMO
The 40 kDa erythrocyte membrane protein p40/GPR69A, previously assigned to the G-protein-coupled receptor superfamily, was now identified by peptide-antibodies and characterized as a loosely associated peripheral membrane protein. This result is in striking contrast to the proposed seven-transmembrane protein structure and function and therefore we wish to correct our previous proposal. p40 is located at the cytoplasmic side of the membrane and is neither associated with the cytoskeleton nor lipid rafts. Refined sequence analysis revealed that p40 is related to the LanC family of bacterial membrane-associated proteins which are involved in the biosynthesis of antimicrobial peptides. Therefore, we rename p40 to LanC-like protein 1 (LANCL1) and suggest that it may play a similar role as a peptide-modifying enzyme component in eukaryotic cells.